202 research outputs found
Book reviews
Nanoencapsulation technologies for the food and nutraceutical
industries
S.M. JAFARI (Ed.)
Academic Press, Elsevier Science Publishing Co Inc., 2016, 125 London Wall, London EC2Y 5AS, United
Kingdom, ISBN 978-0-12-809436-5, 610 pages | Food safety in the 21st century: Public health perspective (1st ed.)
R. K. GUPTA, B. P. DUDEJA and A. S. MINHAS (Eds)
Academic Press is an imprint of Elsevier, 125 London Wall, London EC2Y 5AS, United Kingdom
ISBN: 978-0128017739, 624 pages | Molecular methods for the detection and characterization of foodborne
and environmental pathogens
R.K. GUPTA, B. P. DUDEJA and A. S. MINHAS (Eds)
DEStech Publications, Inc. 439 North Duke Street, Lancaster, Pennsylvania 17602 U.S.A.
ISBN No. 978-1-60595-079-2, 156 pages | Foodborne diseases
3rd edition
C.E.R. DODD, T. ALDSWORTH, R.A. STEIN, D.O. CLIVER and H.P. RIEMANN (Eds)
Academic Press is an imprint of Elsevier, London, UK, San Diego, USA, Cambridge, USA, Oxford, UK, 2016,
ISBN: 978-0-12-385007-2 (print), 576 pages | The vitamins: Fundamental aspects in nutrition and health
5th edition
G.F. COMBS and J.P. MCCLUNG (Eds)
Academic Press is an imprint of Elsevier, London, UK, San Diego, USA, Cambridge, USA, Oxford, UK, 2016,
ISBN: 978-0-12-802965-7, 628 page
Plant polysaccharide degrading enzyme system of Thermobifida cellulosilytica TB100<sup>T</sup> revealed by de novo genome project data
Thermobifidas are thermophilic, aerobic, lignocellulose decomposing actinomycetes. The Thermobifida genus includes four species: T. fusca, T. alba, T. cellulosilytica, and T. halotolerans. T. fusca YX is the far best characterized strain of this taxon and several cellulases and hemicellulases have been cloned from it for industrial purposes targeting paper industry, biofuel, and feed applications. Unfortunately, sequence data of such enzymes are almost exclusively restricted to this single species; however, we demonstrated earlier by zymography that other T. alba and T. cellulosilytica strains encode the same enzyme sets. Recently, the advances in whole genome sequencing by the use of next generation genomics platforms accelerated the selection process of valuable hydrolases from uncharacterized bacterial species for cloning purposes. For this purpose T. cellulosilytica TB100T type strain was chosen for de novo genome sequencing. We have assembled the genome of T. cellulosilytica strain TB100T into 168 contigs and 19 scaffolds, with reference length of 4 327 869 bps, 3 589 putative coding sequences, 53 tRNAs, and 4 rRNAs. The analysis of the annotated genome revealed the existence of 27 putative hydrolases belonging to 14 different glycoside hydrolase (GH) families. The investigation of identified, cloned, and heterologously multiple cellulases, mannanases, xylanases, and amylases may result in industrial applications beside gaining useful basic research related information
Complex renal cysts (Bosniak â„IIF):Interobserver agreement, Progression and Malignancy rates
Reverse Hall-Petch effect in ultra nanocrystalline diamond
We present atomistic simulations for the mechanical response of ultra
nanocrystalline diamond, a polycrystalline form of diamond with grain diameters
of the order of a few nm. We consider fully three-dimensional model structures,
having several grains of random sizes and orientations, and employ
state-of-the-art Monte Carlo simulations. We calculate structural properties,
elastic constants and the hardness of the material; our results compare well
with experimental observations for this material. Moreover, we verify that this
material becomes softer at small grain sizes, in analogy to the observed
reversal of the Hall-Petch effect in various nanocrystalline metals. The effect
is attributed to the large concentration of grain boundary atoms at smaller
grain sizes. Our analysis yields scaling relations for the elastic constants as
a function of the average grain size.Comment: Proceedings of the IUTAM Symposium on Modelling Nanomaterials and
Nanosystems, Aalborg, Denmark, May 19-22 2008; to be published in the IUTAM
Bookseries by Springe
EuroGuiDerm Guideline on the systemic treatment of Psoriasis vulgaris â : Part 1: treatment and monitoring recommendations
Peer reviewe
EuroGuiDerm Guideline on the systemic treatment of Psoriasis vulgaris - Part 2 : specific clinical and comorbid situations
This evidence- and consensus-based guideline on the treatment of psoriasis vulgaris was developed following the EuroGuiDerm Guideline and Consensus Statement Development Manual. The second part of the guideline provides guidance for specific clinical and comorbid situations such as treating psoriasis vulgaris patient with concomitant psoriatic arthritis, concomitant inflammatory bowel disease, a history of malignancies or a history of depression or suicidal ideation. It further holds recommendations for concomitant diabetes, viral hepatitis, disease affecting the heart or the kidneys as well as concomitant neurological disease. Advice on how to screen for tuberculosis and recommendations on how to manage patients with a positive tuberculosis test result are given. It further covers treatment for pregnant women or patients with a wish for a child in the near future. Information on vaccination, immunogenicity and systemic treatment during the COVID-19 pandemic is also provided.Peer reviewe
Recurrent Scedosporium apiospermum mycetoma successfully treated by surgical excision and terbinafine treatment: a case report and review of the literature
Background:
Scedosporium apiospermum
is an emerging opportunistic filamentous fungus, which is notorious for its
high levels of antifungal
âresistance. It is able to cause localized cutaneous or subcutaneous infections in both immuâ
nocompromised and immunocompetent persons, pulmonary infections in patients with predisposing pulmonary
diseases and invasive mycoses in immunocompromised patients. Subcutaneous infections caused by this fungus
frequently show chronic mycetomatous manifestation.
