Genetic variability and character association for bulb yield and yield related traits in garlic in Ethiopia

Garlic ( Allium sativum ) has for centuries been valued by humans for food, culinary and medicinal purposes world over. The objective of this study was to investigate genetic variability among garlic accessions for yield, yield related and phenology traits in Ethiopia. A field study was conducted at the DebreZeit Agricultural Research Center during 2012, using 49 garlic accessions from the highlands of North Shewa, East and West Arsi, Arsi, Bale and Sidama zones, which are among the major garlic producing areas in Ethiopia. The experiment was arranged in a 7x7 simple Lattice design, with two replications. Accession were highly significant (P < 0.01) for days to maturity, leaf number per plant, neck diameter, yield per plant, biological yield per plant, dry weight above ground, bulb dry weight, dry weight underground, clove number per bulb, and clove weight per bulb. Heritability estimates ranged from 82.48% for clove number, to 6.46% harvest index. High heritability, combined with high genetic advance (as per cent of mean) observed for mean clove number, yield per plant, biological yield per plant and clove weight per plant showed that these characters were controlled by additive gene effects. Thus phenotypic selection for these characters would likely be effective in variety selection and development. Bulb yield per plant had positive and highly significant genotypic and phenotypic correlations, with all characters, except plant height and harvest index. Path analysis at phenotypic level revealed that biological yield and bulb dry weight contributed major positive direct effects to bulb yield per plant. These traits showed positive and highly significant genotypic correlations with bulb yield except harvest indexL\u2019ail ( Allium sativum ) est appr\ue9ci\ue9 depuis des si\ue8cles par les humains \ue0 des fins alimentaires, culinaires et m\ue9dicinales dans le monde entier. L\u2019objectif de cette \ue9tude \ue9tait d\u2019\ue9tudier la variabilit\ue9 g\ue9n\ue9tique entre les accessions d\u2019ail pour le rendement et le rendement li\ue9s traits ph\ue9nologiques en \uc9thiopie. Une \ue9tude de terrain a \ue9t\ue9 men\ue9e au centre de recherche agricole DebreZeit en 2012, en utilisant 49 accessions d\u2019ail des zones montagneuses du Nord de Shewa, de l\u2019Est et de l\u2019Ouest d\u2019Arsi, Arsi, Bale et Sidama, qui sont parmi les principales zones de production d\u2019ail en \uc9thiopie. L\u2019exp\ue9rience a \ue9t\ue9 organis\ue9e dans une conception de treillis simple 7x7, avec deux r\ue9plications. L\u2019accession \ue9tait tr\ue8s significative (P <0,01) pour les jours jusqu\u2019\ue0 la maturit\ue9, le nombre de feuilles par plante, le diam\ue8tre du col, le rendement par plante, le rendement biologique par plante, le poids sec au-dessus du sol, le poids sec du bulbe, le poids sec sous terre, le nombre de clou de girofle par bulbe, et le poids de clou de girofle par bulbe. Les estimations d\u2019h\ue9ritabilit\ue9 variaient de 82,48% pour le nombre de clous de girofle \ue0 6,46% d\u2019indice de r\ue9colte. Une h\ue9ritabilit\ue9 \ue9lev\ue9e, combin\ue9e \ue0 une avanc\ue9e g\ue9n\ue9tique \ue9lev\ue9e (en pourcentage de la moyenne) observ\ue9e pour le nombre moyen de clous de girofle, le rendement par plante, le rendement biologique par plante et le poids de clou de girofle par plante ont montr\ue9 que ces caract\ue8res \ue9taient contr\uf4l\ue9s par des effets g\ue9n\ue9tiques additifs. Ainsi, la s\ue9lection ph\ue9notypique pour ces caract\ue8res serait probablement efficace dans la s\ue9lection et le d\ue9veloppement des vari\ue9t\ue9s. Le rendement en bulbes par plante avait des corr\ue9lations g\ue9notypiques et ph\ue9notypiques positives et tr\ue8s significatives, avec tous les caract\ue8res, \ue0 l\u2019exception de la hauteur de la plante et de l\u2019indice de r\ue9colte. L\u2019analyse des chemins au niveau ph\ue9notypique a r\ue9v\ue9l\ue9 que le rendement biologique et le poids sec du bulbe contribuaient \ue0 des effets directs positifs majeurs sur le rendement en bulbe par plante. Ces caract\ue8res ont montr\ue9 des corr\ue9lations g\ue9notypiques positives et tr\ue8s significatives avec le rendement des bulbes, \ue0 l\u2019exception de l\u2019indice de r\ue9colt

Lung function reduction and chronic respiratory symptoms among workers in the cement industry: a follow up study

