Associations between lifestyle factors and an unhealthy diet

BACKGROUND: Unhealthy dietary patterns have been associated with other unhealthy lifestyle factors such as smoking and physical inactivity. Whether these associations are similar in high- and low-educated individuals is currently unknown. METHODS: We used information of the EPIC-NL cohort, a prospective cohort of 39 393 men and women, aged 20-70 years at recruitment. A lifestyle questionnaire and a validated food frequency questionnaire were administered at recruitment (1993-97). Low adherence to a Mediterranean-style diet was used to determine an unhealthy dietary pattern. Lifestyle-related factors included body mass index, waist circumference, smoking status, physical activity level, dietary supplement use and daily breakfast consumption. Multivariate logistic regression analyses were performed for the total population and by strata of educational level. RESULTS: In total 30% of the study population had an unhealthy dietary pattern: 39% in the lowest educated group and 20% in the highest educated group. Physical inactivity, a large waist circumference, no dietary supplement use and skipping breakfast were associated with an unhealthy dietary pattern in both low and high educated participants. Among low educated participants, current smokers had a greater odds of an unhealthy diet compared with never smokers: OR 1.42 (95% CI: 1.25; 1.61). This association was not observed in the high educated group. CONCLUSIONS: Most associations between lifestyle-related factors and unhealthy diet were consistent across educational levels, except for smoking. Only among low educated participants, current smokers reported an unhealthier dietary pattern in comparison to never smokers. These results can be used in the development of targeted health promotion strategies

Population-level consequences of complementary sex determination in a solitary parasitoid

BACKGROUND: Sex determination mechanisms are known to be evolutionarily labile but the factors driving transitions in sex determination mechanisms are poorly understood. All insects of the Hymenoptera are haplodiploid, with males normally developing from unfertilized haploid eggs. Under complementary sex determination (CSD), diploid males can be produced from fertilized eggs that are homozygous at the sex locus. Diploid males have near-zero fitness and thus represent a genetic load, which is especially severe under inbreeding. Here, we study mating structure and sex determination in the parasitoid Cotesia vestalis to investigate what may have driven the evolution of two complementary sex determination loci in this species.RESULTS: We genotyped Cotesia vestalis females collected from eight fields in four townships in Western Taiwan. 98 SNP markers were developed by aligning Illumina sequence reads of pooled DNA of eight different females against a de novo assembled genome of C. vestalis. This proved to be an efficient method for this non-model species and provides a resource for future use in related species. We found significant genetic differentiation within the sampled population but variation could not be attributed to sampling locations by AMOVA. Non-random mating was detected, with 8.1% of matings between siblings. Diploid males, detected by flow cytometry, were produced at a rate of 1.4% among diploids.CONCLUSIONS: We think that the low rate of diploid male production is best explained by a CSD system with two independent sex loci, supporting laboratory findings on the same species. Fitness costs of diploid males in C. vestalis are high because diploid males can mate with females and produce infertile triploid offspring. This severe fitness cost of diploid males combined with non-random mating may have resulted in evolution from single locus CSD to CSD with two independent loci.</p

Hansenula polymorpha Pex37 is a peroxisomal membrane protein required for organelle fission and segregation

Here, we describe a novel peroxin, Pex37, in the yeast Hansenula polymorpha. H. polymorpha Pex37 is a peroxisomal membrane protein, which belongs to a protein family that includes, among others, the Neurospora crassa Woronin body protein Wsc, the human peroxisomal membrane protein PXMP2, the Saccharomyces cerevisiae mitochondrial inner membrane protein Sym1, and its mammalian homologue MPV17. We show that deletion of H. polymorpha PEX37 does not appear to have a significant effect on peroxisome biogenesis or proliferation in cells grown at peroxisome‐inducing growth conditions (methanol). However, the absence of Pex37 results in a reduction in peroxisome numbers and a defect in peroxisome segregation in cells grown at peroxisome‐repressing conditions (glucose). Conversely, overproduction of Pex37 in glucose‐grown cells results in an increase in peroxisome numbers in conjunction with a decrease in their size. The increase in numbers in PEX37‐overexpressing cells depends on the dynamin‐related protein Dnm1. Together our data suggest that Pex37 is involved in peroxisome fission in glucose‐grown cells. Introduction of human PXMP2 in H. polymorpha pex37 cells partially restored the peroxisomal phenotype, indicating that PXMP2 represents a functional homologue of Pex37. H.polymorpha pex37 cells did not show aberrant growth on any of the tested carbon and nitrogen sources that are metabolized by peroxisomal enzymes, suggesting that Pex37 may not fulfill an essential function in transport of these substrates or compounds required for their metabolism across the peroxisomal membrane

