RESEARCH ON THE THERMAL CONDUCTIVITY OF COMPOSITES MADE OF ECOLOGICAL FIBERS

The paper presents the results of the researchconducted to obtain new ecological composites thatcould be used for thermal insulation of buildings. Theobtained panels are made of ecological materials thatdo not affect the human health (wood chips andfibers, host of hemp, textile fibers, wool and reed).The testing was performed in eight points, for aninternal temperature of T=200C and an outdoortemperature situated in the range of -200C÷200C). Asthe tests conducted, the results showed that the bestinsulating capacity belonged to a composite whichhas wood fiber and wool in its structure, followed acomposite which has wood chips, hemp particles andwool in its structure

Design of antigen synthesis and identification of its artificial antigen for zearalenone

Background: study aimed to modify the ZEN molecule and conjugate the carrier protein to prepare a complete antigen. To lay the foundation for the preparation of ZEN monoclonal antibodies. Methods: The carbonyl group at the 7 position of ZEN molecule was modified by deuteration reaction. The immunogen and antigen were synthesized by EDC method and mixed acid anhydride method, and the complete antigen was identified by UV, IR and electrophoresis. Antisera were obtained after immunization of animals, and an antiserum was designed by ELISA. Results: The immunogens were identified by UV, IR and electrophoresis, ZEN-BSA was successfully synthesized. The ratio of ZEN-BAS to ZEN was calculated to be 1 : 13. When the antibody serum was detected, the titer of the antibody reached 1:(6.4×103). Conclusion: This study demonstrated that the OAE method is preferable in preparing the ZEN. These findings lay the material and technical foundation for the preparation of anti-ZEN monoclonal antibod

Determination of antimicrobial and fungicidal properties of experimental disinfectant "Sukhodez"

The results of the study of antimicrobial and fungicidal properties of the experimental preparation “Sukhodez” against microorganisms E. coli, Salmonella enteritidis, Staphylococcus aureus and fungi Candida albicans in the laboratory are presented. The aim of the research. To study the antimicrobial and fungicidal properties of powdered disinfectant and analyze the prospects for its further use in a set of anti-epizootic measures in particular in poultry farms. Materials and methods. The research was conducted during 2021 at the Department of Veterinary Examination, Microbiology, Zoohygiene and Safety and Quality of Animal Products of the Faculty of Veterinary Medicine of Sumy National Agrarian University. Evaluation of bactericidal properties of the experimental agent “Sukhodez” was determined on museum strains of E. coli ATCC 25922, Salmonella enteritidis ATCC 13076 and Staphylococcus aureus ATCC 6538, fungicidal properties were studied on fungi Candida albicans. All cultures were standardized to 109 CFU / cm3. Results. It is established that as a result of the conducted researches at studying preparation “Sukhodez” antimicrobic properties, it had high efficiency concerning action on strains of both gram-positive and gram-negative bacteria. It had a detrimental effect on bacteria when applied to concrete at an exposure of 1 hour with a rate of 75 mg per 1 m2, and had the same effect as when applied to a wooden surface, where it expressed antimicrobial action at a rate of 50 mg/m2 after 4 hours. When determining the fungicidal properties, it was found that when exposed to 5 hours, the preparation “Sukhodez” had an effect on study contaminated objects with a rate of 50 mg/m2, and with a rate of 75 mg/m2 inhibited the growth of fungi for 1 hour. Conclusions. It was found that the most sensitive to the preparation “Sukhodez” were the culture of Salmonella enteritidis – at a rate of 25 g / m2 and action on concrete and wooden surfaces, the disinfectant inhibited the growth of the culture when exposed to 5 hours, and at a rate of 50 g / m2 – for 2 years. Staphylococcus aureus was the most stable bacterial culture, so at a rate of 50 g / m2 on concrete and wood surfaces, bacterial growth was inhibited for 4 and 5 hours, respectively. At the same time, in the study of fungicidal properties, “Sukhodez” showed a fungicidal effect on Candida albicans when applied to a concrete surface at a rate of 50 mg / m2 for 3 hours, when applied to a wooden surface – for 4 hours. In general, at a rate of 75 g / m2, “Sukhodez” has an instant bactericidal and fungicidal effect on bacteria and fungi applied to concrete and wooden surface