<p>Abstract</p> <p>Background</p> <p>There are only a few follow-up studies of respiratory function among cement workers. The main aims of this study were to measure total dust exposure, to examine chronic respiratory symptoms and changes in lung function among cement factory workers and controls that were followed for one year.</p> <p>Methods</p> <p>The study was conducted in two cement factories in Ethiopia. Totally, 262 personal measurements of total dust among 105 randomly selected workers were performed. Samples of total dust were collected on 37-mm cellulose acetate filters placed in closed faced Millipore-cassettes. Totally 127 workers; 56 cleaners, 44 cement production workers and 27 controls were randomly selected from two factories and examined for lung function and interviewed for chronic respiratory symptoms in 2009. Of these, 91 workers; 38 cement cleaners (mean age 32 years), 33 cement production workers (36 years) and 20 controls (38 years) were examined with the same measurements in 2010.</p> <p>Results</p> <p>Total geometric mean dust exposure among cleaners was 432 mg/m³. The fraction of samples exceeding the Threshold Limit Value (TLV) of 10 mg/m³for the cleaners varied from 84-97% in the four departments. The levels were considerably lower among the production workers (GM = 8.2 mg/m³), but still 48% exceeded 10 mg/m³.</p> <p>The prevalence of all the chronic respiratory symptoms among both cleaners and production workers was significantly higher than among the controls.</p> <p>Forced Expiratory Volume in one second (FEV₁) and FEV₁/Forced Vital Capacity (FEV₁/FVC) were significantly reduced from 2009 to 2010 among the cleaners (p < 0.002 and p < 0.004, respectively) and production workers (p < 0.05 and p < 0.02, respectively), but not among the controls.</p> <p>Conclusions</p> <p>The high prevalence of chronic respiratory symptoms and reduction in lung function is probably associated with high cement dust exposure. Preventive measures are needed to reduce the dust exposure.</p

Incidence and predictors of puerperal sepsis among postpartum women at Debre Markos comprehensive specialized hospital, northwest Ethiopia: A prospective cohort study

Background: Puerperal sepsis is one of the leading causes of maternal mortality, particularly in low and middle-income countries where most maternal deaths occur. Women with puerperal sepsis are prone to long-term disabilities, such as chronic pelvic pain, blocked fallopian tubes, and secondary infertility. Besides this, puerperal sepsis has received less attention. For this reason, this study aimed to determine the incidence of puerperal sepsis and its predictors among postpartum women at Debre Markos Comprehensive Specialized Hospital. Methods: A prospective cohort study was conducted among 330 postpartum women from September 2020 to 2021. A pre-tested interviewer-administered questionnaire with a data extraction checklist was used to collect the data. Data were entered into Epi data 4.2 and analyzed using STATA 14.0. The incidence rate of puerperal sepsis was calculated, and a Kaplan-Meier survival curve was used to estimate the survival probability of developing puerperal sepsis. The cox-proportional hazards regression model was fitted to identify predictors of puerperal sepsis. Results: The study participants were followed for a total of 1685.3 person-week observations. The incidence rate of puerperal sepsis was 14.24 per 1,000 person-weeks. However, the overall incidence of puerperal sepsis was 7.27%. Not attending formal education [AHR: 3.55, 95% CI: (1.09–11.58)], a cesarean delivery [AHR: 4.50; 95% CI: (1.79–11.30)], premature rupture of the membranes [AHR: 3.25; 95% CI: (1.08–9.79)], complicated pregnancy [AHR: 4.80; 95% CI: (1.85–12.43)], being referred [AHR: 2.90; 95% CI: (1.10–7.65)], and not having birth preparedness and complication readiness plan [AHR: 2.95; 95% CI: (1.08–10.50)] were statistically significant predictors of puerperal sepsis. Conclusion: The incidence of puerperal sepsis was 7.27%. Not attending formal education, cesarean delivery, premature rupture of membranes, complicated pregnancy, referral status, and absence of birth preparedness and complication readiness plan were predictors associated with the incidence of puerperal sepsis

Effect of a self-care educational intervention to improve self-care adherence among patients with chronic heart failure: a clustered randomized controlled trial in Northwest Ethiopia

Background: As the burden of cardiovascular disease increases in sub-Saharan Africa, there is a growing need for low-cost interventions to mitigate its impact. Providing self-care health education to patients with chronic heart failure (CHF) is recommended as an intervention to prevent complications, improve quality of life, and reduce financial burdens on fragile health systems. However, little is known about health education’s effectiveness at improving CHF self-management adherence in sub-Saharan Africa. Therefore the present study aimed to assess the effectiveness of an educational intervention to improve self-care adherence among patients with CHF at Debre Markos and Felege Hiwot Referral Hospitals in Northwest Ethiopia. Methods: To address this gap, we adapted a health education intervention based on social cognitive theory comprising of intensive four-day training and, one-day follow-up sessions offered every four months. Patients also received illustrated educational leaflets. We then conducted a clustered randomized control trial of the intervention with 186 randomly-selected patients at Debre Markos and Felege Hiwot referral hospitals. We collected self-reported data on self-care behavior before each educational session. We analyzed these data using a generalized estimating equations model to identify health education's effect on a validated 8-item self-care adherence scale. Results: Self-care adherence scores were balanced at baseline. After the intervention, patients in the intervention group (n = 88) had higher adherence scores than those in the control group (n = 98). This difference was statistically significant (β = 4.15, p < 0.05) and increased with each round of education. Other factors significantly associated with adherence scores were being single (β = − 0.25, p < 0.05), taking aspirin (β = 0.76, p < 0.05), and having a history of hospitalization (β = 0.91, p < 0.05). Conclusions: We find that self-care education significantly improved self-care adherence scores among CHF patients. This suggests that policymakers should consider incorporating self-care education into CHF management

