La COVID-19 evidencia la necesidad de incrementar las competencias en economía de los estudiantes de veterinaria

The Covid-19 pandemic led to extreme control measures around the world aiming to halting the number of new infections. Non-essential activities closures and population confinement had an economic impact on the small ruminant sector, highlighting the need for veterinarians to have some skills to assess the economic impact of diseases on flocks. Firstly, this study analyzed the economic training received by the veterinary students at the Spanish faculties, also comparing it with the ones received by students of the agricultural engineering degree. Secondly, a survey in reference to the acquisition of this type of competences and its application for animal health was designed and applied for graduates and final-year veterinary students. The data showed that the ECTS taken to acquire the economic skills of veterinarians is less (3-6 ECTS in 5 years) than that of agricultural engineers (12 ECTS in 4 years). The results of the survey also showed that, although there are significant differences between graduates and students, both them are largely in agreement on the little training received to assess the impact of diseases, and on the need for an additional training after completing their studies. Therefore, these skills should be reinforced in the degree. Among the graduates, the opinion is similar, regardless of the years of professional experience.La pandemia Covid-19 ha motivado la adopción de medidas excepcionales en todo el mundo, a efectos de limitar los contagios y el colapso de los sistemas sanitarios. El cierre de comercios y otras actividades consideradas no esenciales, o las limitaciones al movimiento, ha generado un impacto económico en el sector de los pequeños rumiantes, poniendo de manifiesto la necesidad del profesional veterinario de disponer de las competencias necesarias para valorar económicamente el impacto de las enfermedades en los colectivos. Este trabajo analiza, en primer lugar, la formación en economía que reciben los estudiantes del Grado de Veterinaria en las diferentes facultades españolas, comparándola con la que reciben los estudiantes en el Grado de Ingeniería Agrícola. En segundo lugar, se ha diseñado una encuesta para egresados y estudiantes de último curso de veterinaria, en referencia a este tipo de competencias, su aplicación en la sanidad animal y su repercusión en el desempeño profesional. Los datos evidencian que el tiempo empleado para adquirir las competencias en economía de los veterinarios es menor (3-6 créditos en 5 años) que el que disponen los ingenieros agrícolas (12 créditos en 4 años). Los resultados de la encuesta revelan que, si bien se registran diferencias significativas cuantitativamente entre egresados y estudiantes, ambos grupos coinciden en la escasa formación recibida para valorar el impacto de las enfermedades en los colectivos, así como en la necesidad de formarse en aspectos de gestión económica una vez finalizados sus estudios de grado para el desempeño de su labor profesional, competencias que deberían ser reforzadas en el grado. Entre los egresados, la opinión es similar, independientemente de los años de desempeño profesional

Mycoplasma bovis in Spanish Cattle Herds: Two Groups of Multiresistant Isolates Predominate, with One Remaining Susceptible to Fluoroquinolones

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).This document is the Accepted version of a Published Work that appeared in final form in Pathogens. To access the final edited and published work see https://doi.org/10.3390/pathogens9070545Mycoplasma bovis is an important bovine pathogen causing pneumonia, mastitis, and arthritis and is responsible for major economic losses worldwide. In the absence of an efficient vaccine, control of M. bovis infections mainly relies on antimicrobial treatments, but resistance is reported in an increasing number of countries. To address the situation in Spain, M. bovis was searched in 436 samples collected from beef and dairy cattle (2016–2019) and 28% were positive. Single-locus typing using polC sequences further revealed that two subtypes ST2 and ST3, circulate in Spain both in beef and dairy cattle, regardless of the regions or the clinical signs. Monitoring of ST2 and ST3 isolates minimum inhibitory concentration (MIC) to a panel of antimicrobials revealed one major difference when using fluoroquinolones (FQL): ST2 is more susceptible than ST3. Accordingly, whole-genome sequencing (WGS) further identified mutations in the gyrA and parC regions, encoding quinolone resistance-determining regions (QRDR) only in ST3 isolates. This situation shows the capacity of ST3 to accumulate mutations in QRDR and might reflect the selective pressure imposed by the extensive use of these antimicrobials. MIC values and detection of mutations by WGS also showed that most Spanish isolates are resistant to macrolides, lincosamides, and tetracyclines. Valnemulin was the only one effective, at least in vitro, against both STs

