9 research outputs found
Developmental Stage, Phenotype, and Migration Distinguish Naive- and Effector/Memory-like CD4+ Regulatory T Cells
- Author
- Publication venue
- The Rockefeller University Press
- Publication date
- Field of study
Regulatory T cells (Tregs) fulfill a central role in immune regulation. We reported previously that the integrin αEÎČ7 discriminates distinct subsets of murine CD4+ regulatory T cells. Use of this marker has now helped to unravel a fundamental dichotomy among regulatory T cells. αEâCD25+ cells expressed L-selectin and CCR7, enabling recirculation through lymphoid tissues. In contrast, αE-positive subsets (CD25+ and CD25â) displayed an effector/memory phenotype expressing high levels of E/P-selectinâbinding ligands, multiple adhesion molecules as well as receptors for inflammatory chemokines, allowing efficient migration into inflamed sites. Accordingly, αE-expressing cells were found to be the most potent suppressors of inflammatory processes in disease models such as antigen-induced arthritis
Minimal Essential Human Factor VIII Alterations Enhance Secretion and Gene Therapy Efficiency
- Author
- Booth Carmen J.
- Cao Wenjing
- de la Rosa Maurus
- Dong Biao
- Firrman Jenni A.
- Hoellriegl Werner
- Horling Franziska
- Klugmann Matthias
- Konkle Barbara
- Lengler Johannes
- Li Dong
- Miao Carol
- Moore Andrea R.
- Reipert Birgit M.
- Roberts Sean A.
- Rottensteiner Hanspeter
- Scheiflinger Friedrich
- Wang Qizhao
- Wei Hongying
- Wu Wenman
- Xiao Weidong
- Publication venue
- 'Elsevier BV'
- Publication date
- 22/10/2020
- Field of study
One important limitation for achieving therapeutic expression of human factor VIII (FVIII) in hemophilia A gene therapy is inefficient secretion of the FVIII protein. Substitution of five amino acids in the A1 domain of human FVIII with the corresponding porcine FVIII residues generated a secretion-enhanced human FVIII variant termed B-domain-deleted (BDD)-FVIII-X5 that resulted in 8-fold higher FVIII activity levels in the supernatant of an in vitro cell-based assay system than seen with unmodified human BDD-FVIII. Analysis of purified recombinant BDD-FVIII-X5 and BDD-FVIII revealed similar specific activities for both proteins, indicating that the effect of the X5 alteration is confined to increased FVIII secretion. Intravenous delivery in FVIII-deficient mice of liver-targeted adeno-associated virus (AAV) vectors designed to express BDD-FVIII-X5 or BDD-FVIII achieved substantially higher plasma FVIII activity levels for BDD-FVIII-X5, even when highly efficient codon-optimized F8 nucleotide sequences were employed. A comprehensive immunogenicity assessment using in vitro stimulation assays and various in vivo preclinical models of hemophilia A demonstrated that the BDD-FVIII-X5 variant does not exhibit an increased immunogenicity risk compared to BDD-FVIII. In conclusion, BDD-FVIII-X5 is an effective FVIII variant molecule that can be further developed for use in gene- and protein-based therapeutics for patients with hemophilia A
Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries
- Author
- Abad-Motos A.
- Abbas A.M.
- Abbas M.
- Abbasi M.
- Abbott T.
- Abdalaal I.
- Abdel-Galil K.
- Abdelemam R.
- Abdelgawad A.
- Abdelkarim M.
- Abdelkhalek M.
- Abdelmageed A.
- Abdelmawgoud S.
- Abdelmohsen S.
- Abdelrahman A.S.
- Abdelrahman M.
- Abdelwahab K.
- Abdou H.
- Abdulai D.R.
- Abdulkhaleq M.
- Abdullah B.
- Abdulsalam K.
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- Abdur-Rahman L.
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- Abdussalam H.O.
- Abebrese J.T.
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- Publication venue
- Oxford University Press (OUP)
- Publication date
- 01/07/2023
- Field of study
Background
Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres.
Methods
This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and lowâmiddle-income countries.
Results
In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of âsingle-useâ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for lowâmiddle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia.
Conclusion
This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both highâ and lowâmiddleâincome countries
Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries
- Author
- Abad-Motos Ane
- Abbas Ahmed M.
- Abbas Mostafa
- Abbasi Mujeeb
- Abbott Tom
- Abd Karim Muhammad Fairuz Shah
- Abdalaal Ibrahim
- Abdel-Galil Khalid
- Abdelemam Rania
- Abdelgawad Abdalrahman
- Abdelkarim Maryam
- Abdelkhalek Mohamed
- Abdelmageed Abdelfatah
- Abdelmawgoud Sherif
- Abdelmohsen Sarah
- Abdelrahman Ahmed Saber
- Abdelrahman Mostafa
- Abdelwahab Khaled
- Abdou Hossam
- Abdulai Darling Ramatu
- Abdulkhaleq Marwa
- Abdullah Bahiyah
- Abdulsalam Khalifa
- Abdulsalam Shuaib
- Abdur-Rahman Lukman
- Abdus-Salam Rukiyat
- Abdussalam Hameedat Opeyemi
- Abebrese John Tabiri
- Abeldaño Ariel
- Abou-Khalil Jad
- Abou-Taleb Hisham
- Abozid Anwaar
- Abozied Hesham
- Abrate Alberto
- Abu-Zaid Ahmed
- Abubakar Mohammed Kabir
- Abud Burçin
- Abuzaid Mohammed
- Adam Albushra Altayeb
- Adamina Michel
- Adamou Harissou
- Adamu Auwal
- Adamu Kabir Musa
- Adanu Kekeli
- Adebara Idowu
- Adebola Oluwaseyi
- Ademuyiwa Adesoji
- Adeniran Abiodun
- Adepiti Akinfolarin
- Aderogba Adeleke Akeem
- Adesunkanmi Abdulhafiz
- Adeyeye Ademola
- Adisa Adewale
- Adjeso Theophilus Justus Kofi
- Ads Adel Mohamed
- Ads Alaa Mohamed
- Adu-Aryee Nii Armah
- Adumah Collins
- Afzal Irrum
- Agapov Mikhail
- Agarwal Gaurav
- Agarwal Ravi
- Agbeko Anita Eseenam
- Agbeno Evans Kofi
- Agbonrofo Peter
- Agboola Oluseyi
- Aggarwal Gaurav
- Aghadi Ifeanyi
- Aghtarafi Natasha
- Agrawal Amit
- Agresta Ferdinando
- Aguado HĂ©ctor J.