6-thioguanine treatment in inflammatory bowel disease: A critical appraisal by a European 6-TG working party

Recently, the suggestion to use 6-thioguanine (6-TG) as an alternative thiopurine in patients with inflammatory bowel disease (IBD) has been discarded due to reports about possible (hepato) toxicity. During meetings arranged in Vienna and Prague in 2004, European experts applying 6-TG further on in IBD patients presented data on safety and efficacy of 6-TG. After thorough evaluation of its risk-benefit ratio, the group consented that 6-TG may still be considered as a rescue drug in stringently defined indications in IBD, albeit restricted to a clinical research setting. As a potential indication for administering 6-TG, we delineated the requirement for maintenance therapy as well as intolerance and/or resistance to aminosalicylates, azathioprine, 6-mercaptopurine, methotrexate and infliximab. Furthermore, indications are preferred in which surgery is thought to be inappropriate. The standard 6-TG dosage should not exceed 25 mg daily. Routine laboratory controls are mandatory in short intervals. Liver biopsies should be performed after 6-12 months, three years and then three-yearly accompanied by gastroduodenoscopy, to monitor for potential hepatotoxicity, including nodular regenerative hyperplasia (NRH) and veno-occlusive disease (VOD). Treatment with 6-TG must be discontinued in case of overt or histologically proven hepatotoxicity. Copyright (c) 2006 S. Karger AG, Basel

Modelling ice sheet evolution and atmospheric CO2 during the Late Pliocene

In order to investigate the relation between ice sheets and climate in a warmer-than-present world, recent research has focussed on the Late Pliocene, 3.6 to 2.58 million years ago. It is the most recent period in Earth's history when such a warm climate state existed for a significant duration of time. Marine Isotope Stage (MIS) M2 (∼3.3 Myr ago) is a strong positive excursion in benthic oxygen records in the middle of the otherwise warm and relatively stable Late Pliocene. However, the relative contributions to the benthic δ18O signal from deep ocean cooling and growing ice sheets are still uncertain. Here, we present results from simulations of the Late Pliocene with a hybrid ice-sheet–climate model, showing a reconstruction of ice sheet geometry, sea level and atmospheric CO2. Initial experiments simulating the last four glacial cycles indicate that this model yields results which are in good agreement with proxy records in terms of global mean sea level, benthic oxygen isotope abundance, ice-core-derived surface temperature and atmospheric CO2 concentration. For the Late Pliocene, our results show an atmospheric CO2 concentration during MIS M2 of 233–249 ppmv and a drop in global mean sea level of 10 to 25 m. Uncertainties are larger during the warmer periods leading up to and following MIS M2. CO2 concentrations during the warm intervals in the Pliocene, with sea-level high stands of 8–14 m above the present day, varied between 320 and 400 ppmv, lower than indicated by some proxy records but in line with earlier model reconstructions

Increased Adiposity, Dysregulated Glucose Metabolism and Systemic Inflammation in Galectin-3 KO Mice

PMCID: PMC3579848This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Evidence for Duality of Conifold from Fundamental String

We study the spectrum of BPS D5-D3-F1 states in type IIB theory, which are proposed to be dual to D4-D2-D0 states on the resolved conifold in type IIA theory. We evaluate the BPS partition functions for all values of the moduli parameter in the type IIB side, and find them completely agree with the results in the type IIA side which was obtained by using Kontsevich-Soibelman's wall-crossing formula. Our result is a quite strong evidence for string dualities on the conifold.Comment: 24 pages, 13 figures, v2: typos corrected, v3: explanations about wall-crossing improved and figures adde

