Non-cystic fibrosis bronchiectasis: review and recent advances

Bronchiectasis is an abnormal dilatation of bronchi and bronchioles associated with repeated cycles of airway infection and inflammation. This review will focus on non-cystic fibrosis bronchiectasis in children, with regard to etiology, diagnosis, treatment options, and recent advances

Isolation and Characterization of Microsatellite Loci in the Asian Rice Gall Midge (Orseolia oryzae) (Diptera: Cecidomyiidae)

Microsatellite loci were isolated from the genomic DNA of the Asian rice gall midge, Orseolia oryzae (Wood-Mason) using a hybridization capture approach. A total of 90 non-redundant primer pairs, representing unique loci, were designed. These simple sequence repeat (SSR) markers represented di (72%), tri (15.3%), and complex repeats (12.7%). Three biotypes of gall midge (20 individuals for each biotype) were screened using these SSRs. The results revealed that 15 loci were hyper variable and showed polymorphism among different biotypes of this pest. The number of alleles ranged from two to 11 and expected heterozygosity was above 0.5. Inheritance studies with three markers (observed to be polymorphic between sexes) revealed sex linked inheritance of two SSRs (Oosat55 and Oosat59) and autosomal inheritance of one marker (Oosat43). These markers will prove to be a useful tool to devise strategies for integrated pest management and in the study of biotype evolution in this important rice pest

Durability Tests of a Fiber Optic Corrosion Sensor

Steel corrosion is a major cause of degradation in reinforced concrete structures, and there is a need to develop cost-effective methods to detect the initiation of corrosion in such structures. This paper presents a low cost, easy to use fiber optic corrosion sensor for practical application. Thin iron film is deposited on the end surface of a cleaved optical fiber by sputtering. When light is sent into the fiber, most of it is reflected by the coating. If the surrounding environment is corrosive, the film is corroded and the intensity of the reflected signal drops significantly. In previous work, the sensing principle was verified by various experiments in laboratory and a packaging method was introduced. In this paper, the method of multiplexing several sensors by optical time domain reflectometer (OTDR) and optical splitter is introduced, together with the interpretation of OTDR results. The practical applicability of the proposed sensors is demonstrated in a three-year field trial with the sensors installed in an aggressive marine environment. The durability of the sensor against chemical degradation and physical degradation is also verified by accelerated life test and freeze-thaw cycling test, respectively

Carbonation of surface protected concrete

Concrete structures are exposed to carbonation that may cause rapid decay, shortening their service life and raising maintenance and repair costs. Carbonation lowers the alkalinity of the concrete depassivating the steel reinforcement. Two limit states can be identified with regard to service life. The first limit state ends when the steel is depassivated. The second limit state is based on cracking of the concrete cover due to oxides generated during corrosion. The service life includes a certain propagation period of corrosion during which the cross-sectional area of steel is progressively decreased, the bond between steel and concrete is reduced and the effective cross-sectional area of concrete is diminished due to spalling of the cover. Surface treatment is commonly used to improve the resistance of concrete to carbonation. A barrier is formed retarding the carbonation reactions in the interior of the concrete. In this study surface protected concretes were compared with non-protected concretes. The used surface protected concretes presented generally lower carbonation diffusion coefficients than the non-protected concretes. The use of epoxy resin showed better protection than the use of acrylic and siloxane resins. The composition of the concretes is an important factor affecting the diffusion of carbonation. The influence of the water–cement ratio was very important. The carbonation diffusion coefficients increased with the water–cement ratio. The prescriptive methodology is not a guarantee to obtain the desired service lives of 50 or 100 years. The desired services lives were only obtained with the use of surface protection treatments

Serine Proteases-Like Genes in the Asian Rice Gall Midge Show Differential Expression in Compatible and Incompatible Interactions with Rice

The Asian rice gall midge, Orseolia oryzae (Wood-Mason), is a serious pest of rice. Investigations into the gall midge-rice interaction will unveil the underlying molecular mechanisms which, in turn, can be used as a tool to assist in developing suitable integrated pest management strategies. The insect gut is known to be involved in various physiological and biological processes including digestion, detoxification and interaction with the host. We have cloned and identified two genes, OoprotI and OoprotII, homologous to serine proteases with the conserved His87, Asp136 and Ser241 residues. OoProtI shared 52.26% identity with mosquito-type trypsin from Hessian fly whereas OoProtII showed 52.49% identity to complement component activated C1s from the Hessian fly. Quantitative real time PCR analysis revealed that both the genes were significantly upregulated in larvae feeding on resistant cultivar than in those feeding on susceptible cultivar. These results provide an opportunity to understand the gut physiology of the insect under compatible or incompatible interactions with the host. Phylogenetic analysis grouped these genes in the clade containing proteases of phytophagous insects away from hematophagous insects

Effect of nano and micro-silica on bond behaviour of steel and polypropylene fibres in high volume fly ash mortar

