Traçabilité dans la filière viande. I. La traçabilité administrative.

peer reviewedLe secteur de la viande a été secoué ces dernières années par quelques scandales, tels ceux des hormones et de la dioxine, avec pour conséquences une perte de confiance de la part du consommateur et une perturbation du marché de la viande. Pour redresser l’image des produits carnés belges, il est important de pouvoir en déterminer et en garantir l’origine. En Belgique, il existe divers systèmes de traçabilité administrative dont le principal est le système SANITEL qui comprend un système automatisé de traitement de données relatives à l’identification et l’enregistrement des animaux. Au-delà de l’aspect légal et réglementaire, différentes initiatives, visant une amélioration de la qualité, fleurissent : "les labels". Ceux-ci intègrent fréquemment la traçabilité dans leur cahier des charges. La traçabilité administrative n’est pas infaillible, la perte de documents et les fraudes peuvent ternir l’image de celle-ci. C’est pourquoi le système documentaire a été associé aux empreintes génétiques des animaux.

Proper Orthogonal Decomposition Analysis and Modelling of the wake deviation behind a squareback Ahmed body

We investigate numerically the 3-D flow around a squareback Ahmed body at Reynolds number Re = 104. Proper Orthogonal Decomposition (POD) is applied to a symmetry-augmented database in order to describe and model the flow dynamics. Comparison with experiments at a higher Reynolds number in a plane section of the near-wake at mid-height shows that the simulation captures several features of the experimental flow, in particular the antisymmetric quasi-steady deviation mode. 3-D POD analysis allows us to classify the different physical processes in terms of mode contribution to the kinetic energy over the entire domain. It is found that the dominant fluctuating mode on the entire domain corresponds to the 3-D quasi-steady wake deviation, and that its amplitude is well estimated from 2-D near-wake data. The next most energetic flow fluctuations consist of vortex shedding and bubble pumping mechanisms. It is found that the amplitude of the deviation is negatively correlated with the intensity of the vortex shedding in the spanwise direction and the suction drag coefficient. Finally, we find that despite the slow convergence of the decomposition, a POD-based low-dimensional model reproduces the dynamics of the wake deviation observed experimentally, as well as the main characteristics of the global modes identified in the simulation

Fluid force and symmetry breaking modes of a 3D bluff body with a base cavity

International audienceA cavity at the base of the squareback Ahmed model at Re 4 × 10 5 is able to reduce the base suction by 18% and the drag coefficient by 9%, while the geometry at the separation remains unaffected. Instantaneous pressure measurements at the body base, fluid force measurements and wake velocity measurements are investigated varying the cavity depth from 0 to 35% of the base height. Due to the reflectional symmetry of the rectangular base, there are two Reflectional Symmetry Breaking (RSB) mirror modes present in the natural wake that switch from one to the other randomly in accordance with the recent findings of Grandemange et al. (2013b). It is shown that these modes exhibit an energetic 3D static vortex system close to the base of the body. A sufficiently deep cavity is able to stabilize the wake toward a symmetry preserved wake, thus suppressing the RSB modes and leading to a weaker elliptical toric recirculation. The stabilization can be modelled with a Langevin equation. The plausible mechanism for drag reduction with the base cavity is based on the interaction of the static 3D vortex system of the RSB modes with the base and their suppression by stabilization. There are some strong evidences that this mechanism may be generalized to axisym-metric bodies with base cavity

Proper Orthogonal Decomposition Analysis and Modelling of the wake deviation behind a squareback Ahmed body

We investigate numerically the 3-D flow around a squareback Ahmed body at Reynolds number Re = 104. Proper Orthogonal Decomposition (POD) is applied to a symmetry-augmented database in order to describe and model the flow dynamics. Comparison with experiments at a higher Reynolds number in a plane section of the near-wake at mid-height shows that the simulation captures several features of the experimental flow, in particular the antisymmetric quasi-steady deviation mode. 3-D POD analysis allows us to classify the different physical processes in terms of mode contribution to the kinetic energy over the entire domain. It is found that the dominant fluctuating mode on the entire domain corresponds to the 3-D quasi-steady wake deviation, and that its amplitude is well estimated from 2-D near-wake data. The next most energetic flow fluctuations consist of vortex shedding and bubble pumping mechanisms. It is found that the amplitude of the deviation is negatively correlated with the intensity of the vortex shedding in the spanwise direction and the suction drag coefficient. Finally, we find that despite the slow convergence of the decomposition, a POD-based low-dimensional model reproduces the dynamics of the wake deviation observed experimentally, as well as the main characteristics of the global modes identified in the simulation

The ReproGenomics Viewer: an integrative cross-species toolbox for the reproductive science community.

