535 research outputs found
Molecular Characterizations of a Novel Putative DNA-Binding Protein LvDBP23 in Marine Shrimp L. vannamei Tissues and Molting Stages
Litopenaeus Vannamei, well known as pacific white shrimp, is the most popular shrimp in the world shrimp market. Identification and characterization of shrimp muscle regulatory genes are not only important for shrimp genetic improvement, but also facilitate comparative genomic tools for understanding of muscle development and regeneration.A novel mRNA encoding for a putative DNA-binding protein LvDBP23 was identified from Litopenaeus vannamei abdominal muscle cDNA library. The LvDBP23 cDNA contains 639 nucleotides of protein-coding sequence with deduced 212 amino acids of predicted molecular mass 23.32 kDa with glycine-rich domain at amino acid position 94-130. The mRNA sequence is successfully used for producing LvDBP23 recombinant protein in sf9 insect cell expression system. The expression of LvDBP23 mRNA is presented in abdominal muscle and swimming leg muscle, as well as other tissues including intestine, lymphoid and gill. The mRNA expression has the highest level in abdominal muscle in all tested tissues. LVDBP23 transcript during the molt cycle is highly expressed in the intermolt stage. In vitro nucleic acid-binding assays reveal that LvDBP23 protein can bind to both ssDNA and dsDNA, indicating its possible role of regulation of gene transcription.We are the first to report a DNA-binding protein identified from the abdominal muscle tissue of marine shrimp L. Vannamei. Its high-level specific expression during the intermot stage suggests its role in the regulation of muscle buildup during the growth phase of shrimp molt cycle
Multimodal evaluation of ultra-short laser pulses treatment for skin burn injuries
FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORFAPEAM - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DO AMAZONASThousands of people die every year from burn injuries. The aim of this study is to evaluate the feasibility of high intensity femtosecond lasers as an auxiliary treatment of skin burns. We used an in vivo animal model and monitored the healing process using 4 different imaging modalities: histology, Optical Coherence Tomography (OCT), Second Harmonic Generation (SHG), and Fourier Transform Infrared (FTIR) spectroscopy. 3 dorsal areas of 20 anesthetized Wistar rats were burned by water vapor exposure and subsequently treated either by classical surgical debridement, by laser ablation, or left without treatment. Skin burn tissues were non-invasively characterized by OCT images and biopsied for further histopathology analysis, SHG imaging and FTIR spectroscopy at 3, 5, 7 and 14 days after burn. The laser protocol was found as efficient as the classical treatment for promoting the healing process. The study concludes to the validation of femtosecond ultra-short pulses laser treatment for skinburns, with the advantage of minimizing operatory trauma. (C) 2017 Optical Society of AmericaThousands of people die every year from burn injuries. The aim of this study is to evaluate the feasibility of high intensity femtosecond lasers as an auxiliary treatment of skin burns. We used an in vivo animal model and monitored the healing process using 4 different imaging modalities: histology, Optical Coherence Tomography (OCT), Second Harmonic Generation (SHG), and Fourier Transform Infrared (FTIR) spectroscopy. 3 dorsal areas of 20 anesthetized Wistar rats were burned by water vapor exposure and subsequently treated either by classical surgical debridement, by laser ablation, or left without treatment. Skin burn tissues were non-invasively characterized by OCT images and biopsied for further histopathology analysis, SHG imaging and FTIR spectroscopy at 3, 5, 7 and 14 days after burn. The laser protocol was found as efficient as the classical treatment for promoting the healing process. The study concludes to the validation of femtosecond ultra-short pulses laser treatment for skinburns, with the advantage of minimizing operatory trauma.Thousands of people die every year from burn injuries. The aim of this study is to evaluate the feasibility of high intensity femtosecond lasers as an auxiliary treatment of skin burns. We used an in vivo animal model and monitored the healing process using 4 different imaging modalities: histology, Optical Coherence Tomography (OCT), Second Harmonic Generation (SHG), and Fourier Transform Infrared (FTIR) spectroscopy. 