In cyanobacteria, few details are known of the mechanisms through which
the expression of the light-harvesting pigment c-phycocyanin is
regulated. In the present study, a 419 bp upstream sequence of the
phycocyanin \u3b2 subunit (cpcB) gene from Arthrospira platensis
FACHB341 was fused with green fluorescent protein (gfp) gene, and a
heterologous reporting system was built up to investigate the influence
of light intensity on the expression of gfp gene, and the regulation
function of different region of the upstream sequence of cpcB gene.
Results showed that the upstream sequence of cpcB gene could drive the
expression of gfp gene in Synechococcus sp. strain PCC7942, and the
expression was influenced by light intensity, the lower the light
intensity, the higher the gfp level. Deletion analysis revealed that a
light-responsive element was located in the region -276 to-218, a
promoter sequence was in the region -85 to -1, and two positive cis
elements were in the -419 to -276 and the -218 to -130 regions,
respectively