Modular Design via Multiple Anion Chemistry of the High Mobility van der Waals Semiconductor Bi₄O₄SeCl₂

Making new van der Waals materials with electronic or magnetic functionality is a chemical design challenge for the development of two-dimensional nanoelectronic and energy conversion devices. We present the synthesis and properties of the van der Waals material Bi4O4SeCl2, which is a 1:1 superlattice of the structural units present in the van der Waals insulator BiOCl and the three-dimensionally connected semiconductor Bi2O2Se. The presence of three anions gives the new structure both the bridging selenide anion sites that connect pairs of Bi2O2 layers in Bi2O2Se and the terminal chloride sites that produce the van der Waals gap in BiOCl. This retains the electronic properties of Bi2O2Se while reducing the dimensionality of the bonding network connecting the Bi2O2Se units to allow exfoliation of Bi4O4SeCl2 to 1.4 nm height. The superlattice structure is stabilized by the configurational entropy of anion disorder across the terminal and bridging sites. The reduction in connective dimensionality with retention of electronic functionality stems from the expanded anion compositional diversity

A contribution of star-forming clumps and accreting satellites to the mass assembly of z ∼ 2 galaxies

We investigate the contribution of clumps and satellites to the galaxy mass assembly. We analysed spatially resolved HubbleSpace Telescope observations (imaging and slitless spectroscopy) of 53 star-forming galaxies at z ∼ 1–3. We created continuum and emission line maps and pinpointed residual ‘blobs’ detected after subtracting the galaxy disc. Those were separated into compact (unresolved) and extended (resolved) components. Extended components have sizes ∼2 kpc and comparable stellar mass and age as the galaxy discs, whereas the compact components are 1.5 dex less massive and 0.4 dex younger than the discs. Furthermore, the extended blobs are typically found at larger distances from the galaxy barycentre than the compact ones. Prompted by these observations and by the comparison with simulations, we suggest that compact blobs are in situ formed clumps, whereas the extended ones are accreting satellites. Clumps and satellites enclose, respectively, ∼20 per cent and ≲80 per cent of the galaxy stellar mass, ∼30 per cent and ∼20 per cent of its star formation rate. Considering the compact blobs, we statistically estimated that massive clumps (M⋆ ≳ 109 M⊙) have lifetimes of ∼650 Myr, and the less massive ones (108 < M⋆ < 109 M⊙) of ∼145 Myr. This supports simulations predicting long-lived clumps (lifetime ≳ 100 Myr). Finally, ≲30 per cent (13 per cent) of our sample galaxies are undergoing single (multiple) merger(s), they have a projected separation ≲10 kpc, and the typical mass ratio of our satellites is 1:5 (but ranges between 1:10 and 1:1), in agreement with literature results for close pair galaxies

Stellar feedback in a clumpy galaxy at z ∼ 3.4

Giant star-forming regions (clumps) are widespread features of galaxies at z ≈ 1−4. Theory predicts that they can play a crucial role in galaxy evolution, if they survive to stellar feedback for >50 Myr. Numerical simulations show that clumps’ survival depends on the stellar feedback recipes that are adopted. Up to date, observational constraints on both clumps’ outflows strength and gas removal time-scale are still uncertain. In this context, we study a line-emitting galaxy at redshift z ≃ 3.4 lensed by the foreground galaxy cluster Abell 2895. Four compact clumps with sizes ≲280 pc and representative of the low-mass end of clumps’ mass distribution (stellar masses ≲2 × 108 M⊙) dominate the galaxy morphology. The clumps are likely forming stars in a starbursting mode and have a young stellar population (∼10 Myr). The properties of the Lyman-α (Lyα) emission and nebular far-ultraviolet absorption lines indicate the presence of ejected material with global outflowing velocities of ∼200–300 km s−1. Assuming that the detected outflows are the consequence of star formation feedback, we infer an average mass loading factor (η) for the clumps of ∼1.8–2.4 consistent with results obtained from hydrodynamical simulations of clumpy galaxies that assume relatively strong stellar feedback. Assuming no gas inflows (semiclosed box model), the estimates of η suggest that the time-scale over which the outflows expel the molecular gas reservoir (≃7 × 108 M⊙) of the four detected low-mass clumps is ≲50 Myr

A pivotal role for starch in the reconfiguration of 14C-partitioning and allocation in Arabidopsis thaliana under short-term abiotic stress.

