A Novel Mouse Model of Peritoneal Dialysis: Combination of Uraemia and Long-Term Exposure to PD Fluid

Different animal models for peritoneal dialysis (PD) have been used in the past decades to develop PD fluids compatible with patient life and to identify markers of peritoneal fibrosis and inflammation. Only few of those studies have taken into account the importance of uraemia-induced alterations at both systemic and peritoneal levels. Moreover, some animal studies which have reported about PD in a uremic setting did not always entirely succeed in terms of uraemia establishment and animal survival. In the present study we induced uraemia in the recently established mouse PD exposure model in order to obtain a more clinically relevant mouse model for kidney patients. This new designed model reflected both the slight thickening of peritoneal membrane induced by uraemia and the significant extracellular matrix deposition due to daily PD fluid instillation. In addition the model offers the opportunity to perform long-term exposure to PD fluids, as it is observed in the clinical setting, and gives the advantage to knock out candidate markers for driving peritoneal inflammatory mechanisms.Marie Curie actionsPeer Reviewe

Comparison of absolute gain photometric calibration between Planck/HFI and Herschel/SPIRE at 545 and 857 GHz

We compare the absolute gain photometric calibration of the Planck/HFI and Herschel/SPIRE instruments on diffuse emission. The absolute calibration of HFI and SPIRE each relies on planet flux measurements and comparison with theoretical far-infrared emission models of planetary atmospheres. We measure the photometric cross calibration between the instruments at two overlapping bands, 545 GHz / 500 $\mu$m and 857 GHz / 350 $\mu$m. The SPIRE maps used have been processed in the Herschel Interactive Processing Environment (Version 12) and the HFI data are from the 2015 Public Data Release 2. For our study we used 15 large fields observed with SPIRE, which cover a total of about 120 deg^2. We have selected these fields carefully to provide high signal-to-noise ratio, avoid residual systematics in the SPIRE maps, and span a wide range of surface brightness. The HFI maps are bandpass-corrected to match the emission observed by the SPIRE bandpasses. The SPIRE maps are convolved to match the HFI beam and put on a common pixel grid. We measure the cross-calibration relative gain between the instruments using two methods in each field, pixel-to-pixel correlation and angular power spectrum measurements. The SPIRE / HFI relative gains are 1.047 ($\pm$ 0.0069) and 1.003 ($\pm$ 0.0080) at 545 and 857 GHz, respectively, indicating very good agreement between the instruments. These relative gains deviate from unity by much less than the uncertainty of the absolute extended emission calibration, which is about 6.4% and 9.5% for HFI and SPIRE, respectively, but the deviations are comparable to the values 1.4% and 5.5% for HFI and SPIRE if the uncertainty from models of the common calibrator can be discounted. Of the 5.5% uncertainty for SPIRE, 4% arises from the uncertainty of the effective beam solid angle, which impacts the adopted SPIRE point source to extended source unit conversion factor (Abridged)Comment: 13 pages, 10 figures; Incorporates revisions in response to referee comments; cross calibration factors unchange

Planck \u27s Dusty GEMS: VIII. Dense-gas reservoirs in the most active dusty starbursts at z ∼3

We present ALMA, NOEMA, and IRAM-30 m/EMIR observations of the high-density tracer molecules HCN, HCO+, and HNC in three of the brightest lensed dusty star-forming galaxies at z≲ 3-3.5, part of the Planck\u27s Dusty Gravitationally Enhanced subMillimetre Sources (GEMS), with the aim of probing the gas reservoirs closely associated with their exceptional levels of star formation. We obtained robust detections of ten emission lines between Jup = 4 and 6, as well as several additional upper flux limits. In PLCK_G244.8+54.9, the brightest source at z = 3.0, the HNC(5-4) line emission at 0.1″ resolution, together with other spatially-integrated line profiles, suggests comparable distributions of dense and more diffuse gas reservoirs, at least over the most strongly magnified regions. This rules out any major effect from differential lensing. This line is blended with CN(4-3) and in this source, we measure a HNC(5-4)/CN(4-3) flux ratio of 1.76 \ub10. 86. Dense-gas line profiles generally match those of mid-J CO lines, except in PLCK_G145.2+50.8, which also has dense-gas line fluxes that are relatively lower, perhaps due to fewer dense cores and more segregated dense and diffuse gas phases in this source. The HCO+/HCN 1 and HNC/HCN ∼ 1 line ratios in our sample are similar to those of nearby ultraluminous infrared galaxies (ULIRGs) and consistent with photon-dominated regions without any indication of important mechanical heating or active galactic nuclei feedback. We characterize the dense-gas excitation in PLCK_G244.8+54.9 using radiative transfer models assuming pure collisional excitation and find that mid-J HCN, HCO+, and HNC lines arise from a high-density phase with an H2 density of n ∼ 105-106 cm-3, although important degeneracies hinder a determination of the exact conditions. The three GEMS are consistent with extrapolations of dense-gas star-formation laws derived in the nearby Universe, adding further evidence that the extreme star-formation rates observed in the most active galaxies at z ∼ 3 are a consequence of their important dense-gas contents. The dense-gas-mass fractions traced by HCN/[CI] and HCO+/[CI] line ratios are elevated, but not exceptional as compared to other lensed dusty star-forming galaxies at z > 2, and they fall near the upper envelope of local ULIRGs. Despite the higher overall gas fractions and local gas-mass surface densities observed at high redshift, the dense-gas budget of rapidly star-forming galaxies seems to have evolved little between z ∼ 3 and z ∼ 0. Our results favor constant dense-gas depletion times in these populations, which is in agreement with theoretical models of star formation

