代谢中间物对斜生栅藻叶绿素荧光特性以及油脂积累的研究

为了研究代谢中间物(柠檬酸与苹果酸)的添加对斜生栅藻(Scenedesmus obliquus XJ002)光合效率以及胞内油脂积累的影响,研究了代谢中间物的添加对XJ002藻株生物量、光合色素、叶绿素荧光特性以及胞内总脂含量的影响。结果表明:添加高浓度代谢中间物导致藻细胞生物量与光合色素含量显著下降,损坏藻细胞光系统II反应中心供体侧的放氧复合体(OEC)结构,抑制光系统II反应中心受体侧电子传递活性。当柠檬酸与苹果酸处理浓度为0.15 g/L时,XJ002藻株胞内总脂含量是对照组的1.85倍和1.34倍。高浓度代谢中间物的添加,显著提高了XJ002藻株胞内总脂含量,与苹果酸相比,柠檬酸的添加效果更佳。国家自然科学基金(41666007);;内蒙古自治区科技计划项目(201601061);;内蒙古科技大学大学生创新基

Determination of Lead Content in Standard Materials of Lake Sediment and Tea by ID-ICP-MS

采用2种微波消解体系(HNO3-H2O2和HNO3-H2O2-HF)及3种定量模式(同位素稀释法、标准工作曲线法和标准加入法),对沉积物和茶叶标准物质中的铅进行测定,结果表明:同位素稀释质谱法的各项检测指标显著优于其它两种定量模式(茶叶中铅含量测定值为4.30μg/g),标准加入法次之(3.75μg/g),标准工作曲线法较差(3.61μg/g)。二种微波消解体系中,加HF酸优于不加HF酸,不加HF,茶叶中铅的回收率仅有89%,加入HF则上升为98%。Three different quantitative modes,namely external calibration,standard addition and isotope diluˉtion were compared for the determination of lead contents in lake sediment and tea standard materials with two digestion systems.The results showed that the isotope dilution gave the highest accuracy and precision in comˉparison with the other two modes.But the results were affected by the digestion system.For example,the reˉcoveries of lead content for tea were98%and89%for HNO 3 -H 2 O 2 -HF and HNO 3 -H 2 O 2 digesting sysˉtem,respectively.福建省重大科技项目子课题(2003Y005-04);; 厦门大学现代分析科学教育部重点实验室开放课题(B40402

Study on the Distributions of Lead and Cadmium in Rats by ICP-MS with Stable Isotope Tracer

采用稳定同位素示踪技术研究重金属元素铅、镉在大鼠体内的分布,通过对大鼠进行浓缩同位素206Pb、112Cd灌喂,解剖取其肝、肾、睾丸和心脏等脏器,通过相应的化学处理,用电感耦合等离子体质谱(ICP-MS)测量铅、镉元素在各个脏器中的含量及其同位素组成变化。根据同位素比值的变化研究Pb、Cd在各脏器中的分布、蓄积规律和各脏器对引入的浓缩同位素利用率。结果发现:重金属Pb和Cd蓄积的最主要场所是肝和肾,其次是睾丸和心脏,而在脑中的积累最少。在肝和肾中Cd的同位素利用率为0.0488%、0.0154%,Pb的同位素利用率为0.0101%、0.0078%,远高于其它脏器。与传统的方法相比较,该法具有更接近慢性中毒、危害性小,能将重金属来源分为天然来源和人为引入等特点。The distributions of lead and cadmium in rats were studied using stable isotope tracer technique.The rats were fed with enriched stable isotope 206Pb and 112Cd,then the liver,kidney,testicle and heart were eviscerated.After chemical treatment,the contents of lead and cadmium and their isotope ratios in each viscera were determined by ICP-MS.Experimental results showed that lead and cadmium accumulated mostly in liver and kidney,next in testicle and heart,and least in brain.The utilization rates of cadmium and lead isotopes in liver were 0.048 8% and 0.010 1%,respectively,and in kidney 0.015 4% and 0.007 8%,respectively.Compared with the traditional methods,the present method can differentiate the origins of lead and cadmium.福建省重大科技项目资助课题(2003Y005-04,2003Y003

Use of stable tracer for studying accumulation and speciation of heavy metals in rat tissues

随着工农业生产的发展，有毒重金属对人体的潜在危害越来越引起了人们的普遍关注，研究重金属在生物体内的迁移转化规律和存在形态是阐明其毒理学和其他生物效应的重要依据。采用高浓度胁迫和使用单剂量胁迫试剂对实验动物进行染毒（注射或灌喂）是最常用的研究毒物在动物体内的分布和代谢手段。本工作采用低剂量富集稳定同位素（Pb和Cd）结合电感耦合等离子体质谱（ICP-MS）对大鼠进行示踪研究，建立HPLC与ICP-MS联用对大鼠体内的金属硫蛋白进行分离和表征。论文共由四章组成：1.文献综述；2.建立同位素稀释电感耦合等离子体质谱测定环境和生物样品中镉的方法；3.稳定同位素示踪在大鼠各组织器官中重金属的分布研究；4...In the present day, heavy metals has always been an important role because of its’ harmfulness to people’s health. The sduty of dynamic distribution of heavy metals in animals is necessary in toxicology. In this work, a method using enriched stable isotope tracer and induced coupled plasma mass spectrometry(ICP-MS) was developed to investigate the distribution of Pb and Cd in a variety of rat org...学位：理学硕士院系专业：化学化工学院化学系_分析化学学号：20032501

