Efficient generation of >2 W of green light by single pass frequency doubling in PPMgLN

We report 32% efficient frequency doubling of single frequency 1029 nm light to green light at 514.5 nm using a single pass configuration. A congruent composition, periodically poled magnesium doped lithium niobate (PPMgLN) crystal of 50 mm length was used to generate a second harmonic power of 2.3 W. To our knowledge, this is the highest reported frequency doubling efficiency of any wavelength light in a PPMgLN crystal and also the highest reported SHG output power in the green for PPMgLN.Comment: 5 pages, 3 figures. Submitted to Optics Express, awaiting respons

Assembly of phage Mu transpososomes: Cooperative transitions assisted by protein and DNA scaffolds

AbstractTransposition of phage Mu takes place within higher order protein-DNA complexes called transpososomes. These complexes contain the two Mu genome ends synapsed by a tetramer of Mu transposase (MuA). Transpososome assembly is tightly controlled by multiple protein and DNA sequence cofactors. We find that assembly can occur through two distinct pathways. One previously described pathway depends on an enhancer-like sequence element, the internal activation sequence (IAS). The second pathway depends on a MuB protein-target DNA complex. For both pathways, all four MuA monomers in the tetramer need to interact with an assembly-assisting element, either the IAS or MuB. However, once assembled, not all MuA monomers within the transpososome need to interact with MuB to capture MuB-bound target DNA. The multiple layers of control likely are used in vivo to ensure efficient rounds of DNA replication when needed, while minimizing unwanted transposition products

Regulation of DNA supercoiling in Escherichia coli: Genetic basis of a compensatory mutation in DNA gyrase

AbstractBacterial DNA supercoiling is controlled by balancing the supercoiling activity of DNA gyrase and the relaxing activity of DNA topoisomerase I. We have characterized the gyrB gene from a topA deletion mutant of Escherichia coli (DM800) that has a compensatory mutation in gyrB, lowering the activity of gyrase 10-fold, and thereby redressing the intracellular level of supercoiling. The mutant gene differs from the wild type in carrying three rather than two direct tandem repeats of a 6 bp sequence encoding Ala-Arg. We suggest this novel mutation affects domain spacing and was generated by an unequal crossing over event, possibly involving gyrase