Serosurvey for viruses associated with reproductive failure in newly introduced gilts and in multiparous sows in Belgian sow herds

A serosurvey for viruses associated with reproductive disorders was conducted in 25 conventional Belgian farms. Serum antibody titers for porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2 (PCV2), porcine parvovirus (PPV), porcine enteroviruses (PEV) and swine influenza viruses (SIV) were determined in gilts and sows. All the animals were seropositive for PCV2 and >95% were seropositive for all 4 embryopathogenic PEV serotypes. Consequently, special preventive measures appear to be unnecessary for these viruses. In I farm, non-vaccinated gilts were found to run a risk of developing PPV-induced reproductive disorders. Vaccination against PPV could exclude this risk. In 10 farms, gilts seronegative for one or more specific SIV subtypes were introduced into a herd that had previously been infected with the same subtypes. Vaccination of gilts against SIV may prevent reproductive disorders in gilts and respiratory problems in their offspring. In I farm, newly purchased gilts that were possibly shedding PRRSV were introduced into a PRRSV seronegative sow herd. Serological screening prior to purchase or vaccination of the sows could have resolved this dangerous situation

Absolute differential positronium-formation cross sections

The first absolute experimental determinations of the differential cross-sections for the formation of ground-state positronium are presented for He, Ar, H2 and CO2 near 0○. Results are compared with available theories. The ratio of the differential and integrated cross-sections for the targets exposes the higher propensity for forward-emission of positronium formed from He and H2

Exégèse et théologie de l’islam shi’ite

I. Le Coran des origines : le contexte. II. Le Coran des origines : ses supports matériels et sa rédaction comme œuvre scribale. III. La théologie initiale du Coran : un Dieu souverai

Replication of avian, human and swine influenza viruses in porcine respiratory explants and association with sialic acid distribution

<p>Abstract</p> <p>Background</p> <p>Throughout the history of human influenza pandemics, pigs have been considered the most likely "mixing vessel" for reassortment between human and avian influenza viruses (AIVs). However, the replication efficiencies of influenza viruses from various hosts, as well as the expression of sialic acid (Sia) receptor variants in the entire porcine respiratory tract have never been studied in detail. Therefore, we established porcine nasal, tracheal, bronchial and lung explants, which cover the entire porcine respiratory tract with maximal similarity to the <it>in vivo </it>situation. Subsequently, we assessed virus yields of three porcine, two human and six AIVs in these explants. Since our results on virus replication were in disagreement with the previously reported presence of putative avian virus receptors in the trachea, we additionally studied the distribution of sialic acid receptors by means of lectin histochemistry. Human (Siaα2-6Gal) and avian virus receptors (Siaα2-3Gal) were identified with <it>Sambucus Nigra </it>and <it>Maackia amurensis </it>lectins respectively.</p> <p>Results</p> <p>Compared to swine and human influenza viruses, replication of the AIVs was limited in all cultures but most strikingly in nasal and tracheal explants. Results of virus titrations were confirmed by quantification of infected cells using immunohistochemistry. By lectin histochemistry we found moderate to abundant expression of the human-like virus receptors in all explant systems but minimal binding of the lectins that identify avian-like receptors, especially in the nasal, tracheal and bronchial epithelium.</p> <p>Conclusions</p> <p>The species barrier that restricts the transmission of influenza viruses from one host to another remains preserved in our porcine respiratory explants. Therefore this system offers a valuable alternative to study virus and/or host properties required for adaptation or reassortment of influenza viruses. Our results indicate that, based on the expression of Sia receptors alone, the pig is unlikely to be a more appropriate mixing vessel for influenza viruses than humans. We conclude that too little is known on the exact mechanism and on predisposing factors for reassortment to assess the true role of the pig in the emergence of novel influenza viruses.</p

Cross-protection against European swine influenza viruses in the context of infection immunity against the 2009 pandemic H1N1 virus : studies in the pig model of influenza

Pigs are natural hosts for the same influenza virus subtypes as humans and are a valuable model for cross-protection studies with influenza. In this study, we have used the pig model to examine the extent of virological protection between a) the 2009 pandemic H1N1 (pH1N1) virus and three different European H1 swine influenza virus (SIV) lineages, and b) these H1 viruses and a European H3N2 SIV. Pigs were inoculated intranasally with representative strains of each virus lineage with 6- and 17-week intervals between H1 inoculations and between H1 and H3 inoculations, respectively. Virus titers in nasal swabs and/or tissues of the respiratory tract were determined after each inoculation. There was substantial though differing cross-protection between pH1N1 and other H1 viruses, which was directly correlated with the relatedness in the viral hemagglutinin (HA) and neuraminidase (NA) proteins. Cross-protection against H3N2 was almost complete in pigs with immunity against H1N2, but was weak in H1N1/pH1N1-immune pigs. In conclusion, infection with a live, wild type influenza virus may offer substantial cross-lineage protection against viruses of the same HA and/or NA subtype. True heterosubtypic protection, in contrast, appears to be minimal in natural influenza virus hosts. We discuss our findings in the light of the zoonotic and pandemic risks of SIVs

HD 41641: A classical δ\delta Sct-type pulsator with chemical signatures of an Ap star

Among the known groups of pulsating stars, $\delta$ Sct stars are one of the least understood. Theoretical models do not predict the oscillation frequencies that observations reveal. Complete asteroseismic studies are necessary to improve these models and better understand the internal structure of these targets. We study the $\delta$ Sct star HD 41641 with the ultimate goal of understanding its oscillation pattern. The target was simultaneously observed by the CoRoT space telescope and the HARPS high-resolution spectrograph. The photometric data set was analyzed with the software package PERIOD04, while FAMIAS was used to analyze the line profile variations. The method of spectrum synthesis was used for spectroscopically determining the fundamental atmospheric parameters and individual chemical abundances. A total of 90 different frequencies was identified and analyzed. An unambiguous identification of the azimuthal order of the surface geometry could only be provided for the dominant p-mode, which was found to be a nonradial prograde mode with m = +1. Using $T_\mathrm{eff}$ and $\log g$, we estimated the mass, radius, and evolutionary stage of HD 41641. We find HD 41641 to be a moderately rotating, slightly evolved $\delta$ Sct star with subsolar overall atmospheric metal content and unexpected chemical peculiarities. HD 41641 is a pure $\delta$ Sct pulsator with p-mode frequencies in the range from 10 d$^{-1}$ to 20 d$^{-1}$. This pulsating star presents chemical signatures of an Ap star and rotational modulation due to surface inhomogeneities, which we consider indirect evidence of the presence of a magnetic field.Comment: 11 pages, 11 figures, accepted for publication in A&

KIC 10080943: a binary star with two γ Doradus/δ Scuti hybrid pulsators. Analysis of the g modes

We use 4 yr of Kepler photometry to study the non-eclipsing spectroscopic binary KIC 10080943. We find both components to be γ Doradus/δ Scuti hybrids, which pulsate in both p and g modes. We present an analysis of the g modes, which is complicated by the fact that the two sets of l = 1 modes partially overlap in the frequency spectrum. Nevertheless, it is possible to disentangle them by identifying rotationally split doublets from one component and triplets from the other. The identification is helped by the presence of additive combina- tion frequencies in the spectrum that involve the doublets but not the triplets. The rotational splittings of the multiplets imply core rotation periods of about 11 and 7 d in the two stars. One of the stars also shows evidence of l = 2 modes