8 research outputs found
神経芽腫におけるPD-L1発現と腫瘍組織浸潤リンパ球の潜在的役割について
Purpose: The programmed death 1 (PD-1)/programmed death ligand 1 (PD-L1) pathway has garnered much attention for its roles in clinical oncology. The aim of this study was to examine the clinical impact of the PD-L1 expression and tumor-infiltrating lymphocytes (TILs) on neuroblastoma. Methods: We evaluated the PD-L1 expression and TIL status in 31 patients with neuroblastoma who underwent a biopsy or resection by an immunohistochemical analysis. Furthermore, we performed the serial analysis of the PD-L1 status before and after chemotherapy in 15 patients. Results: Among the 31 cases, 11 (35%) showed a positive PD-L1 expression. The survival analysis showed a trend toward an association between PD-L1 positivity and a decreased overall survival. PD-L1 positivity tended to be associated with higher levels of tumor markers. In the serial analysis of the PD-L1 status, positivity was noted in 8 of 15 patients before chemotherapy and 6 after chemotherapy. Notably, all four patients with a positive PD-L1 status both before and after chemotherapy had recurrence, and 3 of them died during the follow-up period. Conclusion: Our findings suggest that the PD-L1 tumor expression might be a good biomarker for the treatment of neuroblastoma patients, especially for advanced neuroblastoma.博士(医学)・乙第1458号・令和2年6月30日© Springer-Verlag GmbH Germany, part of Springer Nature 2020This is a post-peer-review, pre-copyedit version of an article published in Pediatric surgery international. The final authenticated version is available online at: http://doi.org/10.1007/s00383-019-04616-9
AXIS FOR ROTATION AT THE INTERVERTEBRAL JOINT IN JAPANESE MONKEYS
The position of axis for rotation at the intervertebral joint was investigated
using ten Japanese monkeys. The position of axis for rotation at the intervertebral joint
was shifted from dorsal to ventral direction on the superior and inferior views of the 1st
thoracic vertebra and was next shifted from ventral to dorsal direction on the superior
and inferior views of the 10th thoracic vertebra, with some exceptions. X-ray
examination demonstrated that in the Japanese monkeys, lordosis was seen in both the
cervical and lower lumbar(L5-L7) spine, whereas kyphosis was seen in the thoracic and
upper lumbar (L1-L4) spine. Therefore, the possibility that the position of axis for
rotation at the intervertebral joint was related to the curvature of the spinal column was
not supported by the present study
AXIS FOR ROTATION AT THE INTERVERTEBRAL JOINT IN JAPANESE MONKEYS
The position of axis for rotation at the intervertebral joint was investigated
using ten Japanese monkeys. The position of axis for rotation at the intervertebral joint
was shifted from dorsal to ventral direction on the superior and inferior views of the 1st
thoracic vertebra and was next shifted from ventral to dorsal direction on the superior
and inferior views of the 10th thoracic vertebra, with some exceptions. X-ray
examination demonstrated that in the Japanese monkeys, lordosis was seen in both the
cervical and lower lumbar(L5-L7) spine, whereas kyphosis was seen in the thoracic and
upper lumbar (L1-L4) spine. Therefore, the possibility that the position of axis for
rotation at the intervertebral joint was related to the curvature of the spinal column was
not supported by the present study
Application of Magnetic Nanoparticles to Gene Delivery
Nanoparticle technology is being incorporated into many areas of molecular science and biomedicine. Because nanoparticles are small enough to enter almost all areas of the body, including the circulatory system and cells, they have been and continue to be exploited for basic biomedical research as well as clinical diagnostic and therapeutic applications. For example, nanoparticles hold great promise for enabling gene therapy to reach its full potential by facilitating targeted delivery of DNA into tissues and cells. Substantial progress has been made in binding DNA to nanoparticles and controlling the behavior of these complexes. In this article, we review research on binding DNAs to nanoparticles as well as our latest study on non-viral gene delivery using polyethylenimine-coated magnetic nanoparticles