Plant carbon status is optimized for normal growth but is affected by abiotic stress. Here, we used 14C-labeling to provide the first holistic picture of carbon use changes during short-term osmotic, salinity, and cold stress in Arabidopsis thaliana. This could inform on the early mechanisms plants use to survive adverse environment, which is important for efficient agricultural production. We found that carbon allocation from source to sinks, and partitioning into major metabolite pools in the source leaf, sink leaves and roots showed both conserved and divergent responses to the stresses examined. Carbohydrates changed under all abiotic stresses applied; plants re-partitioned 14C to maintain sugar levels under stress, primarily by reducing 14C into the storage compounds in the source leaf, and decreasing 14C into the pools used for growth processes in the roots. Salinity and cold increased 14C-flux into protein, but as the stress progressed, protein degradation increased to produce amino acids, presumably for osmoprotection. Our work also emphasized that stress regulated the carbon channeled into starch, and its metabolic turnover. These stress-induced changes in starch metabolism and sugar export in the source were partly accompanied by transcriptional alteration in the T6P/SnRK1 regulatory pathway that are normally activated by carbon starvation

HER1-Targeted 86Y-Panitumumab Possesses Superior Targeting Characteristics than 86Y-Cetuximab for PET Imaging of Human Malignant Mesothelioma Tumors Xenografts

Malignant mesothelioma (MM), a rare form of cancer is often associated with previous exposure to fibrous minerals, such as asbestos. Asbestos exposure increases HER1-activity and expression in pre-clinical models. Additionally, HER1 over-expression is observed in the majority of MM cases. In this study, the utility of HER1-targeted chimeric IgG(1), cetuximab, and a human IgG(2), panitumumab, radiolabeled with (86)Y, were evaluated for PET imaging to detect MM non-invasively in vivo, and to select an antibody candidate for radioimmunotherapy (RIT).Radioimmunoconjugates (RICs) of cetuximab and panitumumab were prepared by conjugation with CHX-A''-DTPA followed by radiolabeling with (86)Y. The HER1 expression of NCI-H226, NCI-H2052, NCI-H2452 and MSTO-211H human mesothelioma cells was characterized by flow cytometry. In vivo biodistribution, pharmacokinetic analysis, and PET imaging were performed in tumor bearing athymic mice.In vivo studies demonstrated high HER1 tumor uptake of both RICs. Significant reduction in tumor uptake was observed in mice co-injected with excess mAb (0.1 mg), demonstrating that uptake in the tumor was receptor specific. Significant differences were observed in the in vivo characteristics of the RICs. The blood clearance T(½)α of (86)Y-cetuximab (0.9-1.1 h) was faster than (86)Y-panitumumab (2.6-3.1 h). Also, the tumor area under the curve (AUC) to liver AUC ratios of (86)Y-panitumumab were 1.5 to 2.5 times greater than (86)Y-cetuximab as observed by the differences in PET tumor to background ratios, which could be critical when imaging orthotopic tumors and concerns regarding radiation doses to normal organs such as the liver.This study demonstrates the more favorable HER1-targeting characteristics of (86)Y-panitumumab than (86)Y-cetuximab for non-invasive assessment of the HER1 status of MM by PET imaging. Due to lower liver uptake, panitumumab based immunoconjugates may fare better in therapy than corresponding cetuximab based immunoconjugates

Systems microscopy approaches to understand cancer cell migration and metastasis

Cell migration is essential in a number of processes, including wound healing, angiogenesis and cancer metastasis. Especially, invasion of cancer cells in the surrounding tissue is a crucial step that requires increased cell motility. Cell migration is a well-orchestrated process that involves the continuous formation and disassembly of matrix adhesions. Those structural anchor points interact with the extra-cellular matrix and also participate in adhesion-dependent signalling. Although these processes are essential for cancer metastasis, little is known about the molecular mechanisms that regulate adhesion dynamics during tumour cell migration. In this review, we provide an overview of recent advanced imaging strategies together with quantitative image analysis that can be implemented to understand the dynamics of matrix adhesions and its molecular components in relation to tumour cell migration. This dynamic cell imaging together with multiparametric image analysis will help in understanding the molecular mechanisms that define cancer cell migration