Investigating Antifungal Susceptibility in Candida Species With MALDI-TOF MS-Based Assays.

Half of invasive fungal infections lead to death. Amongst pathogenic fungi, the most widespread species belong to the Candida genus and vary in their susceptibility to antifungal drugs. The emergence of antifungal resistance has become a major clinical problem. Therefore, the definition of susceptibility patterns is crucial for the survival of patients and the monitoring of resistance epidemiology. Although, most routinely used methods of AntiFungal Susceptibility Testing (AFST) have reached their limits, the rediscovery of Matrix Associated Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) in the field of mycology provides a promising alternative for the study of antifungal resistance. MALDI-TOF MS is already used in mycology for fungal identification, which permits to highlight inherent antifungal resistance. However, the main concern of clinicians is the rise of acquired antifungal resistance and the time needed for their detection. For this purpose, MALDI-TOF MS has been shown to be an accurate tool for AFST, presenting numerous advantages in comparison to commonly used techniques. Finally, MALDI-TOF MS could be used directly to detect resistance mechanisms through typing. Consequently, MALDI-TOF MS offers new perspectives in the context of healthcare associated outbreaks of emerging multi-drug resistant fungi, such as C. auris. As a proof of concept, we will illustrate the current and future benefits in using and adapting MALDI-TOF MS-based assays to define the susceptibility pattern of C. auris, by species identification, AFST, and typing

Preliminary petrological inferences on the high-grade metamorphic rocks exhumed by the South Rif Thrust, Prerif, Northern Morocco

The exhumation of deep crustal rocks along major shear zones is common, yet a highly debated subject, particularly when occurring during recent tectonic events. This is the case of the South Rif Thrust (SRT), a significant shear zone analogous to those described at the Betic Cordillera in Spain. The SRT separates two major geodynamic domains in Northern Morocco: a) the Prerif, to the North, mostly composed of Miocenic sedimentary units on top of a stratigraphic sequence continuously deposited since the Triassic; and b) the Western Meseta, to the South, mostly composed of Paleozoic metasedimentary units, correlated with the Iberian Variscan Belt [1]. Associated to the SRT, and exhumed by its activity, there is a dismembered and exotic high-grade metamorphic belt representative of the middle and lower crust. Also in this region, several thermo-mineral waters occur, whose deep circulation can be traced back to the SRT [2]. Detailed geological mapping, structural, stratigraphic and petrographic analyses on this dismembered and highly deformed exotic sequence reveal the presence of low- to high-grade metasediments (including migmatites and felsic granulites), but mostly high-grade metabasic and basic rocks, including amphibolites, mafic granulites and gabbros. Preliminary geothermobarometry in the mafic granulites provides an important characterization of the infra-crustal conditions of the pre-Alpine geodynamics and of the activity and exhumation along the SRT since the Miocene: a) the mafic granulites endured M1 metamorphic peak conditions of T = 1030 ºC at P = 8.5 kbar, which is consistent with typical conductive continental crust geothermal gradients (~30 ºC.km-1); b) M2 retrogression occurred by near isothermal decompression at T = 820 ºC and P = 3.5 kbar, implying an initial vertical uplift of >18 km of the granulite-facies rocks to very shallow levels; c) during this period, the geothermal gradient in the region surpassed 60 ºC.km-1; d) exhumation and retrogression continued by almost isobaric cooling at T < 750 ºC and P = 1.7 – 3.0 kbar with an M3 amphibolitization of the granulites after late water inflow. The overall metamorphic evolution of these deep crustal rocks is compatible with a clockwise P-T path, involving initial fast tectonic exhumation, followed by thermal readjustment to shallower levels. This is consistent with the currently observed geothermal gradients in the area (≤ 42 ºC.km-1) [2] which may still be a reflection of the events during the Miocene. These petrological constrains on the tectonic processes associated with the exhumation of this lower crust segment and the activity of the SRT during the closure of the Alboran Basin are key to understanding the circulation of deep hot waters, which are an important part of the economy of this region in Northern Morocco.publishe

Amerindian genetic ancestry and INDEL polymorphisms associated with susceptibility of childhood B-cell Leukemia in an admixed population from the Brazilian Amazon

