Where the rainbow ends: The hidden humanitarian crisis for members of the LGBTQIA+ community in international business

There are several people I would like to thank for their assistance in my Honors Capstone research and the writing of this Honors Thesis. First and foremost, I would like to thank my project advisor, Professor Fernando Pargas, for his assistance in planning my research and his continued support and input over the past few months. I would also like to thank my readers, Dr. Laura Leduc and Professor Robert Eliason for offering up their time to read my thesis and provide insightful feedback. Additionally, I would like to thank Elizabeth Price, the research librarian for the College of Business at James Madison University for her help in finding the most up-to-date sources for my law review and assisting me in crafting the bibliography. Lastly, I would like to thank my family for their continued support throughout this long, demanding process; it would not have been possible to create a project of such magnitude without them

Keywords of the manosphere

This paper examines language used in five of the largest manosphere communities on Reddit (r/TheRedPill, r/braincels, r/MensRights, r/seduction, and r/MGTOW) to identify idiosyncratic language use within these communities. To do so, a novel methodology which combines key-key-word analysis with notions from set theory was used to identify and compare keywords between corpora and to find keywords that are used uniquely within – and thus are distinctive to – these five separate communities. The paper achieves the following: it (i) presents a novel method for identifying what we term ‘complement keywords’ (keywords that are not shared between multiple different corpora when compared against the same reference corpus), and (ii) explores idiosyncratic language use in five separate manosphere communities. The analysis first examines interdiscursive relationships between communities emerging from the complement keywords identified before discussing community-specific preoccupations emergent in the idiosyncratic language use found in these five communities

Keywords of the manosphere

This paper examines language used in five of the largest manosphere communities on Reddit (r/TheRedPill, r/braincels, r/MensRights, r/seduction, and r/MGTOW) to identify idiosyncratic language use within these communities. To do so, a novel methodology which combines key-key-word analysis with notions from set theory was used to identify and compare keywords between corpora, and finds keywords that are used uniquely within – and thus are distinctive to – these five separate communities. The paper achieves the following: i) presents a novel method for identifying what we term complement keywords (keywords that are not shared between multiple different corpora when compared against the same reference corpus), and ii) explores idiosyncratic language use in five separate manosphere communities. The analysis first examines interdiscursive relationships between communities emerging from the complement keywords identified before discussing community-specific preoccupations emergent in the idiosyncratic language use found in these five communities

The representation of gendered social actors across five manosphere communities on Reddit

This paper investigates the representation of gendered social actors in a specialised corpus of 10.9 million words collected from five Reddit communities associated with the so-called manosphere: incels (involuntary celibates), Men Going Their Own Way (male separatists), pick-up artists, men’s rights activists, and a group dedicated to wider discussions of ‘red pill’ philosophy. 34 gendered social actor terms were identified as key-key-words across the manosphere corpora. Both male and female social actors are referenced using relational terms, while the latter are also referenced using derogatory terms and the former are referenced using terms for kinship and in-group identification. We then analyse the consistent collocates (Baker et al., 2008) of the four most frequent gendered social actor terms (women, girls, men and guys), to establish the topics, descriptions, and actions associated with the social actors across the five groups. Gendered social actors were constructed in essentialist dichotomies, with women and girls, although objectified and passivated in dating/sexual contexts, being represented as violent towards male social actors and as holding a privileged position over men in wider society. The anti-feminist ideology reflected in manosphere discourse can be seen as a more extreme version of mainstream discourse, into which it may be re-imported

Three-dimensional electron microscopy reveals the evolution of glomerular barrier injury

Open access articleGlomeruli are highly sophisticated filters and glomerular disease is the leading cause of kidney failure. Morphological change in glomerular podocytes and the underlying basement membrane are frequently observed in disease, irrespective of the underlying molecular etiology. Standard electron microscopy techniques have enabled the identification and classification of glomerular diseases based on two-dimensional information, however complex three-dimensional ultrastructural relationships between cells and their extracellular matrix cannot be easily resolved with this approach. We employed serial block face-scanning electron microscopy to investigate Alport syndrome, the commonest monogenic glomerular disease, and compared findings to other genetic mouse models of glomerular disease (Myo1e−/−, Ptpro−/−). These analyses revealed the evolution of basement membrane and cellular defects through the progression of glomerular injury. Specifically we identified sub-podocyte expansions of the basement membrane with both cellular and matrix gene defects and found a corresponding reduction in podocyte foot process number. Furthermore, we discovered novel podocyte protrusions invading into the glomerular basement membrane in disease and these occurred frequently in expanded regions of basement membrane. These findings provide new insights into mechanisms of glomerular barrier dysfunction and suggest that common cell-matrix-adhesion pathways are involved in the progression of disease regardless of the primary insult

