Houetal_Supplementary Data 1

Supposed protaspid and meraspides of Zhangshania typica from the Hongjingshao Formation, in Kunming, Yunnan Province, southern China: (1), supposed protaspid of this species, YKLP 12249; (2), same specimen of YKLP 12249 photographed under different lighting; (3), Meraspid degree 2, YKLP 12250; (4), enlargement of pygidium of specimen YKLP 12250; (5), Meraspid degree 3, YKLP 12251; (6), enlargement of pygidium of specimen YKLP 12251. p, pygidial segment; t, thoracic segment. Same abbreviations seen below. Scale bars are (1, 2, 4, 6) 200 µm; (3, 5) 500 µm

Houetal_Supplementary Data 3

Meraspides and early holaspides of Zhangshania typica from the Hongjingshao Formation, in Kunming, Yunnan Province, southern China: (1), Meraspid degree 10, YKLP 12319; (2), Meraspid degree 11, YKLP 12259; (3), Meraspid degree 12, YKLP 12260; (4), Meraspid degree 13, YKLP 12261; (5), enlargement of pygidium of specimen YKLP 12261; (6), Holaspid stage 1, YKLP 12262; (7), enlargement of pygidium of specimen YKLP 12262; (8), Holaspid stage 2, YKLP 12263; (9), enlargement of pygidium of specimen YKLP 12263. Scale bars are (5, 7, 9) 200 µm; (1–3) 500 µm; (4, 6, 8) 1.0 mm

Data from: Ontogeny of the articulated yiliangellinine trilobite Zhangshania typica from the lower Cambrian (Series 2, Stage 3) of southern China

New discoveries of the early Cambrian yiliangellinine trilobite Zhangshania typica Li and Zhang in Kunming preserve almost all instars from early postembryonic (protaspid) to mature (holaspid) phases in articulated state, in addition to mature specimens with antennae bearing paired spines on the basal articles. The ontogenetic series shows protarthrous development with some, but likely not all, early holaspid instars expressing additional pygidial segments, gradual rearward migration of the location of the longest pleural spines on the trunk segments, and striking positive allometry of the genal spines. It also reveals Parazhangshania sichuanensis Li and Zhang, 1990 to be the holaspid stage 3 of Z. typica, and therefore its junior synonym. This new find in the Hongjingshao Formation provides species-based regional correlation across the South China block and Z. typica may provide an important biostratigraphic marker for the base of the traditional Tsanglangpuan Stage

Houetal_Supplementary Data 2

Meraspides of Zhangshania typica from the Hongjingshao Formation, in Kunming, Yunnan Province, southern China: (1), Meraspid degree 4, YKLP 12252; (2), enlargement of cephalon of specimen YKLP 12252; (3), enlargement of pygidium of specimen YKLP 12252; (4), Meraspid degree 5, YKLP 12253;(5), Meraspid degree 6, YKLP 12254; (6), enlargement of pygidium of specimen YKLP 12254; (7), Meraspid degree 7, YKLP 12255; (8), enlargement of pygidium of specimen YKLP 12255; (9), Meraspid degree 8, YKLP 12256; (10), enlargement of pygidium of specimen YKLP 12256; (11), Meraspid degree 9, YKLP 12257; (12), enlargement of pygidium of specimen YKLP 12257. Scale bars are (3, 6, 8, 10, 12) 200 µm; (1–2, 4–5) 500 µm; (7, 9, 11) 1.0 mm

Identification of PP2A/Set binding sites and design of interacting peptides with potential clinical applications

Protein phosphatase 2A (PP2A) is known to be a negative regulator of several survival and proliferating pathways that are frequently altered in cancer. In addition to chemical enzymatic inhibitors of the PP2A activity, the oncoprotein SET has been described as a physiological PP2A inhibitor by forming a complex with PP2A catalytic subunit (PP2Ac). Increased SET protein levels therefore directly reduce the tumor suppressor function of PP2A and promote tumor progression. We have used the PEP-Scan approach to identify the binding site between the serine/threonine phosphatase PP2A and the oncoprotein SET. For in vivo validation of the peptides, we have used chronic lymphocytic leukemia (CLL) xenograft models. In this manuscript we describe the identification of amino acid sequences involved in the complex formation, both at the PP2Ac and SET sides. The amino acid sequences of the binding sites were coupled to an optimized penetrating peptide in order to generate chimeras (Mut3DPT-PP2A and Mut3DPT-SET) able to target the PP2A/SET interaction. We demonstrate that these peptides have an in vitro apoptotic effect on breast and lung cancer cell lines, as well as an antitumoral effect on CLL and lymphoma xenograft models. The new generated chimeric peptides allow the modulation of the PP2Ac/SET interaction and might have a potential as a new therapeutic approach for cancer treatment.status: publishe