Comparison of commercial enzyme-linked immunosorbent assays for diagnosis of contagious agalactia caused by Mycoplasma agalactiae

© 2022 A. Sánchez et al. This document is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in Journal of Veterinary Research. To access the final edited and published work see https://doi.org/10.2478/jvetres-2022-0010Introduction: Contagious agalactia (CA) is a disease affecting small ruminants with worldwide distribution and caused by several mycoplasmas, especially M. agalactiae. The main option for systematic diagnosis under monitoring control programmes is the enzyme-linked immunosorbent assay (ELISA) test. Material and Methods: This study was designed to appraise the performance of two commercial indirect ELISA tests using M. agalactiae p48 protein and one using total protein, for antibody detection in small ruminants after natural infection with different M. agalactiae strains. We carried out the test evaluation using sera of confirmed M. agalactiae-positive goats with clinical signs. In addition, test agreement was assessed by kappa between the three commercial ELISA tests. Results: All three ELISA tests showed high validity scores (Youden’s J: 72.9–84%). The sensitivity values for the P48 protein-based tests were 76.9% and 84.6%, and was 79% for the total protein-based test. The specificity of all tests was 100%. In addition, between the total protein-based ELISA test and the other two ELISA tests based on the P48 protein, the agreement was substantial (kappa: 0.762–0.763) and the agreement between the latter two tests was almost perfect (kappa: 0.93). Conclusion: The validity parameters for all tests allowed their application for diagnostic purposes in lactating goats excreting M. agalactiae in milk and presenting clinical signs

Antibacterial potential of commercial and wild lactic acid bacteria strains isolated from ovine and caprine raw milk against Mycoplasma agalactiae

© 2023 Toquet, Bataller, Gomis, Sánchez, Toledo-Perona, De la Fe, Corrales and Gómez-Martín. This is an open-access article distributed under the terms of the Creative Commons CC-BY license, http://creativecommons.org/licenses/by /4.0/ This document is the Published version of a Published Work that appeared in final form in Frontiers in Veterinary Science. To access the final edited and published work see https://doi.org/10.3389/fvets.2023.1197701Introduction: The complexity of fighting contagious agalactia (CA) has raised the necessity of alternative antimicrobial therapies, such as probiotics. Lactic acid bacteria (LAB) are present in the mammary gland of small ruminants and their antimicrobial effect have been previously described against species like Mycoplasma bovis but never against Mycoplasma agalactiae (Ma). This in vitro study aims to evaluate the antimicrobial activity against Ma of ovine and caprine LAB strains and a human commercial probiotic (L2) of Lactobacillus spp. Methods: A total of 63 possible LAB strains were isolated from nine ovine and caprine farms in Spain, three isolates (33B, 248D, and 120B) from the 63 strains were selected, based on their capacity to grow in a specific medium in vitro, for an in vitro experiment to assess their antimicrobial activity against Ma in Ultra High Temperature (UHT) processed goat milk (GM). A women commercial vaginal probiotic was also included in the study. The inoculum of L2 was prepared at a concentration of 3.24 × 108  CFU/mL and the average concentration of the inoculum of the wild LAB varied from 7.9 × 107 to 8.4 × 108  CFU/mL. Results: The commercial probiotic L2 significantly reduced the concentration of Ma to 0.000 log CFU/mL (p < 0.001), strain 33B reduced it from 7.185 to 1.279 log CFU/mL (p < 0.001), and 120B from 6.825 to 6.466 log CFU/mL (p < 0.05). Strain 248D presented a bacteriostatic effect in GM. Moreover, the three wild strains and the commercial probiotic produced a significative reduction of the pH (p < 0.001). Discussion: This is the first in vivo report of the antimicrobial potential of LAB strains against Ma and its interaction. Our results support possible future alternative strategies to antibiotic therapy, previously not contemplated, to fight CA in small ruminants. Further studies are necessary to elucidate the action mechanisms through which these LAB are able to inhibit Ma and to assess the safety of using these strains in possible in vivo studies

