生物电化学电解池的设计及应用

根据生物大分子的生理特性，设计了三种不同的电化学电解池，分别用于研究猪脾铁蛋白、细菌铁蛋白的电化学特性，以及电诱导棕色固氮菌表达新生理特性。说明了电解池的设计特点，给出了电解池示意图。实验结果表明，猪脾铁蛋白及细菌铁蛋白能直接从铂电极上获得还原电子，是非电极惰性蛋白质。棕色固氮菌能在电调控下被诱导成抗氨阻遏的菌株，合成固氮酶。这三种电解他也适合用于分析各种酶、蛋白质及细胞的电化学特性，并具有快速、准确、方便等特点

Degradation performance of the digital coralline hydroxyapatite artificial bone scaffold in vitro

背景:前期实验成功制备了数字化珊瑚羟基磷灰石人工骨支架材料,并已证实其具有良好的理化性能和生物相容性。目的:评价数字化珊瑚羟基磷灰石人工骨的体外降解性能。方法:将珊瑚羟基磷灰石与左旋-聚乳酸分别以3∶1和4∶1的质量比混合,制备数字化珊瑚羟基磷灰石人工骨支架材料试件。将两种数字化人工骨支架材料、珊瑚羟基磷灰石及左旋-聚乳酸分别置于初始p H值为7.4的50 m L模拟体液中,在37℃恒温箱中降解16周,动态观察溶液p H值、钙及磷离子浓度,以及材料降解率、抗压强度及微观结构变化。结果与结论:降解16周时,两种数字化人工骨组的p H值维持在7.34-7.36,高于左旋-聚乳酸组(P3∶1数字化人工骨组>左旋-聚乳酸组>4∶1数字化人工骨组;两种数字化人工骨微孔结构增多,孔隙率升高,孔径增大,说明数字化珊瑚羟基磷灰石人工骨支架具有良好的降解性能。BACKGROUND: We have successfully prepared the digital coralline hydroxyapatite artificial bone scaffold in previous experiments, and it has good physicochemical properties and biocompatibility. OBJECTIVE: To evaluate the in vitro degradation performance of the digital coralline hydroxyapatite artificial bone. METHODS: We used the mixtures of coralline hydroxyapatite and L-polylactic acid at the mass ratio of 3:1 and 4:1 as raw materials to prepare the digital coralline hydroxyapatite artificial bone scafflold specimens, and then they were immersed in the 50 m L stimulated body fluid with the initial p H value of 7.4 in an incubator at 37 ℃ for degradation. After 16 weeks of degradation, the p H value, calcium and phosphate ion concentration, degradation rate, compressive strength and changes of microstructure were dynamically observed. RESULTS AND CONCLUSION: At the 16 th weeks of degradation, the p H values in the two kinds of digital artificial bone groups maintained at 7.34-7.36, which were higher than that in the L-polylactic acid group(P 3:1 digital artificial bone group > L-polylactic acid group > 4:1 digital artificial bone group. The cellular structure, porosity and pore size in the two kinds of digital artificial bone groups were all increased. These results show that the prepared digital coralline hydroxyapatite artificial bone scaffold has good degradation propertyies.福建省教育厅科技项目(JA12416);; 福建省自然科学基金青年创新项目(2013D013);; 漳州职业技术学院院级科研项目(ZZY1204)~

Preparation of Chitosan Magnetic Microspheres

用共沉淀法制备Fe3O4磁性纳米粒子,并对其用油酸进行表面改性,继而采用沉淀聚合法制备壳聚糖磁性微球。考察了沉淀剂浓度、乳化剂种类、Fe3O4的改性等条件对制备微球的影响。应用扫描电镜、红外谱图、接触角仪、粒径分析仪及磁铁吸附对壳聚糖磁性微球的形态与特性进行了表征。研究结果表明,在适宜的沉淀剂浓度、复合乳化剂、Fe3O4经油酸改性等条件下,可以制得平均粒径为150 nm、单分散性好且磁性明显的壳聚糖磁性微球。The Fe3O4 nanoparticles were prepared by co-precipitation method and modified by oleic acid.Chitosan magnetic microspheres were then prepared by precipitation polymerization method.The results demonstrated that the concentration of the precipitant,the type of emulsion and the modification of the iron oxide were the most effective parameters for the preparation of the microspheres.The SEM,IR and DLS results indicated that the diameter of the microspheres was about 150nm and the microspheres were uniformly distributed.The magnetic test showed the microspheres were magnetic sensitive.国家自然科学基金(50573063);; 教育部新世纪优秀人才计划项目;; 高等学校博士点专项基金(2005038401);; 厦门大学人才基金(0000X071C1