- Aguilera-Arevalo Maria-Lorena
- Aherne Thomas
- Ahmad Aline
- Ahmad Maleeha
- Ahmad Misbahu
- Ahmad Sarwat
- Ahmad Shahzaib
- Ahmad Shoaib
- Ahmed Hayat Tarig Abdelhafiz
- Ahmed Khalid
- Ahmed Sharwany
- Ahmed Shima
- Ahmed Usama
- Aisuodionoe-Shadrach Oseremen
- Aitken Sarah
- Ajenifuja Kayode
- Ajiya Abdulrazak
- Akeel Nouf
- Akin Emrah
- Akinajo Opeyemi
- Akinyemi Tosin
- Akrida Ioanna
- Aktokmakyan Talar Vartanoglu
- Akwaisah Ayoub
- Al Bettar Osama
- Al-Badawi Ismail A.
- Al-Juaifari Maytham
- Al-Khyatt Waleed
- Al-Mallah Abdullah
- Al-Nusairi Ahmed
- Al-Saadi Nina
- Al-Sayaghi Khaled
- Al-Shaiji Tariq
- Al-Shehari Mohammed
- Al-Touny Aiman
- Al-Touny Shimaa A.
- Aladawi Omar
- Aladna Abdallah
- Alali Mahmoud
- Alam Mahmood
- Alam Mohammed Shadrul
- Alarabi Rehab
- Alawneh Yazan
- Alayande Barnabas
- Albanese Erminia
- Albayadi Eslam
- Albdour Maram
- Alconchel Felipe
- Alderazi Amer
- Alegbeleye Bamidele
- Alekberli Tural
- Alemu Misganaw Alemu Adimass
- Alexander Philip
- Alfieri Alex
- Algahtany Khaled
- Algarni Kamal
- Alhamad Ameen
- Alhamid Mohammad
- Alharmi Rawan A. Rahman
- Alharthi Mohammed
- Alhazmi Barrag
- Ali Mohamed Shafi Mahboob
- Ali Stephen
- Alibrahim Hidar
- Aljaaly Hattan
- Aljaiuossi Anas
- Aljanadi Firas
- Aljiffry Murad
- Aljirdabi Noof
- Alkahlan Mohammed
- Alkhalifa Elmustafa
- Alkhatib Sondos
- Alkhatieb Maram
- Allan Anna
- Almarshad Felwa
- Almgla Naser
- Almhimid Ali
- Almiqlash Bushray
- Almoshantaf Mohammad Badr
- Almutairi Abdulmjeed
- Alnsour Abed Alfattah Mahmoud
- Alobied Majd
- Alomar Osama
- Alonso JesĂșs AarĂłn MartĂnez
- Alonso Manuel Diez
- Alonso Nestor Fabian Pedraza
- Alonso Veronica
- Alqallaf Abdullah
- Alqaseer Asma
- Alrayes Bourhan
- Alsefri Maryam
- Alser Osaid
- Alsharif Mohammed
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- Altaf Abdulmalik
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- Altouny Ahmad
- Altun Demet
- Alvarez Natalia
- Alvarez-Lozada Luis Adrian
- Alvi Abdul
- Alwael Athari
- Alwash Mohammed
- Alyami Ziyad
- Alyousef Mohammed
- Alzahrani Saud
- Alzerwi Nasser
- Amin Shivang
- Amir Mohammed
- Ammar Ahmed Siddique
- Ammendola Michele
- Amores Remedios Revilla
- Amrayev Sultan
- Anastasiadou Sofia
- Ancans Guntis
- Anderson Caroline
- Andreani Lorenzo
- Andreotti Dario
- Andreuccetti Jacopo
- Andrianakis Alexandros
- Andro Christophe
- Anesi Alexandre
- Angamuthu Stephen
- Angeles Martina Aida
- Angelico Roberta
- Angelis Fragkiskos
- Angelou Dimitrios
- Angelou Kyveli
- Angulo Victor Pinto
- Anjum Arfa
- Anjum Muhammad Ayyub
- Annicchiarico Alfredo
- Anoldo Pietro
- Ansaloni Luca
- Anthoulakis Christos
- Antonelli Amedeo
- Anwar Mohamed
- Anwar Sumadi Lukman
- Anwer Mariyah
- Anyanwu Lofty-John
- Apostolou Christos
- Apostolou Konstantinos
- Appiah-Kubi Adu
- Aprea Giovanni
- Arbab Hana
- Archer James
- Ardura Francisco
- Aremu Muyiwa
- Arif Tahera
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- Armijos Daniel
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- Atis Gokhan
- Atkins William
- Atkinson Kate
- Attwood Joseph
- Auerkari Aino
- Augestad Knut Magne
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- Aujayeb Avinash
- Avella Pasquale
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- Bahassan Omar Mohammed
- Bahrami-Hessari Michael
- Baia Marco
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- Bailey Karen
- Baili Efstratia
- Bains Lovenish
- Baiocchi Gian Luca
- Baiocchi Glauco
- Baiyewu Lateef Ayodele
- Bajaj Ankur
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- Balasubramanian Ishwarya
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- Balogun Simon
- Baloyiannis Ioannis
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- Croghan Stefanie M.