RESEARCH ON THE BEHAVIOUR OF MDF DOOR FRAMES AFTER IMMERSION IN WATER

The paper presents the results of the researchwork performed on the behaviour of the door framesmade of laminated MDF (cherry and walnut foil) onimmersion in water. The MDF boards were purchasedfrom two different suppliers. The immersion wasmade untill the critical limit was reached, when thedimensional characteristics of the frame came out ofthe field of tolerances. The dimensionalmeasurements, together with the mass weights wereperformed at different times, finaly achieving differentcritical limits for the two types of materials. The finalconclusion of the research showed that, after a periodof 24 hours of immersion in water, both materialshave suffered major dimensional changes thataffected the use of the door frame. The paperprovides information on the relative and absolutevariations of sizes and mass, including percentagechanges

Isolation, identification and Analysis of Drug Resistance of Salmonella Pul-lorum

The article provides data on the isolation and identification of the pathogen S. Pullorum from pathological material of chickens. With further study of tinctorial, cultural-morphological and biological properties of the pathogen. The susceptibility of Salmonella pullorum to broad-spectrum antibacterial drugs such as cephalosporins and carbopenems was further studied to determine the drug of choice for improving treatment and prevention of avian bacterial diseases. In order to diagnose Salmonella pullorum (S. Pullorum) diarrhea accurately and analyze its drug resistance. In this study, the pathogen of a chicken suspected of S. Pullorum was isolation, PCR amplification and drug sensitivity analysis of the pathogen from in chicken farm in Xinxiang, north China. The results showed that the bacteria strain was diagnosed as S. Pullorum base on isolation and identification, Gram staining and biochemical identification of the bacteria. Antibacterial drugs sensitivity test confirmed that the bacteria was sensitive to ceftiofur, ceftriaxone, meropenem and kanamycin, and the effect of sensitive antibiotics was obvious in clinical treatment. Altogether, the present experiment revealed a detailed measure for S. Pullorum prevention and control and that achieved good clinical results, which laid a fundamental information for farmers and veterinary workers on eradication of S. Pullorum

Effect of feeding of chelated zinc form on security, productivity and slaughter parameters of broilers

The results of the effect of feeding the chelated form of Zinc on the safety, productivity and slaughter parameters of broiler chickens are presented. The aim of this study was to determine the safety, productivity and slaughter parameters of broilers on the background of feeding chelated form of Zinc. Materials and methods. Experimental studies were conducted during 2020 on broiler chickens cross Cobb-500 in the vivarium of the Veterinary Faculty of Sumy National Agrarian University (Ukraine), divided into two groups on the principle of analogues (control and experimental) of 50 broilers each. The source of zinc for chickens in the control group was their sulfates. Chelated forms of Zinc were used for broiler chickens of the experimental group. All groups of broiler chickens were administered the compound with compound feed in a dose that corresponded to the daily requirement of the bird in this trace element. The duration of the experiment is 42 days. The growth and development of broilers were evaluated on the basis of determining the relevant zootechnical indicators. Live weight of birds was determined by individual weighing on scales of the VNC type with an accuracy of ±1 g at the age of 7, 14, 21, 28, 35 and 42 days. Both scientific and economic experiments also determined the preservation of livestock - daily by the number of culled and dead birds. Feed consumption was recorded daily, for each week of cultivation and for the entire period of the experiment. At the end of the experiment, the cost of feed per 1 kg of live gained weight was calculated. Results. According to the results of the conducted researches, it was established that feeding of the chelated form of Zinc probably increased both the average daily gain and the total live weight of broiler chickens at different age periods of rearing. The live weight of chickens at the beginning of the experiment was almost the same, but at 7 days of age it tended to increase in chickens of the experimental group, although a significant difference between groups in this indicator was not found. It was found that the weight of uneviscerated, semi-eviscerated and eviscerated chicken in poultry of the experimental group was greater than similar indicators in the young animals of the control group by 9.3, 9.2, and 9.8 %, and this difference was significant (p<0.01). The yield of semi-eviscerated carcasses was higher in ones of the experimental group (84.19 %), and the difference was significant (p<0.05). Conclusions. It was found that the live weight of chickens of the experimental group (consumption of feed with a chelated form of Zinc) prevailed at 7 days of age by 1.1 g; on the 14th day – on 22.1 g (p<0.01); at 21 days – 35.4 g (p<0.01); at 28 days – 94.5 g (p<0.05); at 35 days – 133.6 g (p<0.01); at 42 days – at 218.9 g (p<0.05). Preservation of young animals in the experimental group, which used chelated compounds of Zinc, was higher and amounted to 96 %, compared with the control – 92 %. By age, feed costs per unit weight gain were lower in the experiment, respectively: 0.05; 0.08; 0.12; 0.06; 0.07 and 0.05 kg, compared to the control group. Growing broiler chickens on compound feed with a chelated form of Zinc by 9.8 % (p<0.01) increases the weight of the eviscerated carcass