Enrichment and characterization of dendritic cells from human bronchoalveolar lavages

In the present study about 0.3% to 1.6% of human bronchoalveolar lavage (BAL) cells were identified as typical dendritic cells (DC), having an irregular outline, lobulated nucleus, and clear distinguishable acid phosphatase activity or EBM11 (anti-CD68) reactivity in a spot near the nucleus. After DC enrichment, using transient adherence to plastic, FcR-panning, and a density metrizamide gradient, a population containing 7-8% typical DC was obtained. This DC-enriched low density fraction, containing the highest percentages of DC, very strongly induced T cell proliferation in an allogeneic mixed leucocyte reaction (MLR), which was significantly higher than that induced by other partly (un)fractionated BAL cells. These data indicate that DC seem to be the major accessory cells in the BAL fluid, and therefore may be important in the regulation of T cell immune responses in the lung

Gas and dust in a submillimeter galaxy at z = 4.24 from the Herschel ATLAS

We report ground-based follow-up observations of the exceptional source, ID141, one the brightest sources detected so far in the H-ATLAS cosmological survey. ID141 was observed using the IRAM 30-meter telescope and Plateau de Bure interferometer (PdBI), the Submillimeter Array (SMA) and the Atacama Pathfinder Experiment (APEX) submillimeter telescope to measure the dust continuum and emission lines of the main isotope of carbon monoxide and carbon ([C I] and [C II]). The detection of strong CO emission lines with the PdBI confirms that ID141 is at high redshift (z=4.243 +/- 0.001). The strength of the continuum and emission lines suggests that ID141 is gravitationally lensed. The width (Delta V (FWHM) ~ 800 km/s}) and asymmetric profiles of the CO and carbon lines indicate orbital motion in a disc or a merger. The properties derived for ID141 are compatible with a ultraluminous (L_FIR ~ 8.5 +/- 0.3 x 10^13/mu_L Lsun, where mu_L is the amplification factor, dense (n ~ 10^4 cm^-3) and warm (T_kin ~ 40K) starburst galaxy, with an estimated star-formation rate of (0.7 to 1.7) x 10^4/mu_L Msun/yr. The carbon emission lines indicate a dense (n ~ 10^4 cm^-3) Photo-Dominated Region, illuminated by a far-UV radiation field a few thousand times more intense than that in our Galaxy. In conclusion, the physical properties of the high-z galaxy, ID141, are remarkably similar to those of local ultraluminous infrared galaxies.Comment: To appear in Ap

Far-Infrared Properties of Spitzer-selected Luminous Starbursts

We present SHARC-2 350 micron data on 20 luminous z ~ 2 starbursts with S(1.2mm) > 2 mJy from the Spitzer-selected samples of Lonsdale et al. and Fiolet et al. All the sources were detected, with S(350um) > 25 mJy for 18 of them. With the data, we determine precise dust temperatures and luminosities for these galaxies using both single-temperature fits and models with power-law mass--temperature distributions. We derive appropriate formulae to use when optical depths are non-negligible. Our models provide an excellent fit to the 6um--2mm measurements of local starbursts. We find characteristic single-component temperatures T1 ~ 35.5+-2.2 K and integrated infrared (IR) luminosities around 10^(12.9+-0.1) Lsun for the SWIRE-selected sources. Molecular gas masses are estimated at 4 x 10^(10) Msun, assuming kappa(850um)=0.15 m^2/kg and a submillimeter-selected galaxy (SMG)-like gas-to-dust mass ratio. The best-fit models imply >~2 kpc emission scales. We also note a tight correlation between rest-frame 1.4 GHz radio and IR luminosities confirming star formation as the predominant power source. The far-IR properties of our sample are indistinguishable from the purely submillimeter-selected populations from current surveys. We therefore conclude that our original selection criteria, based on mid-IR colors and 24 um flux densities, provides an effective means for the study of SMGs at z ~ 1.5--2.5.Comment: 13 pages, 4 figures, edited to match published version in ApJ 717, 29-39 (2010

