Protective effect of bixin on cisplatin-induced genotoxicity in PC12 cells

Bixin is the main carotenoid found in annatto seeds (Bixa orellana L.) and is responsible for their reddish-orange color. The antioxidant properties of this compound are associated with its ability to scavenge free radicals, which may reduce damage and protect tissues against toxicity caused by anticancer drugs such as cisplatin. In this study, the genotoxicity and antigenotoxicity of bixin on cisplatin-induced toxicity in PC12 cells was assessed. Cytotoxicity was evaluated using the mu assay, mutagenicity, genotoxicity, and protective effect of bixin were evaluated using the micronucleus test and comet assay. PC12 cells were treated with bixin (0.05, 0.08, and 0.10 mu g/mL), cisplatin (0.1 mu g/mL) or a combination of both bixin and cisplatin. Bixin was neither cytotoxic nor genotoxic compared to the controls. In the combined treatment bixin significantly reduced the percentage of DNA in tail and the frequency of micronuclei induced by cisplatin. This result suggests that bixin can function as a protective agent, reducing cisplatin-induced DNA damage in PC12 cells, and it is possible that this protection could also extend to neuronal cells. Further studies are being conducted to better understand the mechanisms involved in the activity of this protective agent prior to using it therapeutically. (C) 2011 Elsevier Ltd. All rights reserved.CNPq [470214/2008-2]CNP

Evaluation of curcumin and cisplatin-induced DNA damage in PC12 cells by the alkaline comet assay

A very appropriate method for antigenotoxicity evaluation of antioxidants is the comet assay, since this analytical method detects initial DNA lesions that are still subject to repair; in other words, lesions that are very associated to damages resulting from the generation and subsequent action of reactive species. However, a solid evaluation should be developed in order to avoid inexact interpretations. In our study, besides the association of curcumin with cisplatin, curcumin and cisplatin agents were also tested separately. Classical genotoxic compounds, when tested by the comet assay, present an increase in the nucleoid tail; however, the cisplatin treatment has resulted in a decrease of DNA migration. This was an expected effect, as the cross-links between cisplatin and DNA decrease the DNA electrophoretic mobility. A similar effect was observed with the curcumin treatment, which decreased the nucleoid tail. Such effect was not expected and reinforced the necessity of including in the study, separate treatment groups with potentially antigenotoxic substances. The comet assay results have been analyzed using specific software for image analysis, as well as the classical visual analysis, and we have observed that the effect of decrease in DNA electrophoretic mobility was more easily observed when the data were analyzed by the software.CAPESFAPESP (Fundacao de Pesquisa do Estado de Sao Paulo)[08/53947-7]CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico

Atlas de Suelos de America Latina y El Caribe

A necessary starting point to achieve the objective of preserving soil resources is to reach an adequate level of knowledge on their status and to raise awareness on their importance (UNEP, 2007; Sanchez et al., 2009; Palm et al., 2010; Sachs et al., 2010). In order to improve communication and to raise soil awareness of the public at large, stakeholders, policy makers, and other scientists to the importance of soil in Latin America, the Joint Research Centre of the European Commission is producing the first ever Soil Atlas of Latin America and Caribbean (LAC). The Atlas compile existing information on different soil types as easily understandable maps (both at regional and continental scale) covering the continents. The Soil Atlas of LAC illustrates the diversity of soils from the humid tropics to the arid deserts through a series of maps supported by explanatory texts, high quality photographs and descriptive graphics. Supporting texts describe the major soil types, together with their principal characteristics and the main soil forming processes. This Atlas, as one of the outcomes of Euroclima project, will have a strong emphasis on Climate Change. The soil maps showed in the Atlas will be based on the Soterlac 1:5,000,000, that will be updated and validated on the base of the information provided by the LAC countries. Soils will be discussed both at regional scale, on the base of Ecozones, and at national scale. A specific section will be devoted to ethnopedologia. Together with the publication of the Atlas, the soil map and associated datasets on soil characteristics will be made freely available. These datasets will be useful for making broad distinction among soil types and provide general trends at the global and regional scales. The datasets will be made accessible for free downloading from the portals of the SOIL Action (http://eusoils.jrc.ec.europa.eu/) The format of the Atlas will allows an accessible, user-friendly approach avoiding the more traditional focus on soil classification and soil survey interpretation. Furthermore other products aimed to raise awareness on soil, have also been produced, such as the Soil Calendar of Latin America and Caribbean (2012). The Atlases link the theme of soil with rural development and, at the same time, supports the goals of the EU Thematic Strategy for Soil Protection in conserving a threatened natural resource that is vital to human existence. Not only climate change, but also desertification and loss of biodiversity are strongly affecting soils globally, making the “Soil Atlas of Latin America” relevant to a much larger community of stakeholders involved in the implementation of the three “Rio-Conventions” and allowing possible synergies among international multilateral agreements towards global soil protection to be explored.JRC.H.5-Land Resources Managemen

