Effects of propofol combined with remifentanil on hemodynamics and stress response in children undergoing surgery for oral cancers, tonsil and adenoid surgery

727-733The anesthetic medication to sedate a child during general anesthesia (GA) for oral cancer, adenoidectomy or tonsillectomy is associated with operative complications such as hemodynamic instability and long postoperative recovery period. The current advancement enables combination of different anesthetic medications to decrease operative or postoperative complications associated with GA. In this study assessed the effects of propofol combined with remifentanil on hemodynamics and stress response in children undergoing oral cancer, tonsil and adenoid surgery. Propofol combined with remifentanil is beneficial to anesthesia for children undergoing oral cancer tonsil and adenoid surgery, manifested as stable hemodynamics, rapid recovery, low inflammatory and stress responses, and mild adverse reactions. A total of 106 eligible children treated from May 2017 to December 2019 were randomly divided into observation and control groups (n=53). Observation group was anesthetized by propofol plus remifentanil, while control group was anesthetized by propofol plus esketamine. Mean arterial pressure (MAP), heart rate (HR), serum C-reactive protein (CRP), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), epinephrine (E), cortisol (Cor), CD3+, CD4+ helper and CD8+ inhibitory T lymphocytes, and CD4+/CD8+were compared before anesthesia induction (T1), immediately after intubation (T2), at the beginning of operation (T3), at the end of operation (T4) and 5 min after extubation (T5). Time of anesthetic recovery and adverse reactions after extubation were observed. MAP and HR significantly rose at T2 compared with those at T1. After maintenance of anesthesia, MAP and HR were significantly lower in observation group than those in control group. Serum CRP, IL-6 and TNF-α levels rose with time. E and Cor levels rose from T1 to T4 and declined at T5, with significant differences at each time point. CRP, IL-6, TNF-α, E and Cor levels were lower in observation group from T3 to T5. At T4 and T5, CD3+, CD4+levels and CD4+/CD8+ declined, whileCD8+level rose compared with those at other three time points. Time of recovery of autonomous respiration and limbs and duration from anesthetic withdrawal to extubation were significantly shorter in observation group. Observation group had lower incidence rate of dysphoria during recovery

Effects of propofol combined with remifentanil on hemodynamics and stress response in children undergoing surgery for oral cancers, tonsil and adenoid surgery

The anesthetic medication to sedate a child during general anesthesia (GA) for oral cancer, adenoidectomy or tonsillectomy is associated with operative complications such as hemodynamic instability and long postoperative recovery period. The current advancement enables combination of different anesthetic medications to decrease operative or postoperative complications associated with GA. In this study assessed the effects of propofol combined with remifentanil on hemodynamics and stress response in children undergoing oral cancer, tonsil and adenoid surgery. Propofol combined with remifentanil is beneficial to anesthesia for children undergoing oral cancer tonsil and adenoid surgery, manifested as stable hemodynamics, rapid recovery, low inflammatory and stress responses, and mild adverse reactions. A total of 106 eligible children treated from May 2017 to December 2019 were randomly divided into observation and control groups (n=53). Observation group was anesthetized by propofol plus remifentanil, while control group was anesthetized by propofol plus esketamine. Mean arterial pressure (MAP), heart rate (HR), serum C-reactive protein (CRP), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), epinephrine (E), cortisol (Cor), CD3+, CD4+ helper and CD8+ inhibitory T lymphocytes, and CD4+/CD8+were compared before anesthesia induction (T1), immediately after intubation (T2), at the beginning of operation (T3), at the end of operation (T4) and 5 min after extubation (T5). Time of anesthetic recovery and adverse reactions after extubation were observed. MAP and HR significantly rose at T2 compared with those at T1. After maintenance of anesthesia, MAP and HR were significantly lower in observation group than those in control group. Serum CRP, IL-6 and TNF-α levels rose with time. E and Cor levels rose from T1 to T4 and declined at T5, with significant differences at each time point. CRP, IL-6, TNF-α, E and Cor levels were lower in observation group from T3 to T5. At T4 and T5, CD3+, CD4+levels and CD4+/CD8+ declined, whileCD8+level rose compared with those at other three time points. Time of recovery of autonomous respiration and limbs and duration from anesthetic withdrawal to extubation were significantly shorter in observation group. Observation group had lower incidence rate of dysphoria during recovery

