231 research outputs found
Photocatalytic activity of nitrogen-doped and undoped titanium dioxide sputtered thin films
In the present work titanium dioxide (TiO2) thin films were grown by d.c. reactive magnetron sputtering process, systematically varying the Ar/O2 ratio in the gas mixture, in order to study the influence of the oxygen partial pressure on the crystallographic structure and photocatalytic activity of the TiO2 thin films. After the sputtering process the TiO2 coatings were nitrided in a microwave (f= 2.45 GHz) Electron Cyclotron Resonance (ECR) plasma discharge in pure nitrogen, to compare the photocatalytic activity of undoped and nitrogen-doped TiO2 thin films. The crystal structure of the TiO2 grown samples was studied by x-ray diffraction (XRD) and the presence of the anatase phase in these films were corroborated by Raman spectroscopy. On the other hand, X-ray photoelectron spectroscopy (XPS) measurements carried out in the nitrogen-doped TiO2 samples, showed that the nitrogen was incorporated to the films with an average concentration of 18 at% of N. The UV-Vis optical spectroscopy allowed calculating the band gap. A narrowing of the optical band gap from 3.2 eV for the undoped films to 2.5 eV for the N-doped films was observed. Photocatalytic activity tests were done using a methylene blue (MB) dye solution. The irradiation of the films in the MB dye solution was carried out with an emission lamp in the UV and in the visible range for undoped and N-doped TiO2 films, respectively. The results showed that the N-doped TiO2 films had a higher photocalytic activity in the visible range, reaching a greater MB degradation in comparison with undoped samples, which were subjected to a higher energy radiation.Fil: Franco Arias, Lina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Valle; ColombiaFil: Zambrano, G.. Universidad del Valle; ColombiaFil: Gómez, M. E.. Universidad del Valle; ColombiaFil: Camps, E.. Instituto Nacional de Investigaciones Nucleares; MéxicoFil: Escobar Alarcón, L.. Instituto Nacional de Investigaciones Nucleares; Méxic
Comparison Between the Material Improvement of the Megarok and San José Quarries, Applying the AASTHO Standards
Comparative analyzes were carried out between the materials of the Magarok and San José quarries, taking into account AASTHO standards with the objective of being used as a base / sub-base in the pavement structure. The asphalt emulsion material was stabilized to increase the strength and give the material sub-base and / or base characteristics, with a suitable mixing design, obtaining results with suitable characteristics to be used in the pavement structure composed of a 65 % Asphalt AC 20; 34.30% water and 0.70% ASFIER 121 emulsions, with good resistance to the stability of the materials
Development of a genetic tool for functional screening of anti-malarial bioactive extracts in metagenomic libraries
BACKGROUND: The chemical treatment of Plasmodium falciparum for
human infections is losing efficacy each year due to the rise of
resistance. One possible strategy to find novel anti-malarial
drugs is to access the largest reservoir of genomic biodiversity
source on earth present in metagenomes of environmental
microbial communities. METHODS: A bioluminescent P. falciparum
parasite was used to quickly detect shifts in viability of
microcultures grown in 96-well plates. A synthetic gene encoding
the Dermaseptin 4 peptide was designed and cloned under tight
transcriptional control in a large metagenomic insert context
(30 kb) to serve as proof-of-principle for the screening
platform. RESULTS: Decrease in parasite viability consistently
correlated with bioluminescence emitted from parasite
microcultures, after their exposure to bacterial extracts
containing a plasmid or fosmid engineered to encode the
Dermaseptin 4 anti-malarial peptide. CONCLUSIONS: Here, a new
technical platform to access the anti-malarial potential in
microbial environmental metagenomes has been developed
Remoción del colorante azul marino directo sobre borra de café modificada