- Cross George W. V.
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- Cucinella Giuseppe
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- Demetriades Andreas K
- Demetriou Charis
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- El Assaad Hadi
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- El-Kassas Mohamed
- Elagili Ahmed
- Elahi Zain
- Elbadawy Merihan A.
- Elbahnasawy Mohamed
- Elbe Peter
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- Elliot R.
- Elnour Moheyaldien Ahmed Elamin
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- Fadel Zahir T
- Fahim Margo Medhat Fouad
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- Fayad Elsayed A.
- Federer Simon
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- Feeney Kaylem
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- Florian Ioan-Alexandru
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- Fowler George
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- Gil-Moreno Antonio
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- Giordano Vincenzo
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- Glasbey James
- Glass Nina
- Glowka Tim R.
- Gmati Aimen
- Goel Neha
- Goh Su Kah
- Goicea Raluca
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- Gollarte Ana SĂĄnchez
- Gomez Eduardo Garcia-Loarte
- Gomez-Fernandez Hugo
- Gomez-Rosado Juan-Carlos
- Gonzalez Agustin Rodriguez
- Gonzalez Lourdes Elorduy
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- Gracia Isabel
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- Guerrera Francesco
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- Guillen Jose Ramon Oliver
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- Gupta Amit
- Gupta Anand
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- Gurung Binay
- GuzmĂĄn David Santos
- Gyamfi Frank Enoch
- GĂĄlis Branislav
- GĂłmez Mercedes Estaire
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- Habeeb Olufemi
- Habeebullah Alaa
- Hadaya Joseph
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- Haidopoulos Dimitrios
- Hailu Samuel
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- Hamdan Khairul Hazim
- Hamdan Qusai
- Hameed Bm Zeeshan
- Hamid Hytham K. S.
- Hamida Baha Ben
- Hamill James
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- Hannan Md. Jafrul
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- Hardcastle Timothy
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- Hasan Hayat Ben
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- Hassanin Mohamed Abdelghafor
- Hassouneh Ala
- Hayati Firdaus
- Hayne Dickon
- Haynes Alex
- Hecker Andreas
- Hegde Siddhi
- Heider Julia
- Heindl Ludwig Maximilian
- Hendriks Eduard
- Henein Christin
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- Heriot Alexander
- Herman Frima
- Hermosa Jose MarĂa GonzĂĄlez
- Herron Jonathan
- Heta Saimir
- Higgins Mark
- Hillier Roxane
- Hing Caroline
- Hing Jun Xian
- Hirji Sameer
- Ho Ken Weixing
- Hoang Alan
- Hodges Owen
- Hodgetts James Michael
- Hodgson Russell
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- Hooker Robert
- Horesh Nir
- Horsfall Hugo Layard
- Horsnell Jonathan
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- Howse Louise
- Hristova Kalina
- Huatuco René Manuel Palacios
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- Iacob Giulio
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- Inayat Farwa
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- Ingabire J. C. Allen
- Ingallinella Sara
- Ioannidis Argyrios
- Iplikçi Ayberk
- Ippoliti Simona
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- Isaza-Restrepo Andres
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- Islam Shahnoor
- Ismail Abdulaziz
- Ismail Lamiese
- Ismail Nizar
- Issa Fadi
- Issahalq Mohammed Duah
- Issaka Adamu
- Iturralde Jose Luis Fadil
- Iwasaki Masae
- Iyengar Karthikeyan
- Jach Robert
- Jack Laura
- Jafarov Ibrahim
- Janjua Muhammad Haris
- Jansen Shirley
- Jansen Yanina
- Jardinez Christiaan
- Javanmard-Emamghissi Hannah
- Jawad Monir
- Jawad Zaynab
- Jayarajah Umesh
- Jayawardane Asanka
- Jelovac Drago
- Jenkinson Michael
- Jiang Serena
- Jibreel Mohammed
- Jichi Tarik
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- John David
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- José Felipe
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- José Pedro
- Jotic Ana
- Joviliano Edwaldo Edner
- Juara HernĂĄndez
- Juloski Jovan
- Jung Josephine
- Kadiri Innih
- Kajal Kamal
- Kakotkin Victor
- Kalayarasan Raja
- Kale Sachin
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- Kalin Murat
- Kalyva Nektaria
- Kaman Lileswar
- Kamarajah Sivesh Kathir
- Kanatas Anastasios
- Kandevani Naser Yousefzadeh
- Kantor Elie
- Kapetanakis Emmanouil
- Kapetanios Georgios
- Kaplan Lewis
- Kaplan Mehmet
- Kaplowitz Jeremy
- Kapur Karan
- Kara Yasin
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- Karakullukcu Haluk Kerim
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- Karar Shoura
- Karimian Faramarz
- Karout Lina
- Karthigeyan Madhivanan
- Kaselas Christos
- Kassab Mohamad B.