This paper presents the effects of nano silica (NS), micro silica (MS) and combined NS and MS on bond behaviour of steel and polypropylene (PP) fibres in high volume fly ash (HVFA) mortar. Three types of bend configuration of hook-end steel fibre commercially available are considered, while the PP fibre was crimped shape. Three different fly ash contents of 40%, 50% and 60% (by wt) as partial replacement of ordinary Portland cement (OPC) are considered in HVFA mortar, while a control mortar containing 100% OPC was also considered. The NS and the MS was added as 2% and 10% (by wt), respectively as partial replacement of OPC in HVFA mortar containing 40% fly ash. In the case of combined NS and MS, 2% NS and 10% MS was used as partial replacement of OPC in HVFA mortar. However, in the case of HVFA mortars containing 40% fly ash and different NS and MS, total OPC content of 60% was kept constant in all HVFA mixes containing NS, MS and NS + MS. This was considered to compare these mixes with HVFA mortar containing 40% fly ash. Results indicate that maximum pull-out force of both steel and PP fibres decreases with increase in fly ash contents in HVFA mortars at both 7 and 28 days.The addition of 2% NS and 10% MS showed almost similar improvement in the maximum pull-out force of steel and PP fibres at both ages in HVFA mortar containing 40% fly ash. The combined use of 2%NS + 10%MS also improved the maximum pull-out force and higher than 2% NS and 10% MS. The reduction in large capillary pores in HVFA mortars containing nano and micro silica observed in Mercury Intrusion Porosity test improved the bond of steel and PP fibres in those mortar due to formation of additional calcium silicate hydrate (C-S-H) gel is believed to be the reason behind this improvement. The maximum pull-out force also increased with increase in number of bends in the hook-end of steel fibre in all mortars in this study at both 7 and 28 days. Extra energy absorbed by the higher number of bends is the reason of such improvement in maximum pull-out force. However, in the case of absorbed energy mixed results are observed in the case of different number of bends in steel fibre ends. Good correlations also exist between the maximum pull-out forces of all three types of steel fibres with compressive strength of mortars showing strong influence on the bond behaviour

Inhalation characteristics of asthma patients, COPD patients and healthy volunteers with the Spiromax® and Turbuhaler® devices: a randomised, cross-over study.

BACKGROUND: Spiromax® is a novel dry-powder inhaler containing formulations of budesonide plus formoterol (BF). The device is intended to provide dose equivalence with enhanced user-friendliness compared to BF Turbuhaler® in asthma and chronic obstructive pulmonary disease (COPD). The present study was performed to compare inhalation parameters with empty versions of the two devices, and to investigate the effects of enhanced training designed to encourage faster inhalation. METHODS: This randomised, open-label, cross-over study included children with asthma (n = 23), adolescents with asthma (n = 27), adults with asthma (n = 50), adults with COPD (n = 50) and healthy adult volunteers (n = 50). Inhalation manoeuvres were recorded with each device after training with the patient information leaflet (PIL) and after enhanced training using an In-Check Dial device. RESULTS: After PIL training, peak inspiratory flow (PIF), maximum change in pressure (∆P) and the inhalation volume (IV) were significantly higher with Spiromax than with the Turbuhaler device (p values were at least <0.05 in all patient groups). After enhanced training, numerically or significantly higher values for PIF, ∆P, IV and acceleration remained with Spiromax versus Turbuhaler, except for ∆P in COPD patients. After PIL training, one adult asthma patient and one COPD patient inhaled <30 L/min through the Spiromax compared to one adult asthma patient and five COPD patients with the Turbuhaler. All patients achieved PIF values of at least 30 L/min after enhanced training. CONCLUSIONS: The two inhalers have similar resistance so inhalation flows and pressure changes would be expected to be similar. The higher flow-related values noted for Spiromax versus Turbuhaler after PIL training suggest that Spiromax might have human factor advantages in real-world use. After enhanced training, the flow-related differences between devices persisted; increased flow rates were achieved with both devices, and all patients achieved the minimal flow required for adequate drug delivery. Enhanced training could be useful, especially in COPD patients

Molecular Approaches for Insect Pest Management in Rice

AbstractThis chapter focuses on the progress made in using molecular tools in understanding resistance in rice to insect pests and breeding rice for multiple and durable insect resistance. Currently, molecular markers are being extensively used to tag, map, introgress, and clone plant resistance genes against gall midge, planthoppers, and leafhoppers. Studies on cloned insect resistance genes are leading to a better understanding of plant defense against insect pests under different feeding guilds. While marker-assisted breeding is successfully tackling problems in durable and multiple pest resistance in rice, genomics of plants and insects has identified RNAi-based gene silencing as an alternative approach for conferring insect resistance. The use of these techniques in rice is in the developmental stage, with the main focus on brown planthopper and yellow stem borer. CRISPR-based genome editing techniques for pest control in plants has just begun. Insect susceptibility genes (negative regulators of resistance genes) in plants are apt targets for this approach while gene drive in insect populations, as a tool to study rice-pest interactions, is another concept being tested. Transformation of crop plants with diverse insecticidal genes is a proven technology with potential for commercial success. Despite advances in the development and testing of transgenic rice for insect resistance, no insect-resistant rice cultivar is now being commercially cultivated. An array of molecular tools is being used to study insect-rice interactions at transcriptome, proteome, metabolome, mitogenome, and metagenome levels, especially with reference to BPH and gall midge, and such studies are uncovering new approaches for insect pest management and for understanding population genetics and phylogeography of rice pests. Thus, it is evident that the new knowledge being gained through these studies has provided us with new tools and information for facing future challenges. However, what is also evident is that our attempts to manage rice pests cannot be a one-time effort but must be a continuing one