Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect

With the help of sepiolite, a unique method for transforming DNA into bacteria, based on the Yoshida effect, has been developed recently. However, we confronted many problems when this newest method was tried. Only a few transformants could be obtained even when 100 ng of plasmid pET15b was used, and a successful result seemed difficult to repeat. To address this problem, we optimized the operating method and could achieve about 15,000 transformants using the same amount of plasmid, which could match the efficiency gained using the calcium chloride transformation method. Meanwhile, the results could also be reproduced well. In the same way, carbon nanotubes were used to attain more than 15,000 transformants in the same situation. Therefore, the transformation method could be extended to other nanomaterials. Meanwhile, compared with the mechanism previously reported, we verified quite a different principle for the mechanism responsible for such a transformation. In sum, this unique transformation can be developed to become the third widely-used transformation method in laboratories in addition to the chemical method and electroporation

Prototyping Hexagonal Light Concentrators Using High-Reflectance Specular Films for the Large-Sized Telescopes of the Cherenkov Telescope Array

We have developed a prototype hexagonal light concentrator for the Large-Sized Telescopes of the Cherenkov Telescope Array. To maximize the photodetection efficiency of the focal-plane camera pixels for atmospheric Cherenkov photons and to lower the energy threshold, a specular film with a very high reflectance of 92-99% has been developed to cover the inner surfaces of the light concentrators. The prototype has a relative anode sensitivity (which can be roughly regarded as collection efficiency) of about 95 to 105% at the most important angles of incidence. The design, simulation, production procedure, and performance measurements of the light-concentrator prototype are reported.Comment: 21 pages, 14 figures, accepted for publication in JINS

The Biermann Battery in Cosmological MHD Simulations of Population III Star Formation

We report the results of the first self-consistent three-dimensional adaptive mesh refinement magnetohydrodynamical simulations of Population III star formation including the Biermann Battery effect. We find that the Population III stars formed including this effect are both qualitatively and quantitatively similar to those from hydrodynamics-only (non-MHD) cosmological simulations. We observe peak magnetic fields of ~10^-9 G in the center of our star-forming halo at z ~ 17.55. The magnetic fields created by the Biermann Battery effect are predominantly formed early in the evolution of the primordial halo at low density and large spatial scales, and then grow through compression and by shear flows. The fields seen in this calculation are never large enough to be dynamically important (with beta >= 10^{15} at all times), and should be considered the minimum possible fields in existence during Population III star formation, and may be seed fields for the stellar dynamo or the magnetorotational instability at higher densities and smaller spatial scales.Comment: 4 pages, 3 figures; Submitted to the Astrophysical Journal Letters. Comments welcome. Typo found (and fixed) in equation

The Birth of a Galaxy: Primordial Metal Enrichment and Stellar Populations

By definition, Population III stars are metal-free, and their protostellar collapse is driven by molecular hydrogen cooling in the gas-phase, leading to large characteristic masses. Population II stars with lower characteristic masses form when the star-forming gas reaches a critical metallicity of 10^{-6} - 10^{-3.5} Z_\odot. We present an adaptive mesh refinement radiation hydrodynamics simulation that follows the transition from Population III to II star formation. The maximum spatial resolution of 1 comoving parsec allows for individual molecular clouds to be well-resolved and their stellar associations to be studied in detail. We model stellar radiative feedback with adaptive ray tracing. A top-heavy initial mass function for the Population III stars is considered, resulting in a plausible distribution of pair-instability supernovae and associated metal enrichment. We find that the gas fraction recovers from 5 percent to nearly the cosmic fraction in halos with merger histories rich in halos above 10^7 solar masses. A single pair-instability supernova is sufficient to enrich the host halo to a metallicity floor of 10^{-3} Z_\odot and to transition to Population II star formation. This provides a natural explanation for the observed floor on damped Lyman alpha (DLA) systems metallicities reported in the literature, which is of this order. We find that stellar metallicities do not necessarily trace stellar ages, as mergers of halos with established stellar populations can create superpositions of t-Z evolutionary tracks. A bimodal metallicity distribution is created after a starburst occurs when the halo can cool efficiently through atomic line cooling.Comment: 11 pages, 7 figures; replaced with accepted version to ApJ; additional movies and images can be found at http://www.astro.princeton.edu/~jwise/research/GalaxyBirth.htm

Generation of Seed Magnetic Field around First Stars: the Biermann Battery Effect

We investigate generation processes of magnetic fields around first stars. Since the first stars are expected to form anisotropic ionization fronts in the surrounding clumpy media, magnetic fields are generated by effects of radiation force as well as the Biermann battery effect. We have calculated the amplitude of magnetic field generated by the effects of radiation force around the first stars in the preceding paper, in which the Biermann battery effects are not taken into account.In this paper, we calculate the generation of magnetic fields by the Biermann battery effect as well as the effects of radiation force, utilizing the radiation hydrodynamics simulations. As a result, we find that the generated magnetic field strengths are ~ 10^{-19}G-10^{-17}G at ~ 100pc-1kpc scale mainly by the Biermann battery, which is an order of magnitude larger than the results of our previous study. We also find that this result is insensitive to various physical parameters including the mass of the source star, distance between the source and the dense clump, unless we take unlikely values of these parameters.Comment: 19 pages, 7 figures, Accepted for publications in Ap

