Influence of a Maternal Dietary Yeast Supplement on Immunoglobulin Concentrations in Foals from Birth to Four Months of Age

Agriculture/Environmental Science: 2nd Place (The Ohio State University Denman Undergraduate Research Forum)Previous studies in multiple species have shown that maternal diet can affect immunoglobulin concentrations in their resulting offspring. To our knowledge, the effect of maternal dietary yeast supplementation on immunoglobulin levels in foals has not been studied. In this study eight Quarter Horse mares (14.5 ± 7.5 yr) were randomly assigned to one of two groups: Yeast or Control. All mares received a control diet of 0.5% BW of a 16% CP pelleted concentrate with water and mixed grass hay ad libitum. Mares in the yeast treatment group also received 1g/45.4 kg of BW/d of a live culture of Saccharomyces cerevisiae from 250 d of gestation to 90 d post-foaling. All mares were vaccinated at d 300 of gestation against Eastern and Western equine encephalomyelitis, equine rhinopneumonitis (EHV-1 and EHV-4), equine influenza (type A2), tetanus and West Nile virus. Blood samples were collected from the foals via jugular venipuncture immediately after parturition (d 0), at 12 and 24 hr and 30, 60, 90, and 120 d post-foaling. Sera samples were analyzed for total IgG including IgGa, IgGb, and IgG(T), as well as IgA, IgM, and IgE concentrations using commercial ELISA kits. Data were analyzed using PROC MIXED of SAS and a p-value of ≤ 0.05 was considered statistically significant. Supplementing the maternal diet with live yeast did not influence foal IgGa, IgGb, IgA, IgM, or IgE concentrations. However, IgG(T) concentrations were significantly higher (P = 0.0063) on d 60 post-foaling in foals born from mares fed the yeast supplement compared to controls. Overall, maternal dietary yeast supplementation during late gestation and early lactation did not influence immunoglobulin concentrations in their foals.Academic Major: Animal Science

The Selectivity and Functional Connectivity of the Anterior Temporal Lobes

One influential account asserts that the anterior temporal lobe (ATL) is a domain-general hub for semantic memory. Other evidence indicates it is part of a domain-specific social cognition system. Arbitrating these accounts using functional magnetic resonance imaging has previously been difficult because of magnetic susceptibility artifacts in the region. The present study used parameters optimized for imaging the ATL, and had subjects encode facts about unfamiliar people, buildings, and hammers. Using both conjunction and region of interest analyses, person-selective responses were observed in both the left and right ATL. Neither building-selective, hammer-selective nor domain-general responses were observed in the ATLs, although they were observed in other brain regions. These findings were supported by “resting-state” functional connectivity analyses using independent datasets from the same subjects. Person-selective ATL clusters were functionally connected with the brain's wider social cognition network. Rather than serving as a domain-general semantic hub, the ATLs work in unison with the social cognition system to support learning facts about others

Immunogenicity of a 26-Valent Group A Streptococcal Vaccine

A multivalent vaccine containing amino-terminal M protein fragments from 26 different serotypes of group A streptococci was constructed by recombinant techniques. The vaccine consisted of four different recombinant proteins that were formulated with alum to contain 400 μg of protein per dose. Rabbits were immunized via the intramuscular route at 0, 4, and 16 weeks. Immune sera were assayed for the presence of type-specific antibodies against the individual recombinant M peptides by enzyme-linked immunosorbent assay and for opsonic antibodies by in vitro opsonization tests and indirect bactericidal tests. The 26-valent vaccine was highly immunogenic and elicited fourfold or greater increases in antibody levels against 25 of the 26 serotypes represented in the vaccine. The immune sera were broadly opsonic and were bactericidal against the majority of the 26 different serotypes. Importantly, none of the immune sera cross-reacted with human tissues. Our results indicate that type-specific, protective M protein epitopes can be incorporated into complex, multivalent vaccines designed to elicit broadly protective opsonic antibodies in the absence of tissue-cross-reactive antibodies

Intranasal Immunization with Multivalent Group A Streptococcal Vaccines Protects Mice against Intranasal Challenge Infections