The addition of Lactobacillus spp. negatively affects Mycoplasma bovis viability in bovine cervical mucus

© The Author(s). 2020 Open Access This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). This document is the Published version of a Published Work that appeared in final form in BMC Veterinary Research. To access the final edited and published work see https://doi.org/10.1186/s12917-020-02454-9Background Mycoplasma bovis is an important pathogen for the cattle industry worldwide causing significant economic losses. Several transmission routes, including those related to reproduction, have been described. Indeed, the pathogen can colonize the female reproductive tract after artificial insemination (AI) with contaminated semen. Lactobacillus spp.-based probiotics have been used for vaginal dysbiosis treatment in women and cows although their role in controlling cervico-vaginal infections due to M. bovis is unknown. The objective of the present work is to assess the viability of M. bovis (PG45, NCTC 10131) in experimentally contaminated cervical mucus after the addition of Lactobacillus spp. at different concentrations as a competing agent and pH acidifier. Results The addition of probiotic at a concentration higher than 108 colony forming units (CFU/mL had a detrimental effect (P < 0.05) on mycoplasma viability in cervical mucus. This coincided with a significant LAB growth and an important decrease in pH from 8.4 to 5.6 (P < 0.05). However, after the addition of less concentrated probiotic, M. bovis survival was not affected and there was no significant LAB growth despite the drop of pH from 8.4 to 6.73 (P < 0.05). Conclusion The addition of concentrations higher than 108 CFU/mL of Lactobacillus spp. negatively affects M. bovis viability in bovine cervical mucus under in vitro conditions. Although the effect observed on the pathogen viability seems to be related to the pH decrease after LAB proliferation in cervical mucus, further studies are necessary to elucidate if other factors are implicated. Nevertheless, the administration of Lactobacillus spp.-based probiotics might be used in the future to control M. bovis proliferation in the cervico-vaginal tract of cows

The Addition of Lactobacillus spp., Enrofloxacin or Doxycycline Negatively Affects the Viability of Mycoplasma bovis in Diluted Bovine Semen

© The Authors. 2020. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). This document is the Published version of a Published Work that appeared in final form in Animals. To access the final edited and published work see https://doi.org/10.3390/ani10050837Mycoplasma bovis is an important etiologic agent of bovine mycoplasmosis in cattle. Different transmission routes have been described, including those related to reproduction. The presence of mycoplasma in semen has led to its appearance in infection-free areas through artificial insemination (AI). Semen was recently reported to be the initial source of two M. bovis mastitis outbreaks in two closed dairy herds in Finland. This questions the effectiveness of the antimicrobials currently used in semen extenders to control the pathogens in contaminated semen. They should be re-evaluated, or alternative measures to antimicrobials should be tested to obtain M. bovis-free semen. This in vitro study aimed to assess different strategies to reduce the risk of transmission of M. bovis through AI technologies. The viability of M. bovis (PG45, NCTC 10131) in bull semen diluted (DS) in a Tris-citrate-fructose solution was tested, after the addition of enrofloxacin, doxycycline or a Lactobacillus spp.-based probiotic. The data show the susceptibility of the pathogen to the addition of 0.125 μg/mL of enrofloxacin or 0.0625 μg/mL of doxycycline and to the addition of the probiotic at a concentration of 3.24 × 106 colony forming units (CFU)/mL or 3.24 × 108 CFU/mL in DS. The Tris-citrate-fructose medium negatively affected the viability of M. bovis, although this effect was lower than that observed after the addition of the probiotic and antimicrobials (p < 0.05). Our results may support new strategies for reducing the risk of M. bovis transmission through AI

