Clonal variation in cell surface display of an H-2 protein lacking a cytoplasmic tail

Truncated variants of the gene encoding H-2Ld, an integral membrane protein encoded by the major histocompatibility complex, were constructed by in vitro mutagenesis to elucidate the function of charged amino acids found on the cytoplasmic side of the transmembrane (TM) region. Analysis of cloned L cells transfected with these genes shows that the seven amino acids following the TM segment, four of which are basic, enhance the cell surface expression of H-2Ld protein but are not required for it. However, some clones do not express a tailless H-2Ld protein on the cell surface but express it intracellularly where it has a long half-life. Turnover measurements on cell surface H-2Ld proteins suggest that the basic residues following the TM segment are not a "stop transfer" sequence (Blobel, G., 1980, Proc. Natl. Acad. Sci. USA., 77:1496-1500) which anchors the H-2Ld protein in the membrane. Pulse-chase and endoglycosidase H sensitivity studies show that H-2Ld proteins lacking some or all of the basic residues and H-2Ld proteins which have a full-length cytoplasmic tail are processed with different kinetics. These results suggest an involvement of the membrane-proximal region of the cytoplasmic tail in the intracellular transport of H-2Ld. We further suggest that the L cell clones which do and do not express a tailless H-2Ld protein on the cell surface differ in the ability to transport a tailless integral membrane protein to the cell surface

Policy and Literature Review on the Effect Millennials Have on Vehicle Miles Traveled, Greenhouse Gas Emissions, and the Built Environment

Vehicle travel has reduced substantially across all demographics in the 2000’s, but millennials or young adults born from 1985-2000 stand out as the group that has reduced vehicle travel the most. This reduction of travel among millennials is known as the millennial effect. This policy and literature review discusses insights from recent policy reports and literature regarding the millennial effect and identifies the prominent themes and gaps in our knowledge. The first section reviews existing research on the millennial effect on vehicle miles traveled (VMT). The second section discusses the influence of the built environment on the travel and activities of the millennial generation. The third section highlights scenarios describing the millennials effect’s potential magnitude and identifies topics for consideration in future scenario planning efforts. The final section discusses the uncertainty that exists regarding the future behavior of millennials and their influence on VMT and greenhouse gas emissions

Continental Guadalajara Services Mexico S.A de C.V, Santa Anita

ITESO, A.C

El lenguaje tecnológico en las prácticas de enseñanza en la formación del profesorado. Algunas consideraciones iniciales

El presente trabajo tiene como propósito exponer una serie de consideraciones en torno a las prácticas de enseñanza que involucran al lenguaje tecnológico. Estas consideraciones surgen del proceso llevado a cabo para la construcción e implementación de propuestas que incluyen el uso del lenguaje tecnológico en las prácticas de formación del profesorado de Educación Especial. Los aspectos a mencionar emergen del análisis acerca de las diferentes actividades desarrolladas en el marco de la línea de investigación PRÁCTICAS DE ENSEÑANZA. Lenguaje Tecnológico y comprensión en espacios de formación en la carrera del Profesorado de Educación Especial del PROICO 4-1316 “Prácticas de Enseñanza para la comprensión. Su impacto en la formación de los estudiantes de las carreras de Profesorados de Educación Inicial y Especial” de la Facultad de Ciencias Humanas, de la Universidad Nacional de San Luis. Nuestro eje central de estudio se basa en cómo el espacio de investigación y la relación interdisciplinar de sus miembros promueve la realización de prácticas de investigación y de formación que favorecen la construcción del lenguaje tecnológico y su integración significativa en las prácticas de enseñanza dentro del aula universitaria

Estrategias de seguridad clínica para reducir la ocurrencia de infecciones asociadas a la atención en salud durante una cesárea

