An ultrastructural study of intramuscular melanocytes in PET/Wmr mice

An examination of the lateral head of the gastrocnemius of the PET/Wn!.r mouse was made with an electron microscope. Cells of the perimysium and endomysium were described and the relationship of these cells to muscle cells was characterized. The relationship between melanocytes and muscle cells found in this strain was the major focus. Melanocytes were seen to be present in the perimysium and endomysium with the greatest concentration in the endomysium. Melanocytes of the perimysium appeared segregated from the adjacent tissue by fibroblasts,which surround ed the melanocytes with their cytoplasmic processes, a well as by collagen fibers. Melanocytes of the endomysium were seen to have no direct communication with the muscle cells though there were conical protrusions of the sarcolemma at areas of juxtaposition between the perikaryon of the melanocytes and the muscle cells

Preliminary design studies of an advanced general aviation aircraft

The preliminary design results are presented of the advanced aircraft design project. The goal was to take a revolutionary look into the design of a general aviation aircraft. Phase 1 of the project included the preliminary design of two configurations, a pusher, and a tractor. Phase 2 included the selection of only one configuration for further study. The pusher configuration was selected on the basis of performance characteristics, cabin noise, natural laminar flow, and system layouts. The design was then iterated to achieve higher levels of performance

The Grey Web: dataveillance vision fulfilled through the evolving Web

Over the past three decades, Web has evolved from an information medium to an intricate economic ecosystem. Initially focused on supporting the transition from traditional business practices to e-commerce, the Web has given rise to new, purely Web based businesses. Aligned with the original vision and expectations of the ‘free Web’, they have provided free services but, over time, developed business models that leverage the user digital footprints and the user generated content to create economic value. With the use of computing technologies to analyze, aggregate, and share such data, individuals’ privacy has been undermined and, with that, the their ability to shape their role in the digital society and beyond. The purpose of this paper is to instigate the dialogue around the critical societal issues that arise from the current Web economy and motivate research initiatives to assist with addressing them. We present three case studies that quantify the extent, rate, and pervasiveness of the user tracking on the Web. We use them to illustrate the determining aspects of the Web that have to be taken into account by the Web Science community. As researchers we aspire to understand the nature of the Web in depth and, based on that, propose designs and policies that are required to ensure that the Web is fit to be the underpinning of our societies and our digital future

A Characterization of the Chevalley Groups G(,2)(2('n))

82 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1968.U of I OnlyRestricted to the U of I community idenfinitely during batch ingest of legacy ETD

Network analysis of third party tracking: user exposure to tracking cookies through search

Internet advertisers reach millions of customers through practices that real time tracking of users’ online activities. The tracking is conducted by third party ad services engaged by the Web sites to facilitate marketing campaigns. Previous research has investigated tracking practices and tracking agencies associated with popular Web sites. Here we investigate the network properties of the third party referral structures that facilitate gathering of user information for the delivery of personalized ads. By considering third party domains associated with the top ten search results for a diverse set of queries, we arrived at the networks of third party domains in four search markets. We show a consistent network structure across markets, with a dominant connected component that, on average, includes 92.8% of network vertices and 99.8% of the connecting edges. There is 99.5% chance that a user will become tracked by all top 10 trackers within 30 clicks on search results. Finally, the third party networks exhibit properties of the small world networks. This implies a high-level global and local efficiency in spreading the user information and delivering targeted ads

Targeting Survivin Enhances Chemosensitivity in Retinoblastoma Cells and Orthotopic Tumors

<div><p>Treatments for retinoblastoma (Rb) vary depending on the size and location of the intraocular lesions and include chemotherapy and radiation therapy. We examined whether agents used to treat Rb induce a pro-survival phenotype associated with increased expression of survivin, a member of the inhibitor of apoptosis family of proteins. We document that exposure to carboplatin, topotecan or radiation resulted in elevated expression of survivin in two human Rb cell lines but not in normal retinal pigmented epithelial (RPE) cells. Cellular levels of survivin were attenuated in Rb cells exposed to an imidazolium-based survivin suppressant, Sepantronium bromide (YM155). Protein expression patterns of survivin in RPE cells were not altered following treatment protocols involving exposure to YM155. Including YM155 with chemotherapy or radiation increased levels of apoptosis in Rb cells but not in RPE cells. Intraocular luciferase expressing Rb tumors were generated from the Rb cell lines and used to evaluate the effects of carboplatin and YM155 on in-vivo survivin expression and tumor growth. Carboplatin induced expression of survivin while carboplatin combined with YM155 reduced survivin expression in tumor bearing eyes. The combination protocol was also most effective in reducing the rate of tumor regrowth. These results indicate that targeted inhibition of the anti-apoptotic protein survivin provides a therapeutic advantage for Rb cells and tumors treated with chemotherapy.</p></div

Targeting Survivin Enhances Chemosensitivity in Retinoblastoma Cells and Orthotopic Tumors - Fig 2

<p><b>YM155 enhances apoptosis and decreases viability in Y79 (A) and CHLA-215 (B) Rb cells exposed to chemotherapy or radiation.</b> YM155 decreased viability in RPE (C) cells and induced only a minimal apoptotic response in these cells following radiation. Percent apoptotic cells was determined by FACS analysis of annexin V and PI stained cells either under control or treatment conditions described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0153011#pone.0153011.g001" target="_blank">Fig 1</a>. <i>Columns</i>, mean; <i>bars</i>, ± SE of three to five separate experiments. **<i>P</i> < 0.05 (Control vs CBDCA, TOPO, or Rad); *<i>P</i> < 0.05 (Control, CBDCA, TOPO or Rad in the absence of YM155 vs Control, CBDCA, TOPO, or Rad in the presence of YM155).</p