Computational Identification of Novel MicroRNAs and Their Targets in Vigna unguiculata

MicroRNAs (miRNAs) are a class of endogenous, noncoding, short RNAs directly involved in regulating gene expression at the posttranscriptional level. High conservation of miRNAs in plant provides the foundation for identification of new miRNAs in other plant species through homology alignment. Here, previous known plant miRNAs were BLASTed against the Expressed Sequence Tag (EST) and Genomic Survey Sequence (GSS) databases of Vigna unguiculata, and according to a series of filtering criteria, a total of 47 miRNAs belonging to 13 miRNA families were identified, and 30 potential target genes of them were subsequently predicted, most of which seemed to encode transcription factors or enzymes participating in regulation of development, growth, metabolism, and other physiological processes. Overall, our findings lay the foundation for further researches of miRNAs function in Vigna unguiculata

A survey of face detection, extraction and recognition

The goal of this paper is to present a critical survey of existing literatures on human face recognition over the last 4-5 years. Interest and research activities in face recognition have increased significantly over the past few years, especially after the American airliner tragedy on September 11 in 2001. While this growth largely is driven by growing application demands, such as static matching of controlled photographs as in mug shots matching, credit card verification to surveillance video images, identification for law enforcement and authentication for banking and security system access, advances in signal analysis techniques, such as wavelets and neural networks, are also important catalysts. As the number of proposed techniques increases, survey and evaluation becomes important

The upstream sequence of the phycocyanin \u3b2 subunit gene from Arthrospira platensis regulates expression of gfp gene in response to light intensity

In cyanobacteria, few details are known of the mechanisms through which the expression of the light-harvesting pigment c-phycocyanin is regulated. In the present study, a 419 bp upstream sequence of the phycocyanin \u3b2 subunit (cpcB) gene from Arthrospira platensis FACHB341 was fused with green fluorescent protein (gfp) gene, and a heterologous reporting system was built up to investigate the influence of light intensity on the expression of gfp gene, and the regulation function of different region of the upstream sequence of cpcB gene. Results showed that the upstream sequence of cpcB gene could drive the expression of gfp gene in Synechococcus sp. strain PCC7942, and the expression was influenced by light intensity, the lower the light intensity, the higher the gfp level. Deletion analysis revealed that a light-responsive element was located in the region -276 to-218, a promoter sequence was in the region -85 to -1, and two positive cis elements were in the -419 to -276 and the -218 to -130 regions, respectively

The Influence of Carrier's Attitude and the Position Reduction in Multibeam Echosounding and Airborne Laser Depth Sounding

Having finished the development of the multibeam echosounding system, China is making a great effort to develop an airborne laser depth sounding system. According to the principle of the two kinds of sounding system mentioned above, a series of position reduction formulas and their error equations are developed in this paper. The dynamic effect of marine sounding, i.e., the influence of carrier's attitude, is taken into full account in developing the equations. Finally, the real parameters of the two sounding systems developed by China are used to calculate the position reductions and their accuracies at different heading angles. The results show that the accuracies of depths and positions in multibeam echosounding and airborne laser depth sounding are dependent not only on their own sensors but also on the additional sensors