AbstractAcute lymphoblastic leukemia (ALL) is a malignant tumor common in children. Studies of genetic susceptibility to cancer using biallelic insertion/deletion (INDEL) type polymorphisms associated with cancer development pathways may help to clarify etymology of ALL. In this study, we investigate the role of eight functional INDEL polymorphisms and influence of genetic ancestry to B-cell ALL susceptibility in children of Brazilian Amazon population, which has a high degree of inter-ethnic admixture. Ancestry analysis was estimated using a panel of 48 autosomal ancestry informative markers. 130 B-cell ALL patients and 125 healthy controls were included in this study. The odds ratios and 95% confidence intervals were adjusted for confounders. The results indicated an association between the investigated INDEL polymorphisms in CASP8 (rs3834129), CYP19A1 (rs11575899) e XRCC1 (rs3213239) genes in the development of B-cell ALL. The carriers of Insertion/Insertion (Ins/Ins) genotype of the polymorphism in CASP8 gene presented reduced chances of developing B-cell ALL (P=0.001; OR=0.353; 95% CI=0.192–0.651). The Deletion/Deletion (Del/Del) genotype of the polymorphism in CYP19A1 gene was associated to a lower chance of developing B-cell ALL (P=3.35×10−6; OR=0.121; 95% CI=0.050–0.295), while Del/Del genotype of the polymorphism in XRCC1 gene was associated to a higher chance of developing B-cell ALL (P=2.01×10−4; OR=6.559; 95% CI=2.433–17.681). We also found that Amerindian ancestry correlates with the risk of B-cell ALL. For each increase of 10% in the Amerindian ancestry results in 1.4-fold chances of developing B-cell ALL (OR=1.406; 95% IC=1.123–1.761), while each increase of 10% in the European ancestry presents a protection effect in the development of B-cell ALL (OR=0.666; 95% IC=0.536–0.827). The results suggest that genetic factors influence leukemogenesis and might be explored in the stratification of B-cell ALL risk in admixed populations

The revolving door : evidence from the United Kingdom, Germany, France, Spain, Belgium, Greece and Brazil

The following study analyses the academic background and careers of 175 members of Governments and Central Banks of seven countries (Belgium, Brazil, France, Germany, Greece, Spain and United Kingdom) for the years 1975 and 2015, in order to verify whether the “Revolving Door Theory” can be applied to these cases. After some research on the curricula vitae of the members of Governments and Central Banks, we found that, for instance, that more Government and Central Bank members studied abroad for the case of the UK and US than for the other countries. We also found that it is more common for Central Bank executive members to obtain PhDs than it is the case for Government members. Moreover, external promotions in the Central Banks in 1975 were quite relevant but no cases were registered for 2015; for Governments, the trend was the exact opposite, no external promotions in 1975 but many cases in 2015. While it is not possible to find irrefutable evidence to sustain the Revolving Door hypothesis, it is still possible to find recurrent patterns in different countries that may be explained by that theory. More expanded databases and a larger selection of countries is required for that analysis

A Influência De Crenças E Tabus Alimentares Na Amamentação

Cultural factors, such as food taboos, negatively influence the promotion of breastfeeding, resulting in early weaning. The aim of this study was to investigate differences in eating habits of a group of mothers in relation to breastfeeding. Cross-sectional study consisting of mothers of children under two years old enrolled in the Child Care Program of two Basic Health Units of Guarapuava-PR. One located in the peripheral region (US 1) and the other in the central area (US 2) to detect differences in eating habits of this population. The study included 96 mothers with mean age 25,11 years, of which 86.5% said they believed that some food would undermine breastfeeding. The main foods to be avoided, according to the mothers, were acidic foods, alcohol, chocolate and soft drinks. Mothers of units 1 and 2 have made mention consumption of foods derived from corn, and only two mentioned in the U.S. 2 beer consumption as food lactogenic black. No differences were found between the health U.S. 1 and U.S. 2. In relation to dietary restrictions were not found relevant data related to the taboos of the interviewees. Highlights the importance of orientation to promote breastfeeding and encourage mothers to solve any doubts, avoiding early weaning and continued breastfeeding until two years of age or older child.40222122

Morphology of Gambierdiscus excentricus (Dinophyceae) with emphasis on sulcal plates

Gambierdiscus excentricus is an epibenthic dinoflagellate able to produce ciguatoxin and maitotoxin-like compounds that are responsible for ciguatera fish poisoning. Morphological descriptions and molecular characterization of two G. excentricus strains isolated from Brazil and maintained in culture were provided. The most complete description of the morphology of the sulcal region of Gambierdiscus based on light and scanning electron microscopy was presented. The sulcal area morphology and nomenclature used by different authors to name the sulcal plates in Gambierdiscus were reviewed. Two small sulcal plates (S.m.a. and S.m.p.) were shown for the first time. Phylogenetic trees based on D1–D3 and D8–D10 large subunits of ribosomal RNA gene sequences showed that the strains of G. excentricus from Brazil clustered with strains of G. excentricus isolated from its type locality, the Canary Islands. Both phylogenetic trees reconstructed the same relationships among all the formally described Gambierdiscus species and Gambierdiscus sp. ribotype 2 and Gambierdiscus sp. type 2.Versión del editor2,080

