30 years of hay meadow succession without fertilization: how does it affect soil and avifauna groups?

In the present study we investigated the effects of hay meadow succession in the brook valley system of the Drentse A Nature Reserve, in the NE of the Netherlands. In particular, we compared the plant and soil fauna composition in five grasslands that differed in the stage of vegetation succession in two well-studied chronosequences, dry and wet respectively. The sampled meadows include a control site (still fertilized meadow), a 5, 15, 25 and 32 years stage of vegetation succession after the cessation of fertilizer application. At all sites, vegetation and earthworm composition was studied in replicate subplots of 4 m2 respectively 0.04 m² each. Moreover, the breeding birds have been monitored in the area over the last 28 years by mapping territories overlapping the meadows of the chronosequences. Concerning the plants we found that diversity was increasing with time of succession. In the wet meadow series the plant species richness increased from ca. 13 species per site (40 m2) to a maximum of ca. 49 species per site in the latest successional stage. In the drier parts the increase in species was less and reached an over all maximum of 27 species in the intermediate (15 years old) stage of the succession in 40 m2, but still increased to a mean of 15 species at the scale of 4 m2 plots in the oldest stage. The diversity and abundance of earthworms dropped significantly over time of succession. The species all belonged to 4 genera with Allolobophora being the most abundant. Soil pH dropped significantly during the succession even below 3.8 in the dry series. This largely explains the unfavourable conditions for the earthworms to survive in the oldest stages of the dry succession. The earthworm biomass dropped in nearly all sites below 25 g/m2 during the summer period, indicating unfavourable conditions for a suit of grassland breeding waders.The abundance of breeding birds in general was low due to the rather small area covered in this study. Anyhow we could find obvious changes in the breeding bird community. In particular waders such as Lapwing (Vanellus vanellus) and Black-tailed Godwit (Limosa limosa) disappeared completely from the area and were followed up by Curlew (Numenius arquata) and Snipe (Gallinago gallinago) being currently also rare in the area. Probably due to changes in grassland vegetation (increase of amongst others Cirsium palustre) and insect abundance the Stonechat (Saxicola torquata) has entered the area as a breeding bird. The same happens to a set of bird species typical for developing carr woodlands such as Lesser Spotted Woodpecker (Dendrocopos minor) and Golden Oriole (Oriolus oriolus).Die vorliegende Studie thematisiert am Beispiel der Fließgewässerniederung der Drentse A, einem großflächigen Schutzgebiet im Nordosten der Niederlande, die Folgen einer langjährigen Heuwiesennutzung ohne Düngung. Im Einzelnen wurde von uns die Zusammensetzung der Vegetation und Bodenfauna auf fünf Grünlandflächen untersucht, die sich in der Dauer der Ausmagerung unterschieden. Getrennt betrachtet wurden dabei die bachnahen, moorigen Niederungsbereichen und die angrenzenden sandigen Geestbereiche. Die ausgewählten Grünlandflächen waren zum Zeitpunkt der Aufnahmen seit 5, 15, 25, und 32 Jahren gemäht aber nicht mehr gedüngt worden. Eine weitere, nach wie vor konventionell bewirtschaftete Wiesenfläche (incl. Düngung) diente als Kontrolle. Auf allen Flächen wurde die Vegetation und Regenwurmfauna in 10 Plots mit einer Größe von jeweils 4 m² bzw. 0.04 m² untersucht. Darüber hinaus wurden über 28 Jahre hinweg die Brutvögel des Gebietes mittels Revierkartierung erfasst. Die Pflanzenartendiversität hat sich mit Dauer der Ausmagerung signifikant erhöht. Sie stieg in den bachnahen Bereichen von 13 Arten in der Kontrollfläche (40 m²) auf 49 Arten in der am längsten ausgemagerten Grünlandfläche an. In den trockenen Geestbereichen war der Anstieg deutlich schwächer. Bezogen auf die Gesamtfläche von 40 m² wurden die meisten Arten hier in der 15 Jahre lang ausgemagerten Grünlandfläche gefunden, während bei Betrachtung der 4 m² großen Aufnahmepunkte die höchste Artenzahl ebenfalls in der ältesten Untersuchungsfläche lag. Die Diversität und Abundanz der Regenwürmer nahm mit Dauer der Ausmagerung ab. Die festgestellten Arten gehörten zu 4 Gattungen, wobei die Gattung Allolobophora am individuenreichsten vertreten war. Mit Dauer der Ausmagerung sank besonders im trockenen bachfernen Geestbereich der Boden-pH-Wert auf unter 3,8 ab. Die damit einhergehenden pessimalen Lebensbedingungen erklären hinreichend die geringe Diversität und Dichte von Regenwürmern in diesen Bereichen. An fast allen Standorten sank die Biomasse der Regenwürmer zum Sommer hin auf Werte unter 25 g/m², so dass für viele Limikolen zu dieser Zeit pessimale Ernährungsbedingungen bestehen. Die Zahl der Brutvogelarten war aufgrund des recht kleinen Untersuchungsgebietes insgesamt gering. Dennoch konnten auffallende Veränderungen in der Brutvogelgemeinschaft beobachtet werden. Während Limikolen wie Kiebitz (Vanellus vanellus) und Uferschnepfe (Limosa limosa) vollständig aus dem Gebiet verschwanden, wanderten der Große Brachvogel und die Bekassine ein. Allerdings sind auch sie aktuell nur noch selten im Gebiet vertreten. Dafür hat sich inzwischen das Schwarzkehlchen (Saxicola torquata) als Brutvogel eingestellt – möglicherweise eine Folge der sich ändernden Grünlandvegetation (hier: Zunahme von Pflanzenarten, die als Ansitzwarten fungieren können wie etwa Cirsium palustre) in Kombination mit einem verbesserten Nahrungsangebot an Makroinvertebraten. Eingewandert sind zwischenzeitlich auch eine Reihe weiterer Vogelarten, wie Pirol (Oriolus oriolus) und Kleinspecht (Dendrocopos minor), die charakteristisch für sich entwickelnde Bruchwälder sind. Letztere haben sich, meist kleinflächig, auf ehemaligen Feuchtgrünlandstandorten entwickelt