The overlooked immune state in candidemia: A risk factor for mortality

Producción CientíficaLymphopenia has been related to increased mortality in septic patients. Nonetheless, the impact of lymphocyte count on candidemia mortality and prognosis has not been addressed. We conducted a retrospective study, including all admitted patients with candidemia from 2007 to 2016. We examined lymphocyte counts during the first 5 days following the diagnosis of candidemia. Multivariable logistic regression analysis was performed to determine the relationship between lymphocyte count and mortality. Classification and Regression Tree analysis was used to identify the best cut-off of lymphocyte count for mortality associated with candidemia. From 296 cases of candidemia, 115 died, (39.8% 30-day mortality). Low lymphocyte count was related to mortality and poor outcome (p < 0.001). Lymphocyte counts <0.703 × 109 cells/L at diagnosis (area under the curve (AUC)-ROC, 0.783 ± 0.042; 95% confidence interval (CI), 0.700–0.867, p < 0.001), and lymphocyte count <1.272 × 109 cells/L five days later (AUC-ROC, 0.791 ± 0.038; 95%CI, 0.716–0.866, p < 0.001) increased the odds of mortality five-fold (odds ratio (OR), 5.01; 95%CI, 2.39–10.93) at time of diagnosis, and three-fold (OR, 3.27; 95%CI, 1.24–8.62) by day 5, respectively. Low lymphocyte count is an independent predictor of mortality in patients with candidemia and might serve as a biomarker for predicting candidemia-associated mortality and poor outcome.Junta de Castilla y León (grant VA161G18

Evaluation of appendicitis risk prediction models in adults with suspected appendicitis

Background Appendicitis is the most common general surgical emergency worldwide, but its diagnosis remains challenging. The aim of this study was to determine whether existing risk prediction models can reliably identify patients presenting to hospital in the UK with acute right iliac fossa (RIF) pain who are at low risk of appendicitis. Methods A systematic search was completed to identify all existing appendicitis risk prediction models. Models were validated using UK data from an international prospective cohort study that captured consecutive patients aged 16–45 years presenting to hospital with acute RIF in March to June 2017. The main outcome was best achievable model specificity (proportion of patients who did not have appendicitis correctly classified as low risk) whilst maintaining a failure rate below 5 per cent (proportion of patients identified as low risk who actually had appendicitis). Results Some 5345 patients across 154 UK hospitals were identified, of which two‐thirds (3613 of 5345, 67·6 per cent) were women. Women were more than twice as likely to undergo surgery with removal of a histologically normal appendix (272 of 964, 28·2 per cent) than men (120 of 993, 12·1 per cent) (relative risk 2·33, 95 per cent c.i. 1·92 to 2·84; P < 0·001). Of 15 validated risk prediction models, the Adult Appendicitis Score performed best (cut‐off score 8 or less, specificity 63·1 per cent, failure rate 3·7 per cent). The Appendicitis Inflammatory Response Score performed best for men (cut‐off score 2 or less, specificity 24·7 per cent, failure rate 2·4 per cent). Conclusion Women in the UK had a disproportionate risk of admission without surgical intervention and had high rates of normal appendicectomy. Risk prediction models to support shared decision‐making by identifying adults in the UK at low risk of appendicitis were identified

COVID-19 highlights the need to increase health economics skills of veterinary students