Heterologous Expression of a Novel Zoysia japonica C2H2 Zinc Finger Gene, ZjZFN1, Improved Salt Tolerance in Arabidopsis

Growing evidence indicates that some grass species are more tolerant to various abiotic and biotic stresses than many crops. Zinc finger proteins play important roles in plant abiotic and biotic stresses. Although genes coding for these proteins have been cloned and identified in various plants, their function and underlying transcriptional mechanisms in the halophyte Zoysia japonica are barely known. In the present study, ZjZFN1 was isolated from Z. japonica using RACE method. Quantitative real time PCR results revealed that the expression of ZjZFN1 was much higher in leaf than in root and stem tissues, and induced by salt, cold or ABA treatment. The subcellular localization assay demonstrated that ZjZFN1 was localized to the nucleus. Expression of the ZjZFN1 in Arabidopsis thaliana improved seed germination and enhanced plant adaption to salinity stress with improved percentage of green cotyledons and growth status under salinity stress. Physiological and transcriptional analyses suggested that ZjZFN1 might, at least in part, influence reactive oxygen species accumulation and regulate the transcription of salinity responsive genes. Furthermore, RNA-sequencing analysis of ZjZFN1-overexpressing plants revealed that ZjZFN1 may serve as a transcriptional activator in the regulation of stress responsive pathways, including phenylalanine metabolism, α-linolenic acid metabolism and phenylpropanoid biosynthesis pathways. Taken together, these results provide evidence that ZjZFN1 is a potential key player in plants’ tolerance to salt stress, and it could be a valuable gene in Z. japonica breeding projects

Chloroplast genomes of four Carex species: Long repetitive sequences trigger dramatic changes in chloroplast genome structure

The chloroplast genomes of angiosperms usually have a stable circular quadripartite structure that exhibits high consistency in genome size and gene order. As one of the most diverse genera of angiosperms, Carex is of great value for the study of evolutionary relationships and speciation within its genus, but the study of the structure of its chloroplast genome is limited due to its highly expanded and restructured genome with a large number of repeats. In this study, we provided a more detailed account of the chloroplast genomes of Carex using a hybrid assembly of second- and third-generation sequencing and examined structural variation within this genus. The study revealed that chloroplast genomes of four Carex species are significantly longer than that of most angiosperms and are characterized by high sequence rearrangement rates, low GC content and gene density, and increased repetitive sequences. The location of chloroplast genome structural variation in the species of Carex studied is closely related to the positions of long repeat sequences; this genus provides a typical example of chloroplast structural variation and expansion caused by long repeats. Phylogenetic relationships constructed based on the chloroplast protein-coding genes support the latest taxonomic system of Carex, while revealing that structural variation in the chloroplast genome of Carex may have some phylogenetic significance. Moreover, this study demonstrated a hybrid assembly approach based on long and short reads to analyze complex chloroplast genome assembly and also provided an important reference for the analysis of structural rearrangements of chloroplast genomes in other taxa

Chromosome-level reference genome assembly provides insights into the evolution of Pennisetum alopecuroides

Pennisetum alopecuroides is an important forage grass resource, which plays a vital role in ecological environment improvement. Therefore, the acquisition of P. alopecuroides genome resources is conducive to the study of the adaptability of Pennisetum species in ecological remediation and forage breeding development. Here we assembled a P. alopecuroides cv. 'Liqiu' genome at the chromosome level with a size of approximately 845.71 Mb, contig N50 of 84.83Mb, and genome integrity of 99.13% as assessed by CEGMA. A total of 833.41-Mb sequences were mounted on nine chromosomes by Hi-C technology. In total, 60.66% of the repetitive sequences and 34,312 genes were predicted. The genomic evolution analysis showed that P. alopecuroides cv. 'Liqiu' was isolated from Setaria 7.53–13.80 million years ago and from Cenchrus 5.33–8.99 million years ago, respectively. The whole-genome event analysis showed that P. alopecuroides cv. 'Liqiu' underwent two whole-genome duplication (WGD) events in the evolution process, and the duplication events occurred at a similar time to that of Oryza sativa and Setaria viridis. The completion of the genome sequencing of P. alopecuroides cv. 'Liqiu' provides data support for mining high-quality genetic resources of P. alopecuroides and provides a theoretical basis for the origin and evolutionary characteristics of Pennisetum