Suppression of HD-cooling in protogalactic gas clouds by Lyman-Werner radiation

It has been shown that HD molecules can form efficiently in metal-free gas collapsing into massive protogalactic halos at high redshift. The resulting radiative cooling by HD can lower the gas temperature to that of the cosmic microwave background, T_CMB=2.7(1+z)K, significantly below the temperature of a few 100 K achievable via H_2-cooling alone, and thus reduce the masses of the first generation of stars. Here we consider the suppression of HD-cooling by UV irradiation in the Lyman-Werner (LW) bands. We include photo-dissociation of both H_2 and HD, and explicitly compute the self-shielding and shielding of both molecules by neutral hydrogen as well as the shielding of HD by H_2. We use a simplified dynamical collapse model, and follow the chemical and thermal evolution of the gas, in the presence of a UV background. We find that a LW flux of J_crit = 1e-22 erg/cm^2/sr/s/Hz is able to suppress HD cooling and thus prevent collapsing primordial gas from reaching temperatures below 100 K. The main reason for the lack of HD cooling for J>J_crit is the partial photo-dissociation of H_2, which prevents the gas from reaching sufficiently low temperatures (T<150K) for HD to become the dominant coolant; direct HD photo-dissociation is unimportant except for a narrow range of fluxes and column densities. Since the prevention of HD-cooling requires only partial H_2 photo-dissociation, the critical flux J_crit is modest, and is below the UV background required to reionize the universe at redshift z=10-20. We conclude that HD-cooling can reduce the masses of typical stars only in rare halos forming well before the epoch of reionization.Comment: 14 pages with 9 figures, submitted to MNRA

The Effects of Accretion Luminosity upon Fragmentation in the Early Universe

We introduce a prescription for the luminosity from accreting protostars into smoothed particle hydrodynamics simulation, and apply the method to simulations of five primordial minihalos generated from cosmological initial conditions. We find that accretion luminosity delays fragmentation within the halos, but does not prevent it. In halos that slowly form a low number of protostars, the accretion luminosity can reduce the number of fragments that are formed before the protostars start ionising their surroundings. However, halos that rapidly form many protostars become dominated by dynamical processes, and the effect of accretion luminosity becomes negligible. Generally the fragmentation found in the halos is highly dependent on the initial conditions. Accretion luminosity does not substantially affect the accretion rates experienced by the protostars, and is far less important than dynamical interactions, which can lead to ejections that effectively terminate the accretion. We find that the accretion rates onto the inner regions of the disks (20 AU) around the protostars are highly variable, in contrast to the constant or smoothly decreasing accretion rates currently used in models of the pre-main sequence evolution of Population III stars.Comment: 12 pages, 10 figures and 3 tables. Accepted by MNRA

Formation of the seed black holes: a role of quark nuggets?

Strange quark nuggets (SQNs) could be the relics of the cosmological QCD phase transition, and they could very likely be the candidate of cold quark matter if survived the cooling of the later Universe, although the formation and evolution of these SQNs depend on the physical state of the hot QGP (quark-gluon plasma) phase and the state of cold quark matter. We reconsider the possibility of SQNs as cold dark matter, and find that the formation of black holes in primordial halos could be significantly different from the standard scenario. In a primordial halo, the collision between gas and SQNs could be frequent enough, and thus the viscosity acting on each SQN would decrease its angular momentum and make it to sink into the center of the halo, as well as heat the gas. The SQNs with baryon numbers less than $10^{35}$ could assemble in the center of the halo before the formation of primordial stars. A black hole could form by merger of these SQNs, and then its mass could quickly become about $10^3\ M_\odot$ or higher, by accreting the surrounding SQNs or gas. The black holes formed in this way could be the seeds for the supermassive black holes at redshift as high as $z\sim 6$.Comment: 15 page

The first stars: formation of binaries and small multiple systems

We investigate the formation of metal-free, Population III (Pop III), stars within a minihalo at z ~ 20 with a smoothed particle hydrodynamics (SPH) simulation, starting from cosmological initial conditions. Employing a hierarchical, zoom-in procedure, we achieve sufficient numerical resolution to follow the collapsing gas in the center of the minihalo up to number densities of 10^12 cm^-3. This allows us to study the protostellar accretion onto the initial hydrostatic core, which we represent as a growing sink particle, in improved physical detail. The accretion process, and in particular its termination, governs the final masses that were reached by the first stars. The primordial initial mass function (IMF), in turn, played an important role in determining to what extent the first stars drove early cosmic evolution. We continue our simulation for 5000 yr after the first sink particle has formed. During this time period, a disk-like configuration is assembled around the first protostar. The disk is gravitationally unstable, develops a pronounced spiral structure, and fragments into several other protostellar seeds. At the end of the simulation, a small multiple system has formed, dominated by a binary with masses ~ 40 M_Sun and ~ 10 M_Sun. If Pop III stars were to form typically in binaries or small multiples, the standard model of primordial star formation, where single, isolated stars are predicted to form in minihaloes, would have to be modified. This would have crucial consequences for the observational signature of the first stars, such as their nucleosynthetic pattern, and the gravitational-wave emission from possible Pop III black-hole binaries.Comment: Accepted to MNRAS. New section with new figure added. 18 pages, 13 figures. Supplementary material and high resolution version at http://www.as.utexas.edu/~minerva