La pandemia Covid-19 ha motivado la adopción de medidas excepcionales en todo el mundo, a efectos de limitar los contagios y el colapso de los sistemas sanitarios. El cierre de comercios y otras actividades consideradas no esenciales, o las limitaciones al movimiento, ha generado un impacto económico en el sector de los pequeños rumiantes, poniendo de manifiesto la necesidad del profesional veterinario de disponer de las competencias necesarias para valorar económicamente el impacto de las enfermedades en los colectivos. Este trabajo analiza, en primer lugar, la formación en economía que reciben los estudiantes del Grado de Veterinaria en las diferentes facultades españolas, comparándola con la que reciben los estudiantes en el Grado de Ingeniería Agrícola. En segundo lugar, se ha diseñado una encuesta para egresados y estudiantes de último curso de veterinaria, en referencia a este tipo de competencias, su aplicación en la sanidad animal y su repercusión en el desempeño profesional. Los datos evidencian que el tiempo empleado para adquirir las competencias en economía de los veterinarios es menor (3-6 créditos en 5 años) que el que disponen los ingenieros agrícolas (12 créditos en 4 años). Los resultados de la encuesta revelan que, si bien se registran diferencias significativas cuantitativamente entre egresados y estudiantes, ambos grupos coinciden en la escasa formación recibida para valorar el impacto de las enfermedades en los colectivos, así como en la necesidad de formarse en aspectos de gestión económica una vez finalizados sus estudios de grado para el desempeño de su labor profesional, competencias que deberían ser reforzadas en el grado. Entre los egresados, la opinión es similar, independientemente de los años de desempeño profesional.The Covid-19 pandemic led to extreme control measures around the world aiming to halting the number of new infections. Non-essential activities closures and population confinement had an economic impact on the small ruminant sector, highlighting the need for veterinarians to have some skills to assess the economic impact of diseases on flocks. Firstly, this study analyzed the economic training received by the veterinary students at the Spanish faculties, also comparing it with the ones received by students of the agricultural engineering degree. Secondly, a survey in reference to the acquisition of this type of competences and its application for animal health was designed and applied for graduates and final-year veterinary students. The data showed that the ECTS taken to acquire the economic skills of veterinarians is less (3-6 ECTS in 5 years) than that of agricultural engineers (12 ECTS in 4 years). The results of the survey also showed that, although there are significant differences between graduates and students, both them are largely in agreement on the little training received to assess the impact of diseases, and on the need for an additional training after completing their studies. Therefore, these skills should be reinforced in the degree. Among the graduates, the opinion is similar, regardless of the years of professional experience

RESPUESTA INMUNE HUMORAL EN CORDEROS Y CABRITOS INOCULADOS CON UNA VACUNA BIVALENTE FRENTE A AGALAXIA CONTAGIOSA

A combined vaccine against Mycoplasma agalactiae and Mycoplasma mycoides subsp. mycoides (large colony type) was developed using inactivated strains selected in previous characterization studies. Phenol was used as inactivating agent and aluminium hydroxide was added as adjuvant. The present study was designed to evaluate the specific humoral immune response to these two mycoplasma species shown by lambs and goat kids. One group of 15 Pelibuey lambs and one group of 8 Canary goat kids received two injections of vaccine at the ages 45 and 70 days and were monitored until they were 120 days old. Antibody titres were determined by indirect ELISA. Lambs showed lower antibody levels (expressed as ODs) to both antigens than goat kids althought significantly differences were only registered to M. agalactiae antigen (PUna vacuna polivalente frente a Mycoplasma agalactiae and Mycoplasma mycoides subsp. mycoides (large colony) fue elaborada utilizando cepas inactivadas seleccionadas en base a previos estudios de caracterización de las mismas. El fenol se utilizó como inactivante mientras que como adyuvante se utilizó el hidróxido de aluminio. El trabajo evaluó la respuesta immune humoral inducida en un grupo de 15 corderos Pelibuey y 8 cabritos de la raza canaria majorera, los cuales recibieron 2 dosis de vacuna a los 45 y 70 días de edad, siendo monitorizados hasta los 4 meses de vida. Los títulos de anticuerpos observados por ELISA indirecto muestran títulos menores en el grupo de corderos que en el grupo de cabritos frente a ambos antígenos, siendo estas diferencias estadísticamente significativas sólo frente a M. agalactiae