3 dorsal areas of 20 anesthetized Wistar rats were burned by water vapor exposure and subsequently treated either by classical surgical debridement, by laser ablation, or left without treatment. Skin burn tissues were non-invasively characterized by OCT images and biopsied for further histopathology analysis, SHG imaging and FTIR spectroscopy at 3, 5, 7 and 14 days after burn. The laser protocol was found as efficient as the classical treatment for promoting the healing process. The study concludes to the validation of femtosecond ultra-short pulses laser treatment for skinburns, with the advantage of minimizing operatory trauma.8315751588FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORFAPEAM - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DO AMAZONASFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORFAPEAM - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DO AMAZONAS05/51689-2; 11/51959-0573.916/2008-0; 573.913/2008-00; 312397/2013-58881.068505/2014-01Sem informaçã
Phase Transitions in Neutron Stars and Gravitational Wave Emission
We review the detectability of gravitational waves generated by oscillations
excited during a phase transition from hadronic matter to deconfined
quark-gluon matter in the core of a neutron star. Neutron star properties were
computed using a Boguta and Bodmer's based model and the MIT bag model. The
maximum energy available to excite mechanical oscillations into the star is
estimated by energy difference between the configurations with and without a
quark-gluon matter core. On basis of the planned sensitivity of present laser
interferometers (VIRGO or LIGO I) and those of the next generation (LIGO II),
the maximum volume to be proped by these experiments is determined. These
results are used as an indication of the potential detectability of neutron
stars as sources of gravitational waves. Our results indicate that the maximum
distance probed by the detectors of the first generation is well beyond M31,
whereas the second generation detectors will probably see phase transitions
events at distances two times longer, but certanly not yet attaining the Virgo
cluster.Comment: revtex4, accepted for publication in Phys. Rev.
A novel frameshift mutation of Chediak-Higashi syndrome and treatment in the accelerated phase
Transverse momentum and centrality dependence of dihadron correlations in Au+Au collisions at sqrt(s_NN)=200 GeV: Jet-quenching and the response of partonic matter
Azimuthal angle \Delta\phi correlations are presented for charged hadrons
from dijets for 0.4 < p_T < 10 GeV/c in Au+Au collisions at sqrt(s_NN) = 200
GeV. With increasing p_T, the away-side distribution evolves from a broad to a
concave shape, then to a convex shape. Comparisons to p+p data suggest that the
away-side can be divided into a partially suppressed "head" region centered at
Delta\phi ~ \pi, and an enhanced "shoulder" region centered at Delta\phi ~ \pi
+/- 1.1. The p_T spectrum for the "head" region softens toward central
collisions, consistent with the onset of jet quenching. The spectral slope for
the "shoulder" region is independent of centrality and trigger p_T, which
offers constraints on energy transport mechanisms and suggests that the
"shoulder" region contains the medium response to energetic jets.Comment: 420 authors from 58 institutions, 6 pages, 4 figures. Submitted to
Physical Review Letters. Plain text data tables for the points plotted in
figures for this and previous PHENIX publications are (or will be) publicly
available at http://www.phenix.bnl.gov/papers.htm
Azimuthal anisotropy of neutral pion production in Au+Au collisions at sqrt(s_NN) = 200 GeV: Path-length dependence of jet quenching and the role of initial geometry
We have measured the azimuthal anisotropy of pi0's for 1 < pT < 18 GeV/c for
Au+Au collisions at sqrt s_NN = 200 GeV. The observed anisotropy shows a
gradual decrease in 3 < pT < 7 - 10 GeV/c, but remains positive beyond 10
GeV/c. The magnitude of this anisotropy is under-predicted, up to at least 10
GeV/c, by current perturbative QCD (pQCD) energy-loss model calculations. An
estimate of the increase in anisotropy expected from initial-geometry
modification due to gluon saturation effects and initial-geometry fluctuations
is insufficient to account for this discrepancy. Calculations which implement a
path length dependence steeper than what is implied by current pQCD energy-loss
models, show reasonable agreement with the data.Comment: 384 authors, 6 pages text, 3 figures. Submitted to Phys. Rev. Lett.