The introduction of IMO, an integrated model for designing for open-­ended play

Designing for open-ended play poses specific new challenges to designers. Designing for closed games includes defining rules and goals to balanced the game properly. A design for open-ended play has no predefined rules and goals. The design needs to provide users with more freedom to continually change goals and rules of play, which distinguishes the field from designs of closed games. Gaining knowledge on the design process of creating this freedom is essential. For this purpose, an integrated model for open-ended play is proposed. This model is based on a combination of two existing models: Hunicke’s Mechanics Dynamics and Aesthetics (MDA) model and Grünvogel’s formal models for game design. Both of the above mentioned existing models are generalized to make them applicable for analyzing open-ended play. In the proposed combined model we distinguish between the perspectives of the design, and the perspective of play. It addresses how to handle changing rules and goals, instead of the assumptions that rules and goals do not change. Furthermore, the model was used to improve our understanding on progression and emergence, two key concepts that are commonly used in game design. The integrated model for open-ended play (IMO) was used in a preliminary case study with a digital play application, an interactive environment for open-ended play named the GlowSteps, to evaluate the model and to underline our insights on emergence and progression.Designing for open-ended play poses specific new challenges to designers. Designing for closed games includes defining rules and goals to balanced the game properly. A design for open-ended play has no predefined rules and goals. The design needs to provide users with more freedom to continually change goals and rules of play, which distinguishes the field from designs of closed games. Gaining knowledge on the design process of creating this freedom is essential. For this purpose, an integrated model for open-ended play is proposed. This model is based on a combination of two existing models: Hunicke’s Mechanics Dynamics and Aesthetics (MDA) model and Grünvogel’s formal models for game design. Both of the above mentioned existing models are generalized to make them applicable for analyzing open-ended play. In the proposed combined model we distinguish between the perspectives of the design, and the perspective of play. It addresses how to handle changing rules and goals, instead of the assumptions that rules and goals do not change. Furthermore, the model was used to improve our understanding on progression and emergence, two key concepts that are commonly used in game design. The integrated model for open-ended play (IMO) was used in a preliminary case study with a digital play application, an interactive environment for open-ended play named the GlowSteps, to evaluate the model and to underline our insights on emergence and progression