International audienceWe report the development of the ReproGenomics Viewer (RGV), a multi-and cross-species working environment for the visualization, mining and comparison of published omics data sets for the reproductive science community. The system currently embeds 15 published data sets related to gametogenesis from nine model organisms. Data sets have been curated and conveniently organized into broad categories including biological topics, technologies, species and publications. RGV's modular design for both organisms and genomic tools enables users to upload and compare their data with that from the data sets embedded in the system in a cross-species manner. The RGV is freely available at http://rgv.genouest.org

Dynamics of the transcriptional landscape during human fetal testis and ovary development

Acknowledgements We thank all members of the SEQanswers forums for helpful advice; Steven Salzberg and Cole Trapnell for continuous support with the ‘Tuxedo’ suite; and the UCSC Genome team members. Sequencing was performed by the GenomEast platform, a member of the ‘France Génomique’ consortium (ANR-10-INBS-0009). We thank Ms Linda Robertson, Ms Margaret Fraser, Ms Samantha Flannigan (University of Aberdeen) and the staff at Grampian NHS Pregnancy Counselling Service and all the staff of the Department of Obstetrics and Gynecology of the Rennes Sud Hospital for their expert assistance and help, and the participating women, without whom this study would not have been possible. The authors are grateful for Ms Gersende Lacombe and Mr Laurent Deleurme from the Biosit CytomeTri cytometry core facility of Rennes 1 University. Funding French National Institute of Health and Medical Research (Inserm); University of Rennes 1; French School of Public Health (EHESP); Swiss National Science Foundation [SNF n° CRS115_171007 to B.J.]; the French National Research Agency [ANR n° 16-CE14-0017-02 and n°18-CE14-0038-02 to F.C]; Medical Research Council [MR/L010011/1 to PAF]; European Community’s Seventh Framework Programme (FP7/2007–2013) [under grant agreement no 212885 to PAF]; European Union’s Horizon 2020 Research and Innovation Programme [under grant agreement no 825100 to P.A.F. and S.M.G.].Peer reviewedPostprin

FIREBall-2: flight preparation of a proven balloon payload to image the intermediate redshift circumgalactic medium

FIREBall-2 is a stratospheric balloon-borne 1-m telescope coupled to a UV multi-object slit spectrograph designed to map the faint UV emission surrounding z~0.7 galaxies and quasars through their Lyman-alpha line emission. This spectro-imager had its first launch on September 22nd 2018 out of Ft. Sumner, NM, USA. Because the balloon was punctured, the flight was abruptly interrupted. Instead of the nominal 8 hours above 32 km altitude, the instrument could only perform science acquisition for 45 minutes at this altitude. In addition, the shape of the deflated balloon, combined with a full Moon, revealed a severe off-axis scattered light path, directly into the UV science detector and about 100 times larger than expected. In preparation for the next flight, and in addition to describing FIREBall-2's upgrade, this paper discusses the exposure time calculator (ETC) that has been designed to analyze the instrument's optimal performance (explore the instrument's limitations and subtle trade-offs)

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer‐reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state‐of‐the‐art handbook for basic and clinical researchers.DFG, 389687267, Kompartimentalisierung, Aufrechterhaltung und Reaktivierung humaner Gedächtnis-T-Lymphozyten aus Knochenmark und peripherem BlutDFG, 80750187, SFB 841: Leberentzündungen: Infektion, Immunregulation und KonsequenzenEC/H2020/800924/EU/International Cancer Research Fellowships - 2/iCARE-2DFG, 252623821, Die Rolle von follikulären T-Helferzellen in T-Helferzell-Differenzierung, Funktion und PlastizitätDFG, 390873048, EXC 2151: ImmunoSensation2 - the immune sensory syste