GlobalSoilMap: Toward a Fine-Resolution Global Grid of Soil Properties

Soil scientists are being challenged to provide assessments of soil condition from local through to global scales. A particular issue is the need for estimates of the stores and fluxes in soils of water, carbon, nutrients, and solutes. This review outlines progress in the development and testing of GlobalSoilMap—a digital soil map that aims to provide a fineresolution global grid of soil functional properties with estimates of their associated uncertainties. A range of methods can be used to generate the fine-resolution spatial estimates depending on the availability of existing soil surveys, environmental data, and point observations. The system has an explicit geometry for estimating point and block estimates of soil properties continuously down the soil profile. This geometry is necessary to ensure mass balance when stores and fluxes are computed. It also overcomes some limitations with existing systems for characterizing soil variation with depth. GlobalSoilMap has been designed to enable delivery of soil data via Web services. This review provides an overview of the system's technical specifications including the minimum data set. Examples from contrasting countries and environments are then presented to demonstrate the robustness of the technical specifications. GlobalSoilMap provides the means for supplying soil information in a format and resolution compatible with other fundamental data sets from remote sensing, terrain analysis, and other systems for mapping, monitoring, and forecasting biophysical processes. The initial research phase of the core project is nearing completion and attention is now shifting toward establishing the institutional and governance arrangements necessary to complete a full global coverage and maintaining the operational version of the GlobalSoilMap. This will be a grand and rewarding challenge for the soil science profession in the coming years.JRC.H.5-Land Resources Managemen

World’s soils are under threat

The Intergovernmental Technical Panel on Soils has completed the first State of the World’s Soil Resources report. Globally soil erosion was identified as the gravest threat, leading to deteriorating water quality in developed regions and to lowering of crop yields in many developing regions. We need to increase nitrogen and phosphorus fertilizer use in infertile tropical and semi-tropical soils – the regions where the most food insecure among us are found – while reducing global use of these products overall. Stores of soil organic carbon are critical in the global carbon balance, and national governments must set specific targets to stabilize or ideally increase soil organic carbon stores. Finally the quality of soil information available for policy formulation must be improved – the regional assessments in the SWSR report frequently base their evaluations on studies from the 1990s based on observations made in the 1980s or earlier.JRC.H.5-Land Resources Managemen

International Nosocomial Infection Control Consortiu (INICC) report, data summary of 43 countries for 2007-2012. Device-associated module

We report the results of an International Nosocomial Infection Control Consortium (INICC) surveillance study from January 2007-December 2012 in 503 intensive care units (ICUs) in Latin America, Asia, Africa, and Europe. During the 6-year study using the Centers for Disease Control and Prevention's (CDC) U.S. National Healthcare Safety Network (NHSN) definitions for device-associated health care–associated infection (DA-HAI), we collected prospective data from 605,310 patients hospitalized in the INICC's ICUs for an aggregate of 3,338,396 days. Although device utilization in the INICC's ICUs was similar to that reported from ICUs in the U.S. in the CDC's NHSN, rates of device-associated nosocomial infection were higher in the ICUs of the INICC hospitals: the pooled rate of central line–associated bloodstream infection in the INICC's ICUs, 4.9 per 1,000 central line days, is nearly 5-fold higher than the 0.9 per 1,000 central line days reported from comparable U.S. ICUs. The overall rate of ventilator-associated pneumonia was also higher (16.8 vs 1.1 per 1,000 ventilator days) as was the rate of catheter-associated urinary tract infection (5.5 vs 1.3 per 1,000 catheter days). Frequencies of resistance of Pseudomonas isolates to amikacin (42.8% vs 10%) and imipenem (42.4% vs 26.1%) and Klebsiella pneumoniae isolates to ceftazidime (71.2% vs 28.8%) and imipenem (19.6% vs 12.8%) were also higher in the INICC's ICUs compared with the ICUs of the CDC's NHSN