Putting a spin on it: amyloid aggregation from oligomers to fibrils

This thesis focuses on amyloid proteins, a class of proteins that convert into amyloid fibrils. Such proteins are of high interest because they are related to many of the neurodegenerative diseases. In the brains of patients with neurodegenerative diseases, plaques of β-sheet amyloid aggregates are found, but the mechanism of their formation and their role vis-à-vis the disease are unknown. Aggregation is difficult to study because amyloids are intrinsically disordered proteins that lack an ordered structure in solution. Here we apply electron paramagnetic resonance (EPR) as a new technique to better understand the properties of amyloid oligomers and their formation.Biological and Soft Matter Physic

'ACCORD' e-Platform: Development and evaluation of an innovative multicultural training for school professionals

The study aims at describing the development, application and evaluation of ACCORD, an innovative e-platform offering a free-accessible tailored multicultural training for school professionals through the application of current ICT research (e-learning, mobility, internet, artificial intelligence). The eplatform provided school professionals with a dedicated Massive Online Open Course (MOOC) and a Serious Game (SG), embedded within it, useful to enhance and (self-)evaluate their intercultural competences and negotiation abilities. Technological, Psychological, and Pedagogical models and approaches underpinned the methodology driving the creation of the e-platform contents. Following a pilot test, the edited version of the e-platform (including the SG, learning materials, lessons, questionnaires) was widely diffused in different languages (English, German, Italian, Flemish, Spanish). Pre- and post-training questionnaires were used to assess the learning experience and the efficacy of the training. Findings provided evidence supporting the learning effectiveness of ACCORD training. The experience with the e-platform has been positively rated by the users concerning both the technological and educational aspects. Overall, the study provided an overview on the creation, implementation and evaluation of a novel multicultural training tool, which has been proven to effectively foster the enhancement of intercultural and interethnic competencies of professionals working in the European educational contexts

Experimental evidence of antiproton reflection by a solid surface

We report here experimental evidence of the reflection of a large fraction of a beam of low energy antiprotons by an aluminum wall. This derives from the analysis of a set of annihilations of antiprotons that come to rest in rarefied helium gas after hitting the end wall of the apparatus. A Monte Carlo simulation of the antiproton path in aluminum indicates that the observed reflection occurs primarily via a multiple Rutherford-style scattering on Al nuclei, at least in the energy range 1-10 keV where the phenomenon is most visible in the analyzed data. These results contradict the common belief according to which the interactions between matter and antimatter are dominated by the reciprocally destructive phenomenon of annihilation.Comment: 5 pages with 5 figure

Constraining the mass of the planet(s) sculpting a disk cavity. The intriguing case of 2MASS J16042165-2130284

The large cavities observed in the dust and gas distributions of transition disks may be explained by planet-disk interactions. At ~145 pc, 2MASS J16042165-2130284 (J1604) is a 5-12 Myr old transitional disk with different gap sizes in the mm- and $\mu$m-sized dust distributions (outer edges at ~79 and at ~63 au, respectively). Its $^{12}$CO emission shows a ~30 au cavity. This radial structure suggests that giant planets are sculpting this disk. We aim to constrain the masses and locations of plausible giant planets around J1604. We observed J1604 with the Spectro-Polarimetric High-contrast Exoplanet REsearch (SPHERE) at the Very Large Telescope (VLT), in IRDIFS\_EXT, pupil-stabilized mode, obtaining YJH- band images with the integral field spectrograph (IFS) and K1K2-band images with the Infra-Red Dual-beam Imager and Spectrograph (IRDIS). The dataset was processed exploiting the angular differential imaging (ADI) technique with high-contrast algorithms. Our observations reach a contrast of $\Delta K, YH$ ~12 mag from 0.15" to 0.80" (~22 to 115 au), but no planet candidate is detected. The disk is directly imaged in scattered light at all bands from Y to K, and it shows a red color. This indicates that the dust particles in the disk surface are mainly $\gtrsim0.3\,\mu$m-sized grains. We confirm the sharp dip/decrement in scattered light in agreement with polarized light observations. Comparing our images with a radiative transfer model we argue that the southern side of the disk is most likely the nearest. This work represents the deepest search yet for companions around J1604. We reach a mass sensitivity of $\gtrsim 2-3M_{Jup}$ from ~22 to ~115 au according to a hot start scenario. We propose that a brown dwarf orbiting inside of ~15 au and additional Jovian planets at larger radii could account for the observed properties of J1604 while explaining our lack of detection.Comment: 10 pages, 7 Figures. Accepted for publication in A&A . Abridged abstrac

Patterns of spatio-temporal change in Alpine ibex (Capra ibex ibex, L.) distribution in Gran Paradiso National Park

Ibex is the symbolic species of mountain ungulates and currently it is classified at Least Concern by IUCN. In the last few decades there was a drastic decrease in population abundance linked with a reduction of stable occupied territories. Causes are still not completely clear but drastic decline is in partly due to recent climate changes. The objectives of this study are to analyse the ibex distribution changes in Gran Paradiso National Park (GPNP) in 1985-2014 period, the type of relation between pattern of distribution and population trend and finally the evidence of most used ibex \u2018s territories in GPNP. To understand distribution patterns ibex census data are been analysed using metrics belonging to landscape ecology, an approach based on the notion that environmental patterns influence ecological processes. In particular 5 metrics are been used to assess the composition and spatial configuration of occupied areas, while distribution statistics provided a statistical summaries of obtained results. Results showed a reduction of ibex occupied territories from 4587.50 ha in 1985 to 2437.50 ha in 2014 and fragmentation of ibex occupied territories with a strong increase in number of occupied patches from 130 units to 229 units. We suggest that likely ibex distribution was influenced by different combined factors (landscape change, climate change, anthropic activities), which have to be still examined in depth. Landscape ecology approach may become an useful tool to understand the degree of fragmentation and connectivity of landscape defined on species distribution. The understanding of processes behind Alpine ungulates distribution have to consider the influence of landscape patterns on environmental processes to improve the conservation efforts at management level

