Elaboração de algoritmo para a escolha do banho do paciente coronariopata

The aim was to apply the validated criteria and clinical parameters used by nurses in an algorithm to support the choice of bathing for patients with coronary artery disease. This is a methodological study with a quantitative approach to develop an algorithm for decision making regarding the choice of bathing in patients with coronary artery disease. It resulted in 19 validated items and 19 items suggested and used by experts. Of these, after categorization, 21 items were selected, organized in an algorithm format, with dichotomous responses that suggest, at each end of the flow, the type of bath indicated for the patient, due to their clinical conditions at the time of evaluation. In conclusion, an elaborate algorithm guides nurses in choosing the type of bath that will be offered to patients with coronary artery disease, through validated clinical parameters based on findings in the literature. This study needs algorithm validation for its clinical use.Objetivou-se aplicar os critérios validados e parâmetros clínicos utilizados por enfermeiros em um algoritmo para a apoiar a escolha do banho do paciente coronariopata. Trata-se de um estudo metodológico com abordagem quantitativa para elaboração de um algoritmo para tomada de decisão referente a escolha do banho no paciente coronariopata. Resultou-se em 19 itens validados e 19 itens sugeridos e utilizados pelos especialistas. Destes, após categorização, foram selecionados 21 itens, organizados em formato de algoritmo, com respostas dicotômicas que sugerem, a cada final de fluxo, o tipo do banho indicado ao paciente, devido às suas condições clínicas no momento da avaliação. Por conclusão, algoritmo elaborado norteia o enfermeiro na escolha do tipo do banho que será oferecido ao paciente coronariopata, através de parâmetros clínicos validados baseados nos achados da literatura. Este estudo necessita de validação do algoritmo para sua utilização clínica

Electromagnetism in terms of quantum measurements

We consider the question whether electromagnetism can be derived from quantum physics of measurements. It turns out that this is possible, both for quantum and classical electromagnetism, if we use more recent innovations such as smearing of observables and simultaneous measurability. In this way we justify the use of von Neumann-type measurement models for physical processes. We apply operational quantum measurement theory to gain insight in fundamental aspects of quantum physics. Interactions of von Neumann type make the Heisenberg evolution of observables describable using explicit operator deformations. In this way one can obtain quantized electromagnetism as a measurement of a system by another. The relevant deformations (Rieffel deformations) have a mathematically well-defined "classical" limit which is indeed classical electromagnetism for our choice of interaction

Stretching the Rules: Monocentric Chromosomes with Multiple Centromere Domains

The centromere is a functional chromosome domain that is essential for faithful chromosome segregation during cell division and that can be reliably identified by the presence of the centromere-specific histone H3 variant CenH3. In monocentric chromosomes, the centromere is characterized by a single CenH3-containing region within a morphologically distinct primary constriction. This region usually spans up to a few Mbp composed mainly of centromere-specific satellite DNA common to all chromosomes of a given species. In holocentric chromosomes, there is no primary constriction; the centromere is composed of many CenH3 loci distributed along the entire length of a chromosome. Using correlative fluorescence light microscopy and high-resolution electron microscopy, we show that pea (Pisum sativum) chromosomes exhibit remarkably long primary constrictions that contain 3-5 explicit CenH3-containing regions, a novelty in centromere organization. In addition, we estimate that the size of the chromosome segment delimited by two outermost domains varies between 69 Mbp and 107 Mbp, several factors larger than any known centromere length. These domains are almost entirely composed of repetitive DNA sequences belonging to 13 distinct families of satellite DNA and one family of centromeric retrotransposons, all of which are unevenly distributed among pea chromosomes. We present the centromeres of Pisum as novel ``meta-polycentric'' functional domains. Our results demonstrate that the organization and DNA composition of functional centromere domains can be far more complex than previously thought, do not require single repetitive elements, and do not require single centromere domains in order to segregate properly. Based on these findings, we propose Pisum as a useful model for investigation of centromere architecture and the still poorly understood role of repetitive DNA in centromere evolution, determination, and function

Cell sorting in a Petri dish controlled by computer vision.