Focusing on the Fixed Point of 4D Simplicial Gravity

Our earlier renormalization group analysis of simplicial gravity is extended. A high statistics study of the volume and coupling constant dependence of the cumulants of the node distribution is carried out. It appears that the phase transition of the theory is of first order, contrary to what is generally believed.Comment: Latex, 20 pages, 6 postscript figures, published versio

Ant-infecting Ophiocordyceps genomes reveal a high diversity of potential behavioral manipulation genes and a possible major role for enterotoxins

Much can be gained from revealing the mechanisms fungal entomopathogens employ. Especially intriguing are fungal parasites that manipulate insect behavior because, presumably, they secrete a wealth of bioactive compounds. To gain more insight into their strategies, we compared the genomes of five ant-infecting Ophiocordyceps species from three species complexes. These species were collected across three continents, from five different ant species in which they induce different levels of manipulation. A considerable number of (small) secreted and pathogenicity-related proteins were only found in these ant-manipulating Ophiocordyceps species, and not in other ascomycetes. However, few of those proteins were conserved among them, suggesting that several different methods of behavior modification have evolved. This is further supported by a relatively fast evolution of previously reported candidate manipulation genes associated with biting behavior. Moreover, secondary metabolite clusters, activated during biting behavior, appeared conserved within a species complex, but not beyond. The independent co-evolution between these manipulating parasites and their respective hosts might thus have led to rather diverse strategies to alter behavior. Our data indicate that specialized, secreted enterotoxins may play a major role in one of these strategies

A socially interdependent choice framework for social influences in healthcare decision-making:a study protocol

OBJECTIVES: Current choice models in healthcare (and beyond) can provide suboptimal predictions of healthcare users' decisions. One reason for such inaccuracy is that standard microeconomic theory assumes that decisions of healthcare users are made in a social vacuum. Healthcare choices, however, can in fact be (entirely) socially determined. To achieve more accurate choice predictions within healthcare and therefore better policy decisions, the social influences that affect healthcare user decision-making need to be identified and explicitly integrated into choice models. The purpose of this study is to develop a socially interdependent choice framework of healthcare user decision-making.DESIGN: A mixed-methods approach will be used. A systematic literature review will be conducted that identifies the social influences on healthcare user decision-making. Based on the outcomes of a systematic literature review, an interview guide will be developed that assesses which, and how, social influences affect healthcare user decision-making in four different medical fields. This guide will be used during two exploratory focus groups to assess the engagement of participants and clarity of questions and probes. The refined interview guide will be used to conduct the semistructured interviews with healthcare professionals and users. These interviews will explore in detail which, and how, social influences affect healthcare user decision-making. Focus group and interview transcripts will be analysed iteratively using a constant comparative approach based on a mix of inductive and deductive coding. Based on the outcomes, a social influence independent choice framework for healthcare user decision-making will be drafted. Finally, the Delphi technique will be employed to achieve consensus about the final version of this choice framework.ETHICS AND DISSEMINATION: This study was approved by the Erasmus School of Health Policy and Management Research Ethics Review Committee (ESHPM, Rotterdam, The Netherlands; reference ETH2122-0666).</p

Timing and tempo of the great oxidation event

The first significant buildup in atmospheric oxygen, the Great Oxidation Event (GOE), began in the early Paleoproterozoic in association with global glaciations and continued until the end of the Lomagundi carbon isotope excursion ca. 2,060 Ma. The exact timing of and relationships among these events are debated because of poor age constraints and contradictory stratigraphic correlations. Here, we show that the first Paleoproterozoic global glaciation and the onset of the GOE occurred between ca. 2,460 and 2,426 Ma, ∼100 My earlier than previously estimated, based on an age of 2,426 ± 3 Ma for Ongeluk Formation magmatism from the Kaapvaal Craton of southern Africa. This age helps define a key paleomagnetic pole that positions the Kaapvaal Craton at equatorial latitudes of 11° ± 6° at this time. Furthermore, the rise of atmospheric oxygen was not monotonic, but was instead characterized by oscillations, which together with climatic instabilities may have continued over the next ∼200 My until ≤2,250–2,240 Ma. Ongeluk Formation volcanism at ca. 2,426 Ma was part of a large igneous province (LIP) and represents a waning stage in the emplacement of several temporally discrete LIPs across a large low-latitude continental landmass. These LIPs played critical, albeit complex, roles in the rise of oxygen and in both initiating and terminating global glaciations. This series of events invites comparison with the Neoproterozoic oxygen increase and Sturtian Snowball Earth glaciation, which accompanied emplacement of LIPs across supercontinent Rodinia, also positioned at low latitude

