Interleukin-6 Gene Polymorphisms Influencing in hematological indices from sickle cell Anemia Patients

The homozygous hemoglobin SS is characterized Sickle Cell Anemia (SCA), altering the original structure of erythrocytes to a sickle shape.  The hemoglobinopathies encompass all genetic diseases of hemoglobin and the SCA is the one that presents the greatest clinical manifestations variability and also the most severe ones, causing chronic hemolysis, vaso-occlusive crises and severe anemia in patients. The present study aimed to investigate the role of rs2069832, rs2069835, rs2069840, rs2069845 and rs2069849 polymorphisms in the Interleukin-6 gene in the hematological values of SCA patients treated at Fundação HEMOAM, Manaus, AM. The inclusion of patients was carried out through outpatient care at HEMOAM. Genomic DNA was extracted using the QIAamp DNA Mini Kit (Qiagen) and molecular analyzes by TaqMan® probes on Applied Biosystems QuantStudio 6 Flex Real-Time PCR System. A total of 277 SCA patients were included in this study, having the female gender having a minimally higher frequency (55.3%). The mean age at diagnosis was approximately three years old, with brown race being the most predominant (77.6%). The rs2069832_AA and rs2069845_AA genotypes showed high values for red blood cell, hemoglobin and hematocrit indices, by having an important role as a protective factor for hemolysis in these patients. While the rs2069835_CC genotype showed decreased values for the same hematimetric indices, demonstrating to be a potential risk factor for increased hemolysis. No significant correlation in hematimetric indices was observed for the rs2069840 and rs2069849 genotypes. There are few studies correlating the genetic variants of the IL-6 gene in SCA in the state of Amazonas, however, it is known that IL-6 is involved in cell proliferation and greater response to inflammatory cytokines, and may modulate the clinical response in these patients, such as chronic hemolysis, vaso-occlusion and infections. Our work demonstrated associations of risk and protective IL-6 genotypes for possible hemolysis in patients with sickle cell anemia. We understand that an investigation with a larger number of patients would be recommended to elucidate the roles of the studied polymorphisms in sickle cell anemia. In addition, elucidating the role of Il-6 in sickle cell anemia may lead to the development of new strategies and therapies to prevent the systemic effects of excessive cytokine production and, consequently, reduce the severity of crises in these patients, providing better prognosis, clinical follow-up and welfare

Guidelines for surgery of aortic diseases from Brazilian Society of Cardiovascular Surgery

Sociedade Brasileira de Cirurgia CardiovascularUniversidade Federal do Rio Grande do SulPUCRS Hospital São LucasFamerp Pós-GraduaçãoUnicampUNIFESPSBCCVUFRGS Faculdade de Medicina-Cirurgia CardiovascularEPM, UNIFESPUNIFESPEPMSciEL

COOPERAÇÃO, ESTRATIFICAÇÃO E PERFIL DA PESQUISA EM ESTRATÉGIA NO BRASIL

The documentary analysis of publications permits to identify scientific production standards associated with characteristics and tendencies expressed in a certain area of knowledge. Considering the area of Strategy in Organizations and its thematic axes, this study analyzed 765 articles published between 2001 and 2006, in the proceedings of EnAnpad’s and 3Es in order to evaluate quantitatively the research design mostly used throughout the period. Moreover, tendencies expressed in terms of stratification standards and cooperation in the area were also investigated. The data were treated in a descriptive-quantitative way, through statistic procedures and multivariate technique of analysis of multiple correspondence. The findings indicated a predominance of a scientific production focused on empirical articles, mainly case studies and surveys. Additionally, the results revealed the growth of the area concerning the number of articles, authors and institutions, accompanied by greater cooperation in scientific production and changes regarding the regional and institutional stratification standards. A análise documental de publicações permite identificar características e tendências expressas em determinada área do conhecimento científico. Tendo em vista a área de Estratégia em Organizações, este estudo analisou 765 artigos, publicados entre 2001 e 2006, nos anais dos EnAnpad e 3Es, objetivando avaliar quantitativamente os delineamentos de pesquisa utilizados e analisar tendências acerca dos padrões de estratificação e cooperação na área. Por meio de procedimentos estatísticos e da técnica multivariada de análise de correspondência múltipla, verificou-se a predominância de uma produção científica voltada para artigos empíricos apoiados primordialmente em estudos de caso e análises quantitativas tipo surveys. Observou-se também que, ao longo do período investigado, houve crescimento da área no que concerne ao número de artigos, autores e instituições, com ocorrência de maior cooperação na produção acadêmica, além de alterações nos padrões de estratificação regional e institucional. 