Assessment of the Rural Area Aid in the Czech Republic at the Regional Level

Import 22/07/2015Volba tématu a zpracování diplomové práce vycházelo z požadavků Agentury pro regionální rozvoj, a.s., v Ostravě na její praktické využití. Cílem diplomové práce je komparace a zhodnocení finanční podpory rozvoje venkovského prostoru na úrovni krajů České republiky v období 2007-2014. Práce se zaměřuje na analýzu podpory rozvoje venkova plynoucí z národních a evropských dotačních zdrojů, a to především z Programu rozvoje venkova ČR 2007-2013. Součástí analýzy je také zhodnocení čerpání dotačních prostředků na podporu rozvoje venkovského prostoru v Moravskoslezském kraji. Z provedené analýzy vyplývá, že na rozvoj venkovského prostoru více finančních prostředků bylo vynaložených z evropských než z národních zdrojů. Nejvíce žádostí o největším finančním objemu bylo schválených z Programu rozvoje venkova České republiky 2007-2013. Z provedené analýzy komparace krajů vyplývá, že mezi nejlepší kraje v čerpání dotačních prostředků určené na venkovský prostor patřil kraj Středočeský, Vysočina a Jihočeský. Moravskoslezský kraj se umístil na 9. místě. Z hlediska dotace na počet obcí, patřil mezi nejlepší kraje Karlovarský, Moravskoslezský a Jihočeský kraj. Dle ukazatele dotace na počet obyvatel, kraj Vysočina, Jihočeský a Karlovarský kraj. Moravskoslezský kraj se umístil na 8. místě.The theme and the processing of the thesis were based on the requirements of the Regional Development Agency in Ostrava to its practical use. The goal of this diploma thesis is a comparison and an evaluation of the financial support for the development of the rural area in the Czech Republic at the regional level in 2007-2014. This thesis is focused on an analysis or a rural development aid resulting from national and European funding sources, particularly from the Rural Development Programme of the Czech Republic 2007-2013. The study also includes an assesment of the utilization of grant funds to support the development of rural areas in the Moravian-Silesian Region. The analysis indicates that more financial sources were expended on the development of rural areas from the European funds than from the national ones. Most applications for the largest financial volume were approved by the Rural Development Programme of the Czech Republic 2007-2013. Conducted analysis of the comparison of the regions indicates that the Central Bohemia Region, the Vysocina Region and the South Bohemia Region belong to the best regions in the grants drawing. Moravian-Silesian Region was ranked 9th. In terms of the number of grants to municipalities, the best regions were the Karlovy Vary Region, the Moravian-Silesian Region and the South Bohemia Region. According to the indicator of the subsidies to the population, the Vysocina Region, the South Bohemia Region and the Karlovy Vary Region were the best. Moravian-Silesian Region was ranked 8th.120 - Katedra evropské integracevýborn

A network medicine approach to build a comprehensive atlas for the prognosis of human cancer

The Cancer Genome Atlas project has generated multi-dimensional and highly integrated genomic data from a large number of patient samples with detailed clinical records across many cancer types, but it remains unclear how to best integrate the massive amount of genomic data into clinical practice. We report here our methodology to build a multi-dimensional subnetwork atlas for cancer prognosis to better investigate the potential impact of multiple genetic and epigenetic (gene expression, copy number variation, microRNA expression and DNA methylation) changes on the molecular states of networks that in turn affects complex cancer survivorship. We uncover an average of 38 novel subnetworks in the protein-protein interaction network that correlate with prognosis across four prominent cancer types. The clinical utility of these subnetwork biomarkers was further evaluated by prognostic impact evaluation, functional enrichment analysis, drug target annotation, tumor stratification and independent validation. Some pathways including the dynactin, cohesion and pyruvate dehydrogenase-related subnetworks are identified as promising new targets for therapy in specific cancer types. In conclusion, this integrative analysis of existing protein interactome and cancer genomics data allows us to systematically dissect the molecular mechanisms that underlie unexpected outcomes for cancer, which could be used to better understand and predict clinical outcomes, optimize treatment and to provide new opportunities for developing therapeutics related to the subnetworks identified

NADPH oxidase 5 (NOX5)-induced reactive oxygen signaling modulates normoxic HIF-1α and p27

NADPH oxidase 5 (NOX5) generated reactive oxygen species (ROS) have been implicated in signaling cascades that regulate cancer cell proliferation. To evaluate and validate NOX5 expression in human tumors, we screened a broad range of tissue microarrays (TMAs), and report substantial overexpression of NOX5 in malignant melanoma and cancers of the prostate, breast, and ovary. In human UACC-257 melanoma cells that possesses high levels of functional endogenous NOX5, overexpression of NOX5 resulted in enhanced cell growth, increased numbers of BrdU positive cells, and increased γ-H2AX levels. Additionally, NOX5-overexpressing (stable and inducible) UACC-257 cells demonstrated increased normoxic HIF-1α expression and decreased p2

Genome-wide identification and analysis of heterotic loci in three maize hybrids

Heterosis, or hybrid vigour, is a predominant phenomenon in plant genetics, serving as the basis of crop hybrid breeding, but the causative loci and genes underlying heterosis remain unclear in many crops. Here, we present a large-scale genetic analysis using 5360 offsprings from three elite maize hybrids, which identifies 628 loci underlying 19 yield-related traits with relatively high mapping resolutions. Heterotic pattern investigations of the 628 loci show that numerous loci, mostly with complete–incomplete dominance (the major one) or overdominance effects (the secondary one) for heterozygous genotypes and nearly equal proportion of advantageous alleles from both parental lines, are the major causes of strong heterosis in these hybrids. Follow-up studies for 17 heterotic loci in an independent experiment using 2225 F2 individuals suggest most heterotic effects are roughly stable between environments with a small variation. Candidate gene analysis for one major heterotic locus (ub3) in maize implies that there may exist some common genes contributing to crop heterosis. These results provide a community resource for genetics studies in maize and new implications for heterosis in plants