Production and consumption of dimethyl sulfur compounds by three typical algae

采用抑制剂加入法估算了中肋骨条藻、棕囊藻和东海原甲藻在不同生长期内二甲基硫化物的产生与消耗速率.结果表明:颗粒态二甲基巯基丙酸(DMSPp)和颗粒态二甲亚砜(DMSOp)在3种藻类的不同生长期内均为净消耗,溶解态二甲基巯基丙酸(DMSPd)和溶解态二甲亚砜(DMSOd)的含量受藻类产生与细菌病毒消耗控制,在藻类不同生长期内存在不同的产生与消耗速率,而二甲基硫(DMS)在3种藻不同生长期内均为净产生.同一种藻在不同生长期内以及不同藻在相同生长期内二甲基硫化物的产生与消耗速率均存在较大差异,表明藻类的生理状态和种间差异均对二甲基硫化物的产生与消耗速率产生影响.By the method of inhibitor amendment, this paper estimated the production and consumption of dimethyl sulfur compounds by Skeletonema costatum, Phaeocystis sp. and Prorocentrum donghaiense in their different growth phases. The results indicated that throughout the whole growth period of the three algae, particle dimethylsulfoniopropionate (DMSPp) and dimethylsulfoxide (DMSOp) were net consumed. The concentrations of dissolved dimethylsulfoniopropionate (DMSPd) and dimethylsulfoxide (DMSOd) were mainly affected by the processes of algal production and bacterial and virus consumption, and thus, could reflect their production or consumption rates in the growth period of algae. Dimethylsulfide (DMS) was net produced in the whole growth period of the algae. There was a greater difference in the production and consumption of dimethyl sulfur compounds for the same alga in its different growth phases and for different algae in the same growth phase, indicating that both the physiology and the inter-species difference of algae had effects on the production and consumption of dimethyl sulfur compounds.国家自然科学基金资助项目(200077022

一种新型玻璃萃取装置

本文介绍了一种新的、简便易行的玻璃萃取装置。与常规分液漏斗相比，该装置操作省时省力、无漏、重现性好。用于分光光度法测定挥发性酚时，重现性较现有方法大为改善

铁蛋白反应器储存有毒金属离子的初步研究

探讨铁蛋白反应器在模拟流动海水体系中储存有毒金属离子的能力及规律.在体外,脱铁核铁蛋白能重新构建新的铁核且核中多数铁组分经含有5% 氯化钠的海水处理后仍可稳定于蛋白壳中,只有少量对H+ 或OH- 较敏感的铁组分随反应体系的pH值增加或减少而被直接释放于介质中.此外,铁蛋白还能储存Co2+ 、Ni2+ 、Mn2+ 和Zn2+ 等有毒金属离子,并释放相对应的铁量,其储存离子量和释放Fe3+ 量的摩尔比为1:1,该储存量及能力与环境介质的pH值有关,与Na+ 、K+ 、Ca2+ 等非过渡金属离子无关.经改造后的铁蛋白反应器预计可用于监测流动水域的有毒金属离子

Preparation and Characterization of p(NIPAM-co-AA)/TiO_2 Composite Microspheres

用溶胶凝胶法制备了二氧化钛(TIO2)纳米粒子,并用硅烷偶联剂γ-甲基丙烯酰氧基丙基三甲氧基硅烷(γ-MPS)对其表面进行改性,继而采用光引发自由基聚合制备了聚(n-异丙基丙烯酰胺-CO-丙烯酸)/TIO2复合微球。考察了共聚单体丙烯酸(AA)、表面活性剂司盘80(SPAn80)在反应体系中的加入量对制备微球的影响,并比较了不同干燥方法对微球形态的影响。结果表明:合适的单体加入量、表面活性剂用量以及干燥方法可制备出分散性和球形度较好的复合微球。TiO2 nanoparticles were prepared by sol-gel method and modified with γ-MPS.Poly(NIPAM-co-AA)/TiO2 was prepared via UV-initiated radical polymerization.The effects of AA and Span80 contents,and different drying methods were investigated.The results demonstrated that the content of the co-monomer,the concentration of the surfactant and the drying method played significant roles in the preparation of the microspheres.国家自然科学基金项目(No.20976145