Prevalence of peripheral arterial disease in patients with heart failure with preserved ejection fraction

OBJECTIVES: To describe the prevalence of the reduced ankle-brachial index (ABI) in patients with heart failure (HF) with preserved ejection fraction (HFpEF) attended at a HF clinic in the metropolitan region of Porto Alegre, and to compar the patients to those with reduced ejection fraction (HFrEF). METHODS: A descriptive observational study, included patients referred to the heart failure clinic in HU-Ulbra with HFpEF or HFrEF and diastolic dysfunction, and measurements of ABIs using vascular Doppler equipment were performed in both groups. RESULTS: The sample consisted of 106 patients with HF, 53.9% of the patients had HFpEF, and 19.4% had a diagnosis of peripheral arterial disease (PAD) (ABI less than 0.9). PAD was identified in 24.1% of the patients with HFpEF, while15.8% of patients in the HFrEF group were diagnosed with PAD. CONCLUSION: Our results did not identify a significantly different prevalence of altered and compatible PAD values in patients with HFpEF. However, we showed a prevalence of 19.4%, a high value if we consider similar populations

Condições meteorológicas e tipo de solo na composição da uva 'Cabernet Sauvignon'

O objetivo deste trabalho foi avaliar os efeitos das condições meteorológicas e do tipo de solo sobre características físico‑químicas e compostos fenólicos da uva 'Cabernet Sauvignon' (Vitis vinifera). O experimento foi realizado em vinhedo implantado em 2003, enxertado sobre o porta‑enxerto 'Paulsen 1103' e conduzido no sistema espaldeira. No vinhedo, foram selecionados dois solos: Cambissolo Háplico e Cambissolo Húmico. O efeito das condições meteorológicas (precipitação e temperatura mínima e máxima do ar) foi avaliado nas safras 2008/2009, 2009/2010 e 2010/2011. Foram determinados os atributos físicos e químicos dos solos, os teores de sólidos solúveis, a acidez titulável e o pH do mosto, bem como o índice de polifenóis totais e dos teores de antocianinas e de taninos da uva. Os fatores solo e as condições meteorológicas (safras) foram arranjados em esquema fatorial 2x3. Com exceção do teor de polifenóis totais, as condições meteorológicas e o tipo de solo afetam as características físico‑químicas da uva 'Cabernet Sauvignon', comefeito mais pronunciado das condições meteorológicas do que do tipo de solo. Menores precipitações e maiores amplitudes térmicas favorecem o acúmulo de sólidos solúveis na uva 'Cabernet Sauvignon'. Maiores precipitações favorecem o aumento da acidez do mosto. The objective of this work was to evaluate the effects of weather and soil type on the physicochemical characteristics and phenolic compounds of the 'Cabernet Sauvignon' grape (Vitis vinifera). The experiment was carried out in a vineyard established in 2003, grafted onto 'Paulsen 1103', and conducted in the cordon system. Two soils were selected in the vineyard: Typic Dystrudepts and Pachic Humudepts. The effect of weather (rainfall, and minimum and maximum temperature) was evaluated in the seasons 2008/2009, 2009/2010, and 2010/2011. Soil physical and chemical properties, soluble solids, titratable acidity and pH of the wort were determined, as well as the index of total polyphenols, and anthocyanin and tannin contents of the grape. The factors soil and meteorological conditions (crop seasons) were arranged in a 2x3 factorial. Except for the total polyphenol content, weather and soil type affect the physicochemical characteristics of 'Cabernet Sauvignon'. The effect of weather is more pronounced than the effect of soil type. Lower rainfall and higher temperature ranges favor the accumulation of soluble solids in the 'Cabernet Sauvignon' grape. Higher rainfall favors the increase of wine acidity