The presence of dyes in water bodies inhibits the penetration of light, affecting the flora and fauna of these ecosystems, which is why, greater efforts are made to eliminate them before being poured. This study allowed the removal of the direct navy-blue dye (DNB), using activated carbon prepared from coffee beans and H3PO4. The experimental methodology began with the preparation of three types of activated carbon by varying the concentration of H3PO4 (20, 40 and 60% m/v). Texture properties were evaluated by adsorption-desorption isotherms with N2 to 77 K, the identification and quantification of organic functional groups, mainly acids, with FTIR and the Boehm method, respectively. Batch adsorption experiments were performed by varying the initial dye concentration (5, 10, 50, 75, 100 and 200 mg/dm3) to 25 °C and, the adsorption kinetics was determined. Both coffee beans and activated carbons have an acidic nature with surface area development between 519 and 771 m2/g. With respect to the batch study, a monolayer and multilayer growth was observed on a heterogeneous surface. Activated carbon prepared with 20% of H3PO4 recorded the highest removal capacity with a value of 25.8 mg/g. The kinetic model of pseudo second order was the one that best fit to the experimental data (R2 > 0.98). It can be concluded that the coffee bean treated with H3PO4 is an efficient adsorbent to remove DNB from aqueous solutions.La presencia de colorantes en los cuerpos de agua inhibe la penetración de la luz, afectando la flora y la fauna de estos ecosistemas, razón por la cual se hacen cada vez esfuerzos mayores para eliminarlos antes de ser vertidos. Este estudio permitió remover el colorante azul marino directo (AMD), empleando carbón activado preparado a partir de la borra de café y H3PO4. La metodología experimental inició con la preparación de tres tipos de carbón activado, variando la concentración de H3PO4 (20, 40 y 60% m/v). Las propiedades de textura se evaluaron mediante isotermas de adsorción-desorción con N2 a 77 K; la identificación y cuantificación de grupos funcionales orgánicos, en especial ácidos, con FTIR, y el método de Boehm, respectivamente. Se realizaron experimentos de adsorción por lote, variando la concentración inicial del colorante (5, 10, 50, 75, 100 y 200 mg/dm3) a 25 °C y se determinó la cinética de adsorción. Tanto la borra de café como los carbones activados tienen naturaleza ácida con desarrollo de áreas superficiales entre 519 y 771 m2/g. Con respecto al estudio por lote, se observó un crecimiento en monocapa y multicapa sobre una superficie heterogénea. El carbón activado preparado con 20% de H3PO4 registró la mayor capacidad de remoción, con un valor de 25.8 mg/g. El modelo cinético de pseudo segundo orden fue el que mejor se ajustó a los datos experimentales (R2 > 0.98). Se puede concluir que la borra de café tratada con H3PO4 es un adsorbente eficiente para eliminar AMD de soluciones acuosas
Development of a genetic tool for functional screening of anti-malarial bioactive extracts in metagenomic libraries
Ajuts: Departamento Administrativo de Ciencias, Tecnología e Innovación (Colciencias), República de Colombia; Convocatoria 489 - 2009, Código 657048925406, Contrato de financiación RC. 427 - 2009 Colciencias - CorpoGen; Programa de Asistencias Graduadas de Universidad de los Andes, Bogotá, Colombia; i Programa Jóvenes Investigadores de ColcienciasBACKGROUND: The chemical treatment of Plasmodium falciparum for human infections is losing efficacy each year due to the rise of resistance. One possible strategy to find novel anti-malarial drugs is to access the largest reservoir of genomic biodiversity source on earth present in metagenomes of environmental microbial communities. METHODS: A bioluminescent P. falciparum parasite was used to quickly detect shifts in viability of microcultures grown in 96-well plates. A synthetic gene encoding the Dermaseptin 4 peptide was designed and cloned under tight transcriptional control in a large metagenomic insert context (30 kb) to serve as proof-of-principle for the screening platform. RESULTS: Decrease in parasite viability consistently correlated with bioluminescence emitted from parasite microcultures, after their exposure to bacterial extracts containing a plasmid or fosmid engineered to encode the Dermaseptin 4 anti-malarial peptide. Here, a new technical platform to access the anti-malarial potential in microbial environmental metagenomes has been develope
NGF inhibits apoptosis in memory B lymphocytes via inactivation of p38 MAPK, prevention of Bcl-2 phosphorylation and cytochrome c release.