Research into the value of Jiangxi＇s merging into the Changjiang economic belt under the background of ＂one belt and one road＂

江西的发展对长江经济带乃至“一带一路”战略,都具有积极意义。江西作为长江中游重要省份,它的发展能更好地推动长江上中下游的联动与合作,有助于实现＂平衡发展、共同繁荣＂战略目标;而长江经济带的整合有利于更好地发挥长江黄金水道的连接作用,对“一带一路”战略形成助力。江西省要结合自身特点,将发展融入国家战略,走出独一无二的发展道路,建设长江经济带的新增长极。The development of Jiangxi if of great significance for the the Changjiang economic belt and ＂one belt and one road＂ strategy. As an important province in the middle reach of Changjiang River, its development can promote the connection and cooperation and helps realize the strategic goal of ＂balance development and common prosperity＂. The integration of the Changjiang economic belt is ad- vantageous for playing linking role of Changjiang as gold water course and helps the ＂one belt and one road＂ strategy. Jiangxi province should be based on its characteristics, integrate its development into the national strategy and embark on unique development road and build the new increase pole of the Changjiang economie belt

一株高效生物絮凝剂产生菌B17的培养优化

从活性污泥中筛选得到的一株高活性的生物絮凝剂产生菌B17,采用单因素实验优化培养时间、碳源、氮源、碳氮比、初始pH值、接种量等培养条件,选择乳糖作为碳源,乙酸铵为氮源,碳氮比为20:1,发酵初始pH值为6.0-7.0,接种量为3%,在此最优组合的发酵条件下以温度30℃振速160rpm培养24h,絮凝活性可提高25%-38.3%

酵母型生物絮凝剂产生菌的筛选及培养基优化

目的:获得高效的酵母菌型絮凝剂。方法:以代表性的高岭土悬浊液、养殖场废水、土壤上清液、藻液、硫酸铜溶液、发酵废水作为测试废水,筛选具有絮凝活性的菌种。结果:筛选出3株具有絮凝活性的菌种,其中酵母1-4絮凝效果较好且适用范围较广;对酵母1-4菌培养基的碳源、氮源、碳氮比进行优化,确定最适碳源为甘露醇,最适氮源为氯化铵,碳氮比5∶1为最佳。结论:用醇降法提取优化培养所得的絮凝剂,产率为5.0g/L,经紫外吸收光谱和茚三酮反应初步判断絮凝剂的主要成分为蛋白质

黄土丘陵区土地利用的竞争模式

对土地利用竞争模式进行了论述,根据上黄试区22年统计数据,分析了人口增长及其土地利用变化。结果表明:政策因素是环境响应的主体,并在控制土地利用的方向上起积极作用;技术因素可以缓解土地利用的竞争压力,深刻影响土地变化,改变土地利用的竞争格局;传统特色农业及以技术进步为基础的特色农业是现在及未来黄土丘陵区土地利用的发展方向

ICP-MS Simultaneous Determination of Inorganic Elements in Loquat Leaves

枇杷叶试样于聚四氟烯消解罐中加入硝酸及过氧化氢置于微波炉中加热消解,对所得试样溶液中的14种无机元素用电感耦合等离子体质谱法同时测定。在测定钙、锰、镁、磷及铁的试验中,测得其线性范围在0~200μg.L-1之间,用标准加入法对所测定的14种无机元素作了回收试验,所得回收率在90%~110%之间。按规定要求测定14种无机元素的检出限,测得结果在0.002~0.4μg.L-1之间。Sample of loquat leaves was digested with HNO3 and H2O2 in PTFE vessel heated in microwave oven.Fourteen inorganic elements in the sample solution were determined by ICP-MS.As shown by the data of determination of Ca,Mn,Mg,P and Fe,ranges of linearity were obtained in the range of 0-200 μg·L-1.Tests for recovery were carried out by standard addition method and values of recovery for the 14 elements determined were in the range of 90%-110%.Detection limits for these elements were in the range from 0.002 to 0.4 μg·L-1

中国林业产业结构与经济增长关系的模型解析

本文运用1996~2012年中国林业产业产值和内部第一、二、三产业的面板数据,采用固定效应模型对林业产值增长进行空间分析。估计结果表明,第三产业和林业生产能力对林业产值有显著的正向影响,第二产业、耕地面积和人均生产总值对林业产值提升存在负面影响。最后,本文提出促进林业产业发展的对策与建议