Guidelines for surgery of aortic diseases from the Brazilian Society of Cardiovascular Surgery: updated in 2009

PUCRS Hospital São LucasFamerpUnicampUNIFESPUFRGS HCPA Faculdade de MedicinaUNIOESTE HUOPFAG Curso de MedicinaUNIFESP-EPMUNIFESP, EPMSciEL

CONEXÕES EPIDEMIOLÓGICAS ENTRE TELAS E MOVIMENTO, COMPORTAMENTO SEDENTÁRIO, (IN)ATIVIDADE FÍSICA E HÁBITOS DE SAÚDE

This study analyzed physical activity patterns, sedentary behaviors and exposure to screens in students from four school units in Rio de Janeiro. The objective was to provide insights for the development of health promotion strategies through physical activities and sports (AFES). Using the IPAQ questionnaire, data were collected from 1668 students, with an average age of approximately 13.8 years from all grades of primary and secondary education. Statistical tests were used for analysis. The results indicated that students walked, on average, more than 10 minutes per day on 4 days (male) and 3 days (female), with 75 minutes (male) and 69 minutes (female) of daily walking, respectively. The average of moderate activities was 91 minutes per day, while vigorous activities totaled, on average, 126 minutes (male) and 71 minutes (female). Significant sex differences were observed in daily walking, moderate and vigorous activities, and weekly inactivity time. Variations were also identified between school units, indicating differences in the application of AFES. The positive and significant correlation between the total number of days and the time of vigorous activities (ρ = 0.65; p ≤ 0.05) highlights the importance of different strategies. With regard to inactivity and exposure to screens, the average time during the week was 574 minutes, while on weekends it was 510 minutes. These results contribute to direct health promotion policies, emphasizing the need for personalized approaches for students in Rio de Janeiro.Este estudio analizó patrones de actividad física, conductas sedentarias y exposición a pantallas en estudiantes de cuatro unidades escolares de Río de Janeiro. El objetivo fue proporcionar conocimientos para el desarrollo de estrategias de promoción de la salud a través de actividades físicas y deportivas (AFES). Utilizando el cuestionario IPAQ, se recogieron datos de 1668 estudiantes, con una edad promedio de aproximadamente 13,8 años, de todos los grados de educación primaria y secundaria. Se utilizaron pruebas estadísticas para el análisis. Los resultados indicaron que los estudiantes caminaron, en promedio, más de 10 minutos por día durante 4 días (hombres) y 3 días (mujeres), con 75 minutos (hombres) y 69 minutos (mujeres) de caminata diaria, respectivamente. El promedio de actividades moderadas fue de 91 minutos por día, mientras que las actividades vigorosas sumaron, en promedio, 126 minutos (hombres) y 71 minutos (mujeres). Se observaron diferencias de sexo significativas en la caminata diaria, las actividades moderadas y vigorosas y el tiempo de inactividad semanal. También se identificaron variaciones entre unidades escolares, lo que indica diferencias en la aplicación de AFES. La correlación positiva y significativa entre el número total de días y el tiempo de actividades vigorosas (ρ = 0,65; p ≤ 0,05) resalta la importancia de diferentes estrategias. En cuanto a la inactividad y exposición a pantallas, el tiempo medio entre semana fue de 574 minutos, mientras que los fines de semana fue de 510 minutos. Estos resultados contribuyen a políticas directas de promoción de la salud, enfatizando la necesidad de enfoques personalizados para los estudiantes de Río de Janeiro.Este estudo analisou os padrões de atividade física, comportamentos sedentários e exposição a telas em estudantes de quatro unidades escolares no Rio de Janeiro. O objetivo foi fornecer insights para o desenvolvimento de estratégias de promoção da saúde através de atividades físicas e esportes (AFES). Utilizando o questionário IPAQ, foram coletados dados de 1668 estudantes, com idade média de aproximadamente 13.8 anos de todas as séries do ensino fundamental e médio. Testes estatísticos foram empregados para análise. Os resultados indicaram que os estudantes caminharam, em média, mais de 10 minutos por dia em 4 dias (masculino) e 3 dias (feminino), com 75 minutos (masculino) e 69 minutos (feminino) de caminhada diária, respectivamente. A média de atividades moderadas foi de 91 minutos por dia, enquanto as atividades vigorosas totalizaram, em média, 126 minutos (masculino) e 71 minutos (feminino). Diferenças significativas entre sexos foram observadas na caminhada diária, atividades moderadas e vigorosas, além do tempo de inatividade semanal. Variações também foram identificadas entre as unidades escolares, indicando diferenças na aplicação das AFES. A correlação positiva e significativa entre o total de dias e o tempo de atividades vigorosas (ρ = 0.65; p ≤ 0.05) destaca a importância de estratégias diferenciadas. No que diz respeito à inatividade e exposição a telas, a média de tempo durante a semana foi de 574 minutos, enquanto nos finais de semana foi de 510 minutos. Esses resultados contribuem para direcionar políticas de promoção da saúde, enfatizando a necessidade de abordagens personalizadas para estudantes no Rio de Janeiro

