Selective extraction of intracellular components from the microalga Chlorella vulgaris by combined pulsed electric field–temperature treatment

The synergistic effect of temperature (25–65 C) and total specific energy input (0.55–1.11 kWh kgDW 1 ) by pulsed electric field (PEF) on the release of intracellular components from the microalgae Chlorella vulgaris was studied. The combination of PEF with temperatures from 25 to 55 C resulted in a conductivity increase of 75% as a result of cell membrane permeabilization. In this range of temperatures, 25–39% carbohydrates and 3–5% proteins release occurred and only for carbohydrate release a synergistic effect was observed at 55 C. Above 55 C spontaneous cell lysis occurred without PEF. Combined PEF–temperature treatment does not sufficiently disintegrate the algal cells to release both carbohydrates and proteins at yields comparable to the benchmark bead milling (40–45% protein, 48–58% carbohydrates)

Design of value chains for microalgal biorefinery at industrial scale : process integration and techno-economic analysis

The objective of this work was to identify industrial scenarios for the most promising microalgal biorefinery value chains on the basis of product selection, yields, and techno-economic performance, using biological characteristics of algae species. The development, value creation, and validation of several new processing routes with applications in food, aquafeeds and non-food products were particularly considered in this work. The techno-economic performance of various single product value chains (SP) and multiproduct value chains (MP) was evaluated for four industrial microalgal strains. Cost-revenue optimization was done for a 10 kton microalgal dry weight y–1 simulated biorefinery plant, using flow sheeting software for equipment sizing, mass and energy flow modeling, and subsequent techno-economic evaluation. Data on yield, material and energy consumption were based on pre- and pilot size production plants (TRL 5–6). Revenue optimization was accomplished by first analyzing the performance of single product value chains of the microalgal strains. Subsequently, a strategy was developed to exploit almost all biomass based on the most promising microalgal strains. The cultivation costs are most of the time the major costs of the value chains. For the single product value chains common process bottlenecks are low product yields, especially for soluble proteins where only a small fraction of the biomass is leading to economic value. The biorefinery costs (excluding cultivation) vary significantly for various species, due to the species-specific operating conditions as well as differences in product yields. For the evaluated single product value chain scenarios the costs for utilities and other inputs were in general the highest contributing expenses. A biorefinery approach significantly increases the biomass utilization potential to marketable products from 7–28% to more than 97%. Although the cascading approach increases the total production costs of the multiproduct value chains significantly, this is more than compensated by the increased overall biomass revenue. For all selected multiproduct chains there is a significant potential to become profitable at a relevant industrial scale of 10 kton per year. Additional insights in the product functionality, quality, and their market size are needed to narrow down the wide range of foreseen product revenues and resulting profits.publishedVersio

Selective and mild fractionation of microalgal proteins and pigments using aqueous two-phase systems

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Roughness Based Crossflow Transition Control for a Swept Airfoil Design Relevant to Subsonic Transports

A high fidelity transition prediction methodology has been applied to a swept airfoil design at a Mach number of 0.75 and chord Reynolds number of approximately 17 million, with the dual goal of an assessment of the design for the implementation and testing of roughness based crossflow transition control and continued maturation of such methodology in the context of realistic aerodynamic configurations. Roughness based transition control involves controlled seeding of suitable, subdominant crossflow modes in order to weaken the growth of naturally occurring, linearly more unstable instability modes via a nonlinear modification of the mean boundary layer profiles. Therefore, a synthesis of receptivity, linear and nonlinear growth of crossflow disturbances, and high-frequency secondary instabilities becomes desirable to model this form of control. Because experimental data is currently unavailable for passive crossflow transition control for such high Reynolds number configurations, a holistic computational approach is used to assess the feasibility of roughness based control methodology. Potential challenges inherent to this control application as well as associated difficulties in modeling this form of control in a computational setting are highlighted. At high Reynolds numbers, a broad spectrum of stationary crossflow disturbances amplify and, while it may be possible to control a specific target mode using Discrete Roughness Elements (DREs), nonlinear interaction between the control and target modes may yield strong amplification of the difference mode that could have an adverse impact on the transition delay using spanwise periodic roughness elements

The Effect of Acoustic Forcing on Instabilities and Breakdown in Swept-Wing Flow over a Backward-Facing Step

Instability interaction and breakdown were experimentally investigated in the flow over a swept backward-facing step. Acoustic forcing was used to excite the Tollmien-Schlichting (TS) instability and to acquire phase-locked results. The phase-averaged results illustrate the complex nature of the interaction between the TS and stationary cross flow instabilities. The weak stationary cross flow disturbance causes a distortion of the TS wavefront. The breakdown process is characterized by large positive and negative spikes in velocity. The positive spikes occur near the same time and location as the positive part of the TS wave. Higher-order spectral analysis was used to further investigate the nonlinear interactions between the TS instability and the traveling cross flow disturbances. The results reveal that a likely cause for the generation of the spikes corresponds to nonlinear interactions between the TS, traveling cross flow, and stationary cross flow disturbances. The spikes begin at low amplitudes of the unsteady and steady disturbances (2-4% U (sub e) (i.e. boundary layer edge velocity)) but can achieve very large amplitudes (20-30 percent U (sub e) (i.e. boundary layer edge velocity)) that initiate an early, though highly intermittent, breakdown to turbulence

Fractionation of proteins and carbohydrates from crude microalgae extracts using an ionic liquid based-aqueous two phase system

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Selective and energy efficient extraction of functional proteins from microalgae for food applications

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Techno-Functional Properties of Crude Extracts from the Green Microalga Tetraselmis suecica

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Slow Molecules Produced by Photodissociation

A simple method to control molecular translation with a chemical reaction is demonstrated. Slow NO molecules have been produced by partially canceling the molecular beam velocity of NO$_2$ with the recoil velocity of the NO photofragment. The NO$_2$ molecules were photodissociated using a UV laser pulse polarized parallel to the molecular beam. The spatial profiles of NO molecules showed two peaks corresponding to decelerated and accelerated molecules, in agreement with theoretical prediction. A significant portion of the decelerated NO molecules stayed around the initial dissociation positions even several hundred nanoseconds after their production.Comment: 17 pages, 4 figure

On the effect of discrete roughness on the growth of crossflow instability in very low turbulence environment

Wind tunnel experiments were conducted in a low-turbulence environment (Tu < 0.006%) on the stability of 3D boundary layers. The effect of two different distributions of discrete roughness elements (DREs) on crossflow vortices disturbances and their growth was evaluated. As previously reported, DREs are found to be an effective tool in modulating the behaviour of crossflow modes. However, the effect of 24µm DREs was found to be weaker than previously thought, possibly due to the low level of environmental disturbances herewith. Preliminary results suggest that together with the height of the DREs and their spanwise spacing, their physical distribution across the surface also intimately affects the stability of 3D boundary layers. Finally, crossflow vortices are tracked along the chord of the model and their merging is captured. This phenomena is accompanied by a change in the critical wavelength of the dominant mode