2,332 research outputs found
Computation with narrow CTCs
We examine some variants of computation with closed timelike curves (CTCs),
where various restrictions are imposed on the memory of the computer, and the
information carrying capacity and range of the CTC. We give full
characterizations of the classes of languages recognized by polynomial time
probabilistic and quantum computers that can send a single classical bit to
their own past. Such narrow CTCs are demonstrated to add the power of limited
nondeterminism to deterministic computers, and lead to exponential speedup in
constant-space probabilistic and quantum computation. We show that, given a
time machine with constant negative delay, one can implement CTC-based
computations without the need to know about the runtime beforehand.Comment: 16 pages. A few typo was correcte
Accreditation of the PGD laboratory
Accreditation according to an internationally recognized standard is increasingly acknowledged as the single most effective route to comprehensive laboratory quality assurance, and many countries are progressively moving towards compulsory accreditation of medical testing laboratories. The ESHRE PGD Consortium and some regulatory bodies recommend that all PGD laboratories should be accredited or working actively towards accreditation, according to the internationally recognized standard ISO 15189, ‘Medical laboratories—Particular requirements for quality and competence'. ISO 15189 requires comprehensive quality assurance. Detailed management and technical requirements are defined in the two major chapters. The management requirements address quality management including the quality policy and manual, document control, non-conformities and corrective actions, continual improvement, auditing, management review, contracts, referrals and resolution of complaints. Technical requirements include personnel competence (both technical and medical), equipment, accommodation and environment, and pre-analytical, analytical and post-analytical processes. Emphasis is placed on the particular requirements of patient care: notably sample identification and traceability, test validation and interpretation and reporting of results. Quality indicators must be developed to monitor contributions to patient care and continual improvement. We discuss the implementation of ISO 15189 with a specific emphasis on the PGD laboratory, highlight elements of particular importance or difficulty and provide suggestions of effective and efficient ways to obtain accreditation. The focus is on the European environment although the principles are globally applicabl
How do you solve a problem like Maria? Family complexity and institutional complications in UK social work
This paper is based on one part of an international comparative project, funded by
NORFACE (New Opportunities for Research Funding Agency Co-operation in Europe
‘Welfare State Futures’), involving eight countries: Bulgaria, Chile, Ireland, Lithuania,
Mexico, Norway, UK, and Sweden. The Welfare State Futures programme seeks to stimulate
multi-disciplinary and comparative research to ‘rethink’ welfare states by asking and
answering fundamental questions about how they are designed, delivered and experienced
in the 21st century. The broad research questions addressed are: if and how social workers’
conceptions and/or definitions of family impact on the way social workers engage with
complex families; If and how social policies that frame social work context impact on the
way social workers engage with complex families. Discussion here draws on data from 24
focus groups held in England, Wales, Northern Ireland and Scotland
Human airway smooth muscle maintain in situ cell orientation and phenotype when cultured on aligned electrospun scaffolds
Human airway smooth muscle (HASM) contraction plays a central role in regulating airway resistance in both healthy and asthmatic bronchioles. In vitro studies that investigate the intricate mechanisms that regulate this contractile process are predominantly conducted on tissue culture plastic, a rigid, 2D geometry, unlike the 3D microenvironment smooth muscle cells are exposed to in situ. It is increasingly apparent that cellular characteristics and responses are altered between cells cultured on 2D substrates compared with 3D topographies. Electrospinning is an attractive method to produce 3D topographies for cell culturing as the fibers produced have dimensions within the nanometer range, similar to cells' natural environment. We have developed an electrospun scaffold using the nondegradable, nontoxic, polymer polyethylene terephthalate (PET) composed of uniaxially orientated nanofibers and have evaluated this topography's effect on HASM cell adhesion, alignment, and morphology. The fibers orientation provided contact guidance enabling the formation of fully aligned sheets of smooth muscle. Moreover, smooth muscle cells cultured on the scaffold present an elongated cell phenotype with altered contractile protein levels and distribution. HASM cells cultured on this scaffold responded to the bronchoconstrictor bradykinin. The platform presented provides a novel in vitro model that promotes airway smooth muscle cell development toward a more in vivo-like phenotype while providing topological cues to ensure full cell alignment
A fluorescent assay for ceramide synthase activity
