16,271 research outputs found
Simultaneous release of glutamate and acetylcholine from single magnocellular "cholinergic" basal forebrain neurons
Basal forebrain (BF) neurons provide the principal cholinergic drive to the hippocampus and cortex. Their degeneration is associated with the cognitive defects of Alzheimer's disease. Immunohistochemical studies suggest that some of these neurons contain glutamate, so might also release it. To test this, we made microisland cultures of single BF neurons from 12- to 14-d-old rats. Over 1-8 weeks in culture, neuronal processes made autaptic connections onto the neuron. In 34 of 36 cells tested, a somatically generated action potential was followed by a short-latency EPSC that was blocked by 1 mM kynurenic acid, showing that they released glutamate. To test whether the same neuron also released acetylcholine, we placed a voltage-clamped rat myoball expressing nicotinic receptors in contact with a neurite. In six of six neurons tested, the glutamatergic EPSC was accompanied by a nicotinic (hexamethonium-sensitive) myoball current. Stimulation of the M-2-muscarinic presynaptic receptors ( characterized using tripitramine and pirenzepine) produced a parallel inhibition of autaptic glutamatergic and myoball nicotinic responses; metabotropic glutamate receptor stimulation produced similar but less consistent and weaker effects. Atropine enhanced the glutamatergic EPSCs during repetitive stimulation by 25 +/- 6%; the anti-cholinesterase neostigmine reduced the train EPSCs by 37 +/- 6%. Hence, synaptically released acetylcholine exerted a negative-feedback inhibition of coreleased glutamate. We conclude that most cholinergic basal forebrain neurons are capable of releasing glutamate as a cotransmitter and that the release of both transmitters is subject to simultaneous feedback inhibition by synaptically released acetylcholine. This has implications for BF neuron function and for the use of cholinesterase inhibitors in Alzheimer's disease
Environmental problems and opportunities of the peri-urban interface and their impact upon the poor
The objective of this document is to provide an overview of the problems and opportunities of the peri-urban interface (PUI) with regard to the broad concerns of environmentalsustainability and poverty
Infertility factors at the Groote Schuur Hospital Fertility Clinic
Nine hundred and four patients who visited the Fertility Clinic at Groote Schuur Hospital during 1986 were classified according to infertility factors. In 36% of all couples a male factor was present, while 57% had a tubal, 29% an ovulatory, 7% a cervical immunological and 6% a uterine factor. Four per cent of all patients had endometriosis, and 2,4% had unexplained infertility. In 57% of cases only one factor was present, but in the rest between 2 and 5 factors contributed to the infertility of the couple. The study showed that in our population the most common infertility factors are in the male and in women with tubal problems, and resources should be channelled accordingly
Photodissociation and the Morphology of HI in Galaxies
Young massive stars produce Far-UV photons which dissociate the molecular gas
on the surfaces of their parent molecular clouds. Of the many dissociation
products which result from this ``back-reaction'', atomic hydrogen \HI is one
of the easiest to observe through its radio 21-cm hyperfine line emission. In
this paper I first review the physics of this process and describe a simplified
model which has been developed to permit an approximate computation of the
column density of photodissociated \HI which appears on the surfaces of
molecular clouds. I then review several features of the \HI morphology of
galaxies on a variety of length scales and describe how photodissociation might
account for some of these observations. Finally, I discuss several consequences
which follow if this view of the origin of HI in galaxies continues to be
successful.Comment: 18 pages, 7 figures in 8 files, invited review paper for the
conference "Penetrating Bars Through Masks of Cosmic Dust: The Hubble Tuning
Fork Strikes a New Note", South Africa, June 2004. Proceedings to be
published by Kluwer, eds. D.L. Block, K.C. Freeman, I. Puerari, R. Groess, &
E.K. Bloc
Effect of Tree Phenology on LiDAR Measurement of Mediterranean Forest Structure
Retrieval of forest biophysical properties using airborne LiDAR is known to differ between leaf-on and leaf-off states of deciduous trees, but much less is understood about the within-season effects of leafing phenology. Here, we compare two LiDAR surveys separated by just six weeks in spring, in order to assess whether LiDAR variables were influenced by canopy changes in Mediterranean mixed-oak woodlands at this time of year. Maximum and, to a slightly lesser extent, mean heights were consistently measured, whether for the evergreen cork oak (Quercus suber) or semi-deciduous Algerian oak (Q. canariensis) woodlands. Estimates of the standard deviation and skewness of height differed more strongly, especially for Algerian oaks which experienced considerable leaf expansion in the time period covered. Our demonstration of which variables are more or less affected by spring-time leafing phenology has important implications for analyses of both canopy and sub-canopy vegetation layers from LiDAR surveys
Mechanical Properties of the Temporal Muscle
Tese de Mestrado Integrado. Engenharia MecĂąnica. Universidade do Porto. Faculdade de Engenharia. 201
Taylor's law and related allometric power laws in New Zealand mountain beech forests: the roles of space, time and environment
