IMMUNOMODULATORS IN PULMOLOGIGAL PRACTICE

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Composición lipídica de diferentes partes del fruto del aguaymanto (Physalis peruviana L.) y valorización de residuos de semillas y cáscaras

The consumption of Cape gooseberry (Physalis peruviana L.) fruit (CG), fresh or processed, is gaining popularity worldwide, due to its nutritional and medicinal benefits. This study was based on the analysis of the lipid fraction of different parts of CG fruit and on further valorization of the resulting CG waste. The content of glyceride oil in CG seeds, peels and seed/peel waste, as well as the individual fatty acid, sterol and tocopherol composition of the oils was determined. CG seeds and seed/peel waste were a rich source of oil (up to 22.93%), which is suitable for nutritional application, due to its high proportions of unsaturated fatty acids (up to 83.77%), sterols (campesterol, Δ5-аvenasterol, β-sitosterol) and tocopherols (β-, δ- and γ-tocopherols). Seed/peel waste and the extracted seed cakes contained macro- and microminerals (K, Mg, Na, Fe, Zn, Mn, Cu) which are important for human and animal nutrition. Seed cakes had relatively high protein (24.32%) and cellulose (42.94%) contents, and an interesting amino acid profile. The results from the study contribute to a deeper understanding of the composition of CG fruit, and might be of practical relevance in the development of functional foods and feeds.El consumo del aguaymanto (Physalis peruviana L.), fresco o procesado, está ganando popularidad en todo el mundo debido a sus beneficios nutricionales y medicinales. Este estudio se basó en el análisis de la fracción lipídica de diferentes partes de la fruta y en una mayor valorización de los desechos resultantes. Se determinó el contenido de la fracción glicerídica en semillas, cáscaras y residuos de semillas/cáscaras, así como la composición individual de ácidos grasos, esteroles y tocoferoles de los aceites. Las semillas de aguaymanto y los residuos de semillas/cáscaras fueron una rica fuente de aceite (hasta 22,93%), adecuados para un uso nutricional, debido a las altas proporciones de ácidos grasos insaturados (hasta 83,77%), esteroles (campesterol, Δ5-avenasterol, β-sitosterol) y tocoferoles (β-, δ- y γ-tocoferol). Los residuos de semillas/cáscaras y los residuos desengrasados de semillas extraídos (tortas) contenían macro y microminerales (K, Mg, Na, Fe, Zn, Mn, Cu) importantes para la nutrición humana y animal. Las tortas de semillas tenían un contenido relativamente alto de proteínas (24,32%) y celulosa (42,94%), y un perfil de aminoácidos interesante. Los resultados del estudio contribuyen a una comprensión más profunda de la composición del aguaymanto y pueden ser de relevancia práctica en el desarrollo de alimentos y alimentos funcionales

Efficient Synthesis of Fluorescent Coumarins and Phosphorous-Containing Coumarin-Type Heterocycles via Palladium Catalyzed Cross-Coupling Reactions

Quantum-chemical calculations on the spectral properties of some aryl substituted 3- phosphonocoumarins were performed, and the effect of the substituents in the aryl moiety was evaluated. The structures possessing promising fluorescent properties were successfully synthesized via Suzuki and Sonogashira cross-coupling. The synthetic protocol was also applied for the phosphorous chemoisomer of 3-phosphonocoumarin, 1,2-benzoxaphosphorin, and their carboxylate analogues. The optical properties of the arylated and alkynylated products were experimentally determined. The obtained quantum-chemical and experimental results give the possibility for a fine tuning of the optical properties of phosphorous-containing coumarin systems by altering the substituent at its C-6 position

Rigidity and defect actions in Landau-Ginzburg models

Studying two-dimensional field theories in the presence of defect lines naturally gives rise to monoidal categories: their objects are the different (topological) defect conditions, their morphisms are junction fields, and their tensor product describes the fusion of defects. These categories should be equipped with a duality operation corresponding to reversing the orientation of the defect line, providing a rigid and pivotal structure. We make this structure explicit in topological Landau-Ginzburg models with potential x^d, where defects are described by matrix factorisations of x^d-y^d. The duality allows to compute an action of defects on bulk fields, which we compare to the corresponding N=2 conformal field theories. We find that the two actions differ by phases.Comment: 53 pages; v2: clarified exposition of pivotal structures, corrected proof of theorem 2.13, added remark 3.9; version to appear in CM

Boundary states and finite size effects in sine-Gordon model with Neumann boundary condition

The sine-Gordon model with Neumann boundary condition is investigated. Using the bootstrap principle the spectrum of boundary bound states is established. Somewhat surprisingly it is found that Coleman-Thun diagrams and bound state creation may coexist. A framework to describe finite size effects in boundary integrable theories is developed and used together with the truncated conformal space approach to confirm the bound states and reflection factors derived by bootstrap.Comment: Latex, 40 pages, 17 eps figures. References and remarks adde

A Monoidal Category for Perturbed Defects in Conformal Field Theory

Starting from an abelian rigid braided monoidal category C we define an abelian rigid monoidal category C_F which captures some aspects of perturbed conformal defects in two-dimensional conformal field theory. Namely, for V a rational vertex operator algebra we consider the charge-conjugation CFT constructed from V (the Cardy case). Then C = Rep(V) and an object in C_F corresponds to a conformal defect condition together with a direction of perturbation. We assign to each object in C_F an operator on the space of states of the CFT, the perturbed defect operator, and show that the assignment factors through the Grothendieck ring of C_F. This allows one to find functional relations between perturbed defect operators. Such relations are interesting because they contain information about the integrable structure of the CFT.Comment: 38 pages; v2: corrected typos and expanded section 3.2, version to appear in CM

Sister chromatid exchanges and micronuclei in peripheral lymphocytes of shoe factory workers exposed to solvents.