Bond strength of lithium disilicate after cleaning methods of the remaining hydrofluoric acid

Different ceramic surface cleaning methods have been suggested after the acid conditioning. The aim was to evaluate the effect of different protocols used to remove the remaining hydrofluoric acid on the shear bond strength (SBS) between lithium disilicate and resin cement. Forty-four specimens of lithium disilicate (IPS e.max Press) were divided in 4 groups (n=11): group C (control, no treatment); group HF+S (5% hydrofluoric acid + silane); group HF+US+S (5% hydrofluoric acid + ultrasound cleaning + silane); group HF+PH+S (5% hydrofluoric acid + 37% phosphoric acid + silane). Scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) were performed to characterize the surface morphology. The SBS test was performed on the resin/ceramic interface, and the failure mode was characterized. SBS values were submitted to 1-way ANOVA and the Tukey test (?=.05). The relation between surface treatment and failure modes was analyzed using the chi-squared test (?=.05). The surface treatment type interfered in the shear strength (p<.001) and higher SBS values were observed for the groups HF+US+S (17.87 MPa) and HF+PH+S (16.37 MPa). The surface treatment did not influence the failure mode (p=.713). No fluorsilicate salts were observed after ultrasound cleaning. The utilization of ultrasound cleaning was an effective procedure to remove remaining fluorsilicate salts, promoting the highest SBS values

Periostin as a modulator of chronic cardiac remodeling after myocardial infarction

OBJECTIVE: After acute myocardial infarction, during the cardiac repair phase, periostin is released into the infarct and activates signaling pathways that are essential for the reparative process. However, the role of periostin in chronic cardiac remodeling after myocardial infarction remains to be elucidated. Therefore, the objective of this study was to investigate the relationship between tissue periostin and cardiac variables in the chronic cardiac remodeling induced by myocardial infarction. METHODS: Male Wistar rats were assigned to 2 groups: a simulated surgery group (SHAM; n = 8) and a myocardial infarction group (myocardial infarction; n = 13). After 3 months, morphological, functional and biochemical analyses were performed. The data are expressed as means±SD or medians (including the lower and upper quartiles). RESULTS: Myocardial infarctions induced increased left ventricular diastolic and systolic areas associated with a decreased fractional area change and a posterior wall shortening velocity. With regard to the extracellular matrix variables, the myocardial infarction group presented with higher values of periostin and types I and III collagen and higher interstitial collagen volume fractions and myocardial hydroxyproline concentrations. In addition, periostin was positively correlated with type III collagen levels (r = 0.673, p = 0.029) and diastolic (r = 0.678, p = 0.036) and systolic (r = 0.795, p = 0.006) left ventricular areas. Considering the relationship between periostin and the cardiac function variables, periostin was inversely correlated with both the fractional area change (r = -0.783, p = 0.008) and the posterior wall shortening velocity (r = -0.767, p = 0.012). CONCLUSIONS: Periostin might be a modulator of deleterious cardiac remodeling in the chronic phase after myocardial infarction in rats

"Plantar pro gasto": a importância do autoconsumo entre famílias de agricultores do Rio Grande do Sul.

O artigo discute a valoração e importância da produção para o autoconsumo na reprodução social das unidades familiares e caracteriza os alimentos autoconsumidos. Vale-se da pesquisa "Agricultura Familiar, Desenvolvimento Local e Pluriatividade" (UFRGS/UFPel/ CNPq-2003) que propiciou a formação de um banco de dados com informações sobre a dinâmica da agricultura familiar em quatro regiões distintas da geografia gaúcha, suas fontes e tipos de renda, entre estas o autoconsumo. Trazer este debate significa retomar um tema pouco discutido até então, e que, embora marginalizado ou considerado sem importância, desenvolve importante papel como renda não monetária, fortalece a segurança alimentar e adentra esferas da sociabilidade e identidade social. Além da introdução, apresenta-se o papel do autoconsumo na agricultura familiar, o cálculo da produção para o autoconsumo, discussão dos objetivos e resultados, e considerações finais. Os resultados demonstram que a produção para o autoconsumo é uma estratégia recorrente pelas unidades familiares e se diferencia de acordo com a dinâmica da agricultura familiar. Diferença esta expressa em valores relativos (%) e no número de estabelecimentos pertencentes a estratos diferenciados de autoconsumo, e pouco nos tipos de alimentos produzidos para este fim, observando-se uma homogeneidade dos hábitos alimentares