Fluorescence-activated cell sorting (FACS) applying flow cytometry to separate cells on a molecular basis is a widespread method. We demonstrate that both fluorescent and unlabeled live cells in a Petri dish observed with a microscope can be automatically recognized by computer vision and picked up by a computer-controlled micropipette. This method can be routinely applied as a FACS down to the single cell level with a very high selectivity. Sorting resolution, i.e., the minimum distance between two cells from which one could be selectively removed was 50-70 micrometers. Survival rate with a low number of 3T3 mouse fibroblasts and NE-4C neuroectodermal mouse stem cells was 66 +/- 12% and 88 +/- 16%, respectively. Purity of sorted cultures and rate of survival using NE-4C/NE-GFP-4C co-cultures were 95 +/- 2% and 62 +/- 7%, respectively. Hydrodynamic simulations confirmed the experimental sorting efficiency and a cell damage risk similar to that of normal FACS

Privatização dos serviços de extensão rural: uma discussão (des)necessária?

The article analyzes and discusses the privatization of the rural extension services according to international literature. To start with, the subject is set in the horizon of the debate of the so called State Reform, which determined the agenda for the development since the 1980’s. Next, we show how the subject has been treated so far having the economic theory as background and we examine experiments of privatization at international level. The analysis of such experiments reveals that the early agenda for the privatization took for granted that the rural extension services − which were a responsibility of the State − would be held completely by the private sectors. However, a series of obstacles appeared and arguments for models of privatization in which the State has a main role spread over. Therefore, the exam of international experiments of privatization led to the conclusion that they followed different orientations. One set of experiments was oriented by the notion of the “Minimum State†while the other had for reference the State as a supporter for private action. The privatization is a current and relevant process and, therefore, the discussion about it is considered necessary.Rural extension, privatization, State reform, rural development., Agribusiness, Q16.,

Phytoplankton dynamics in a highly eutrophic estuary in tropical Brazil

The port of Recife in northeastern Brazil is an important ecological and social area but little is known about its environmental quality. Observations, sampling and measurements of phytoplankton were performed during spring and neap tides in the dry (January-February, 2005) and rainy (June, 2005) seasons to assess the environmental quality of the port of Recife. The area had chlorophyll-a concentrations ranging from 3.30 to 54.40 mg m-3, the highest values occurring at low tide during the dry season. A total of 129 species were identified: 53 of them in the dry season and 97 in the rainy season. Diatoms were the most diverse group and comprised 75.47% of the phytoplankton collected in the dry season and 60.82% of those collected in the rainy season. The dry season was characterized by Coscinodiscus sp. and Helicotheca tamesis; the rainy season by Oscillatoria sp. and Coscinodiscus centralis. Species diversity indices varied from 1.06 to 3.74 bits cel-1. Low indices were related to the dominance of Helicotheca tamesis, Coscinodiscus centralis, Coscinodiscus sp. and Aulacoseira granulata. Seasonal forcing, rather than the estuarine flux, determined the phytoplankton community structure. The area is exposed to seasonally varying negative impacts due to metropolitan degradation and the high level of eutrophication.Para avaliar a qualidade ambiental Porto do Recife (Nordeste do Brasil), uma área ecológica e socialmente importante mas pouco investigada, observações, medições e amostragem do fitoplâncton foram realizadas durante as marés de sizígia e quadratura no período de estiagem (janeiro-fevereiro / 2005) e chuvoso (junho/2005). A área apresentou concentração de clorofila a variando de 3,30 a 54,40 mg m-3, com maiores valores registrados durante a maré baixa no período de estiagem. Um total de 129 espécies foi identificada com 53 espécies durante o período de estiagem e 97 espécies no chuvoso. As diatomáceas formaram o grupo mais diverso, com 75,47% no período de estiagem e 60,82% no chuvoso. Coscinodiscus sp. e Helicotheca tamesis caracterizaram o período de estiagem e Oscillatoria sp. e Coscinodiscus centralis o período chuvoso. A diversidade de espécie variou de 1,06 a 3,74 bits cel-1 . Os baixos índices foram relacionados com a dominância de Helicotheca tamesis, Coscinodiscus centralis, Coscinodiscus sp. e Aulacoseira granulata. A sazonalidade determinou a estrutura da comunidade fitoplanctônica, em vez do fluxo estuarino. A área é exposta sazonalmente a vários impactos negativos, devido à degradação metropolitana e ao elevado nível de eutrofização