We examined sister chromatid exchanges (SCEs) and micronuclei (MN; cytokinesis-block method) in cultured peripheral lymphocytes from 52 female workers of two shoe factories and from 36 unexposed age- and sex-matched referents. The factory workers showed an elevated level of urinary hippuric acid, a biomarker of toluene exposure, and workplace air contained high concentrations of various organic solvents such as toluene, gasoline, acetone, and (in one of the plants only) ethylacetate and methylenediphenyl diisocyanate. The shoe factory workers showed a statistically significant higher frequency of micronucleated binucleate lymphocytes in comparison with the referents. This finding agreed with three preliminary MN determinations (each comprising 27-32 shoe workers and 16-20 controls) performed in one of the plants 2-5 years earlier. The shoe factory workers also had a lower average level of blood hemoglobin than the referents. In contrast, no difference was found between the groups in SCE analysis. Smokers showed significantly higher mean frequencies of SCEs per cell and high frequency cells (HFC) than nonsmokers. Aging was associated with increased MN rates and reduced cell proliferation. Polymorphism of the glutathione S-transferase M1 gene (GSTM1) did not affect the individual level of SCEs; but in smoking shoe workers an effect of the occupational exposure on the frequency of micronucleated cells could be seen only in GSTM1 null subjects. The low prevalence of the glutathione S-transferase T1 (GSTT1) null genotype precluded the evaluation of the influence of GSTT1 polymorphism. Our results show that the shoe factory workers have experienced genotoxic exposure, which is manifest as an increase in the frequency of MN, but not of SCEs, in peripheral lymphocytes. The exposures responsible for the MN induction could not be identified with certainty, but exposure to benzene in gasoline and methylenediphenyl diisocyanate may explain some of the findings

Thermodynamic Selection of Steric Zipper Patterns in the Amyloid Cross-β Spine

At the core of amyloid fibrils is the cross-β spine, a long tape of β-sheets formed by the constituent proteins. Recent high-resolution x-ray studies show that the unit of this filamentous structure is a β-sheet bilayer with side chains within the bilayer forming a tightly interdigitating “steric zipper” interface. However, for a given peptide, different bilayer patterns are possible, and no quantitative explanation exists regarding which pattern is selected or under what condition there can be more than one pattern observed, exhibiting molecular polymorphism. We address the structural selection mechanism by performing molecular dynamics simulations to calculate the free energy of incorporating a peptide monomer into a β-sheet bilayer. We test filaments formed by several types of peptides including GNNQQNY, NNQQ, VEALYL, KLVFFAE and STVIIE, and find that the patterns with the lowest binding free energy correspond to available atomistic structures with high accuracy. Molecular polymorphism, as exhibited by NNQQ, is likely because there are more than one most stable structures whose binding free energies differ by less than the thermal energy. Detailed analysis of individual energy terms reveals that these short peptides are not strained nor do they lose much conformational entropy upon incorporating into a β-sheet bilayer. The selection of a bilayer pattern is determined mainly by the van der Waals and hydrophobic forces as a quantitative measure of shape complementarity among side chains between the β-sheets. The requirement for self-complementary steric zipper formation supports that amyloid fibrils form more easily among similar or same sequences, and it also makes parallel β-sheets generally preferred over anti-parallel ones. But the presence of charged side chains appears to kinetically drive anti-parallel β-sheets to form at early stages of assembly, after which the bilayer formation is likely driven by energetics

X-ray Crystallographic Structure of an Artificial β-Sheet Dimer

This paper describes the X-ray crystallographic structure of a designed cyclic beta-sheet peptide that forms a well-defined hydrogen-bonded dimer that mimics beta-sheet dimers formed by proteins. The 54-membered ring macrocyclic peptide (1a) contains molecular template and turn units that induce beta-sheet structure in a heptapeptide strand that forms the dimerization interface. The X-ray crystallographic structure reveals the structures of the two "Hao" amino acids that help template the beta-sheet structure and the two delta-linked ornithine turn units that link the Hao-containing template to the heptapeptide beta-strand. The Hao amino acids adopt a conformation that resembles a tripeptide in a beta-strand conformation, with one edge of the Hao unit presenting an alternating array of hydrogen-bond donor and acceptor groups in the same pattern as that of a tripeptide beta-strand. The delta-linked ornithines adopt a conformation that resembles a hydrogen-bonded beta-turn, in which the ornithine takes the place of the i+1 and i+2 residues. The dimers formed by macrocyclic beta-sheet 1a resemble the dimers of many proteins, such as defensin HNP-3, the lambda-Cro repressor, interleukin 8, and the ribonuclease H domain of HIV-1 reverse transcriptase. The dimers of 1a self-assemble in the solid state into a barrel-shaped trimer of dimers in which the three dimers are arranged in a triangular fashion. Molecular modeling in which one of the three dimers is removed and the remaining two dimers are aligned face-to-face provides a model of the dimers of dimers of closely related macrocyclic beta-sheet peptides that were observed in solution