Evaluation of population growth of daphnia magna feeding Ulva lactuca

Success in the fish hatchery is influenced by natural feed. One of the natural freshwater feeds that have a high nutritional content and is easy to cultivate is Daphnia magna. The availability of D. magna at any time must be supported by daily feeding. Ulva lactuca macroalgae is one of the ingredients that have the potential to be used as D. magna feed because it has good nutrition and is abundantly available on the coast. This research was conducted for 17 days at the Integrated Laboratory of Madako Tolitoli University. The aim of the study was to determine the population growth of D. magna fed          U. lactuca. D. magna was reared in a 25 x 25 x 30 cm aquarium containing 5 liters of green water and a stocking density of 20 individuals/L. The treatments tried were Treatment A = Without U. lactuca Soak (0 ml/L), Treatment B = U. lactuca Soak (3 ml/L), Treatment C = U. lactuca Soak (5 ml/L). The results showed that the administration of U. lactuca 5 ml/L (804.44 ind/L) had the highest population growth compared to the treatment of 3 ml/L immersion of U. lactuca (597.78 ind/L) and without administration of immersion of U. lactuca (482.22 ind/L). Treatment of U. Lactva 5 ml/L is effective in increasing Growth D. Magna and can be an alternative feed D. Magna whose availability is abundant in the coastal area.Keywords: Daphnia magna; growth; Ulva lactuc

Incubation of Oryzias woworae Eggs at Different Temperature on Embryo Development and Hatching Performance

Oryzias woworae is one of Muna Island's endemic fish, Sulawesi, which needs to be developed in cultivation and can be used as ornamental fish with high economic value. However, information on fish farming is not yet well available, especially at egg incubation temperatures. This study aims to determine the optimal temperature of the incubation water medium for the hatching performance of O. woworae eggs. The study used experimental methods with the treatment of differences in egg incubation temperatures at 24-26°C (temperature fluctuations in the laboratory), 24°C, 28°C and 32°C. The research parameters were analyzed descriptively, namely embryogenesis, hatching time, and hatching rates. The results showed that differences in incubation temperature affected the embryogenesis of O. woworae eggs. The obtained fastest time for hatching eggs O. woworae at a temperature of 32°C with six days 5 hours 49 minutes and 100% hatching. The egg incubation temperature 24-26°C is the lowest hatching time and hatching rate, nine days 11 hours 38 minutes and 53.33%. The use of 32°C in the incubation medium is the optimal temperature to accelerate embryogenesis and the hatching rate of O. woworae eggs

Tingkah Laku Memijah, Potensi Reproduksi Ikan Betina, dan Optimasi Teknik Pemijahan Ikan Pelangi Iriatherina Werneri Meinken, 1974 [Spawning Behavior, Female Reproductive Potential And Breeding Technique Optimize Of Threadfin Rainbowfish...............

Informasi reproduksi ikan pelangi Iriatherina werneri pada wadah terkontrol masih sedikit diketahui. Oleh karena itu, diperlukan kajian mengenai tingkah laku memijah, potensi reproduksi ikan betina berdasarkan perbedaan pakan (buatan dan alami), dan optimasi teknik pemijahan. Kajian optimasi pemijahan meliputi pengamatan pengaruh perbedaan sistem pemijahan (massal atau individual), perbedaan rasio kelamin pemijahan jantan : betina (1:1, 1:2, dan 1:3), serta perbedaan ukuran betina (kecil, sedang, dan besar) untuk mendukung kegiatan budi daya. Hasil penelitian menunjukkan bah-wa pemijahan ikan pelangi terjadi pada 13-15 jam sejak pemasangan ikan jantan dan betina yang diawali oleh gerakan ikan jantan mengembangkan dan menguncupkan sirip. Telur yang dikeluarkan pada pemijahan massal berakhir dua jam lebih cepat dibandingkan pemijahan individual dan telur lebih serempak dikeluarkan pagi hari (94,92%). Ikan pelangi merupakan pemijah bertahap yang mampu memijah setiap hari selama 30 hari. Potensi jumlah telur dan larva yang di-hasilkan seekor betina dapat ditingkatkan masing-masing sebanyak empat kali lipat dan 14 kali lipat melalui pemberian pakan alami. Optimasi teknik pemijahan I. werneri dicapai dengan menggunakan sistem massal dengan rasio kelamin 1: 3 dan menggunakan ikan betina berukuran 26,98-35,76 mm

