Supplement 1. A phylogenetic tree and key to species codes for tree species occurring within the 50-ha Forest Dynamics Plot (FDP) on Barro Colorado Island, Panama.

<h2>File List</h2><blockquote> <p><a href="FDP_phylogeny.new.txt">FDP_phylogeny.new.txt</a><br> <a href="FDP_phylogeny_species_code_lookup_table.csv">FDP_phylogeny_species_code_lookup_table.csv</a></p> <p> </p></blockquote><h2>Description</h2><blockquote> <p>The file FDP_phylogeny.new.txt is an ASCII text file containing the phylogenetic tree used in this study in parenthetical Newick format. The phylogenetic tree was generated using Phylomatic tree version R20031202 software (Webb and Donoghue 2005). Internal nodes of the tree were labeled to allow node age estimation using the BLADJ module of Phylocom version 3.19 software (Webb et al. 2004). Named internal nodes were dated based on clade age estimates provided by Wikström et al. (2001). Units of the branch lengths in the phylogeny are millions of years. <br> <br> Species codes used in the phylogenetic tree file can be resolved to full species names using the lookup table presented in the comma-separated ASCII text file FDP_phylogeny_species_code_lookup_table.csv.<br> Column definitions:<br> 1 - Code - species code used in FDP_phylogeny.new.txt<br> 2 - SpeciesName - Species name corresponding to each species code.<br> </p> </blockquote

Supplementary Material: Phylogeny of all seeded plant families

Climate change is driving rapid and accelerating shifts in range limits, both poleward expansions and equatorward contractions.&nbsp; However, many species are falling behind the pace of change in their dispersal into newly suitable habitats and now show “climate debts”, lags between predicted and observed range expansions under changing climates. Failure to track changing climates may be due to interspecific interactions such as particular food availability for specialists, abiotic barriers such as mountain ranges, or intrinsic traits such as dispersal limitation. A trait-based analysis of climate change performance would help identify causes of climate debt. To understand the correlates of climate debt within a large clade of organisms we use historical and modern observations of butterflies from western Canada as a case study to construct and project individual climate-based environmental niche models. By comparing projected distributions based on historical records to observed modern distributions we are able to construct estimates of climate debt and evaluate the effect of dispersal ability, diet breadth and a proxy for range size on these species\u27 measured climate debt. High levels of climate debt are accumulating within the butterflies of Western Canada, independently of dispersal ability, diet breadth and phylogeny. Range size emerges as the only variable that significantly reduces climate debt, suggesting that more narrowly-ranged species may be at risk of being squeezed out by both a reduction of suitable habitat in their current range and the failure to colonize newly available habitat. These findings underscore the need to investigate potential landscape-level determinants of climate debt that may be limiting range expansions in this group

Plant compartment and biogeography affect microbiome composition in cultivated and native Agave species.

Desert plants are hypothesized to survive the environmental stress inherent to these regions in part thanks to symbioses with microorganisms, and yet these microbial species, the communities they form, and the forces that influence them are poorly understood. Here we report the first comprehensive investigation of the microbial communities associated with species of Agave, which are native to semiarid and arid regions of Central and North America and are emerging as biofuel feedstocks. We examined prokaryotic and fungal communities in the rhizosphere, phyllosphere, leaf and root endosphere, as well as proximal and distal soil samples from cultivated and native agaves, through Illumina amplicon sequencing. Phylogenetic profiling revealed that the composition of prokaryotic communities was primarily determined by the plant compartment, whereas the composition of fungal communities was mainly influenced by the biogeography of the host species. Cultivated A.&nbsp;tequilana exhibited lower levels of prokaryotic diversity compared with native agaves, although no differences in microbial diversity were found in the endosphere. Agaves shared core prokaryotic and fungal taxa known to promote plant growth and confer tolerance to abiotic stress, which suggests common principles underpinning Agave-microbe interactions