Prevalence and assemblage of Giardia duodenalis in a case-control study of children under 5 years from Jimma, Southwest Ethiopia

Giardia duodenalis is a common pathogenic intestinal protozoan parasite with high prevalence in developing countries, especially among children. The distribution of giardia assemblages among humans and their clinical relevance remains controversial. This study aimed to determine the prevalence and assemblage of Giardia among children under 5 years of age in Jimma, Southwest Ethiopia. Employing a case-control design, 606 children presenting with diarrhea at Jimma university medical center and Serbo Health Center were enrolled from December 2016 to July 2018 along with 617 matched controls without diarrhea. Giardia was detected and typed using real-time PCR. Univariate and multivariate regression analysis was performed. The total prevalence of Giardia was 41% (501/1223) and did not differ significantly between cases and controls (40% vs 42%). Prevalence increased by age, with the highest prevalence seen in children aged ≥ 25 months. Children without diarrhea with a history of diarrhea during the last month were more likely to be Giardia positive compared to children with no history diarrhea (OR 1.8 and 95%CI; 1.1–2.9). Regardless of current diarrhea symptoms, assemblage B predominated with 89%, followed by assemblage A (8%) and mixed infection assemblage A and B (3%). We report a high prevalence of Giardia by PCR detection in Jimma, Ethiopia, with assemblage B being predominant. There was a similar distribution of Giardia assemblages between children with and without diarrhea. Increasing age was a risk factor for Giardia infection. Community-based prevention and control strategies need to be employed to decrease the risk of giardia infection

Cement dust exposure and acute lung function: A cross shift study

Background: Few studies have been carried out on acute effects of cement dust exposure. This study is conducted to investigate the associations between current "total" dust exposure and acute respiratory symptoms and respiratory function among cement factory workers. Methods: A combined cross-sectional and cross-shift study was conducted in Dire Dawa cement factory in Ethiopia. 40 exposed production workers from the crusher and packing sections and 20 controls from the guards were included. Personal "total" dust was measured in the workers' breathing zone and peak expiratory flow (PEF) was measured for all selected workers before and after the shift. When the day shift ended, the acute respiratory symptoms experienced were scored and recorded on a five-point Likert scale using a modified respiratory symptom score questionnaire. Results: The highest geometric mean dust exposure was found in the crusher section (38.6 mg/m3) followed by the packing section (18.5 mg/m3) and the guards (0.4 mg/m3). The highest prevalence of respiratory symptoms for the high exposed workers was stuffy nose (85%) followed by shortness of breath (47%) and "sneezing" (45%). PEF decreased significantly across the shift in the high exposed group. Multiple linear regression showed a significant negative association between the percentage cross-shift change in PEF and total dust exposure. The number of years of work in high-exposure sections and current smoking were also associated with cross-shift decrease in PEF. Conclusions: Total cement dust exposure was related to acute respiratory symptoms and acute ventilatory effects. Implementing measures to control dust and providing adequate personal respiratory protective equipment for the production workers are highly recommended

Enhanced multifunctionality of CuO nanoparticles synthesized using aqueous leaf extract of Vernonia amygdalina plant

We report the synthesis of medicinal plant, Vernonia amygdalina Del. mediated green copper oxide nanoparticles (VeA-CuO NPs). The presence of two absorbance maxima, λmax 1 and λmax 2 at 436 nm and 452 nm, respectively confirms a mixture of biomolecules surface amalgamated CuO NPs with different morphological features. The FT-IR spectra of the plant leaf extract and VeA-CuO confirmed the efficient role of biomolecules as capping and stabilising agents. The XRD patterns of NPs approved high crystallinity of CuO. The purity of the NPs was corroborated by SEM-EDAX analysis. The average particle size of the NPs was found to be 19.68 nm. In addition, the combined TEM, HRTEM and SAED analysis substantiated the presence of CuO with a d-spacing value of 0.2854 nm, which conformed to CuO (1 1 1). The antibacterial assay revealed that VeA-CuO NPs were synergistic in their influence versus bacterial strains, S. aureus, E. coli, P. aeruginosa, and E. aerogenes. The uppermost zone of inhibition of 15 mm was observed for E. aerogenes. The bioactive compounds capped around the CuO NPs served the effective role in disrupting the cell wall of bacterial strains. The degradation efficiencies for Indigo carmine (IC) and Malachite green (MG) dyes by NPs were found to be 95% and 91%, respectively. The lowest degradation half-life was recorded to be 16.55 min for MG dye. In addition, the better electrode stability revealed by CV and EIS studies, confirms the multi-functional nature of VeA-CuO NPs, these CuO NPs exhibited multifunctional applications