We have previously shown that a hexavalent group A streptococcal M protein-based vaccine evoked bactericidal antibodies after intramuscular injection. In the present study, we show that the hexavalent vaccine formulated with several different mucosal adjuvants and delivered intranasally induced serum and salivary antibodies that protected mice from intranasal challenge infections with virulent group A streptococci. The hexavalent vaccine was formulated with liposomes with or without monophosphorylated lipid A (MPL), cholera toxin B subunit with or without holotoxin, or proteosomes from Neisseria meningitidis outer membrane proteins complexed with lipopolysaccharide from Shigella flexneri. Intranasal immunization with the hexavalent vaccine mixed with these adjuvants resulted in significant levels of antibodies in serum 2 weeks after the final dose. Mean serum antibody titers were equivalent in all groups of mice except those that were immunized with hexavalent protein plus liposomes without MPL, which were significantly lower. Salivary antibodies were also detected in mice that received the vaccine formulated with the four strongest adjuvants. T-cell proliferative assays and cytokine assays using lymphocytes from cervical lymph nodes and spleens from mice immunized with the hexavalent vaccine formulated with proteosomes indicated the presence of hexavalent protein-specific T cells and a Th1-weighted mixed Th1-Th2 cytokine profile. Intranasal immunization with adjuvanted formulations of the hexavalent vaccine resulted in significant levels of protection (80 to 100%) following intranasal challenge infections with type 24 group A streptococci. Our results indicate that intranasal delivery of adjuvanted multivalent M protein vaccines induces protective antibody responses and may provide an alternative to parenteral vaccine formulations

Immunogenicity of Synthetic Peptides related to the core Peptide sequence encoded by the human MUC1 Mucine gene: Effect of Immunization on the growth of Murine Mammary Adenocarcinoma cells transfected with the human MUC1 gene. J. Cancer Immunol

The immune response of CAF1 mice to various synthetic peptides (SP) related to the amino acid sequence (PDTRPAPGSTAPPAHGVTSA) of the tandem repeat of the MUC1 human breast mucin core peptide was evaluated. The most immunogenic preparations of the synthetic peptides were those conjugated to keyhole limpet hemocyanin (KLH) or clustered in a dendritic multiple antigenic peptide (MAP-4) configuration. The mice were immunized subcutaneously with synthetic peptides emulsified in RIBI adjuvant, employing various immunization protocols. Equivalently high IgG responses were induced using SP-KLH conjugates (GVTSAPDTRPAPGSTA-KLH) or an SP--MAP-4 chimeric configuration (SP1-6), which also included a universal malarial CST-3 T-helper epitope (SP1-6 = SAPDTRPAEKKIAKMEKASSVFNVVNS--MAP-4). These IgG antibodies bound both the appropriate MUC1 synthetic peptides and the cell surface expressed MUC1 mucin on murine mammary cells that had been transfected with the human MUC1 gene and a human breast cancer cell line that expresses cell-surface MUC1. A MAP-4 molecule, which included the entire 20-amino-acid sequence of the MUC1 tandem repeat (SP1-5 = PDTRPAPGSTAPPAHGVTSA-MAP-4) induced a poor IgG response. In contrast, all three types of molecule: SP-KLH, SP1-6 and SP1-5, were found to be good immunogens for the induction of specific delayed-type hypersensitivity (DTH) reactions measured using either synthetic peptides or MUC1-transfected cells. In addition, immunization with irradiated MUC1-transfected cells induced strong DTH reactions measured using synthetic peptides that expressed the PDTRP sequence, which has been shown to be, or to overlap, a T cell epitope in humans and a B cell epitope in mice. Finally, it was demonstrated that synthetic MUC1 peptide vaccines could be used both prophylactically and therapeutically to inhibit the growth of MUC1-transfected tumor cells and prolong the survival of tumor-bearing mice

Explaining the rise of moralizing religions : a test of competing hypotheses using the Seshat Databank

The causes, consequences, and timing of the rise of moralizing religions in world history have been the focus of intense debate. Progress has been limited by the availability of quantitative data to test competing theories, by divergent ideas regarding both predictor and outcomes variables, and by differences of opinion over methodology. To address all these problems, we utilize Seshat: Global History Databank, a large storehouse of information designed to test theories concerning the evolutionary drivers of social complexity. In addition to the Big Gods hypothesis, which proposes that moralizing religion contributed to the success of increasingly large-scale complex societies, we consider the role of warfare, animal husbandry, and agricultural productivity in the rise of moralizing religions. Using a broad range of new measures of belief in moralizing supernatural punishment, we find strong support for previous research showing that such beliefs did not drive the rise of social complexity. By contrast, our analyses indicate that intergroup warfare, supported by resource availability, played a major role in the evolution of both social complexity and moralizing religions. Thus, the correlation between social complexity and moralizing religion seems to result from shared evolutionary drivers, rather than from direct causal relationships between these two variables