Off-Axis Nulling Transfer Function Measurement: A First Assessment

We want to study a polychromatic inverse problem method with nulling interferometers to obtain information on the structures of the exozodiacal light. For this reason, during the first semester of 2013, thanks to the support of the consortium PERSEE, we launched a campaign of laboratory measurements with the nulling interferometric test bench PERSEE, operating with 9 spectral channels between J and K bands. Our objective is to characterise the transfer function, i.e. the map of the null as a function of wavelength for an off-axis source, the null being optimised on the central source or on the source photocenter. We were able to reach on-axis null depths better than 10(exp 4). This work is part of a broader project aiming at creating a simulator of a nulling interferometer in which typical noises of a real instrument are introduced. We present here our first results

Autoacetylation of the Ralstonia solanacearum Effector PopP2 Targets a Lysine Residue Essential for RRS1-R-Mediated Immunity in Arabidopsis

Type III effector proteins from bacterial pathogens manipulate components of host immunity to suppress defence responses and promote pathogen development. In plants, host proteins targeted by some effectors called avirulence proteins are surveyed by plant disease resistance proteins referred to as “guards”. The Ralstonia solanacearum effector protein PopP2 triggers immunity in Arabidopsis following its perception by the RRS1-R resistance protein. Here, we show that PopP2 interacts with RRS1-R in the nucleus of living plant cells. PopP2 belongs to the YopJ-like family of cysteine proteases, which share a conserved catalytic triad that includes a highly conserved cysteine residue. The catalytic cysteine mutant PopP2-C321A is impaired in its avirulence activity although it is still able to interact with RRS1-R. In addition, PopP2 prevents proteasomal degradation of RRS1-R, independent of the presence of an integral PopP2 catalytic core. A liquid chromatography/tandem mass spectrometry analysis showed that PopP2 displays acetyl-transferase activity leading to its autoacetylation on a particular lysine residue, which is well conserved among all members of the YopJ family. These data suggest that this lysine residue may correspond to a key binding site for acetyl-coenzyme A required for protein activity. Indeed, mutation of this lysine in PopP2 abolishes RRS1-R-mediated immunity. In agreement with the guard hypothesis, our results favour the idea that activation of the plant immune response by RRS1-R depends not only on the physical interaction between the two proteins but also on its perception of PopP2 enzymatic activity

The SuperCam Instrument Suite on the Mars 2020 Rover: Science Objectives and Mast-Unit Description

On the NASA 2020 rover mission to Jezero crater, the remote determination of the texture, mineralogy and chemistry of rocks is essential to quickly and thoroughly characterize an area and to optimize the selection of samples for return to Earth. As part of the Perseverance payload, SuperCam is a suite of five techniques that provide critical and complementary observations via Laser-Induced Breakdown Spectroscopy (LIBS), Time-Resolved Raman and Luminescence (TRR/L), visible and near-infrared spectroscopy (VISIR), high-resolution color imaging (RMI), and acoustic recording (MIC). SuperCam operates at remote distances, primarily 2-7 m, while providing data at sub-mm to mm scales. We report on SuperCam's science objectives in the context of the Mars 2020 mission goals and ways the different techniques can address these questions. The instrument is made up of three separate subsystems: the Mast Unit is designed and built in France; the Body Unit is provided by the United States; the calibration target holder is contributed by Spain, and the targets themselves by the entire science team. This publication focuses on the design, development, and tests of the Mast Unit; companion papers describe the other units. The goal of this work is to provide an understanding of the technical choices made, the constraints that were imposed, and ultimately the validated performance of the flight model as it leaves Earth, and it will serve as the foundation for Mars operations and future processing of the data.In France was provided by the Centre National d'Etudes Spatiales (CNES). Human resources were provided in part by the Centre National de la Recherche Scientifique (CNRS) and universities. Funding was provided in the US by NASA's Mars Exploration Program. Some funding of data analyses at Los Alamos National Laboratory (LANL) was provided by laboratory-directed research and development funds

3D GEOVISUALIZATION & STYLIZATION TO MANAGE COMPREHENSIVE AND PARTICIPATIVE LOCAL URBAN PLANS

3D geo-visualization is more and more used and appreciated to support public participation, and is generally used to present predesigned planned projects. Nevertheless, other participatory processes may benefit from such technology such as the elaboration of urban planning documents. In this article, we present one of the objectives of the PLU++ project: the design of a 3D geo-visualization system that eases the participation concerning local urban plans. Through a pluridisciplinary approach, it aims at covering the different aspects of such a system: the simulation of built configurations to represent regulation information, the efficient stylization of these objects to make people understand their meanings and the interaction between 3D simulation and stylization. The system aims at being adaptive according to the participation context and to the dynamic of the participation. It will offer the possibility to modify simulation results and the rendering styles of the 3D representations to support participation. The proposed 3D rendering styles will be used in a set of practical experiments in order to test and validate some hypothesis from past researches of the project members about 3D simulation, 3D semiotics and knowledge about uses

Compositional maps of 67P/CG by Rosetta/VIRTIS-M

The Visible InfraRed Thermal Imaging Spectrometer, VIRTIS onboard ESA’s Rosetta mission has completed two extensive mapping campaigns of the 67P/Churyumov-Gerasimenko comet nucleus in August-September 2014. After calibration and geometrical projection on nucleus shape model, VIRTIS data were processed to retrieve the distribution of several spectral indicators (spectral slopes, organic material band depths) from which compositional maps are built. Apart from being appropriate to map surface composition, those indicators allow us to correlate them with local morphological features (patches, boulders, scarps, terraces, active areas) and thermal properties