- Kassam Al-Faraaz
- Katechia Devvrat
- Katorkin Sergey
- Katsaros Ioannis
- Katsiaras Lykoyrgos
- Katsura Morihiro
- Kauppila Joonas H
- Kaur Amanjot
- Kausar Nabila
- Kavalakat Alfie
- Kavanagh Marcel MarjanoviÄ
- Kazan Ozgur
- Kealey Joshua
- Kebede Ephrem
- Kedwany Ahmed
- Kelly Michael
- Ken-Amoah Sebastian
- Kenington Cleo
- Kennerton A.S.
- Kent Alistair J
- Keramati Mohammad Reza
- Kethy Fagnon
- Keçi Laura
- Khalifa Ahmad
- Khaliq Tanwir
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- Khobragade Krunal
- Khokhar Muhammad Imran
- Kinnair Anthony
- Kirac Iva
- Kirchweger Patrick
- Kirfi Abdullahi Musa
- Kirk Alan
- Kirkan Emre Furkan
- Kirmani Bilal
- Kishore Kunal
- Kisielewski MichaĆ
- Kisser Ulrich
- Klatte Tobias
- Klimovskij Michail
- Knight Stephen Richard
- Knowles Brett
- KocataĆ Ali
- Kocher Gregor Jan
- Kochiyama Tsukasa
- Koh Cherry
- Koh Frederick
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- Kolias Angelos
- Kolli Venkata Satish
- Koneru Anish
- Konjevoda Suzana
- Konney Anna
- Konrads Christian
- Konsten Joop
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- Korkolis Dimitrios
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- Kowalski Luiz Paulo
- Kowark Ana
- KoĆĄir Jurij AleĆĄ
- Krawczyk Wiktor
- Kriem Eslam
- Krishnamoorthy Vijay
- Kroon Hidde M.
- Kruse Philippe
- Kryzauskas Marius
- Kucuk Gultekin Ozan
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- Kulimbet Mukhtar
- Kulis Tomislav
- Kulkarni Amruta
- Kumar Calvin
- Kumar Navin
- Kumar Sandip
- Kumar Vishal
- Kuppler Christopher
- Kurawa Musbahu
- Kuriyama Akira
- Kusuma Venkata Ramana Murthy
- Kusunoki Munenori
- Kutuzov Vladislav
- Kykalos Stylianos
- Köglberger Paul
- Köksoy ĂlkĂŒ Ceren
- Königsrainer Ingmar
- Labib Peter
- Laborde Mario Mella
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- Ladipo-Ajayi Oluwaseun
- Laeke Tsegazeab
- LaganĂ Antonio Simone
- Laird Alexander
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- Lebe Moritz
- Lechuga Daniel Garay
- Ledda Virginia
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- Lohmann Tibor Karl
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- Mabood Waleed
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- Maguire Patrick J.
- MaheÄiÄ Tina TomiÄ
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- Mallmann Christoph
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- Martins Paulo N.
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- Myriokefalitaki Eva
- MĂ©ndez Patricia Serrano
- Nabil Fatma
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- Nacion Aeris Jane D.
- Nadi Khaldoun
- Naegele Felix
- Naga Hani
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- Nahm Christopher
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- Nakano Luis
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- Narvaez-Rojas Alexis Rafael
- Nassar Mahmoud A.
- Nasser Sara
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- Niewiera Marc
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- Nitschke Christine
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- Noel Colin
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- Ntalouka Maria P.
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- Othman Ahmad Faidzal
- Othman Amani Alsayd Abdulsalam
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- Perra Teresa
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- Persad Amit
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- Pessaux Patrick
- Petagna Lorenzo
- Petersen Maria Laura
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- Petrucciani NiccolĂČ
- Petruzzi Gerardo
- Pettigrove K.L.
- Phillips Jonathan
- Picardi Biagio
- Picchetto Andrea
- Picciariello Arcangelo
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- Pinkney Thomas
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- Piozzi Guglielmo NiccolĂČ
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- Pirozzi Brunella Maria
- Pirrello Roberto
- Pirzada Muhammad Taqi
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- Pockney Peter
- Podda Mauro
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- Prada Carlos
- Pradhan Akhilesh
- Pramesh C.S.
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- Prieto Mikel
- PrijoviÄ NebojĆĄa
- Prodromidou Anastasia
- Proud David
- Prucher Gian Marco
- Prusty Lydia
- Pu Jingya Jane
- Pucher Philip
- Punjabi Prakash P
- Puri Ajay
- Purich Kieran
- PĂ©rez Beatriz GĂłmez
- PĂ©rez-BertĂłlez Sonia
- Qader Kawthar
- Qin Kirby R.
- Quadri Oladeji
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- Querney Jill
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- Quzli Ahmad
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- Rauf Umme Habiba Abdul
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- Rezende Ricardo Fernandes
- Rhomberg Thomas
- Ribeiro JoĂŁo
- Riccetti Camilla
- Richard H.
- Rickard Jennifer
- RincĂłn Natalia Andrea Rivera
- Ripollés-Melchor Javier
- Risaliti Matteo
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- Rodrigues Sara Castanheira
- Rodriguez H. Alejandro
- Rodriguez Magdiel
- Rodriguez-Lopez Mario
- RodrĂguez Lorena Varela
- Rokohl Alexander Christopher
- Roland Christina L
- Rolinger Jens
- Romano Angela
- Romano Maurizio
- Romanzi Andrea
- Romera Jorge Sanz
- Romero Patricia
- Ronellenfitsch Ulrich
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- Rowe Christopher
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- Ruiz-MarĂn Miguel
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- Russo Iris Shari
- Ryalino Christopher
- Ryan James
- Ryan Ăanna
- Saad Rema
- Saaid Rahmah
- Sabbagh Abdulrahman J.