Planck's Dusty GEMS: Gravitationally lensed high-redshift galaxies discovered with the Planck survey

We present an analysis of 11 bright far-IR/submm sources discovered through a combination of the Planck survey and follow-up Herschel-SPIRE imaging. Each source has a redshift z=2.2-3.6 obtained through a blind redshift search with EMIR at the IRAM 30-m telescope. Interferometry obtained at IRAM and the SMA, and optical/near-infrared imaging obtained at the CFHT and the VLT reveal morphologies consistent with strongly gravitationally lensed sources. Additional photometry was obtained with JCMT/SCUBA-2 and IRAM/GISMO at 850 um and 2 mm, respectively. All objects are bright, isolated point sources in the 18 arcsec beam of SPIRE at 250 um, with spectral energy distributions peaking either near the 350 um or the 500 um bands of SPIRE, and with apparent far-infrared luminosities of up to 3x10^14 L_sun. Their morphologies and sizes, CO line widths and luminosities, dust temperatures, and far-infrared luminosities provide additional empirical evidence that these are strongly gravitationally lensed high-redshift galaxies. We discuss their dust masses and temperatures, and use additional WISE 22-um photometry and template fitting to rule out a significant contribution of AGN heating to the total infrared luminosity. Six sources are detected in FIRST at 1.4 GHz. Four have flux densities brighter than expected from the local far-infrared-radio correlation, but in the range previously found for high-z submm galaxies, one has a deficit of FIR emission, and 6 are consistent with the local correlation. The global dust-to-gas ratios and star-formation efficiencies of our sources are predominantly in the range expected from massive, metal-rich, intense, high-redshift starbursts. An extensive multi-wavelength follow-up programme is being carried out to further characterize these sources and the intense star-formation within them.Comment: A&A accepte

Recruitment, augmentation and apoptosis of rat osteoclasts in 1,25-(OH)2D3 response to short-term treatment with 1,25-dihydroxyvitamin D3in vivo

Background Although much is known about the regulation of osteoclast (OC) formation and activity, little is known about OC senescence. In particular, the fate of of OC seen after 1,25-(OH)2D3 administration in vivo is unclear. There is evidence that the normal fate of OC is to undergo apoptosis (programmed cell death). We have investigated the effect of short-term application of high dose 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on OC apoptosis in an experimental rat model. Methods OC recruitment, augmentation and apoptosis was visualised and quantitated by staining histochemically for tartrate resistant acid phosphatase (TRAP), double staining for TRAP/ED1 or TRAP/DAPI, in situ DNA fragmentation end labelling and histomorphometric analysis. Results Short-term treatment with high-dose 1,25-(OH)2D3 increased the recruitment of OC precursors in the bone marrow resulting in a short-lived increase in OC numbers. This was rapidly followed by an increase in the number of apoptotic OC and their subsequent removal. The response of OC to 1,25-(OH)2D3 treatment was dose and site dependent; higher doses producing stronger, more rapid responses and the response in the tibiae being consistently stronger and more rapid than in the vertebrae. Conclusions This study demonstrates that (1) after recruitment, OC are removed from the resorption site by apoptosis (2) the combined use of TRAP and ED1 can be used to identify OC and their precursors in vivo (3) double staining for TRAP and DAPI or in situ DNA fragmentation end labelling can be used to identify apoptotic OC in vivo

Four quasars above redshift 6 discovered by the Canada-France High-z Quasar Survey

The Canada-France High-z Quasar Survey (CFHQS) is an optical survey designed to locate quasars during the epoch of reionization. In this paper we present the discovery of the first four CFHQS quasars at redshift greater than 6, including the most distant known quasar, CFHQS J2329-0301 at z=6.43. We describe the observational method used to identify the quasars and present optical, infrared, and millimeter photometry and optical and near-infrared spectroscopy. We investigate the dust properties of these quasars finding an unusual dust extinction curve for one quasar and a high far-infrared luminosity due to dust emission for another. The mean millimeter continuum flux for CFHQS quasars is substantially lower than that for SDSS quasars at the same redshift, likely due to a correlation with quasar UV luminosity. For two quasars with sufficiently high signal-to-noise optical spectra, we use the spectra to investigate the ionization state of hydrogen at z>5. For CFHQS J1509-1749 at z=6.12, we find significant evolution (beyond a simple extrapolation of lower redshift data) in the Gunn-Peterson optical depth at z>5.4. The line-of-sight to this quasar has one of the highest known optical depths at z~5.8. An analysis of the sizes of the highly-ionized near-zones in the spectra of two quasars at z=6.12 and z=6.43 suggest the IGM surrounding these quasars was substantially ionized before these quasars turned on. Together, these observations point towards an extended reionization process, but we caution that cosmic variance is still a major limitation in z>6 quasar observations.Comment: 15 pages, 9 figures, AJ, in press, minor changes to previous versio