Nucleon form factors and a nonpointlike diquark

Nucleon form factors are calculated on q^2 in [0,3] GeV^2 using an Ansatz for the nucleon's Fadde'ev amplitude motivated by quark-diquark solutions of the relativistic Fadde'ev equation. Only the scalar diquark is retained, and it and the quark are confined. A good description of the data requires a nonpointlike diquark correlation with an electromagnetic radius of 0.8 r_pi. The composite, nonpointlike nature of the diquark is crucial. It provides for diquark-breakup terms that are of greater importance than the diquark photon absorption contribution.Comment: 5 pages, REVTEX, epsfig, 3 figure

Bound q2qˉ2q^2\bar q^2 states in a constituent quark model

We consider the existence of bound systems consisting of two quarks and two antiquarks ($q^2\bar q^2$) within the framework of a constituent quark model. The underlying quark dynamics is described by a linear confinement potential and an effective $q^2\bar q^2$ interaction which has its origin in instanton effects of QCD. We calculate the spectra and examine the internal structure of the states found.Comment: 11 pages, needs epsf.st

Electromagnetic form factors of the nucleon in a covariant diquark model

We present a simple covariant constituent diquark-quark model for the nucleon. The nucleon is assumed to be composed of a scalar diquark and a quark which interact via a quark exchange. Starting from the Bethe-Salpeter equation, the instantaneous approximation leads to a diquark-quark Salpeter equation. In the Mandelstam formalism, the electromagnetic form factors of the nucleon are calculated for momentum transfers up to q^2 = - 3 \; (\mbox{GeV/c})^2. A remarkable description of the experimental data is obtained. Especially, the model gives nearly the right values for the proton and (negative) neutron charge radii, and a qualitative description of the magnetic form factors.Comment: 17 pages, revtex, 8 figures in additional fil

A transient homotypic interaction model for the influenza A virus NS1 protein effector domain

Influenza A virus NS1 protein is a multifunctional virulence factor consisting of an RNA binding domain (RBD), a short linker, an effector domain (ED), and a C-terminal 'tail'. Although poorly understood, NS1 multimerization may autoregulate its actions. While RBD dimerization seems functionally conserved, two possible apo ED dimers have been proposed (helix-helix and strand-strand). Here, we analyze all available RBD, ED, and full-length NS1 structures, including four novel crystal structures obtained using EDs from divergent human and avian viruses, as well as two forms of a monomeric ED mutant. The data reveal the helix-helix interface as the only strictly conserved ED homodimeric contact. Furthermore, a mutant NS1 unable to form the helix-helix dimer is compromised in its ability to bind dsRNA efficiently, implying that ED multimerization influences RBD activity. Our bioinformatical work also suggests that the helix-helix interface is variable and transient, thereby allowing two ED monomers to twist relative to one another and possibly separate. In this regard, we found a mAb that recognizes NS1 via a residue completely buried within the ED helix-helix interface, and which may help highlight potential different conformational populations of NS1 (putatively termed 'helix-closed' and 'helix-open') in virus-infected cells. 'Helix-closed' conformations appear to enhance dsRNA binding, and 'helix-open' conformations allow otherwise inaccessible interactions with host factors. Our data support a new model of NS1 regulation in which the RBD remains dimeric throughout infection, while the ED switches between several quaternary states in order to expand its functional space. Such a concept may be applicable to other small multifunctional proteins

Cytotoxic effect of sodium hypochlorite (NaClO) in apical cells of onion roots (Allium cepa L.)