- Sabella Danielle
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- Sacdalan Marie Dione
- Sachdev Gaurav
- Sachdeva Ashwin
- Sacras Maria LuĂs
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- Shah Vinay
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- Shiban Ehab
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- Shirazi Ahmed
- Shiwani Haaris
- Shmukler Daniel
- Shokor Muntaser Abu
- Sholadoye Tunde T.
- Shoukrie Shoukrie I. M.
- Shrestha Rashmit
- Siboe Mark
- Sica Giuseppe
- Siddig Esra
- Siddiqui M. Tayyab
- Sidiropoulos Theodoros
- Sievers Denise
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- Silvestri Vania
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- Siragusa Leandro
- Sivaprakasam Rajesh
- Skipworth Richard Je
- Slavchev Mihail
- Sleem Adham
- Smati Ismail
- Smelt Jeremy
- Smith A.
- Smolle-Juettner Freyja-Maria
- Sodhai Vivek
- Sofi Khalid
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- Soibiharry Adaiah
- Sokolov Manol
- Solaini Leonardo
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- Sole-Sedeno Josep M.
- Soljic Martina
- Solli Piergiorgio
- Soon Wai Cheong
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- Sotiropoulou Maria
- Sotudeh Mohadeseh
- Souadka Amine
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- Sparavigna Marco
- Spartalis Michael
- Spellar Kayleigh
- Spiers Harry V. M.
- Spina Alfio
- Spriano Giuseppe
- Squiers John
- Stabler Robert
- Stamatakis Emmanouil
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- Stather Philip
- Stevens Sean
- Stevenson Andrew
- Stewart Grant D.
- Stijns Jasper
- Stogowski Piotr Tomasz
- Stoleriu Mircea Gabriel
- Stoyanov Vladislav
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- Su Yu-Xiong
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- Subramani Yamini
- Subramaniam Sentilnathan
- Sue-Chue-Lam Colin
- Suer Muhammed Salih
- Sulaiman Bilal
- Sultania Mahesh
- Sundarraju Shalini
- Sungurtekin Hulya
- Suresh Sreelakshmi
- Surya Ilham Utama
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- Syllaios Athanasios
- Symeonidis Dimitrios
- SzabĂłmihĂĄlyovĂĄ KatarĂna
- Szakmany Tamas
- Szatmary Peter
- Sztipits TamĂĄs
- Szyluk Karol
- SĂĄ-Marta Eduarda
- Sånchez Alberto Cabañero
- SĂĄnchez Rosa
- Sånchez-Guillén Luis
- SĂŒleyman Marlen
- Tadic Boris
- Taha Anas
- Taha-Mehlitz Stephanie
- Tailor Bhavesh
- Taj Eldeen Samaher Taj Eldeen Hassan
- Takeda Flavio Roberto
- Takkenberg Marijn
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- Talwar Rishi
- Tamayo Beatriz Villota
- Tamburello Sara
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- Tammo Ămer
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- Tampaki Ekaterini Christina
- Tampakis Athanasios
- Tan Chih Ying
- Tan Shawn Jia Hwang
- Tanal Mert
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- Tarek Ahmed
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- Tashkandi Wail
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- Tatsis Dimitris
- Tawalbeh Ra'Fat
- Tawiah Augustine
- Tebha Sameer
- Techapongsatorn Suphakarn
- Teixeira Pedro
- Tejero-Pintor Francisco J.
- Tellez Karla Susana Martin
- Tellez Luis Garcia-Sancho
- Thaha Mohamed Adhnan
- Thakar Sumit
- Thaw May Htoo
- Theivendran Kanthan
- Thekkinkattil Dinesh
- Theochari Nikoletta
- Theodoropoulos George
- Theophilidou Elena
- Thereska Dariel
- Thiruchandran Gajendiran
- Thomakos Nikolaos
- Thomas Bradley
- Thomas Jerin Jose
- Thuduvage Vasanthika
- Thumbadoo Ruben
- Tian Sarah
- Tidjane Anisse
- Tirloni Luca
- Toia Francesca
- Tokidis Evripidis
- Tokocin Merve
- Tolani Musliu Adetola
- Toma Elena Adelina
- Tomasicchio Giovanni
- Tomlinson James
- Tonini Valeria
- Torre Beatrice
- Torregrosa Cristina
- Torun Bahar Canbay
- Tottman R.
