S100B in Guillain-Barre syndrome.

BR J ANAESTH. 2006 JAN;96(1):141-2

Stem cell impairment at the host–microbiota interface in colorectal cancer

Colorectal cancer (CRC) initiation is believed to result from the conversion of normal intestinal stem cells (ISCs) into cancer stem cells (CSCs), also known as tumor-initiating cells (TICs). Hence, CRC evolves through the multiple acquisition of well-established genetic and epigenetic alterations with an adenoma–carcinoma sequence progression. Unlike other stem cells elsewhere in the body, ISCs cohabit with the intestinal microbiota, which consists of a diverse community of microorganisms, including bacteria, fungi, and viruses. The gut microbiota communicates closely with ISCs and mounting evidence suggests that there is significant crosstalk between host and microbiota at the ISC niche level. Metagenomic analyses have demonstrated that the host– microbiota mutually beneficial symbiosis existing under physiologic conditions is lost during a state of pathological microbial imbalance due to the alteration of microbiota composition (dysbiosis) and/or the genetic susceptibility of the host. The complex interaction between CRC and microbiota is at the forefront of the current CRC research, and there is growing attention on a possible role of the gut microbiome in the pathogenesis of CRC through ISC niche impairment. Here we primarily review the most recent findings on the molecular mechanism underlying the complex interplay between gut microbiota and ISCs, revealing a possible key role of microbiota in the aberrant reprogramming of CSCs in the initiation of CRC. We also discuss recent advances in OMICS approaches and single-cell analyses to explore the relationship between gut microbiota and ISC/CSC niche biology leading to a desirable implementation of the current precision medicine approaches

Status of the Cylindical-GEM project for the KLOE-2 Inner Tracker

The status of the R&D on the Cylindrical-GEM (CGEM) detector foreseen as Inner Tracker for KLOE-2, the upgrade of the KLOE experiment at the DAFNE phi-factory, will be presented. The R&D includes several activities: i) the construction and complete characterization of the full-size CGEM prototype, equipped with 650 microns pitch 1-D longitudinal strips; ii) the study of the 2-D readout with XV patterned strips and operation in magnetic field (up to 1.5T), performed with small planar prototypes in a dedicated test at the H4-SPS beam facility; iii) the characterization of the single-mask GEM technology for the realization of large-area GEM foils.Comment: 4 pages, 10 figures, Presented at Vienna Conference on Instrumentation (Feb 15-20, 2010, Vienna, Austria). Submitted to the Proceeding

Gene electrotransfer of IL-2 and IL-12 plasmids effectively eradicated murine B16.F10 melanoma

Gene therapy has become an important approach for treating cancer, and electroporation represents a technology for introducing therapeutic genes into a cell. An example of cancer gene therapy relying on gene electrotransfer is the use of immunomodulatory cytokines, such as interleukin 2 (IL-2) and 12 (IL-12), which directly stimulate immune cells at the tumour site. The aim of our study was to determine the effects of gene electrotransfer with two plasmids encoding IL-2 and IL-12 in vitro and in vivo. Two different pulse protocols, known as EP1 (600 V/cm, 5 ms, 1 Hz, 8 pulses) and EP2 (1300 V/cm, 100 µs, 1 Hz, 8 pulses), were assessed in vitro for application in subsequent in vivo experiments. In the in vivo experiment, gene electrotransfer of pIL-2 and pIL-12 using the EP1 protocol was performed in B16.F10 murine melanoma. Combined treatment of tumours using pIL2 and pIL12 induced significant tumour growth delay and 71% complete tumour regression. Furthermore, in tumours coexpressing IL-2 and IL-12, increased accumulation of dendritic cells and M1 macrophages was obtained along with the activation of proinflammatory signals, resulting in CD4 + and CD8 + T-lymphocyte recruitment and immune memory development in the mice. In conclusion, we demonstrated high antitumour efficacy of combined IL-2 and IL-12 gene electrotransfer protocols in low-immunogenicity murine B16.F10 melanoma

Multicolor photometry of ten Seyfert 1 galaxies

We present BVI photometry of ten Seyfert 1 galaxies and narrow band H-alpha images for six of these objects as well. The results indicate that the luminosity sample distribution has an amplitude of almost 4 magnitudes with an average of M_B=-20.7. The observed morphologies are confined to early type galaxies. A barred structure is found in only 2 objects. Despite that early morphological types are dominant in this sample, integrated (B-V) colors are very blue. For instance, the SO galaxies show, on average, a (B-V)=0.78. This effect seems to be caused by the luminosity contribution of the active nucleus and/or the disk to the total luminosity of the galaxy. In the B band, the contribution of the active galactic nucleus to the total luminosity of the galaxy varies from 3% to almost 60% and the bulge to disk luminosity ratio (L_bulge/L_disk) ranges from 0.6 to 22. Signs of tidal interactions seems to be a common characteristic since they are observed in 6 of the objects and one of them seems to be located in a poor cluster not yet identified in the literature. H_alpha extended emission is rare, with only 1 galaxy showing clear evidence of it. Luminosity profile decomposition shows that the model Gauss + bulge + disk properly reproduces the surface brightness of the galaxies. However, in order to account for the luminosity profile, most of the disk galaxies needs the inner truncated exponential form with a central cutoff radius ranging from 3 to 10 kpc. This is interpreted in terms of reddened regions that are well identified in the B-V color maps. These regions present very similar colors among them, with (B-V)~1.2. This fact could be associated to the presence of dust confined in the inner regions of the galaxies.Comment: 14 pages, 25 figures. Accepted to Astronomy & Astrophysic