Rancang Bangun Sistem Monitoring Kualitas Air untuk Pemeliharaan Organisme Laut

Kualitas air pada wadah pemeliharaan dan budidaya organisme laut merupakan aspek penting yang harus diperhatikan. Mekanisme pemeliharaan dan kontrol kualitas air laut lebih kompleks dibandingkan dengan air tawar karena lebih rentan terhadap cemaran yang berasal dari sisa metabolisme atau sisa makanan. Kemajuan teknologi saat ini memungkinkan untuk melakukan manajemen kualitas air secara terkontrol sehingga lebih efisien. Pengembangan sistem monitoring kualitas air dapat dilakukan menggunakan mikrokontrol arduino yang dilengkapi dengan sensor suhu, DO (dissolved oxygen) dan salinitas. Ketiga parameter tersebut menjadi pembatas utama bagi kelangsungan hidup organisme air laut. Hasil penelitian menunjukkan bahwa sistem yang dibangun dapat bekerja baik sesuai dengan perubahan kondisi parameter pengukuran dengan tingkat kesalahan 0,8-3,8%. Kebutuhan listriknya yang rendah (9 volt) memungkinkan variasi sumber energi yang dapat digunakan antara lain aliran listrik negara, generator, baterai, maupun sumber energi terbarukan seperti energi matahari atau energi angin. Meskipun demikian, keandalan sistem monitoring yang dibangun memerlukan validasi di lapangan sebelum diterapkan pada lingkungan yang sebenarnya

Early Study on Embryogenesis O.woworae at Different Salinities

Oryzias woworae is an endemic fish of Sulawesi. This endemic fish naturally had faced endangered illegal and unlawful capture, therefore it is needed to be protected and conserved by a cultivation system. This research aims to provide basic information related to the cultivation of O. woworae to know the optimum salinity of hatching media and its influence on the hatchability of eggs and the length of time hatching O. woworae. This research was conducted using experimental methods and presented descriptively. The treatment used in this study is 0 ppt, 2-4 pt, 6-8 ppt, 10-12 ppt. The results showed that O. woworae eggs can hatch in salinity 0 ppt until 10-12 ppt and the best salinity for the length of hatching time is treatment of 2-4 ppt which is 7th day 14 hours 20 minutes. Treatment 0 ppt, 2-4 ppt and 6-8 ppt can reach 100%. This can be concluded that the optimum salinity of the hatching rate of Oryzias woworae until 6-8 ppt, but in treatment 10-12 ppt hatchability only gets 80% as some embryos become shrinking. Hatchability per day shows the treatment of 2-4 ppt is the best treatment, on the 7th day as much as 40% and on the 8th day as much as 40%. The optimal salinity for hatching O. woworae eggs is  2-4 ppt treatment, salinity that can expedite the length of hatching time and produce  hatching rate by 100%. 

Teknik Produksi Naupli Udang Vaname (Litopenaeus vannamei) di PT. Tri Karta Pratama, Carita, Pandeglang, Banten

Udang vaname (Litopenaeus vannamei) merupakan salah satu produk ekspor unggulan perikanan budidaya di Indonesia. Keunggulan udang vaname antara lain pertumbuhannya cepat, dapat dibudidayakan dengan kepadatan tinggi, memiliki sintasan yang tinggi, dan mempunyai harga pasar yang cukup tinggi. Salah satu indikator keberhasilan pembenihan udang vaname adalah ketersediaan benur yang berkualitas dan berkesinambungan. Keberhasilan dalam kegiatan pembenihan perlu didukung dengan pengetahuan tentang teknik produksi naupli udang vaname. Karena itu, kegiatan ini bertujuan untuk mengkaji teknik produksi naupli udang vaname di PT. Tri Karta Pratama. Metode yang digunakan untuk memperoleh data primer dalam kegiatan ini adalah mengikuti secara langsung, mengamati dan melakukan wawancara terkait produksi naupli. Data sekunder diperoleh melalui penelusuran literatur terkait produksi naupli vaname. Teknik produksi naupli vaname meliputi persiapan media pemeliharaan induk, pemeliharaan dan pematangan induk, seleksi induk matang gonad, pemijahan induk, dan penetasan telur. Secara umum kegiatan produksi naupli udang vaname di PT. Tri Karta Pratama sesuai dengan prosedur standar operasional yang ditetapkan