- Tousidonis Manuel
- Trabulsi Nora
- Tranca Sebastian
- Trebol Jacobo
- Trejo-Avila Mario
- Triantafyllou Alexandra
- Triantafyllou Tania
- Trifunovski Aleksandar
- Tripathi Manjul
- Tripathi Prashant
- Tripathy Sujit
- Trivik-Barrientos Felipe
- Trostchansky Ivan
- Trulson Alexander
- Trungu Sokol
- Truss Adam
- Tsakiridis Ioannis
- Tsakiridis Nikolaos
- Tsiantoula Paraskevi
- Tsironis Dimitrios
- Tsolakidis Alexandros
- Tsoulfas Georgios
- Tucker Olga
- Tuech Jean Jacques
- Turan Alparslan
- Turi Stefano
- Turna Akif
- Turrado-Rodriguez Victor
- Turri Giulia
- Tutino Roberta
- Tuyishime Eugene
- Tzelves Lazaros
- Tzima Ioanna
- Tzimas George
- Ubiali Paolo
- Ubom Akaninyene Eseme
- Ugwu Aloy Okechukwu
- Ugwu Ebere
- Ugwu Ifeanyichukwu
- Ul Huda Maria Noor
- Ulas Ali Bilal
- Uldry Emilie
- Ulgur Hanife Seyda
- Ulkucu Attila
- Ulman Elif Akbas
- Ulman Hilmican
- UlutaĆ Mehmet EĆref
- Underwood Kirk
- Unsworth Richard
- Uprak Tevfik Kivilcim
- Urriza Pablo Vallés
- Usman Muhammad
- Usman Mustapha Ibrahim
- Vaassen Lauretta
- Vaccara Vincenzo La
- Vagena Xanthoula
- Vagge Aldo
- Vailas Michail
- Valadez Andrea Estefania Cueto
- Valadez Tania Abigail Cueto
- Valanci Sofia
- Valdespino Cristina Puentes
- Valeanu Liana
- Valioulis Ioannis
- Valladares Sergio ChĂĄvez
- Vallve-Bernal Marc
- Van Acker Jill
- Van Den Eynde Jef
- Van Goor Harry
- Van Vliet Helen
- Van Wyk Naomi Natasha
- Vanni Camilla
- Vas Aurelia
- Vasarhelyi Edward
- Vaschetti Roberto
- Vassiliu Panteleimon
- Vatansever Dogan
- Vatish Jamie
- Vaughan-Shaw Peter G.
- Vaysburg Dennis
- Veglia Antonella
- Velten Markus
- VelĂĄzquez Maria Jose Martinez
- Venkatappa Sunil Kumar
- Venketeswaran Meenakshi
- Venskutonis Donatas
- Verbic Milena Senica
- Verdi Daunia
- Vergara-Fernandez Omar
- Verhoeff Kevin
- Veroux Massimiliano
- Verras Georgios-Ioannis
- Verre Luigi
- Vida Lucian
- Vidya Raghavan
- Viglietta Edoardo
- Vijay Vardhini
- Vijgen Guy
- VilarĂł Blanca Capdevila
- Villanueva Julio
- Vilz Tim O.
- Vincent Elizabeth
- Vinci Danilo
- Viola Simona
- Violante Tommaso
- Vishnoi Jeewan Ram
- Vissapragada Ravi
- Vivancos Laura Guillamon
- Vives Josep Maria Muñoz
- Vizzielli Giuseppe
- Vohra Lubna
- Vokshi Ismail
- Vosinakis Christos
- Våsquez Javier Alberto Gutiérrez
- VĂĄzquez Gil
- VĂĄzquez Irene Ortega
- VÇcÇraĆu Andrei Bogdan
- Wade Ryckie
- Wafi Omar
- Wagner Gabriela
- Wallis Christopher
- Wanjara Samuel
- Waqar Shahzad Hussain
- Warden Claire
- Warner Christian
- Wasim D.
- Wason Shaun
- Wasserman Isaac
- Watcyn-Jones Tom
- Waterson Ben
- Watson David
- Watson Matthew
- Weber Clemens
- Wheatstone P.
- Wheeler James
- Wheldon Luke
- White Michelle
- White Stuart
- White William
- Wickramasinghe Dakshitha
- Widmer Lukas Werner
- Wiedemann Dominik
- Wiesinger Clemens Georg
- Wijekoon Naveen
- Wijenayake Wasantha
- Wijeyaratne Manuk
- Wijeysundera Duminda
- Wiles Matthew
- Wilkin Richard
- Wilkins Alex
- Wilkins James
- William Tinka George
- Williams Omolara Modupe
- Williams Shekinah
- Windsor John
- Winnand Philipp
- Wittmann Maria
- Wolde Amanuel
- Wolfenden John W. D.
- Wong Benjamin Ngie Xiong
- Wong Enoch
- Wong Evan G.
- Wong George Kwok Chu
- Wong Ho Ying Flora
- Wong Kai Yuen
- Wormald Justin
- Wright Deborah
- Wright Joshua
- Wu Fiona
- Wurm Markus
- Xenaki Sofia
- Yadev Ip
- Yahaya Ahmad Shuib
- Yakubu Mustapha
- Yang Wah
- Yanik Ăzge
- Yanowsky-Reyes Guillermo
- Yassin Abubaker
- Yates Elizabeth
- Yebes Alvaro
- Yenli Edwin Mwintiereh Ta-Ang
- Yeoh Tien
- Yeung Justin
- Yiallourou Anneza
- Yigit Banu
- Yildirim Asif
- Yim Guang
- Yiu Andrew
- Yonekura Hiroshi
- Yotsov Tsanko
- Younis Muhammad Umar
- Yunus Alif
- Yusefi Hossein
- Zabaleta Jon
- Zabkiewicz Catheriner
- Zaccaria Giulia
- Zacharia Bincy
- Zafar Syed Nabeel
- Zago Mauro
- Zaheen Zakia
- Zahid Zahra
- Zaimis Tilemachos
- Zakaria Andee Dzulkarnaen
- Zambon Martina
- Zamora Lindsey
- Zampogna Biagio
- Zancana Giuseppa
- Zani Augusto
- Zanin Luca
- Zanini Nicola
- Zapardiel Ignacio
- Zapata Carlos Shiraishi
- Zarif Pierre
- ZarĂĄnd Attila
- Zehnder Philipp
- Zenger Serkan
- Zenha HorĂĄcio
- Zhu Michael Z. L.
