Characterization of rat bone marrow lymphoid cells. I. A study of the distribution parameters of sedimentation velocity, volume and electrophoretic mobility

Various cell populations in rat bone marrow were characterized by means of a two dimensional separation using velocity sedimentation and free flow electrophoresis and by electrical sizing of the separated cells. Up to 4.5 mm/hr five different populations with discrete distributions in volume (coefficient of variation 10% to 13%) and sedimentation velocity (coefficient of variation 6% to 10%) were observed. Three of the small sized populations represented lymphocytes and small normoblasts and two of the larger sized populations represented myeloid cells. Almost all of these cells were in the G0/G1 cycle phase. In the faster sedimenting fractions which contained immature myeloid, erythroid and undefined blast cells and two S phase populations, discrete volume distributions were not evaluated. The cell populations with homogeneous volume (particularly the small lymphocytes) showed high density variations which condiserably impair the separation resolution. The cells sedimenting slower than 3.5 mm/hr were further separated by means of free flow electrophoresis into three peaks differing in electrophoretic mobility (EPM). The peaks of low and high EPM contained two populations and the peak of medium EPM contained three populations all characterized by normal volume distributions of uniform coefficient of variation between 11% and 14%. The small cells in the peaks of high and medium EPM were normolblasts and the other cells were lymphocytes. The biological significance of these results is discussed

M.Z. Topographia Electorat. Brandenburgici et Ducatus Pomeraniae &c. das ist Beschreibung der Vornembsten und bekantisten Stätte und Plätz in dem hochlöblichsten Churfürstenthum und March Brandenburg; und dem Hertzogtum Pom[m]eren, ec.

http://tartu.ester.ee/record=b1811158~S1*es

Bibliothekarische Ausbildung im Spannungsfeld von Informationsmanagement und Digitalisierung

Die Dominanz von digitalem Content und Digitalisierungsmaßnahmen verändern die Berufswelt von Beschäftigten in Bibliotheken. Die dafür benötigten digitalen Kompetenzen erfordern ständige Adaptierungen in den Ausbildungsprogrammen. Allerdings unterliegen diese Ausbildungsprogramme mehreren Spannungsfeldern, um aktuelle Ausbildungsinhalte anbieten zu können. Unterschiedliche Spannungsfelder, unter anderem Spezialisierung vs. breite Wissensbasis, der Anteil an Informationstechnologien, eine Schwerpunktsetzung im Informationsmanagement oder der Bedarf an einem offenen, erweiterten Kompetenzspektrum werden diskutiert.The dominance of digital content and digitization are changing the professional world of library employees. The new and changed digital competencies require constant adaptations in the training programs. However, these programs are subject to several conflicts in order to be able to offer up-to-date teaching content. Different areas of conflict are discussed, including specialization vs. broad knowledge base, the amount of information technologies, a focus on information management, or the need for an open, broadened spectrum of competencies.Peer Reviewe

Hispaniae et Lusitaniae itinerarium : nova et accurata descriptione, iconibusq novis et elegantibus loca earundem præcipua illustrans

Sign.: )(12, 2*8, 3*1, [ ]1, A-P12, Q2, R10, S12, T5Prefacio y redacción de Martin Zeiller, cuyo nombre aparece al final del prólogoPort. grab. calcGrab. de San Sebastián hacia 1600Digitalización. Vitoria-Gasteiz : Fundación Sancho el Sabio, 2008Digitalización. Vitoria-Gasteiz : Archivos y Bibliotecas, Mayo 1997Pergamin

Phospholipase D protects ECV304 cells against TNFα-induced apoptosis

AbstractTumor necrosis factor α (TNFα), a pleiotropic cytokine, activates both apoptotic and pro-survival signals depending on the cell model. Using ECV304 cells, which can be made TNFα-sensitive by cycloheximide (CHX) co-treatment, we evaluated the potential roles of ceramide and phospholipase D (PLD) in TNFα-induced apoptosis. TNFα/CHX induced a robust increase in ceramide levels after 16h of treatment when cell death was maximal. PLD activity was increased at early time point (1h) whereas both PLD activity and PLD1 protein were strongly decreased after 24h. TNFα/CHX-induced cell death was significantly lowered by exogenous bacterial PLD and phoshatidic acid, and in cells overexpressing PLD1. Conversely, cells depleted in PLD proteins by small interference RNA (siRNA) treatment exhibited higher susceptibility to apoptosis. These results show that PLD exerts a protective role against TNFα-induced cell death

Enfants et adolescents criminels: Aspects psychopathologiques

Os autores estudaram os processos de 106 menores de 18 anos, aos quais foi imputada a práAtica de um crime, escolhidos de entre os casos decididos pelos tribunais da região parisiense. No termo de uma investigação que consistiu em quatro entrevistas semi-directivas separadas por um intervalo de três meses, às quais se associou a aplicação de testes de personalidade (Rorschach e T.A.T.), os autores tentaram pôr em relevo os factores psicopatologicos comuns a estes jovens delinquentes e sugerem orientações com vista ao diagnostico precoce, à prevenção e à intervenção terapêutica.Les auteurs ont étudié les dossiers de 106 jeunes poursuivis pour crime devant des ïlibunaux de la région parisienne à partir des documents judiciaires d’affaires criminels de mineurs (de moins de 18 ans). Au terme d’une recherche consistant en quatre entretiens semi-directifs séparés par un intervalle de trois mois et comportant des tests de personnalité (Rorschach et T.A.T.), ils ont tenté de dégager les facteurs psychopathologiques communs à ces jeunes crimindsAmongst the criminal records of minors (under 18)prosecuted in courts of the Parisian area, the authors have studied the files of 106 young people. Their research, consisting of four semi-directive interviews separated by a three month period, includes personnality tests (Rorschach and T.A.T.) and aims at a definition of the psychopathological factors common to these young criminals and at guidelines for (early) diagnosis, prevention and therapeutical help

Das Meisterwerk als Autorität. Zur Wirkmacht kultureller Figurationen

Meisterwerke sind allgegenwärtig: Obwohl der Begriff wissenschaftlich überholt erscheint, begegnet er uns doch tagtäglich. Museen, Verlage und Konzerthäuser werben mit diesem Etikett für Werke als ausgezeichnete Vorbilder, denen unsere ungeteilte Aufmerksamkeit gebührt. Das wirft die Frage auf, welche Faktoren den Status von Artefakten als Meisterwerke begründen und tradieren. Auch Autorität ist allgegenwärtig: Als auszeichnende Eigenschaft strukturiert sie unsere Gesellschaft und ermöglicht, steuert oder verhindert soziales Handeln. Verbindet man den Meisterwerkbegriff mit dem der Autorität, eröffnen sich neue Perspektiven, um die Funktion und Dynamik von ästhetischen Vorbildern zu verstehen. Der vorliegende Band untersucht so das Meisterwerk als Autorität in unterschiedlichen wissenschaftlichen Disziplinen und verdeutlicht im Wechsel der Fragestellungen die Erkenntnismöglichkeiten dieses Konzeptes