This is the author accepted manuscript. The final version is available from Wiley via https://doi.org/10.1111/oik.02622Taylor's law says that the variance of population density of a species is proportional to a power of mean population density. Densityâmass allometry says that mean population density is proportional to a power of mean biomass per individual. These power laws predict a third, varianceâmass allometry: the variance of population density of a species is proportional to a power of mean biomass per individual. We tested these laws using 10 censuses of New Zealand mountain beech trees in 250 plots over 30 years at spatial scales from 5 m to kilometers. We found that: 1) a single-species forest not disrupted by humans obeyed all three laws; 2) random sampling explained the parameters of Taylor's law at a large spatial scale in 8 of 10 censuses, but not at a fine spatial scale; 3) larger spatial scale increased the exponent of Taylor's law and decreased the exponent of varianceâmass allometry (this is the first empirical demonstration that the latter exponent depends on spatial scale), but affected the exponent of densityâmass allometry slightly; 4) despite varying natural disturbance, the three laws varied relatively little over the 30 years; 5) self-thinning and recruiting plots had significantly different intercepts and slopes of densityâmass allometry and varianceâmass allometry, but the parameters of Taylor's law were not usually significantly affected; and 6) higher soil calcium was associated with higher variance of population density in all censuses but not with a difference in the exponent of Taylor's law, while elevation above sea level and soil carbon-to-nitrogen ratios had little effect on the parameters of Taylor's law. In general, the three laws were remarkably robust. When their parameters were influenced by spatial scale and environmental factors, the parameters could not be species-specific indicators. We suggest biological mechanisms that may explain some of these findings.JEC acknowledges U.S. National Science Foundation grant DMS-1225529 and the assistance of Priscilla K. Rogerson. RBA was supported by Landcare Research. This project benefited from many years of input by staff of the former New Zealand Forest Service, Forest and Range Experiment Station, Forest Research Institute and currently Landcare Research
Stoichiometric N:P Ratios, Temperature, and Iron Impact Carbon and Nitrogen Uptake by Ross Sea Microbial Communities
The Southern Ocean is one of the most biologically important ecosystems on our planet. Microscopic plants, called phytoplankton, form the base of the food web in the Southern Ocean and play a direct role in regulating how much and how fast elements like nitrogen and carbon are cycled throughout the world ocean. The goal of this research was to determine how predicted changes in the environment will impact how fast phytoplankton use these elements. The conditions that we tested included elevated temperature, addition of iron, and the proportion of nitrogen to phosphorus in the seawater. These parameters were selected because temperatures are increasing in the Southern Ocean, and the relative availability of nutrients can alter what species of phytoplankton are present and how fast they grow. Phytoplankton were collected from two locations in the Ross Sea, Antarctica, and grown for a few weeks under experimental conditions. Our results demonstrate that all three parameters, warmer temperatures, the addition of iron, and changing nitrogen to phosphorus ratios will increase how fast phytoplankton use nitrogen and carbon, but the impact of elevated temperature and the addition of iron had a much larger impact than the nitrogen to phosphorus ratio
The assessment of science: the relative merits of post- publication review, the impact factor, and the number of citations
The assessment of scientific publications is an integral part of the scientific process. Here we investigate three methods of assessing the merit of a scientific paper: subjective post-publication peer review, the number of citations gained by a paper, and the impact factor of the journal in which the article was published. We investigate these methods using two datasets in which subjective post-publication assessments of scientific publications have been made by experts. We find that there are moderate, but statistically significant, correlations between assessor scores, when two assessors have rated the same paper, and between assessor score and the number of citations a paper accrues. However, we show that assessor score depends strongly on the journal in which the paper is published, and that assessors tend to over-rate papers published in journals with high impact factors. If we control for this bias, we find that the correlation between assessor scores and between assessor score and the number of citations is weak, suggesting that scientists have little ability to judge either the intrinsic merit of a paper or its likely impact. We also show that the number of citations a paper receives is an extremely error-prone measure of scientific merit. Finally, we argue that the impact factor is likely to be a poor measure of merit, since it depends on subjective assessment. We conclude that the three measures of scientific merit considered here are poor; in particular subjective assessments are an error-prone, biased, and expensive method by which to assess merit. We argue that the impact factor may be the most satisfactory of the methods we have considered, since it is a form of pre-publication review. However, we emphasise that it is likely to be a very error-prone measure of merit that is qualitative, not quantitative
Binary Collisions and the Slingshot Effect
We derive the equations for the gravity assist manoeuvre in the general 2D
case without the constraints of circular planetary orbits or widely different
masses as assumed by Broucke, and obtain the slingshot conditions and maximum
energy gain for arbitrary mass ratios of two colliding rigid bodies. Using the
geometric view developed in an earlier paper by the authors the possible
trajectories are computed for both attractive or repulsive interactions
yielding a further insight on the slingshot mechanics and its parametrization.
The general slingshot manoeuvre for arbitrary masses is explained as a
particular case of the possible outcomes of attractive or repulsive binary
collisions, and the correlation between asymptotic information and orbital
parameters is obtained in general.Comment: 12 pages, 7 figures, accepted for publication Dec'07, Celestial
Mechanics and Dynamical Astronom
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