镰形棘豆黄酮类化合物对紫外线辐射引起的角质形成细胞损伤的保护作用

目的探讨镰形棘豆黄酮类化合物对中波紫外线照射所致人角质形成细胞损伤的保护作用。方法体外培养人角质形成细胞系,以60、90、120 mJ/cm2中波紫外线照射,加入镰形棘豆黄酮类化合物进行干预处理,倒置相差显微镜观察细胞受损程度,以MTT法检测细胞增殖活性,ELISA检测上清液TNF-α、IL-10分泌量,实时荧光定量PCR检测TNF-αmRNA、IL-10 mRNA表达。结果中波紫外线照射后,细胞形态受损,随着照射剂量增加,细胞增殖活性下降,损伤程度加重,TNF-α、IL-10分泌量增加,TNF-αmRNA、IL-10 mRNA表达水平增加。加入镰形棘豆黄酮类化合物干预后,细胞活性有所恢复,TNF-α、IL-10分泌量降低,TNF-αmRNA、IL-10 mRNA表达水平下调,具有显著性差异。结论镰形棘豆黄酮类化合物具有一定的光保护作用,抑制炎症细胞因子分泌可能是其减轻紫外线辐射损伤的机制之一

HPLC法测定藏药镰形棘豆中鼠李柠檬素-3-O-β-新橙皮糖苷的含量

目的:建立镰形棘豆中鼠李柠檬素-3-O-β-新橙皮糖苷的含量测定方法,同时比较药材的不同部位经不同溶剂提取后所得含量。方法:采用HPLC法,色谱柱:Symmetry C18柱(4.6mm×250mm,5μm);流动相:1%乙酸水溶液-乙腈(79:21);流速:1.0mL/min;检测波长:350nm。结果:鼠李柠檬素-3-O-β-新橙皮糖苷在20-100μg/mL范围内线性关系良好(r=0.9995),平均回收率为99.49%,RSD=1.30%。溶剂对该化合物的提取率由高至低依次为甲醇,95%乙醇、水;药材叶中含量比根高。结论:本法简便、准确、重现性好,可用于该药材或相关制剂的质量控制;药材不同部位经不同溶剂提取所得含量差别较大

Vapor-liquid-equilibrium and volume expansion calculations of CO_2/acetone,CO_2/ethanol and CO_2/ethanol/water at high pressure by using t-mPR equation of state

作者简介:蒋茂星(1983－),女,助教,主要研究方向:超临界流体技术 通信联系人:李军,教授,主要研究方向:超临界流体技术,[email protected][中文文摘]采用t-mPR状态方程(EoS)对CO2/丙酮、CO2/乙醇二元体系和CO2/乙醇/水三元体系的高压汽液平衡(VLE)、液相体积膨胀率进行计算,并与文献报道的实验数据,以及采用PR EoS得到的结果进行比较。结果表明,与PR EoS相比较,t-mPR EoS可以对上述二元体系的VLE取得较好的关联效果,对三元体系取得较好的预测效果。基于VLE的计算,进一步对上述体系的液相体积膨胀率进行预测。与PREoS相比较,t-mPREoS对二元体系的预测结果的精度有所提高,对三元体系在7.8MPa左右有最大体积膨胀率,模型预测较好。[英文文摘]The vapor-liquid equilibirum (VLE) and volume expansion of the liquid phases for the CO2/acetone and CO2/ethanol binary systems and the CO2/ethanol/water ternary system have been investigated using the t-mPR equation of state (t-mPR EoS). Calcualted results have been compared with published experimental data and those obtained from the PR equation of state (PR EoS). The correlations for the published VLE data of the above binary systems and the prediction for the ternary system showed that both the t-mPR EoS and the PR EoS can provide fairly good VLE calculations, but t-mPR EoS gives slightly better results. The volume expansion prediction for the above systems and the comparison with the published data showed that the t-mPR EoS gives better results to PR-EoS for the binary systems and provides more reasonable results around 7.8 MPa (there exists a maximum volume expansion) for the ternay system.高等学校博士学科点专项科研基金资助项目(20100121110009

正交试验优选镰形棘豆总黄酮活性炭脱色工艺

目的优选镰形棘豆总黄酮活性炭脱色工艺。方法以温度、时间和活性炭用量为考察因素,脱色液颜色、260～420nm紫外吸收情况和总黄酮含量为考察指标,采用正交试验法探究最佳脱色条件。结果脱色最佳工艺为在40℃下,加入1%活性炭,搅拌5min。结论该脱色工艺可行,既能达到脱色效果,又能保留有效成分

Production of Polyethylene Glycol Microparticles Using a SAS-Atomization Drying Process

超临界流体抗溶剂-雾化(SAS-A)技术研究用聚乙二醇(PEG6000)/乙醇/水体系制备聚乙二醇(PEG)微粒。探讨预膨胀压力、溶液浓度和溶液流量等工艺参数对微粒形貌及粒径的影响;重点探讨SAS-A技术中使用不同溶剂对颗粒形态和粒径分布的影响。结果表明,以丙酮和乙醇为溶剂的SAS-A技术可以制得形态基本上为球形的PEG微粒,粒径分布分别可以控制在1~5μm和2~15μm之间;增大预膨胀压力容易得到分散的球形微粒,并能减小微粒粒径,微粒粒径分布也随之变窄。对应PEG/丙酮体系,溶液浓度升高,所得到的微粒粒径增大;对应PEG/乙醇体系,溶液浓度对粒径大小影响不大,但溶液浓度增大会使粒径分布变宽。采用乙醇水溶液为溶剂时,初始乙醇浓度越低,移出水的效果越差,易形成结块的不规则微粒。Supercritical anti-solvent (SAS)-atomization process was employed to produce Polyethylene Glycol particles from PEG6000/ethanol/water solutions. The effect of different process parameters on the morphology and the size of the produced PEG particles, including the operating pressure, the protein concentration and the flow-rate of the protein/ethanol/water feed had been studied, especially the influence of various solvents were evaluated. The PEG particles produced form acetone and ethanol were generally spherical and with sizes of 1-5μm and 2-15μm respectively. Increasing pre-expansion pressure produced spherical and discrete particles with small size and narrow size distribution. Increasing the PEG concentration in acetone could evidently increase the PEG particle sizes and widen the particle size distribution while in ethanol the PEG particle sizes were changeless. Decreasing the concentration of ethanol in ethanol/water solution generated the particles irregular and agglomerated as water removal was limited