Three-dimensional imaging of direct-written photonic structures

Third harmonic generation microscopy has been used to analyze the morphology of photonic structures created using the femtosecond laser direct-write technique. Three dimensional waveguide arrays and waveguide-Bragg gratings written in fused-silica and doped phosphate glass were investigated. A sensorless adaptive optical system was used to correct the optical aberrations occurring in the sample and microscope system, which had a lateral resolution of less than 500 nm. This non-destructive testing method creates volume reconstructions of photonic devices and reveals details invisible to other linear microscopy and index profilometry techniques.Comment: 8 pages, 3 color figures, 2 hyper-linked animation

Four-dimensional light shaping: manipulating ultrafast spatio-temporal foci in space and time

Spectral dispersion of ultrashort pulses allows simultaneous focusing of light in both space and time creating so-called spatio-temporal foci. Such space-time coupling may be combined with existing holographic techniques to give a further dimension of control when generating focal light fields. It is shown that a phase-only hologram placed in the pupil plane of an objective and illuminated by a spatially chirped ultrashort pulse can be used to generate three dimensional arrays of spatio-temporally focused spots. Exploiting the pulse front tilt generated at focus when applying simultaneous spatial and temporal focusing (SSTF), it is possible to overlap neighbouring foci in time to create a smooth intensity distribution. The resulting light field displays a high level of axial confinement, with experimental demonstrations given through two-photon microscopy and non-linear laser fabrication of glass

Katja Kansteiner/ Christoph Stamann/ Claus G. Buhren/ Peter Theurl (Hrsg.): Professionelle Lerngemeinschaften als Entwicklungsinstrument im Bildungswesen. Weinheim Basel: Beltz Juventa 2020 (300 S.) [Rezension]

Rezension von: Katja Kansteiner/ Christoph Stamann/ Claus G. Buhren/ Peter Theurl (Hrsg.): Professionelle Lerngemeinschaften als Entwicklungsinstrument im Bildungswesen. Weinheim Basel: Beltz Juventa 2020 (300 S.; ISBN 978-3-7799-6047-8; 39,95 EUR)

Holographic aberration correction: optimising the stiffness of an optical trap deep in the sample

We investigate the effects of 1st order spherical aberration and defocus upon the stiffness of an optical trap tens of μm into the sample. We control both these aberrations with a spatial light modulator. The key to maintain optimum trap stiffness over a range of depths is a specific non-trivial combination of defocus and axial objective position. This optimisation increases the trap stiffness by up to a factor of 3 and allows trapping of 1μm polystyrene beads up to 50μm deep in the sample.<p></p&gt

Characterisation of the dynamic behaviour of lipid droplets in the early mouse embryo using adaptive harmonic generation microscopy

<p>Abstract</p> <p>Background</p> <p>Lipid droplets (LD) are organelles with an important role in normal metabolism and disease. The lipid content of embryos has a major impact on viability and development. LD in Drosophila embryos and cultured cell lines have been shown to move and fuse in a microtubule dependent manner. Due to limitations in current imaging technology, little is known about the behaviour of LD in the mammalian embryo. Harmonic generation microscopy (HGM) allows one to image LD without the use of exogenous labels. Adaptive optics can be used to correct aberrations that would otherwise degrade the quality and information content of images.</p> <p>Results</p> <p>We have built a harmonic generation microscope with adaptive optics to characterise early mouse embryogenesis. At fertilization, LD are small and uniformly distributed, but in the implanting blastocyst, LD are larger and enriched in the invading giant cells of the trophectoderm. Time-lapse studies reveal that LD move continuously and collide but do not fuse, instead forming aggregates that subsequently behave as single units. Using specific inhibitors, we show that the velocity and dynamic behaviour of LD is dependent not only on microtubules as in other systems, but also on microfilaments. We explore the limits within which HGM can be used to study living embryos without compromising viability and make the counterintuitive finding that 16 J of energy delivered continuously over a period of minutes can be less deleterious than an order of magnitude lower energy delivered dis-continuously over a period of hours.</p> <p>Conclusions</p> <p>LD in pre-implantation mouse embryos show a previously unappreciated complexity of behaviour that is dependent not only on microtubules, but also microfilaments. Unlike LD in other systems, LD in the mouse embryo do not fuse but form aggregates. This study establishes HGM with adaptive optics as a powerful tool for the study of LD biology and provides insights into the photo-toxic effects of imaging embryos.</p

Professionalisierung von Führungspersonen an Grundschulen

Die Rolle von schulischen Führungspersonen hat sich im deutschsprachigen Raum spätestens mit dem sog. Neuen Steuerungsmodell nachhaltig verändert. Erweitert wurde das Aufgabenrepertoire von schulischen Führungspersonen, worauf diese jedoch nur in Ansätzen im Rahmen ihrer Ausbildung vorbereitet werden. Vor diesem Hintergrund diskutieren die Autorinnen die Professionalisierung von schulischen Führungspersonen und arbeiten auf Grundlage theoretischer sowie empirischer Befunde zu Grundschulen besondere Herausforderungen für Leitungshandeln in der Primarstufe heraus. (DIPF/Orig.

Optofluidic adaptive optics in multi-photon microscopy

Adaptive optics in combination with multi-photon techniques is a powerful approach to image deep into a specimen. Remarkably, virtually all adaptive optics schemes today rely on wavefront modulators which are reflective, diffractive, or both. This, however, can pose a severe limitation for applications. Here, we present a fast and robust sensorless adaptive optics scheme adapted for transmissive wavefront modulators. We study our scheme in numerical simulations and in experiments with a novel, optofluidic wavefront shaping device which is transmissive, refractive, polarisation-independent and broadband. We demonstrate scatter correction of two-photon-excited fluorescence images of microbeads as well as brain cells and benchmark our device against a liquid-crystal spatial light modulator. Our method and technology could open new routes for adaptive optics in scenarios where previously the restriction to reflective and diffractive devices may have staggered innovation and progress

Independent polarisation control of multiple optical traps

We present a system which uses a single spatial light modulator to control the spin angular momentum of multiple optical traps. These traps may be independently controlled both in terms of spatial location and in terms of their spin angular momentum content. The system relies on a spatial light modulator used in a "split-screen" configuration to generate beams of orthogonal polarisation states which are subsequently combined at a polarising beam splitter. Defining the phase difference between the beams with the spatial light modulator enables control of the polarisation state of the light. We demonstrate the functionality of the system by controlling the rotation and orientation of birefringent vaterite crystals within holographic optical tweezers

Holographic optical trapping

Holographic optical tweezers use computer-generated holograms to create arbitrary three-dimensional configurations of single-beam optical traps useful for capturing, moving and transforming mesoscopic objects. Through a combination of beam-splitting, mode forming, and adaptive wavefront correction, holographic traps can exert precisely specified and characterized forces and torques on objects ranging in size from a few nanometers to hundreds of micrometers. With nanometer-scale spatial resolution and real-time reconfigurability, holographic optical traps offer extraordinary access to the microscopic world and already have found applications in fundamental research and industrial applications.Comment: 8 pages, 7 figures, invited contribution to Applied Optics focus issue on Digital Holograph