Antecedentes: La cesárea es uno de los procedimientos más frecuentes realizados en el área de la obstetricia, se conoce que aumenta el riesgo de morbimortalidad materna; su proceso ha cambiado tras la implementación de estrategias y utilización de insumos que disminuyen el riesgo de complicaciones asociadas a infecciones. Objetivo: Describir las intervenciones desde la seguridad clínica que reducen el riesgo de complicaciones infecciosas en la realización de una cesárea. Metodología: Se realizó búsqueda de la literatura entre agosto y octubre del 2020, utilizando las palabras clave descritas y términos alternativos en las bases de datos: Pubmed, Google académico y clinicalkey, se identificaron los estudios relevantes, para soportar con evidencia la redacción del artículo. Resultados: La literatura soporta, que las pacientes que son llevadas a cesárea tienen un riesgo mayor de complicaciones infecciosas comparado con el parto vaginal de 5 a 10 veces más. Estas complicaciones, pueden estar asociadas a factores propios del paciente y al procedimiento mismo. Algunas intervenciones son probadas para disminuir las complicaciones quirúrgicas, entre otras, la selección correctamente del pacientes con indicación precisa, la implementación de la lista de chequeo de cirugía segura, la administración de profilaxis antimicrobiana previo a procedimiento, la adecuada antisepsia de la piel, la adecuada preparación vaginal e inserción de sonda vesical en el menor tiempo posible, que va dirigida a evitar lesión de órgano pero que puede aumentar el riesgo de infección. Conclusión: Adherirse a las intervenciones soportadas por la evidencia resulta una estrategia importante para la reducción de complicaciones infecciosas asociadas a la cesárea

Estimation of the rigid transformation between two cameras from the Fundamental Matrix VS from Homographies.

The 3D reconstruction is an important step for the analytical calculation of the Jacobian of the image in a process of visual control of robots. In a two-camera stereo system that reconstruction depends on the knowledge of the rigid transformation between the two cameras and is represented by the rotation and translation between them. These two parameters are the result of a calibration of the stereo pair, but can also be retrieved from the epipolar geometry of the system, or from a homography obtained by features belonging to a flat object in the scene. In this paper, we make an assessment of the latter two alternatives, taking as reference an Euclidean reconstruction eliminating image distortion. We analyze three cases: the distortion inherent in the camera is corrected, without corrected distortion, and when Gaussian noise is added to the detection of features

Variability of the prevalence of depression in function of sociodemographic and environmental factors: ecological model

Major depression etiopathogenesis is related to a wide variety of genetics, demographic and psychosocial factors, as well as to environmental factors. The objective of this study is to analyze sociodemographic and environmental variables that are related to the prevalence of depression through correlation analysis and to develop a regression model that explains the behavior of this disease from an ecological perspective. This is an ecological, retrospective, cross-sectional study. The target population was 1,148,430 individuals over the age of 16 who were registered in Aragon (Spain) during 2010, with electronic medical records in the community’s primary health care centers. The spatial unit was the Basic Health Area (BHA). The dependent variable was the diagnosis of Depression and the ecological independent variables were: Demographic variables (gender and age), population distribution, typology of the entity, population structure by sex and age, by nationality, by education, by work, by salary, by marital status, structure of the household by number of members, and state of the buildings. The results show moderate and positive correlations with higher rates of depression in areas having a higher femininity index, higher population density, areas with a higher unemployment rate and higher average salary. The results of the linear regression show that aging +75 and rural entities act as protective factors for depression, while urban areas and deficient buildings act as risk factors. In conclusion, the ecological methodology may be a useful tool which, together with the statistical epidemiological analysis, can help in the political decision making process

Correlation between Zn vacancies and photoluminescence emission in ZnO films.

Photoluminescence and positron annihilation spectroscopy have been used to characterize and identify vacancy-type defects produced in ZnO films grown on sapphire by metal-organic chemical-vapor deposition. The photoluminescence of the samples in the near band edge region has been studied, paying particular attention to the emission at 370.5 nm (3.346 eV). This emission has been correlated to the concentration of Zn vacancies in the films, which has been determined by positron annihilation [email protected] [email protected]