Relative Unidirectional Translation in an Artificial Molecular Assembly Fueled by Light

Motor molecules present in nature convert energy inputs, such as a chemical fuel or incident photons of light, into directed motion and force biochemical systems away from thermal equilibrium. The ability not only to control relative movements of components in molecules but also to drive their components preferentially in one direction relative to each other using versatile stimuli is one of the keys to future technological applications. Herein, we describe a wholly synthetic small-molecule system that, under the influence of chemical reagents, electrical potential, or visible light, undergoes unidirectional relative translational motion. Altering the redox state of a cyclobis(paraquat-p-phenylene) ring simultaneously (i) inverts the relative heights of kinetic barriers presented by the two termini—one a neutral 2-isopropylphenyl group and the other a positively charged 3,5-dimethylpyridinium unit—of a constitutionally asymmetric dumbbell, which can impair the threading/dethreading of a [2]pseudorotaxane, and (ii) controls the ring’s affinity for a 1,5-dioxynaphthalene binding site located in the dumbbell’s central core. The formation and subsequent dissociation of the [2]pseudorotaxane by passage of the ring over the neutral and positively charged termini of the dumbbell component in one, and only one, direction relatively defined has been demonstrated by (i) spectroscopic (1H NMR and UV/vis) means and cyclic voltammetry as well as with (ii) DFT calculations and by (iii) comparison with control compounds in the shape of constitutionally symmetrical [2]pseudorotaxanes, one with two positively charged ends and the other with two neutral ends. The operation of the system relies solely on reversible, yet stable, noncovalent bonding interactions. Moreover, in the presence of a photosensitizer, visible-light energy is the only fuel source that is needed to drive the unidirectional molecular translation, making it feasible to repeat the operation numerous times without the buildup of byproducts

Mechanical Bond-Induced Radical Stabilization

A homologous series of [2]­rotaxanes, in which cyclobis­(paraquat-<i>p</i>-phenylene) (CBPQT⁴⁺) serves as the ring component, while the dumbbell components all contain single 4,4′-bipyridinium (BIPY²⁺) units centrally located in the midst of oligomethylene chains of varying lengths, have been synthesized by taking advantage of radical templation and copper-free azide–alkyne 1,3-dipolar cycloadditions in the formation of their stoppers. Cyclic voltammetry, UV/vis spectroscopy, and mass spectrometry reveal that the BIPY^•+ radical cations in this series of [2]­rotaxanes are stabilized against oxidation, both electrochemically and by atmospheric oxygen. The enforced proximity between the BIPY²⁺ units in the ring and dumbbell components gives rise to enhanced Coulombic repulsion, destabilizing the ground-state co-conformations of the fully oxidized forms of these [2]­rotaxanes. The smallest [2]­rotaxane, with only three methylene groups on each side of its dumbbell component, is found to exist under ambient conditions in a monoradical state, a situation which does not persist in acetonitrile solution, at least in the case of its longer analogues. ¹H NMR spectroscopy reveals that the activation energy barriers to the shuttling of the CBPQT⁴⁺ rings over the BIPY²⁺ units in the dumbbells increase linearly with increasing oligomethylene chain lengths across the series of [2]­rotaxanes. These findings provide a new way of producing highly stabilized BIPY^•+ radical cations and open up more opportunities to use stable organic radicals as building blocks for the construction of paramagnetic materials and conductive molecular electronic devices