Páginas 97-104.El hipoclorito de sodio (NaClO), es un compuesto químico altamente tóxico y ampliamente utilizado por sus propiedades desinfectantes. Una de las especies vegetales más usadas para la detección de diferentes tipos de cambios genéticos causados por sustancias químicas es Allium cepa L. mediante la cual se hacen pruebas para la vigilancia del medio ambiente. El objetivo de esta investigación fue evaluar el efecto citotóxico del hipoclorito de sodio (NaClO) en células apicales de raíces de cebolla (Allium cepa L.). Se prepararon cinco soluciones de hipoclorito de sodio a diferentes concentraciones más una solución control con tres réplicas para cada tratamiento; se midió el crecimiento de las raíces durante 3 días, se cortaron los ápices sumergiéndose en ácido clorhídrico, posteriormente fueron teñidos con Aceto-Orceina y se observaron al microscopio; se calculó el índice mitótico (IM) y se observaron las anomalías celulares (cromosómicas). Se realizó un análisis de varianza (ANOVA) y prueba de rangos múltiples mediante el método de diferencia mínima significativa (LSD). Los resultados mostraron que no existe una diferencia estadísticamente significativa entre los tratamientos (P>0,05). La concentración donde se presentó un mayor IM fue de 1 ppm. Se evidenciaron las anomalías celulares: en anafase, evidenciando puentes entre cromosomas, hipercromasia, lesiones nucleares y rompimiento de cromosomas en anafase y metafase. Los resultados evidenciaron que Allium cepa es una especie que ofrece un modelo experimental para evaluar el efecto citotóxico, en este caso del hipoclorito de sodio, el cual generó anomalías celulares (cromosómicas) en todas las concentraciones.ABSTRACT: (NaClO) sodium hypochlorite is a highly toxic chemical compound widely used because of its disinfectant properties. One of the most commonly used plants to detect different types of genetic changes caused by chemical substances is Allium cepa L., in tests for environmental monitoring. The objective of this research was to evaluate the cytotoxic effect of sodium hypochlorite (NaClO) in apical cells of onion roots (Allium strain L.). Five sodium hypochlorite solutions were prepared at different concentrations, in addition to a control solution, with three replications for each treatment; for 3 days, the growth of the roots was measured; the apices were cut submerging them into hydrochloric acid, subsequently dyed with Aceto-Orcein and were observed under a microscope; the mitotic index (MI) was calculated and the cell anomalies were observed (chromosomal). An analysis of variance (ANOVA) and multiple range tests using the Least Significant Difference method (LSD) were performed. The results showed that there was not a statistically significant difference between the treatments (P0.05). The concentration with the highest MI was 1 ppm. The following cellular abnormalities were evidenced: bridge between chromosomes in anaphase, chromatin, nuclear injuries, chromosome breakage in anaphase and metaphase. The results showed that Allium cepa L. is a species that offers an experimental model to evaluate the cytotoxic effect, in this case sodium hypochlorite, which generated cellular abnormalities (chromosomal) in all concentrations.Bibliografía: página 104.Artículo revisado por pares

A hybrid approach to CMB lensing reconstruction on all-sky intensity maps

Based on realistic simulations, we propose an hybrid method to reconstruct the lensing potential power spectrum, directly on PLANCK-like CMB frequency maps. It implies using a large galactic mask and dealing with a strong inhomogeneous noise. For l < 100, we show that a full-sky inpainting method, already described in a previous work, still allows a minimal variance reconstruction, with a bias that must be accounted for by a Monte-Carlo method, but that does not couple to the deflection field. For l>100 we develop a method based on tiling the cut-sky with local 10x10 degrees overlapping tangent planes (referred to in the following as "patches"). It requires to solve various issues concerning their size/position, non-periodic boundaries and irregularly sampled data after the sphere-to-plane projection. We show how the leading noise term of the quadratic lensing estimator applied onto an apodized patch can still be taken directly from the data. To not loose spatial accuracy, we developed a tool that allows the fast determination of the complex Fourier series coefficients from a bi-dimensional irregularly sampled dataset, without performing an interpolation. We show that the multi-patch approach allows the lensing power spectrum reconstruction with a very small bias, thanks to avoiding the galactic mask and lowering the noise inhomogeneities, while still having almost a minimal variance. The data quality can be assessed at each stage and simple bi-dimensional spectra build, which allows the control of local systematic errors.Comment: A&A version. Mostly english correction

Sensorimotor Experience Influences Recovery of Forelimb Abilities but Not Tissue Loss after Focal Cortical Compression in Adult Rats

Sensorimotor activity has been shown to play a key role in functional outcome after extensive brain damage. This study was aimed at assessing the influence of sensorimotor experience through subject-environment interactions on the time course of both lesion and gliosis volumes as well as on the recovery of forelimb sensorimotor abilities following focal cortical injury. The lesion consisted of a cortical compression targeting the forepaw representational area within the primary somatosensory cortex of adult rats. After the cortical lesion, rats were randomly subjected to various postlesion conditions: unilateral C5–C6 dorsal root transection depriving the contralateral cortex from forepaw somatosensory inputs, standard housing or an enriched environment promoting sensorimotor experience and social interactions. Behavioral tests were used to assess forelimb placement during locomotion, forelimb-use asymmetry, and forepaw tactile sensitivity. For each group, the time course of tissue loss was described and the gliosis volume over the first postoperative month was evaluated using an unbiased stereological method. Consistent with previous studies, recovery of behavioral abilities was found to depend on post-injury experience. Indeed, increased sensorimotor activity initiated early in an enriched environment induced a rapid and more complete behavioral recovery compared with standard housing. In contrast, severe deprivation of peripheral sensory inputs led to a delayed and only partial sensorimotor recovery. The dorsal rhizotomy was found to increase the perilesional gliosis in comparison to standard or enriched environments. These findings provide further evidence that early sensory experience has a beneficial influence on the onset and time course of functional recovery after focal brain injury