Complex Odor from Plants under Attack: Herbivore's Enemies React to the Whole, Not Its Parts

Background: Insect herbivory induces plant odors that attract herbivores ’ natural enemies. Assuming this attraction emerges from individual compounds, genetic control over odor emission of crops may provide a rationale for manipulating the distribution of predators used for pest control. However, studies on odor perception in vertebrates and invertebrates suggest that olfactory information processing of mixtures results in odor percepts that are a synthetic whole and not a set of components that could function as recognizable individual attractants. Here, we ask if predators respond to herbivoreinduced attractants in odor mixtures or to odor mixture as a whole. Methodology/Principal Findings: We studied a system consisting of Lima bean, the herbivorous mite Tetranychus urticae and the predatory mite Phytoseiulus persimilis. We found that four herbivore-induced bean volatiles are not attractive in pure form while a fifth, methyl salicylate (MeSA), is. Several reduced mixtures deficient in one component compared to the full spider-mite induced blend were not attractive despite the presence of MeSA indicating that the predators cannot detect this component in these odor mixtures. A mixture of all five HIPV is most attractive, when offered together with the noninduced odor of Lima bean. Odors that elicit no response in their pure form were essential components of the attractive mixture. Conclusions/Significance: We conclude that the predatory mites perceive odors as a synthetic whole and that th

Hydrological impact of Middle Miocene Antarctic ice-free areas coupled to deep ocean temperatures

This is the author accepted manuscript. The final version is available from Nature Research via the DOI in this recordData availability The climate model output data are available for analysis and download at https://www.paleo.bristol.ac.uk/ummodel/scripts/papers/Bradshaw_et_al_2021.html. It is possible to reproduce the information in Figs. 2, 3, 5 and 6 via this interface as well as download the data itself and the ancillary information (palaeogeography and ice-sheet configuration).Oxygen isotopes from ocean sediments (δ O) used to reconstruct past continental ice volumes additionally record deep water temperatures (DWTs). Traditionally, these are assumed to be coupled (ice-volume changes cause DWT changes). However, δ O records during peak Middle Miocene warmth (~16–15 million years ago) document large rapid fluctuations (~1–1.5‰) difficult to explain as huge Antarctic ice sheet (AIS) volume changes. Here, using climate modelling and data comparisons, we show DWTs are coupled to AIS spatial extent, not volume, because Antarctic albedo changes modify the hydrological cycle, affecting Antarctic deep water production regions. We suggest the Middle Miocene AIS had retreated substantially from previous Oligocene maxima. The residual ice sheet varied spatially more rapidly on orbital timescales than previously thought, enabling large DWT swings (up to 4 °C). When Middle Miocene warmth terminated (~13 million years ago) and a continent-scale AIS had stabilized, further ice-volume changes were predominantly in height rather than extent, with little impact on DWT. Our findings imply a shift in ocean sensitivity to ice-sheet changes occurs when AIS retreat exposes previously ice-covered land; associated feedbacks could reduce the Earth system’s ability to maintain a large AIS. This demonstrates ice-sheet changes should be characterized not only by ice volume but also by spatial extent. 18 18Natural Environment Research CouncilNatural Environment Research CouncilSwedish Research Counci

Generalized Toda Theory from Six Dimensions and the Conifold

Recently, a physical derivation of the Alday-Gaiotto-Tachikawa correspondence has been put forward. A crucial role is played by the complex Chern-Simons theory arising in the 3d-3d correspondence, whose boundary modes lead to Toda theory on a Riemann surface. We explore several features of this derivation and subsequently argue that it can be extended to a generalization of the AGT correspondence. The latter involves codimension two defects in six dimensions that wrap the Riemann surface. We use a purely geometrical description of these defects and find that the generalized AGT setup can be modeled in a pole region using generalized conifolds. Furthermore, we argue that the ordinary conifold clarifies several features of the derivation of the original AGT correspondence.Comment: 27+2 pages, 3 figure