Membrane-cytoskeletal crosstalk mediated by myosin-I regulates adhesion turnover during phagocytosis

Phagocytosis of invading pathogens or cellular debris requires a dramatic change in cell shape driven by actin polymerization. For antibody-covered targets, phagocytosis is thought to proceed through the sequential engagement of Fc-receptors on the phagocyte with antibodies on the target surface, leading to the extension and closure of the phagocytic cup around the target. We find that two actin-dependent molecular motors, class 1 myosins myosin le and myosin if, are specifically localized to Fc-receptor adhesions and required for efficient phagocytosis of antibody-opsonized targets. Using primary macrophages lacking both myosin le and myosin if, we find that without the actin-membrane linkage mediated by these myosins, the organization of individual adhesions is compromised, leading to excessive actin polymerization, slower adhesion turnover, and deficient phagocytic internalization. This work identifies a role for class 1 myosins in coordinated adhesion turnover during phagocytosis and supports a mechanism involving membrane-cytoskeletal crosstalk for phagocytic cup closure

Coupled myosin VI motors facilitate unidirectional movement on an F-actin network

A combination of experimentation and modeling reveal that multiple myosin VI molecules coordinately transport cargo over the actin filament network

Myosin 1E localizes to actin polymerization sites in lamellipodia, affecting actin dynamics and adhesion formation

Because the actin network in active lamellipodia is continuously assembling at the edge, moving inward and disassembling, there is a question as to how actin-binding proteins and other components are transported to the leading edge and how nascent adhesions are stabilized. Active transport could play a significant role in these functions but the components involved are unknown. We show here that Myosin 1E (a long tailed Myosin 1 isoform) rapidly moves to the tips of active lamellipodia and to actin-rich early adhesions, unlike Myosin 1G, 1B or 1C (short tailed isoforms). Myosin 1E co-localizes with CARMIL, FHOD1, Arp3 and β3-integrin in those early adhesions. But these structures precede stable paxillin-rich adhesions. Myosin 1E movement depends upon actin-binding domains and the presence of an SH3 oligomerization domain. Overexpression of a Myosin 1E deletion mutant without the extreme C-terminal interacting (SH3) domain (Myosin 1EΔSH3) increases edge fluctuations and decreases stable adhesion lifetimes. In contrast, overexpression of Myosin 1E full tail domain (TH1+TH2+TH3/SH3) decreases edge fluctuation. In Myosin 1E knockdown cells, and more prominently in cells treated with Myosin 1 inhibitor, cell-matrix adhesions are also short-lived and fail to mature. We suggest that, by moving to actin polymerization sites and early adhesion sites in active lamellipodia, Myosin 1E might play important roles in transporting not only important polymerizing proteins but also proteins involved in adhesion stabilization.link_to_OA_fulltex

Gene Expression Profiling on Global cDNA Arrays Gives Hints Concerning Potential Signal Transduction Pathways Involved in Cardiac Fibrosis of Renal Failure

Cardiac remodelling with interstitial fibrosis in renal failure, which so far is only poorly understood on the molecular level, was investigated in the rat model by a global gene expression profiling analysis. Sprague–Dawley rats were subjected to subtotal nephrectomy (SNX) or sham operation (sham) and followed for 2 and 12 weeks, respectively. Heart-specific gene expression profiling, with RZPD Rat Unigene-1 cDNA arrays containing about 27 000 gene and EST sequences revealed substantial changes in gene expression in SNX compared to sham animals. Motor protein genes, growth and differentiation markers, and extracellular matrix genes were upregulated in SNX rats. Obviously, not only genes involved in cardiomyocyte hypertrophy, but also genes involved in the expansion of non-vascular interstitial tissue are activated very early in animals with renal failure. Together with earlier findings in the SNX model, the present data suggest the hypothesis that the local renin–angiotensin system (RAS) may be activated by at least two pathways: (a) via second messengers and Gproteins (short-term signalling); and (b) via motor proteins, actins and integrins (longterm signalling). The study documents that complex hybridization analysis yields reproducible and promising results of patterns of gene activation pointing to signalling pathways involved in cardiac remodelling in renal failure. The complete array data are available via http://www.rzpd.de/cgi-bin/services/exp/viewExpressionData.pl.cg