Plain text data tables for the points plotted in figures for this and
previous PHENIX publications are (or will be) publicly available at
http://www.phenix.bnl.gov/papers.htm
Particle-species dependent modification of jet-induced correlations in Au+Au collisions at sqrt(s_NN) = 200 GeV
We report PHENIX measurements of the correlation of a trigger hadron at
intermediate transverse momentum (2.5<p_{T,trig}<4 GeV/c), with associated
mesons or baryons at lower p_{T,assoc}, in Au+Au collisions at sqrt(s_NN) = 200
GeV. The jet correlations for both baryons and mesons show similar shape
alterations as a function of centrality, characteristic of strong modification
of the away-side jet. The ratio of jet-associated baryons to mesons for this
jet increases with centrality and p_{T,assoc} and, in the most central
collisions, reaches a value similar to that for inclusive measurements. This
trend is incompatible with in-vacuum fragmentation, but could be due to
jet-like contributions from correlated soft partons which recombine upon
hadronization.Comment: 344 authors, 4 pages text, RevTeX, 4 figures. Submitted to Physical
Review Letters. Plain text data tables for the points plotted in figures for
this and previous PHENIX publications are (or will be) publicly available at
http://www.phenix.bnl.gov/papers.htm
Transverse momentum dependence of J/psi polarization at midrapidity in p+p collisions at sqrt(s)=200 GeV
We report the measurement of the transverse momentum dependence of inclusive
J/psi polarization in p+p collisions at sqrt(s)=200 GeV performed by the PHENIX
Experiment at RHIC. The polarization is studied in the helicity,
Gottfried-Jackson, and Collins-Soper frames for p_T < 5 GeV/c and |y| < 0.35.
The J/psi polarization in the helicity and Gottfried-Jackson frames is
consistent with zero for all transverse momenta, with a slight (1.8 sigma)
trend towards longitudinal polarization for transverse momenta above 2 GeV/c.
No conclusion is allowed due to the limited acceptance in the Collins-Soper
frame and the uncertainties of the current data. The results are compared to
observations for other collision systems and center of mass energies and to
different quarkonia production models.Comment: 384 authors from 62 institutions, 11 pages, 9 figures, 2 tables. v2
is expanded version submitted to Physical Review D. Plain text data tables
for the points plotted in figures are available at
http://www.phenix.bnl.gov/papers.htm
High p_T Direct Photon and pi^0 Triggered Azimuthal Jet Correlations in sqrt(s)=200 GeV p+p Collisions
Correlations of charged hadrons of 1 < pT < 10 GeV/c with high pT direct
photons and pi^ 0 mesons in the range 5 <pT < 15 GeV/c are used to study jet
fragmentation in the photon+jet and di-jet channels, respectively. The
magnitude of the partonic transverse momentum, kT, is obtained by comparing to
a model incorporating a Gaussian kT smearing. The sensitivity of the associated
charged hadron spectra to the underlying fragmentation function is tested and
the data are compared to calculations using recent global fit results. The
shape of the direct photon-associated hadron spectrum as well as its charge
asymmetry are found to be consistent with a sample dominated by quark-gluon
Compton scattering. No significant evidence of fragmentation photon correlated
production is observed within experimental uncertainties.Comment: 431 authors, 18 pages, 18 figures, 4 tables, RevTeX-4. Submitted to
Physical Review D. Plain text data tables for the points plotted in figures
for this and previous PHENIX publications are (or will be) publicly available
at http://www.phenix.bnl.gov/papers.htm
Discovery of biphenylacetamide-derived inhibitors of BACE1 using de novo structure-based molecular design
β-Secretase (BACE1), the enzyme responsible for the first and rate-limiting step in the production of amyloid-β peptides, is an attractive target for the treatment of Alzheimer’s disease. In this study, we report the application of the de novo fragment-based molecular design program SPROUT to the discovery of a series of nonpeptide BACE1 inhibitors based upon a biphenylacetamide scaffold. The binding affinity of molecules based upon this designed molecular scaffold was increased from an initial BACE1 IC50 of 323 μM to 27 μM following the synthesis of a library of optimized ligands whose structures were refined using the recently developed SPROUT-HitOpt software. Although a number of inhibitors were found to exhibit cellular toxicity, one compound in the series was found to have useful BACE1 inhibitory activity in a cellular assay with minimal cellular toxicity. This work demonstrates the power of an in silico fragment-based molecular design approach in the discovery of novel BACE1 inhibitors
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