An inner warp in the DoAr 44 T Tauri transition disk

Optical/IR images of transition disks (TDs) have revealed deep intensity decrements in the rings of HAeBes HD142527 and HD100453, that can be interpreted as shadowing from sharply tilted inner disks, such that the outer disks are directly exposed to stellar light. Here we report similar dips in SPHERE+IRDIS differential polarized imaging (DPI) of TTauri DoAr44. With a fairly axially symmetric ring in the submm radio continuum, DoAr44 is likely also a warped system. We constrain the warp geometry by comparing radiative transfer predictions with the DPI data in H band (Q_\phi(H)) and with a re-processing of archival 336GHz ALMA observations. The observed DPI shadows have coincident radio counterparts, but the intensity drops are much deeper in Q_\phi(H) (~88%), compared to the shallow drops at 336GHz (~24%). Radiative transfer predictions with an inner disk tilt of ~30+-5deg approximately account for the observations. ALMA long-baseline observations should allow the observation of the warped gas kinematics inside the cavity of DoAr44.Comment: 11 pages, 10 figures, 3 tables, accepted for publication in MNRA

The gravitational mass of Proxima Centauri measured with SPHERE from a microlensing event

Proxima Centauri, our closest stellar neighbour, is a low-mass M5 dwarf orbiting in a triple system. An Earth-mass planet with an 11 day period has been discovered around this star. The star's mass has been estimated only indirectly using a mass-luminosity relation, meaning that large uncertainties affect our knowledge of its properties. To refine the mass estimate, an independent method has been proposed: gravitational microlensing. By taking advantage of the close passage of Proxima Cen in front of two background stars, it is possible to measure the astrometric shift caused by the microlensing effect due to these close encounters and estimate the gravitational mass of the lens (Proxima Cen). Microlensing events occurred in 2014 and 2016 with impact parameters, the closest approach of Proxima Cen to the background star, of 1\farcs6 $\pm$ 0\farcs1 and 0\farcs5 $\pm$ 0\farcs1, respectively. Accurate measurements of the positions of the background stars during the last two years have been obtained with HST/WFC3, and with VLT/SPHERE from the ground. The SPHERE campaign started on March 2015, and continued for more than two years, covering 9 epochs. The parameters of Proxima Centauri's motion on the sky, along with the pixel scale, true North, and centering of the instrument detector were readjusted for each epoch using the background stars visible in the IRDIS field of view. The experiment has been successful and the astrometric shift caused by the microlensing effect has been measured for the second event in 2016. We used this measurement to derive a mass of 0.150$^{\textrm{+}0.062}_{-0.051}$ (an error of $\sim$ 40\%) \MSun for Proxima Centauri acting as a lens. This is the first and the only currently possible measurement of the gravitational mass of Proxima Centauri.Comment: 10 pages, 6 figures, accepted by MNRA

AF Lep b: the lowest mass planet detected coupling astrometric and direct imaging data

Aims. Using the direct imaging technique we searched for low mass companions around the star AF Lep that presents a significant proper motion anomaly (PMa) signal obtained from the comparison of Hipparcos and Gaia eDR3 catalogs. Methods. We observed AF Lep in two epochs with VLT/SPHERE using its subsystems IFS and IRDIS in the near-infrared (NIR) covering wavelengths ranging from the Y to the K spectral bands (between 0.95 and 2.3 {\mu}m). The data were then reduced using the high-contrast imaging techniques angular differential imaging (ADI) and spectral differential imaging (SDI) to be able to retrieve the signal from low mass companions of the star. Results. A faint companion was retrieved at a separation of ~0.335" from the star and with a position angle of ~70.5 deg in the first epoch and with a similar position in the second epoch. This corresponds to a projected separation of ~9 au. The extracted photometry allowed us to estimate for the companion a mass between 2 and 5 MJup. This mass is in good agreement with what is expected for the dynamic mass of the companion deduced using astrometric measures (5.2-5.5 MJup). This is the first companion with a mass well below the deuterium burning limit discovered coupling direct imaging with PMa measures. Orbit fitting done using the orvara tool allowed to further confirm the companion mass and to define its main orbital parameters.Comment: 10 pages, 8 Figures, accepted for publication on A&

In situ continuous wave electron paramagnetic resonance investigation of the amyloid aggregation of Parkinson's protein alpha-synuclein-the second spin label position

Self-aggregation of amyloid proteins is a crucial step in neurodegenerative disease. The protein alpha-synuclein (alpha S) is implicated in Parkinson's disease. In an extension of the demonstration of in situ observation of intermediates in alpha S-aggregation by continuous wave (cw) EPR at room temperature (Zurlo et al. PLoS One 16: e0245548, 2021) by spin-label EPR, here the spin label is attached to position 90 (R1 alpha S90), rather than at position 56. The aim is to determine, if the spin-label position affects the kinetics of aggregation and if local information on the intermediates is accessible. Probed by the MTSL ((1-Oxyl-2,2,5,5-tetramethylpyrroline-3-methyl) methanethiosulfonate) spin label at position 90, using diamagnetic dilution of 9:1 wild type alpha S to R1 alpha S90, similar aggregation kinetics are found. Rotation correlation times for the spin label in the oligomer cannot be determined with sufficient accuracy to obtain local information on the oligomer under the conditions used. At the present stage, higher resolution EPR approaches, such as high-field EPR are more promising.Biological and Soft Matter Physic