« Bonne fortune, galanterie et jeu sont fort souvent capricieux ». Stabilité et crise des rôles sexués dans le jeu à l'époque moderne

Depuis le début de l'époque moderne, il n'est guère tant question de chance que dans le contexte du discours sur le jeu. Etant l'un des moyens de divertissement et de sociabilité le plus couramment utilisé, le jeu devient un thème sérieux dans de nombreux écrits moralistes, théologiques, philosophiques, juridiques, littéraires et techniques traitant de la juste façon d'utiliser temps et argent. En témoignent les classifications en jeux de hasard, d'adresse et en jeux dits mixtes (comme par ex..

Playthings in Early Modernity: Party Games, Word Games, Mind Games

Why do we play games—with and upon each other as well as ourselves? When are winners also losers, and vice-versa? How and to what end do we stretch the spaces of play? What happens when players go ‘out of bounds,’ or when games go ‘too far’? Moreover, what happens when we push the parameters of inquiry: when we play with traditional narratives of ludic culture, when we re-write the rules? An innovative volume of fifteen interdisciplinary essays at the nexus of material culture, performance studies, and game theory, Playthings in Early Modernity emphasizes the rules of the game(s) as well as the breaking of those rules. Thus, the titular ‘plaything’ is understood as both an object and a person, and play, in the early modern world, is treated not merely as a pastime, a leisurely pursuit, but as a pivotal part of daily life, a strategic psychosocial endeavor

Calcio fiorentino revisited: a bibliographical puzzle finally solved

Tese de Mestrado em Arte Neo-Realista Portuguesa apresentada à Faculdade de Letras da Universidade de CoimbraDesenvolve-se este estudo sobre a Fase Negra (1942-45) do pintor Manuel Filipe (1908-1992) no contexto da sua polémica recepção em Portugal e da repressão que sobre ele se abateu. Esclarecendo aspectos fundamentais da sua vida (como desde quando começou a expor, ou se efetivamente escreveu e veiculou algum pensamento teórico estético sobre a arte e os artistas coevos a Manuel Filipe), que permaneciam esquecidos ou ignorados, tentou-se entender a sua série de carvões à luz da emergente corrente neo-realista da qual, na vertente visual e gráfica, foi um dos pioneiros entre nós, fortemente influenciado pelo expressionismo alemão e a nova objectividade, mas também pelos muralistas mexicanos, entretanto muito divulgados entre nós. A série dramática estrutura-se tematicamente à volta dos excluídos e dos marginalizados, da exploração operária e da exaltação do papel da Mulher, constituindo um amplo painel, formalmente não homogéneo, pois utiliza várias linguagens plásticas, de uma galeria de monstros e de um bestiário inumano representativo da atitude crítica do autor e testemunho da difícil situação da produção artística e cultural no auge da ditadura do Estado Novo

Novel RP-HPLC based assay for selective and sensitive endotoxin quantification

The paper presents a novel instrumental analytical endotoxin quantification assay. It uses common analytical laboratory equipment (HPLC-FLD) and allows quantifying endotoxins (ETs) in different matrices from about 109 EU / mL down to about 40 EU / mL (RSE based). Test results are obtained in concentration units (e.g. ng ET / mL), which can then be converted to commonly used endotoxin units (EU / mL) in case of known pyrogenic activity. During endotoxin hydrolysis, the endotoxin specific rare sugar acid KDO is obtained quantitatively. After that, KDO is stoichiometrically reacted with DMB, which results in a highly fluorescent derivative. The mixture is separated using RP-HPLC followed by KDO-DMB quantification with a fluorescence detector. Based on the KDO content, the endotoxin content in the sample is calculated. The developed assay is economic and has a small error. Its applicability was demonstrated in applied research. ETs were quantified in purified bacterial biopolymers, which were produced by Gram-negative bacteria. Results were compared to LAL results obtained for the same samples. A high correlation was found between the results of both methods. Further, the new assay was utilized with high success during the development of novel endotoxin specific depth filters, which allow efficient, economic and sustainable ET removal during DSP. Those examples demonstrate that the new assay has the potential to complement the animal-based biological LAL pyrogenic quantification tests, which are accepted today by the major health authorities worldwide for the release of commercial pharmaceutical products