Experimental studies on treating humid acid solution by photocatalytic oxidation

以腐殖酸类物质作为废水中有机物的代表,研究了水体中腐殖酸在纳米TIO2颗粒上的吸附行为及以此为催化剂对水中腐殖酸进行的光催化氧化行为。考察了不同催化剂质量浓度、初始PH值、腐殖酸初始质量浓度等因素对腐殖酸去除率的影响。结果表明:腐殖酸在TIO2颗粒上的平衡吸附量对溶液的初始PH值依赖性很小;初始PH值降低、腐殖酸初始质量浓度减小,均能显著提高腐殖酸的去除率。同时将光催化氧化与单纯紫外线照射的去除率作对比,光催化氧化法明显优于单纯紫外线照射。Humid acid(HA) is the representation of organic matter in wastewater.The behaviors of adsorption and photocatalytic oxidation of HA in water on TiO2 particles were studied.The effects of different factors on removal of HA from water were investigated,such as mass concentration of catalyst,initial value of pH,initial mass concentration of HA,etc.The results show that the adsorption amount of HA in balance on TiO2 particles is independent of initial pH value of the solution.The removal efficiency of HA is improved greatly with the decreasing of initial pH value and initial mass concentration of HA.Comparing the removal efficiency of photocatalytic oxidation with that of ultraviolet radiation alone,the photocatalytic oxidation is better than ultraviolet radiation alone.厦门大学人才引进基金项目(0000X071C1

Impact of substrate salinity on caloric value,energy accumulation and its distribution in various organs of Bruguiera gymnorrhiza seedlings.

研究了人工培养条件下基质盐度对红树植物木榄 (Bruguieragymnorrhiza)幼苗的热值、干物质及能量积累的影响 .结果表明 ,木榄幼苗干物质和能量的积累存在低盐促进和高盐抑制的现象 ,其最大值出现于盐度10mg·g-1处 ;随基质盐度的提高 ,木榄幼苗的干物质和能量积累倾向于叶片及细根等光合作用场所和养分吸收器官 ,木榄幼苗各器官干重热值及去灰分热值有不同的变化规律 ,其中叶片热值的变化规律能较好地反映木榄幼苗抗盐性的变化情况 .本文从能量学角度探讨了红树植物的抗盐生理生态The study with artificial culture showed that the accumulation of dry matter and energy in Bruguiera gymnorrhiza seedlings was promoted by low substrate salinity,but inhibited by high substrate salinity,which peaked at 10mg·g -1 substrate salinity.With the increase of substrate salinity, the dry matter and the energy tended to be allocated to the micro roots and leaves, which are nutrient absorbing organ and photosynthetic organ, respectively.With the increase of substrate salinity, the changes in caloric values were differed in various organs of B. gymnorrhiza seedlings,and those in leaves could reflect the changes in the salt resistance of the seedlings.国家自然科学基金资助项目!(39670 1 35

猪血管紧张肽的质谱特性

选用基质辅助激光解吸离子化飞行时间质谱技术研究不同激光强度对猪血管紧张肽 (AngiotensinⅠ ,AnⅠ )解吸离子化的百分率和一级结构的稳定性。结果表明 :激光强度与AnⅠ解吸离子化百分率有关 ,AnⅠ多聚体解吸离子化所需的激光强度高于单体。AnⅠ与海兔酸性多肽 (Aplysiaacidicpeptide,AP)和海兔胰岛素Cβ(AplysiainsulinCβ,AICβ)混合后 ,采用延迟引出技术和激光强度 2 3 0 0IU分析混合样品 ,该激光强度不影响对AnⅠ和它的多聚体解吸离子化 ,但对AICβ 影响较大 ,相当于丢失了一分子OH-。相同的激光强度对不同结构的多肽多聚体产生不同的解吸离子化百分率 ,优化激光强度是准确分析多肽结构信息关键因素之一