Spawning activity of Oryzias woworae Parenti & Hadiaty 2010 with the Suplementation Spirulina Meal in the Diet

Spirulina platensis is a feed additive that can improve the reproductive performance of fish. It has never been given to the endemic fish Oryzias woworae which can lay eggs every day. Therefore, a study was conducted that aims to examine spawning activities related to egg-laying behavior, number of eggs, and egg diameter of O. woworae. The study used a sex ratio of 1♂:1♀ in spawning. The test feeds were commercial feed (Feng Li 1) or 0% without spirulina (treatment A), the addition of spirulina 5% (treatment B), 10% (treatment C), and 20% (treatment D). Observations were made every hour for 6 days. The spawning temperature was controlled at a value of 26.6±0.2°C. The results showed differences in the behavior of O. woworae in releasing eggs on spawning days. Giving spirulina causes the behavior of releasing eggs to occur at intervals of 1-3 hours, while without spirulina, it only occurs 1 hour on spawning days. The highest number of eggs was produced by giving spirulina 20% with a diameter range of 1.35-1.37 mm. The presence of a small egg diameter in the 20% spirulina treatment indicated that an increase in the number of eggs affected the ovaries capacity, so the diameter decreased. However, the average diameter of 20% of spirulina eggs was still the same as without spirulina administration. The stable temperature at 26.6±0.2°C caused the spawning activity of O. woworae to only occur in the morning between 07:00-09:00

Growth Performance of Daphnia sp. Cultured in Different Concentration of Rice Washing Water

Abstract. This study aimed to investigate the effect of the administration of rice washing water in culture medium on the growth performance of Daphnia sp. This research using three different doses of rice washing water i.e 1 mL/L, 3mL/L, and 5 mL/L. Daphnia sp. was cultured with an initial density of 20 ind/L. Observed parameters include growth parameters (population density, size and specific growth rate) and water quality. This result showed that a concentration of 3 mL/L created the highest population of Daphnia sp. density about 620±20 ind/L, number small size (young stage) 81.2%, and the highest specific growth rate about 56.68±0.55%. The water quality content of  DO, temperature and pH during this study were in the good range of Daphnia sp. life and reproduction. The research has a conclusion that rice washing water can be used to nutritional sources of Daphnia sp. In the future, it is necessary to make further observations about the reproductive performance of Daphnia sp, given rice washing water through clone culture.Keywords: Live food, Population density, Rice washing water, Size of Daphnia sp., Specific growth rat

Antibakteri Staphylococcus aureus dan Escherichia coli dari Kombucha Rumput Laut Merah (Eucheuma spinosum) Karangantu Banten dan Antifungi Curvularia pseudorobusta Pada Ikan Mas Koki

Rumput laut merah (Eucheuma spinosum)dari kawasan Karangantu Provinsi Banten menjadi objek pada penelitian ini. Tujuan riset ini untuk memberikan informasi secara ilmiah kemampuan E. spinosum setelah difermentasi oleh kombucha untuk menghambat pertumbuhan bakteri gram positif Staphylococcus aureus dan Escherichia coli, serta fungi penyebab penyakit ikan mas koki Curvularia pseudorobusta. Hasil penelitian membuktikan kombucha yang berbahan dasar rumput laut merah (E. spinosum)secara signifikan dapat menghambat pertumbuhan ketiga mikroba uji. Hasil uji ANOVA satu arah tiap nilai menunjukkan P <0,05 dengan hasil analisis pos hoc kombucha rumput laut merah konsentrasi gula 40% berbeda nyata dengan konsentrasi gula 20% dan 30%. Konsentrasi gula 40% menghasilkan diameter zona hambat sebesar 13,30 mm pada S. aureus dan E. coli sebesar 10,35 mm dengan kategori kuat. Pertumbuhan C. pseudorobusta menghasilkan zona hambat pada konsentrasi gula 40% sebesar 10,02 mm. Kesimpulannya bahwa kombucha berbahan dasar rumput laut merah (E. spinosum) berasal dari Karangantu mempunyai daya lebih peka terhadp S. aureus dibandingkan E. coli dan fungi patogen C. pseudorobusta.Kata kunci: antimikroba, karangantu, rumput lau

