34 research outputs found
Biohydrogen production by Thermoanaerobacterium thermosaccharolyticum KKU-ED1: Culture conditions optimization using mixed xylose/arabinose as substrate
Background: Biological hydrogen production by microorganisms can be
divided into two main categories i.e. photosynthetic organisms that
produce hydrogen using light as energy source and anaerobic bacteria
that produce hydrogen via dark fermentation. Dark fermentative hydrogen
production by anaerobic bacteria has the advantages of a higher HPR
without illumination and of the capability to convert various kinds of
substrate. Results: Thermophilic hydrogen producer was isolated from
elephant dung and identified as Thermoanaerobacterium
thermosaccharolyticum KKU-ED1 by 16S rRNA gene analysis, which was
further used to produce hydrogen from mixed pentose sugar i.e.,
xylose/arabinose. The optimum conditions for hydrogen production from
mixed xylose/arabinose by KKU-ED1 were a 1:1 xylose/arabinose mixture
at the total concentration of 5 g/L, initial pH of 6.5 and temperature
of 55\ubaC. Under the optimum conditions, hydrogen from sugar derived
from acid-hydrolyzed sugarcane bagasse at a reducing sugar
concentration were achieved. Soluble metabolite product (SMP) was
predominantly acetic acid indicating the acetate-type fermentation.
Conclusions: The strain KKU-ED1 appeared to be a suitable candidate for
thermophilic fermentative hydrogen production from hemicellulosic
fraction of lignocellulosic materials due to its ability to use various
types of carbon sources