Measurement of {\eta} meson production in {\gamma}{\gamma} interactions and {\Gamma}({\eta}-->{\gamma}{\gamma}) with the KLOE detector

We present a measurement of {\eta} meson production in photon-photon interactions produced by electron-positron beams colliding with \sqrt{s}=1 GeV. The measurement is done with the KLOE detector at the \phi-factory DA{\Phi}NE with an integrated luminosity of 0.24 fb^{-1}. The e^+e^- --> e^+e^-{\eta} cross section is measured without detecting the outgoing electron and positron, selecting the decays {\eta}-->{\pi}^+{\pi}^-{\pi}^0 and {\eta}-->{\pi}^0{\pi}^0{\pi}^0. The most relevant background is due to e^+e^- --> {\eta}{\gamma} when the monochromatic photon escapes detection. The cross section for this process is measured as {\sigma}(e^+e^- -->{\eta}{\gamma}) = (856 \pm 8_{stat} \pm 16_{syst}) pb. The combined result for the e^+e^- -->e^+e^-{\eta} cross section is {\sigma}(e^+e^- -->e^+e^-{\eta}) = (32.72 \pm 1.27_{stat} \pm 0.70_{syst}) pb. From this we derive the partial width {\Gamma}({\eta}-->{\gamma}{\gamma}) = (520 \pm 20_{stat} \pm 13_{syst}) eV. This is in agreement with the world average and is the most precise measurement to date.Comment: Version accepted by JHE

A new limit on the CP violating decay KS -> 3pi0 with the KLOE experiment

We have carried out a new direct search for the CP violating decay KS -> 3pi0 with 1.7 fb^-1 of e+e- collisions collected by the KLOE detector at the phi-factory DAFNE. We have searched for this decay in a sample of about 5.9 x 10^8 KS KL events tagging the KS by means of the KL interaction in the calorimeter and requiring six prompt photons. With respect to our previous search, the analysis has been improved by increasing of a factor four the tagged sample and by a more effective background rejection of fake KS tags and spurious clusters. We find no candidates in data and simulated background samples, while we expect 0.12 standard model events. Normalizing to the number of KS -> 2pi0 events in the same sample, we set the upper limit on BR(KS -> 3pi0 < 2.6 x 10^-8 at 90% C.L., five times lower than the previous limit. We also set the upper limit on the eta_000 parameter, |eta_000 | < 0.0088 at 90% C.L., improving by a factor two the latest direct measurement.Comment: Accepted for publication in Physics Letters B (15 pages, 13 figures

Chemical abundances in the polar disk of NGC4650A: implications for cold accretion scenario

The aim of the present study is to test whether the cold accretion of gas through a "cosmic filament" Macci\`o et al. 2006 is a possible formation scenario for the polar disk galaxy NGC 4650A. If polar disks form from cold accretion of gas, the abundances of the HII regions may be similar to those of very late-type spiral galaxies, regardless of the presence of a bright central stellar spheroid, with total luminosity of few 10^9 Lsun. We use deep long slit spectra obtained with the FORS2 spectrograph at the VLT in the optical and near-infrared wavelength ranges for the brightest HII regions in the disk polar disk of NGC 4650A. The strongest emission lines ([OII] Hbeta, [OIII], Halpha) were used to derived oxygen abundances, metallicities and the global star formation rates for the disk. The deep spectra available allowed us to measure the Oxygen abundances (12 + log (O/H)) using the "Empirical method" based on intensities of the strongest emission lines, and the "Direct method", based on the determination of the electron temperature from the detection of weak auroral lines, as the [OIII] at 4363 Angstrom. The Oxygen abundance measured for the polar disk is then compared with those measured for different galaxy types of similar total luminosities, and then compared against the predictions of different polar ring formation scenarios. The average metallicity values for the polar disk in NGC 4650A is Z=0.2 Zsun, and it is lower that the values measured for ordinary spirals of similar luminosity. Moreover the gradient of the metallicity is flat along the polar disk major axis, which implies none or negligible metal enrichment from the stars in the older central spheroid. The low metallicity value in the polar disk NGC 4650A and the flat metallicity gradient are both consistent with a later infall of metal-poor gas, as expected in the cold accretion processes.Comment: 42 pages, 9 figures. Accepted for publication in Ap