- Ziemann Sebastian
- Zimak Danijela Mrazovac
- Zimak Zoran
- Zivkovic Dragana
- Zizzo Maurizio
- Zucker Benjamin
- Zwain Yasir
- Zyskowski Michael
- Ăvila Clara Castro
- ĂlgĂ„ Andreas
- Ăakici Mehmet Ăaǧlar
- Ăelik Enes
- Ăelik Meryem Nur
- Ăzçay Necdet
- Ćevik HĂŒsnĂŒ
- Ć antak Goran
- Ć imko KristiĂĄn
- ĆœateckĂœ Jan
- Publication venue
- 'Oxford University Press (OUP)'
- Publication date
- 01/01/2023
- Field of study
Abstract
Background
Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres.
Methods
This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and lowâmiddle-income countries.
Results
In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of âsingle-useâ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for lowâmiddle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia.
Conclusion
This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both highâ and lowâmiddleâincome countries
Production of therapeutic levels of human FIX-R338L by engineered B cells using GMP-compatible medium
- Author
- Publication venue
- Elsevier
- Publication date
- 01/12/2023
- Field of study
B cells can differentiate into plasmablast and plasma cells, capable of producing antibodies for decades. Gene editing using zinc-finger nucleases (ZFN) enables the engineering of B cells capable of secreting sustained and high levels of therapeutic proteins. In this study, we established an advanced in vitro good manufacturing practice-compatible culturing system characterized by robust and consistent expansion rate, high viability, and efficient B cell differentiation. Using this process, an optimized B cell editing protocol was developed by combining ZFN/adeno-associated virus 6 technology to achieve site-specific insertion of the human factor IX R338L Padua into the silent TRAC locus. In vitro analysis revealed high levels of secreted human immunoglobulins and human factor IX-Padua. Following intravenous infusion in a mouse model, human plasma cells were detected in spleen and bone marrow, indicating successful and potentially long-term engraftment in vivo. Moreover, high levels of human immunoglobin and therapeutic levels of human factor IX-Padua were detected in mouse plasma, correlating with 15% of normal human factor IX activity. These data suggest that the proposed process promotes the production of functional and differentiated engineered B cells. In conclusion, this study represents an important step toward the development of a manufacturing platform for potential B cell-derived therapeutic products
CD4(+) T-cell epitopes associated with antibody responses after intravenously and subcutaneously applied human FVIII in humanized hemophilic E17 HLA-DRB1*1501 mice
- Author
- Aledort
- Ay
- Banchereau
- Bi
- Birgit M. Reipert
- Brigitte Binder
- Christian Lubich
- Corinna Hermann
- Das
- David C. Wraith
- de Groot
- Forsthuber
- Frentsch
- Germain
- Germain
- Gouw
- Gregersen
- Hammer
- Hans Peter Schwarz
- Hausl
- Hausl
- Hay
- Houston
- Hoyer
- Hu
- Jacquemin
- James
- James
- Katharina N. Steinitz
- Klein
- Kruisbeek
- Kumar
- Maria Schuster
- Mariuzza
- Markus Weiller
- Maurus de la Rosa
- Muchitsch
- Oldenburg
- Oldenburg
- Pasqual
- Pauline M. van Helden
- Pavlova
- Peng
- Pickl
- Pratt
- Rafi U. Ahmad
- Ragni
- Rajewsky
- Reding
- Reding
- Reding
- Reipert
- Sabine Unterthurner
- Sasgary
- Schellekens
- Sinigaglia
- Suri
- van Haren
- Vidovic
- Vinuesa
- von Boehmer
- Wight
- Woods
- Publication venue
- 'American Society of Hematology'
- Publication date
- 26/04/2012
- Field of study
Today it is generally accepted that B cells require cognate interactions with CD4(+) T cells to develop high-affinity antibodies against proteins. CD4(+) T cells recognize peptides (epitopes) presented by MHC class II molecules that are expressed on antigen-presenting cells. Structural features of both the MHC class II molecule and the peptide determine the specificity of CD4(+) T cells that can bind to the MHC class II-peptide complex. We used a new humanized hemophilic mouse model to identify FVIII peptides presented by HLA-DRB1*1501. This model carries a knock-out of all murine MHC class II molecules and expresses a chimeric murine-human MHC class II complex that contains the peptide-binding sites of the human HLA-DRB1*1501. When mice were treated with human FVIII, the proportion of mice that developed antibodies depended on the application route of FVIII and the activation state of the innate immune system. We identified 8 FVIII peptide regions that contained CD4(+) T-cell epitopes presented by HLA-DRB1*1501 to CD4(+) T cells during immune responses against FVIII. CD4(+) T-cell responses after intravenous and subcutaneous application of FVIII involved the same immunodominant FVIII epitopes. Interestingly, most of the 8 peptide regions contained promiscuous epitopes that bound to several different HLA-DR proteins in in vitro binding assays
Delayed colorectal cancer care during covid-19 pandemic (decor-19). Global perspective from an international survey
- Author
- Abary Ryan Rainiel A
- Abdelwahab Khaled M Egypt
- AbellĂĄn Antonio M Spain
- Abraham Ned Australia
- Achkasov Sergey I Russia
- Adamina Michel Switzerland
- Adamo Vincenzo Italy
- Agapov Mikhail Russia
- Agarwal Amit USA
- Aguado LĂłpez HĂ©ctor Spain
- Aguilar Werner Chile
- AguilarMartĂnez MarĂa Del Mar AM Spain
- Aguilera Asuncion Spain
- Ahmed Jamil UK
- Aiello Domenico Italy
- Akagi Tomonori Japan
- Akcakaya Adem Turkey
- Akhtar Khalid I Pakistan
- Akiba Ricardo Brazil
- Akrami Majid Iran
- Akyol Cihangir Turkey
- Al-Radjid Jamiri Philippines
- Alaamer Ohood H Saudi Arabia
- Alapati Kishore India
- Alasari Sami Saudi Arabia
- Albastaki Sara UAE
- Albergaria Diogo Portugal
- Alconchel Felipe Spain
- Alerta-Torre Andrea J Philippines
- Alex Ochsner Switzerland