Measuring environmental health status in Oukasie, 1987

This article reports on some aspects of the physical environment and on envirorvnental health services at Oukasie. The data were collected over a weekend in August 1987 by an interview and by direct inspection of the environment using a standard schedule. We had a 100% response rate on the interviews. There was a mean of 2,2 ± 1,6 persons per room in the households, with brick houses being less crowded than non-brick houses. Private yards were generally kept very clean. Bucket latrines were unhygienic and used by an average of 4 families. No latrine was built to accepted standards. Garbage collection and emptying of the bucket latrines by the municipality seemed to be unsatisfactory. Our overall impression was that where responsibility for maintenance of hygiene is either undefined, such as the collection chambers of bucket latrines, or a responsibility of the public authorities, such as garbage collection and water drainage, there is an unsatisfactory state of hygiene. Where maintenance of cleanlness is clearly a private responsibility, such as the maintenance of the cleanliness of private yards, the general status of hygiene is good

Immunoproteasome-Deficiency Has No Effects on NK Cell Education, but Confers Lymphocytes into Targets for NK Cells in Infected Wild-Type Mice

Natural killer (NK) cells are part of the innate immune system and contribute to the eradication of virus infected cells and tumors. NK cells express inhibitory and activating receptors and their decision to kill a target cell is based on the balance of signals received through these receptors. MHC class I molecules are recognized by inhibitory receptors, and their presence during NK cell education influences the responsiveness of peripheral NK cells. We here demonstrate that mice with reduced MHC class I cell surface expression, due to deficiency of immunoproteasomes, have responsive NK cells in the periphery, indicating that the lower MHC class I levels do not alter NK cell education. Following adoptive transfer into wild-type (wt) recipients, immunoproteasome-deficient splenocytes are tolerated in naive but rejected in virus-infected recipients, in an NK cell dependent fashion. These results indicate that the relatively low MHC class I levels are sufficient to protect these cells from rejection by wt NK cells, but that this tolerance is broken in infection, inducing an NK cell-dependent rejection of immunoproteasome-deficient cells

Daily rhythms and enrichment patterns in the transcriptome of the behavior-manipulating parasite Ophiocordyceps kimflemingiae

Various parasite-host interactions that involve adaptive manipulation of host behavior display time-of-day synchronization of certain events. One example is the manipulated biting behavior observed in Carpenter ants infected with Ophiocordyceps unilateralis sensu lato. We hypothesized that biological clocks play an important role in this and other parasite-host interactions. In order to identify candidate molecular clock components, we used two general strategies: bioinformatics and transcriptional profiling. The bioinformatics approach was used to identify putative homologs of known clock genes. For transcriptional profiling, RNA-Seq was performed on 48 h time courses of Ophiocordyceps kimflemingiae (a recently named species of the O. unilateralis complex), whose genome has recently been sequenced. Fungal blastospores were entrained in liquid media under 24 h light-dark (LD) cycles and were harvested at 4 h intervals either under LD or continuous darkness. Of all O. kimflemingiae genes, 5.3% had rhythmic mRNAs under these conditions (JTK Cycle, <= 0.057 statistical cutoff). Our data further indicates that a significant number of transcription factors have a peaked activity during the light phase (day time). The expression levels of a significant number of secreted enzymes, proteases, toxins and small bioactive compounds peaked during the dark phase or subjective night. These findings support a model whereby this fungal parasite uses its biological clock for phase-specific activity. We further suggest that this may be a general mechanism involved in parasite-host interactions