Nerve growth factor inhibits apoptosis in memory B lymphocytes via inactivation of p38 MAPK, prevention of Bcl-2 phosphorylation, and cytochrome c release
Multidisciplinary design of suitable assistive technologies for motor disabilities in Colombia
raumatic or pathological brain lesions often result in motor disabilities that have strong personal and social effects. Assistive technologies can support this population, potentially improving their autonomy and promoting their participation in society. However, most existing research does not explicitly consider socio-cultural aspects, which differ between developing and developed countries. In this paper, we describe a multidisciplinary research line on technology-based assistive solutions for motor disabilities involving institutions in Colombia and Switzerland. The key aspect of our approach is the involvement of engineers, therapists, designers and end-users from early stages of the design process. This allowed us to characterize the local population with motor disabilities, highlighting a large incidence of violence- related injuries, reduced accessibility to assistive technologies and a perception of social exclusion. In the quest for context- suited solutions, we have developed a mechanical wheelchair and a sensorized facility for motor rehabilitation. The prototypes of these devices will be tested in the upcoming months. Importantly, we established a training program that uniquely covers both clinical and technical aspects of motor rehabilitation; providing experts from different domains with a common knowledge that facilitates the multidisciplinary work, enabling us to initiate experiments on clinical research; thus strengthening the links between academic, clinical and rehabilitation institutions
Amerindian Helicobacter pylori Strains Go Extinct, as European Strains Expand Their Host Range
We studied the diversity of bacteria and host in the H. pylori-human model. The human indigenous bacterium H. pylori diverged along with humans, into African, European, Asian and Amerindian groups. Of these, Amerindians have the least genetic diversity. Since niche diversity widens the sets of resources for colonizing species, we predicted that the Amerindian H. pylori strains would be the least diverse. We analyzed the multilocus sequence (7 housekeeping genes) of 131 strains: 19 cultured from Africans, 36 from Spanish, 11 from Koreans, 43 from Amerindians and 22 from South American Mestizos. We found that all strains that had been cultured from Africans were African strains (hpAfrica1), all from Spanish were European (hpEurope) and all from Koreans were hspEAsia but that Amerindians and Mestizos carried mixed strains: hspAmerind and hpEurope strains had been cultured from Amerindians and hpEurope and hpAfrica1 were cultured from Mestizos. The least genetically diverse H. pylori strains were hspAmerind. Strains hpEurope were the most diverse and showed remarkable multilocus sequence mosaicism (indicating recombination). The lower genetic structure in hpEurope strains is consistent with colonization of a diversity of hosts. If diversity is important for the success of H. pylori, then the low diversity of Amerindian strains might be linked to their apparent tendency to disappear. This suggests that Amerindian strains may lack the needed diversity to survive the diversity brought by non-Amerindian hosts
Vacunación del adulto en atención primaria: durante y después de pandemia
Immunization is one of the most important advances in medicine, achieving protection against numerous microorganisms that have put the health of the population at risk for centuries and is currently one of the main health prevention strategies; Recently, with the appearance of COVID-19, a before and after has been marked, since vaccination has regained visibility and importance after the pandemic. However, despite the available evidence, there is limited dissemination and promotion of vaccination in the adult, a situation that generates low coverage in this population. The current global context sets an urgent call to strengthen the implementation of vaccination plans and strategies not only in the pediatric population, but also in the adult population, so the objective of this narrative review is to synthesize the current recommendations for vaccination in the population. from Colombia and Latin America with a focus on primary care in the adult population. For this, a bibliographic search was carried out in the PubMed, Science direct, Redalyc, Scielo and academic Google databases; Including studies related to vaccination in adults in Colombia and Latin America, which allow to propose systematized vaccination strategies in primary care.La inmunización es uno de los avances más importantes de la medicina, logrando la protección contra numerosos microorganismos que han puesto en riesgo la salud de la población durante siglos y siendo en la actualidad una de las principales estrategias de prevención en salud; recientemente con la aparición del COVID-19 se ha marcado un antes y un después, pues a partir de la pandemia la vacunación recobró visibilidad e importancia, sin embargo, a pesar de la evidencia disponible, existe una limitada divulgación y promoción de la vacunación en el adulto, situación que genera una baja cobertura en esta población. El contexto global actual configura un llamado urgente a fortalecer la implementación de planes y estrategias de vacunación no sólo en la población pediátrica, sino también en la población adulta, por lo que el objetivo de esta revisión narrativa es sintetizar las recomendaciones actuales de vacunación en la población adulta de Colombia y América Latina con un enfoque en atención primaria en la población adulta. Para esto, se llevó a cabo una búsqueda bibliográfica en las bases de datos PubMed, Science direct, Redalyc, Scielo y Google académico; Incluyendo estudios relacionados con la vacunación en el adulto en Colombia y América Latina, los cuales permiten plantear estrategias sistematizadas de vacunación en atención primaria
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