Case report:
We report the case of a 70
âyear
âold immunocompromised man, who developed a fungal mycetomaâ
tous infection on his right leg. There was no history of trauma; the aetiological agent was identified by microscopic
examination and ITS sequencing. This is the second reported case of
S. apiospermum
subcutaneous infections in
Hungary, which was successfully treated by surgical excision and terbinafine treatment. After 7
months, the patient
remained asymptomatic. Considering the antifungal susceptibility and increasing incidence of the fungus,
Sce
-
dosporium
related subcutaneous infections reported in the past quarter of century in European countries were also
reviewed.
Conclusions:
Corticosteroid treatment represents a serious risk factor of
S. apiospermum
infections, especially if the
patient get in touch with manure
âenriched or polluted soil or water. Such infections have emerged several times in
European countries in the past decades. The presented data suggest that besides the commonly applied voriconaâ
zole, terbinafine may be an alternative for the therapy of mycetomatous
Scedosporium
infections
Expression of the chemokine receptor CCR5 in psoriasis and results of a randomized placebo controlled trial with a CCR5 inhibitor
Several reports have indicated that the chemokine receptor CCR5 and its ligands, especially CCL5 (formerly known as RANTES), may play a role in the pathogenesis of psoriasis. The purpose of this investigation was to examine the expression of CCR5 and its ligands in chronic plaque psoriasis and to evaluate the clinical and immunohistochemical effect of a CCR5 receptor inhibitor. Immunohistochemical analysis showed low but significant increased total numbers of CCR5 positive cells in epidermis and dermis of lesional skin in comparison to non-lesional skin. However, relative expression of CCR5 proportional to the cells observed revealed that the difference between lesional and non-lesional skin was only statistically significant in the epidermis for CD3 positive cells and in the dermis for CD68 positive cells. Quantification of mRNA by reverse transcriptase-polymerase chain reaction only showed an increased expression of CCL5 (RANTES) in lesional skin. A randomized placebo-controlled clinical trial in 32 psoriasis patients revealed no significant clinical effect and no changes at the immunohistochemical level comparing patients treated with placebo or a CCR5 inhibitor SCH351125. We conclude that although CCR5 expression is increased in psoriatic lesions, this receptor does not play a crucial role in the pathogenesis of psoriasis
In Vitro Dedifferentiation of Melanocytes from Adult Epidermis
In previous work we described a novel culture technique using a cholera toxin and PMA-free medium (Mel-mix) for obtaining pure melanocyte cultures from human adult epidermis. In Mel-mix medium the cultured melanocytes are bipolar, unpigmented and highly proliferative. Further characterization of the cultured melanocytes revealed the disappearance of c-Kit and TRP-1 and induction of nestin expression, indicating that melanocytes dedifferentiated in this in vitro culture. Cholera toxin and PMA were able to induce c-Kit and TRP-1 protein expressions in the cells, reversing dedifferentiation. TRP-1 mRNA expression was induced in dedifferentiated melanocytes by UV-B irradiated keratinocyte supernatants, however direct UV-B irradiation of the cells resulted in further decrease of TRP-1 mRNA expression. These dedifferentiated, easily accessible cultured melanocytes provide a good model for studying melanocyte differentiation and possibly transdifferentiation. Because melanocytes in Mel-mix medium can be cultured with human serum as the only supplement, this culture system is also suitable for autologous cell transplantation
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