The effects of mismatches on hybridization in DNA microarrays: determination of nearest neighbor parameters

Quantifying interactions in DNA microarrays is of central importance for a better understanding of their functioning. Hybridization thermodynamics for nucleic acid strands in aqueous solution can be described by the so-called nearest-neighbor model, which estimates the hybridization free energy of a given sequence as a sum of dinucleotide terms. Compared with its solution counterparts, hybridization in DNA microarrays may be hindered due to the presence of a solid surface and of a high density of DNA strands. We present here a study aimed at the determination of hybridization free energies in DNA microarrays. Experiments are performed on custom Agilent slides. The solution contains a single oligonucleotide. The microarray contains spots with a perfect matching complementary sequence and other spots with one or two mismatches: in total 1006 different probe spots, each replicated 15 times per microarray. The free energy parameters are directly fitted from microarray data. The experiments demonstrate a clear correlation between hybridization free energies in the microarray and in solution. The experiments are fully consistent with the Langmuir model at low intensities, but show a clear deviation at intermediate (non-saturating) intensities. These results provide new interesting insights for the quantification of molecular interactions in DNA microarrays.Comment: 31 pages, 5 figure

Orthorectification Tests Continued... Formosat-2 Orthorectification Of Formosat-2 Data for use in The Common Agricultural Policy Control with Remote Sensing Programme

FORMOSAT-2 (NSPO, Taiwan) was launched on 21st of May, 2004. FORMOSAT-2 was programmed as Very High Resolution backup sensor in the CwRS campaign for the first time in 2006 [Ref 1]. Acquisition success rate has been high since it was introduced due to its high (daily) revisit capacity, but difficulties were initially encountered to reach the required location accuracy in production of orthorectified imagery. This resulted in a 1st study (2006) where FORMOSAT-2 imagery over Sofia (BG) was assessed; 4 software suites were tested on this image with low off-nadir viewing angle [Ref 2]. Results were promising, demonstrating that it is possible to perform good orthorectification using standard software packages reaching results inside the CwRS requirements for such imagery (location accuracy preliminary set to 3.5m RMSE1D). In this 2nd study (2007) the aim has been to assess the effect of large off-nadir angles on the accuracy of the orthorectification, and to define the optimal number of GCPs to be used when orthorectifying FORMOSAT-2 images on a routine basis. Results of orthocorrection of 4 images of different off-nadir angles (along/across angles), over 2 sites in France and Bulgaria, using 4 different sw suites (PCI, ERDAS Imagine, PRODIGEO, and Keystone SIPOrtho,) and with varying number of GCPs are discussed. The results are consistent with theoretical expectations; x error increases when across angle (roll) increases, the y error increases when along angle (pitch) increases. Basically the accuracy of 5m RMSE1D is reached with all tested softwares, the 3.5m RMSE1D accuracy may be reached if limits are placed on the acquisition angles. Concerning the GCP requirement a total of minimum 10 GCPs should be used: four GCPs spread in the corners of the scenes, the others evenly distributed, and clearly visible.JRC.G.3-Agricultur

Adjuvant effects of a sequence-engineered mRNA vaccine: translational profiling demonstrates similar human and murine innate response

Additional file 1: Fig. S1. Components and assembly of the PTE module for innate responses. (A) Top panel—in vivo monocytes continuously emigrate from the blood into peripheral tissues with a half-life in the blood of ~1 day. Bottom panel: In vitro generation of cytokine derived DCs from monocytes involves the addition of IL-4 and GM-CSF and culture for 7–11 days. (B) Schematic of the components of a MIMIC-PTE module. In the 3D PTE, differentiation occurs in hours to about 2 days triggered by migration into and out of (reverse migration) through the endothelium: a process reminiscent of the movement of cells from tissues into lymphatic vessels