Self-reported oral health of a quilombola population in the semi-arid region of Piauí

The needy epidemiology of oral health in quilombola populations has been demonstrated by different researches, with the intention that studies be carried in order to identify and determine situations that are liable to be resolved through the help of social and health policies. Objective. To know the perception of oral health regarding oral discomfort and to compare it with general health in a quilombola population in the semi-arid region of Piauí. Methodology. Cross-sectional, descriptive and analytical study. From the adult and elderly population, a sample was calculated considering a margin of error of 5% and 90% of reliability, obtaining a total of 120 people as a minimum sample. Results. Inhabitants of the Canabrava, Tronco and Custaneiras quilombola communities participated - whose age ranged from 21 to 81 years old, SD±16.0. There is a predominance of: married (67.5%), female (60.8%) small farmers (90%), non-retired (71.7%), income of up to 01 (one) minimum wage (58.3%), and incomplete primary school (42.5%). Oral discomfort had a higher percentage due to the variable -Feels dry mouth (30.8%). There was a significant association (p= 0.031) between the perception of general health (excellent, reasonable and poor) and having oral health problems. Conclusion. The association between the perception of general health and having oral health problems reinforces the fact that the poor condition of oral healthinfo:eu-repo/semantics/publishedVersio

Prevalence and health promotion actions related to hyposalivation and mouth discomfort in a nursing homesfor elders

Objectiveto verify the prevalence of hyposalivation inan institutionalized elder population and its relationship with oral discomfort. A cross-sectional study was conducted with older people living in a Nursing Homein Fortaleza-Brazil. Data collection occurred through the application of sociodemographic questionnaire; sialometry examination, test for evaluating the salivary flow speed; and by a subjective quantification of dry mouth intensity (Visual Analog Scale). Results: sixty-five older people aged between 61 and 91 years (mean74.5±8.0) were evaluated, 30(46.2%) were women and 35(53.8%) men. The majority had low monthly income (55;84.6%) and low schooling (44;67.7%). The mean salivary flow was 0.4 ±0.2, withvery low flow (0.1-0.3 ml/min) being more prevalent; females presented lower salivary flow (p=0.033) than males; there was an association between salivary flow and dry mouth sensation (p<0.001) and halitosis (p<0.001). The correlation between sialometry and VAS showed that the lower the salivary flow, the greater the perception of dry mouth sensation. Health promotion actions and treatment (proper hydration; tooth brushing; saliva stimulants) can be performed in order to minimize dry mouth/ hyposalivation impact on elders’ quality of life. Conclusion: Low salivary flow was prevalent in the study subjects. It interferes in oral discomfort and causes dry mouth sensation.info:eu-repo/semantics/publishedVersio