Compositional maps of 67P/CG nucleus after perihelion passage by VIRTIS-M aboard Rosetta

Comet 67P/Churyumov-Gerasimenko undergoes periherm transit at 1.2 AU in August 2015. During this passage the illumination conditions above the south hemisphere of the nucleus rapidly improve becoming optimal for the retrieval of the surface properties by VIRTIS-M onboard Rosetta. A similar mapping of the surface at about 12.5 m/pixel and at solar phases below 40 deg has been already performed during the Philae prelanding phase (August-September 2014, heliocentric distance 3.6 AU) allowing us to build compositional maps of the entire north hemisphere and equatorial regions down to latitudes -50 deg on a limited part of the Hapi, or the comet’s neck, region. One year after this first mapping campaign the illumination geometry becomes favorable to complete the coverage above the South polar region. Since comet’s activity is rapidly increasing and the Rosetta spacecraft cannot orbit on low trajectories like during the prelanding phase, the south hemisphere maps shall be reasonably observed by VIRTIS-M with a spatial resolution of about 25 m/pixel. Global scale data have shown that the nucleus’ double-lobe surface is characterized by morphologically different units uniformly covered by a very dark, low-albedo, dehydrated organic-rich material. Compositional properties across the different regions of the nucleus are mapped by measuring visible and infrared spectral slopes, calculated on the best linear fit to the reflectance spectra between 0.5-0.8 μm and 1-2.5 μm, respectively. As pre-landing data have clearly shown, spectral slopes are highly diagnostic to identify active areas, like in the Hapi area, and exposed water ice deposits where the spectra appear less red. As heliocentric distance decreases and diurnal temperatures increase, the 3-5 μm spectral range becomes affected by thermal emission from the surface. This emission is overlapping with the 3 μm feature previously observed by VIRTIS during the pre-landing period making more difficult to retrieve the distribution of the organic material. A summary of the spectral characteristics observed on the south hemisphere region during the perihelion passage is given. Activity-driven spectral changes observed before and after perihelion passage on some specific areas of the surface are discussed

First observations of H2O and CO2vapor in comet 67P/Churyumov-Gerasimenko made by VIRTIS onboard Rosetta

Context. Outgassing from cometary nuclei involves complex surface and subsurface processes that need to be understood to investigate the composition of cometary ices from coma observations. Aims. We investigate the production of water, carbon dioxide, and carbon monoxide from the nucleus of comet 67P/Churyumov-Gerasimenko (67P). These species have different volatility and are key species of cometary ices. Methods. Using the high spectral-resolution channel of the Visible InfraRed Thermal Imaging Spectrometer (VIRTIS-H), we observed the ν3 vibrational bands of H2O and CO2 at 2.67 and 4.27 μm, respectively, from 24 November 2014 to 24 January 2015, when comet 67P was between 2.91 and 2.47 AU from the Sun. Observations were undertaken in limb-viewing geometry at distances from the surface of 0 to 1.5 km and with various line-of-sight (LOS) orientations in the body-fixed frame. A geometry tool was used to characterize the position of the LOS with respect to geomorphologic regions and the illumination properties of these regions. Results. The water production of 67P did not increase much from 2.9 to 2.5 AU. High water column densities are observed for LOS above the neck regions, suggesting they are the most productive in water vapor. While water production is weak in regions with low solar illumination, CO2 is outgassing from both illuminated and non-illuminated regions, which indicates that CO2 sublimates at a depth that is below the diurnal skin depth. The CO2/H2O column density ratio varies from 2 to 60%. For regions that are in sunlight, mean values between 2 and 7% are measured. The lower bound value is likely representative of the CO2/H2O production rate ratio from the neck regions. For carbon monoxide, we derive column density ratios CO/H2O < 1.9% and CO/CO2< 80%. An illumination-driven model, with a uniformly active surface releasing water at a mean rate of 8 × 1025 s-1, provides an overall agreement to VIRTIS-H data, although some mismatches show local surface inhomogeneities in water production. Rotational temperatures of 90–100 K are derived from H2O and CO2 averaged spectra

Seasonal exposure of carbon dioxide ice on the nucleus of comet 67P/Churyumov-Gerasimenko

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The organic-rich surface of comet 67P/Churyumov-Gerasimenko as seen by VIRTIS/Rosetta

The VIRTIS (Visible, Infrared and Thermal Imaging Spectrometer) instrument on board the Rosetta spacecraft has provided evidence of carbon-bearing compounds on the nucleus of the comet 67P/Churyumov-Gerasimenko. The very low reflectance of the nucleus (normal albedo of 0.060 −/+ 0.003 at 0.55 micrometers), the spectral slopes in visible and infrared ranges (5 to 25 and 1.5 to 5% k/Å), and the broad absorption feature in the 2.9-to-3.6 micrometer range present across the entire illuminated surface are compatible with opaque minerals associated with nonvolatile organic macromolecular materials: a complex mixture of various types of carbon-hydrogen and/or oxygen-hydrogen chemical groups, with little contribution of nitrogen-hydrogen groups. In active areas, the changes in spectral slope and absorption feature width may suggest small amounts of water-ice. However, no ice-rich patches are observed, indicating a generally dehydrated nature for the surface currently illuminated by the Sun