- Alexander Herold Germany
- Alfaro Samuel El Salvador
- Ali Jehangir F Pakistan
- Aliyazicioglu Tolga Turkey
- Almgla Naser South Africa
- Alonsopoza Alfredo Spain
- Alselaim Nahar A Saudi Arabia
- Altaf Kiran K UK
- Althebaity Rasha Saudi Arabia
- Altinel Yuksel Turkey
- Altiparmak Basak Turkey
- Altomare Donato F Italy
- Alvandipour Mina Iran
- Alvarez-Gallego Mario Spain
- Alyami Mohammad Saudi Arabia
- Amado Sandra Portugal
- Amato Antonio Italy
- Amodio Pietro M Italy
- Ana-Maria MuÈinÄ Romania
- Andrade Luis Chile
- Andreas Kohler Switzerland
- Angelini Giulio Italy
- Angenete Eva Sweden
- Annicchiarico Alfredo Italy
- Antelo Galarza Renan Bolivia
- Anwer Mariyah Pakistan
- AparĂcio David J Portugal
- Arciniega Jose A Mexico
- Arenas Mara Belgium
- Arezzo Alberto Italy
- Argenio Giulio Italy
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- Arredondo Jorge Spain
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- Aumann Georg Germany
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- Ba Am Iraq
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- Clermonts Journal Pre-proof 39 Stefan HEM Netherlands
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- Daniels Ian R UK
- Dar Asif M India
- Davies Richard J UK
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- De Silva Kaluthanthiri Patabanadi VR Sri Lanka
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- De Wilt Johannes HW Netherlands
- De-LeĂłn-RendĂłn Jorge Luis Mexico
- Dean Phillip USA
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- Denoya Paula I USA
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- Doulias Triantafyllos A UK
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- Dunn Gary USA
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- Dworkin Michael United Kingdom
- Dybau Aleh Belarus
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- DâElia Antonio Italy
- Efetov Sergey K Russia
- Eisa Mohamed Egypt
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- Eliasrabelo Fernanda Brazil
- Ellis Tyler USA
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- Emmanuel Odet France
- Enomoto Masanobu Japan
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- Farmer Martin UK
- Farmer Russ USA
- Faul Eleanor M Ireland
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- Forerotorres Alexander Spain
- Forgan Timothy R South Africa
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- Gurjar Shashank UK
- Hainsworth Alison J UK
- Hall Nigel UK
- Hamed Hosam H Egypt
- Hammer Clare UK
- Hannon Rob Ireland
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- Italy
- Italy
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- Journal Pre-proof 42 Selemane Carlos Mozambique
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- Kelly Michael E Ireland
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- Konstantoudakis Georgios Cyprus
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- Lasala Alfred Philippines
- Lauretta Andrea Italy
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- Zucchella Martino Italy
- Zuhdy Mohammad
- Publication venue
- 'Elsevier BV'
- Publication date
- 01/01/2021
- Field of study
Background
The widespread nature of coronavirus disease 2019 (COVID-19) has been unprecedented. We sought to analyze its global impact with a survey on colorectal cancer (CRC) care during the pandemic.
Methods
The impact of COVID-19 on preoperative assessment, elective surgery, and postoperative management of CRC patients was explored by a 35-item survey, which was distributed worldwide to members of surgical societies with an interest in CRC care. Respondents were divided into two comparator groups: 1) âdelayâ group: CRC care affected by the pandemic; 2) âno delayâ group: unaltered CRC practice.
Results
A total of 1,051 respondents from 84 countries completed the survey. No substantial differences in demographics were found between the âdelayâ (745, 70.9%) and âno delayâ (306, 29.1%) groups. Suspension of multidisciplinary team meetings, staff members quarantined or relocated to COVID-19 units, units fully dedicated to COVID-19 care, personal protective equipment not readily available were factors significantly associated to delays in endoscopy, radiology, surgery, histopathology and prolonged chemoradiation therapy-to-surgery intervals. In the âdelayâ group, 48.9% of respondents reported a change in the initial surgical plan and 26.3% reported a shift from elective to urgent operations. Recovery of CRC care was associated with the status of the outbreak. Practicing in COVID-free units, no change in operative slots and staff members not relocated to COVID-19 units were statistically associated with unaltered CRC care in the âno delayâ group, while the geographical distribution was not.
Conclusions
Global changes in diagnostic and therapeutic CRC practices were evident. Changes were associated with differences in health-care delivery systems, hospitalâs preparedness, resources availability, and local COVID-19 prevalence rather than geographical factors. Strategic planning is required to optimize CRC care
Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries
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- Publication venue
- country:GB
- Publication date
- 01/07/2023
- Field of study
Background: Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods: This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was coprioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low-middle-income countries. Results: In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of 'single-use' consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low-middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion: This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high- and low-middle-income countries