The sphingolipids are a diverse family of lipids with important roles in membrane compartmentalization, intracellular signaling, and cell-cell recognition. The central sphingolipid metabolite is ceramide, formed by the transfer of a variable length fatty acid from coenzyme A to a sphingoid base, generally sphingosine or dihydrosphingosine (sphinganine) in mammals. This reaction is catalyzed by a family of six ceramide synthases (CerS1-6). CerS activity is usually assayed using either radioactive substrates or LC-MS/MS. We describe a CerS assay with fluorescent, NBD-labeled sphinganine as substrate. The assay is readily able to detect endogenous CerS activity when using amounts of cell or tissue homogenate protein that are lower than those reported for the radioactive assay, and the Michaelis-Menten constant was essentially the same for NBD-sphinganine and unlabeled sphinganine, indicating that NBD-sphinganine is a good substrate for these enzymes. Using our assay, we confirm that the new clinical immunosuppressant FTY720 is a competitive inhibitor of CerS activity, and show that inhibition requires the compound's lipid tail and amine headgroup. In summary, we describe a fluorescent assay for CerS activity that circumvents the need to use radioactive substrates, while being more accessible and cheaper than LC-MS based assays.Hyun Joon Kim, Qiao Qiao, Hamish D. Toop, Jonathan C. Morris and Anthony S. Do
Minority-carrier transport in InGaAsSb thermophotovoltaic diodes
Uncoated InGaAsSb/GaSb thermophotovoltaic (TPV) diodes with 0.56 eV (2.2 {micro}m) bandgaps exhibit external quantum efficiencies of 59% at 2 {micro}m. The devices have electron diffusion lengths as long as 29 {micro}m in 8-{micro}m-wide p-InGaAsSb layers and hole diffusion lengths of 3 {micro}m in 6-{micro}m-wide n-InGaAsSb layers. The electron and hole diffusion lengths appear to increase with increasing p- and n-layer widths. At 632.8 nm the internal quantum efficiencies of diodes with 1- to 8-{micro}m-wide p-layers are above 89% and are independent of the p-layer width, indicating long electron diffusion lengths. InGaAsSb has, therefore, excellent minority carrier transport properties that are well suited to efficient TPV diode operation. The structures were grown by molecular-beam epitaxy
Mean flow and spiral defect chaos in Rayleigh-Benard convection
We describe a numerical procedure to construct a modified velocity field that
does not have any mean flow. Using this procedure, we present two results.
Firstly, we show that, in the absence of mean flow, spiral defect chaos
collapses to a stationary pattern comprising textures of stripes with angular
bends. The quenched patterns are characterized by mean wavenumbers that
approach those uniquely selected by focus-type singularities, which, in the
absence of mean flow, lie at the zig-zag instability boundary. The quenched
patterns also have larger correlation lengths and are comprised of rolls with
less curvature. Secondly, we describe how mean flow can contribute to the
commonly observed phenomenon of rolls terminating perpendicularly into lateral
walls. We show that, in the absence of mean flow, rolls begin to terminate into
lateral walls at an oblique angle. This obliqueness increases with Rayleigh
number.Comment: 14 pages, 19 figure
Wormholes and Ringholes in a Dark-Energy Universe
The effects that the present accelerating expansion of the universe has on
the size and shape of Lorentzian wormholes and ringholes are considered. It is
shown that, quite similarly to how it occurs for inflating wormholes, relative
to the initial embedding-space coordinate system, whereas the shape of the
considered holes is always preserved with time, their size is driven by the
expansion to increase by a factor which is proportional to the scale factor of
the universe. In the case that dark energy is phantom energy, which is not
excluded by present constraints on the dark-energy equation of state, that size
increase with time becomes quite more remarkable, and a rather speculative
scenario is here presented where the big rip can be circumvented by future
advanced civilizations by utilizing sufficiently grown up wormholes and
ringholes as time machines that shortcut the big-rip singularity.Comment: 11 pages, RevTex, to appear in Phys. Rev.
Genome-wide enrichment analysis between endometriosis and obesity-related traits reveals novel susceptibility loci
Endometriosis is a chronic inflammatory condition in women that results in pelvic pain and subfertility, and has been associated with decreased body mass index (BMI). Genetic variants contributing to the heritable component have started to emerge from genome-wide association studies (GWAS), although the majority remain unknown. Unexpectedly, we observed an intergenic locus on 7p15.2 that was genome-wide significantly associated with both endometriosis and fat distribution (waist-to-hip ratio adjusted for BMI; WHRadjBMI) in an independent meta-GWAS of European ancestry individuals. This led us to investigate the potential overlap in genetic variants underlying the aetiology of endometriosis, WHRadjBMI and BMI using GWAS data. Our analyses demonstrated significant enrichment of common variants between fat distribution and endometriosis (P = 3.7 × 10(-3)), which was stronger when we restricted the investigation to more severe (Stage B) cases (P = 4.5 × 10(-4)). However, no genetic enrichment was observed between endometriosis and BMI (P = 0.79). In addition to 7p15.2, we identify four more variants with statistically significant evidence of involvement in both endometriosis and WHRadjBMI (in/near KIFAP3, CAB39L, WNT4, GRB14); two of these, KIFAP3 and CAB39L, are novel associations for both traits. KIFAP3, WNT4 and 7p15.2 are associated with the WNT signalling pathway; formal pathway analysis confirmed a statistically significant (P = 6.41 × 10(-4)) overrepresentation of shared associations in developmental processes/WNT signalling between the two traits. Our results demonstrate an example of potential biological pleiotropy that was hitherto unknown, and represent an opportunity for functional follow-up of loci and further cross-phenotype comparisons to assess how fat distribution and endometriosis pathogenesis research fields can inform each other
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