IKTIOFAUNA AIR TAWAR BEBERAPA DANAU DAN SUNGAI INLETNYA DI PROVINSI SULAWESI TENGAH, INDONESIA

Sulawesi Tengah Province (Sulteng) is in the Wallacea region wich have endemic fish. In addition, the government has also introduced fish for the welfare of community. So far the records of freshwater fish in Sulteng have not been well summarized. Therefore, we explore the results of previous studies fish species in 11 lakes and their inlet rivers in Sulteng. The lake (L) is L. Bolano (Bolanosau), L. Lindu, L. Poso, L. Rano, L. Rano Kodi and L. Rano Bae, L. Sibili, L. Talaga (Dampelas), L. Kalimpa’a (Tambing), L. Tiu and L. Wanga. In addition, we also observed fish in seven lakes between 2012-2019. Fishing uses cast net, seine net, gillnet, and hook and line. Summary and observation result showed that there were 18 families and 27 genera of fish in 11 lake and their inlet rivers in Sulteng. Then there are 15 endemic species in 3 habitats (Lindu, Poso and Tiu), namely Adrianichthys 4 species, Oryzias 6 species, Mugilogobius 2 species and Nomorhamphus 3 species. Introduced fish as many as 23 species, Oreochromis niloticus the most found (8 lakes). Lake Poso (30 species) has the most fish species. Utilization of fish in 11 lake as consumption fish and ornamental fish. Especially for endemic fish, in situ (habitat) and / or ex situ (aquaculture) conservation needs to be carried out in order to remain sustainable. Through aquaculture, the breeding of endemic species that have the potential as ornamental fish and/or consumption can be avoided from exploitation (overfishing) in nature.Provinsi Sulawesi Tengah (Sulteng) berada dalam kawasan Wallacea memiliki ikan endemik di danau serta sungai inletnya. Selain itu, pemerintah juga telah melakukan introduksi ikan ke perairan umum untuk kesejahteraan masyarakat. Sejauh ini catatan iktiofauna air tawar di Sulteng belum terangkum dengan baik. Oleh karena itu, kami menelusuri hasil penelitian terdahulu tentang jenis ikan di 11 danau dan sungai inletnya di&nbsp; Sulteng. Danau (D) tersebut yaitu D. Bolano (Bolanosau), D. Lindu, D. Poso, D. Rano, D. Rano Kodi dan D. Rano Bae, Danau Sibili, D. Talaga (Dampelas), D. Kalimpa’a (Tambing), D. Tiu dan D. Wanga. Selain itu, kami juga melakukan pengamatan ikan di tujuh danau antara tahun 2012-2019. Penangkapan ikan menggunakan jaring lempar, jaring pantai, pukat insang dan pancing. Hasil rangkuman dan pengamatan menunjukkan bahwa terdapat 18 famili dan 27 genus ikan di 11 danau dan sungai inletnya di Sulteng. Kemudian terdapat 15 spesies endemik di 3 danau dan sungai inletnya (Lindu, Poso dan Tiu) yaitu Adrianichthys 4 spesies, Oryzias 6 spesies, Mugilogobius 2 spesies, Nomorhamphus 3 spesies. Ikan introduksi sebanyak 23 spesies, Oreochromis niloticus yang paling banyak ditemukan (8 danau). Danau Poso (30 spesies) memiliki iktiofauna terbanyak. Pemanfaatan terhadap ikan di 11 danau tersebut sebagai ikan konsumsi dan ikan hias. Khusus ikan endemik, perlu dilakukan konservasi secara in situ (habitat) dan / atau ex situ (akuakultur) agar tetap lestari. Melalui akuakultur, pengembangbiakan spesies endemik yang berpotensi sebagai ikan hias dan/atau konsumsi dapat dihindarkan dari eksploitasi (penangkapan berlebihan) di alam