113 research outputs found
Diversity of thermal responses of lipid composition in the membranes of the dominant culturable members of an Antarctic fellfield soil bacterial community
- Publication venue
- 'Cambridge University Press (CUP)'
- Publication date
- 01/09/2000
- Field of study
The eight dominant culturable members of an Antarctic fellfield soil bacterial community were four Arthrobacter species, Sanguibacter suarezii, Aureobacterium testaceum, a Bacillus sp., and a Pseudomonas sp.. All of the isolates grew at 2°C, but two of the Arthrobacter spp. were psychrophilic, while the other six bacterial species were psychrotolerant. However, the fastest growing organisms at low temperatures were not the psychrophiles, and the psychrotolerant Bacillus sp. grew fastest at temperatures up to 25°C. When the growth temperature of cultures was altered, the phospholipid content of the two psychrophilic Arthrobacter spp. decreased, whereas the phospholipid contents of the psychrotolerant spp. either increased or did not change. Only one psychrophilic and one psychrotolerant Arthrobacter sp. modified its polar lipid head-group composition in response to a lowering of growth temperature. The change in Arthrobacter sp. CL2-1 was particularly marked and novel in that at low temperatures phosphatidylethanolamine was replaced completely by a phosphoglycolipid and phosphatidylserine, neither of which was present at higher growth temperatures. All eight isolates altered the fatty acyl compositions of their membrane lipids in a manner that was only partially dependent on taxonomic status. In Bacillus sp. C2-1 the changes were opposite to that predicted on the basis of membrane fluidity considerations. The isolates used different combinations of changes in fatty acid branching, unsaturation and chain length. There was no single strategy of thermal adaptation that was employed and the variety of strategies used did not follow phylogenetic boundaries
Combinatorial hydrogel library enables identification of materials that mitigate the foreign body response in primates
- Author
- A Bernhardt
- A King
- A Omer
- A Omer
- A Rodriguez
- Adam C Graham
- Alan Chiu
- AM Rokstad
- Andrew R Bader
- Arturo J Vegas
- B Manoury
- BD Ratner
- BE Tuch
- BJ de Haan
- CJ Kearney
- D Jacobs-Tulleneers-Thevissen
- Dale L Greiner
- Daniel G Anderson
- David M Harlan
- DF Williams
- DW Grainger
- DW Scharp
- EM Hetrick
- EM Sussman
- Erin Langan
- F Lim
- G Orive
- G Wick
- GA Paredes-Juarez
- GA Paredes-Juarez
- GC Weir
- Gordon C Weir
- Hok Hei Tam
- J Dusseault
- J Haller
- James McGarrigle
- Jennifer Hollister-Locke
- Jeon Woong Kang
- JH Brauker
- Jie Li
- JL Harding
- JM Anderson
- Jose Oberholzer
- Josh Cohen
- Joshua C Doloff
- JW Kang
- JW Kang
- K Hofman
- K Kim
- Kaitlin Bratlie
- Karolina Siniakowicz
- Karsten Olejnik
- Katherine Tang
- KM Bratlie
- KY Lee
- L Zhang
- LR Madden
- M Gu
- M Liu
- M Ménard
- M Qi
- M Sato
- Matthew A Bochenek
- Meirigeng Qi
- Michael Chen
- Minglin Ma
- Nimit Dholakia
- O Veiseh
- Omid Veiseh
- P de Vos
- P de Vos
- Patrick Fenton
- R Kharb
- R Langer
- R Lindstedt
- R Robitaille
- Raj Thakrar
- Robert Langer
- S Brocchini
- Sean Siebert
- Siddharth Jhunjhunwala
- Stephanie Aresta-Dasilva
- Stephen Lyle
- T Christen
- TA Wynn
- Thema Vietti
- TT Dang
- VF Duvivier-Kali
- WK Ward
- WL Stoppel
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- 01/02/2015
- Field of study
The foreign body response is an immune-mediated reaction that can lead to the failure of implanted medical devices and discomfort for the recipient. There is a critical need for biomaterials that overcome this key challenge in the development of medical devices. Here we use a combinatorial approach for covalent chemical modification to generate a large library of variants of one of the most widely used hydrogel biomaterials, alginate. We evaluated the materials in vivo and identified three triazole-containing analogs that substantially reduce foreign body reactions in both rodents and, for at least 6 months, in non-human primates. The distribution of the triazole modification creates a unique hydrogel surface that inhibits recognition by macrophages and fibrous deposition. In addition to the utility of the compounds reported here, our approach may enable the discovery of other materials that mitigate the foreign body response.Leona M. and Harry B. Helmsley Charitable Trust (3-SRA-2014-285-M-R)United States. National Institutes of Health (EB000244)United States. National Institutes of Health (EB000351)United States. National Institutes of Health (DE013023)United States. National Institutes of Health (CA151884)United States. National Institutes of Health (P41EB015871-27)National Cancer Institute (U.S.) (P30-CA14051
Interpretative and predictive modelling of Joint European Torus collisionality scans
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- Abduallev S.
- Abhangi M.
- Abreu P.
- Afanasev V.
- Afzal M.
- Aggarwal K. M.
- Ahlgren T.
- Aho-Mantila L.
- Aiba N.
- Airila M.
- Alarcon T.
- Albanese R.
- Alegre D.
- Aleiferis S.
- Alessi E.
- Aleynikov P.
- Alkseev A.
- Allinson M.
- Alper B.
- Alves E.
- Ambrosino G.
- Ambrosino R.
- Amosov V.
- Andrews R.
- Angelone M.
- Anghel M.
- Angioni C.
- Appel L.
- Appelbee C.
- Arena P.
- Ariola M.
- Arshad S.
- Artaud J.
- Arter W.
- Asakura N. N.
- Ash A.
- Ashikawa N.
- Aslanyan V.
- Asunta O.
- Asztalos O.
- Auriemma F.
- Austin Y.
- Avotina L.
- Axton M.
- Ayres C.
- Baciero A.
- Baiao D.
- Balboa I.
- Balden M.
- Balshaw N.
- Bandaru V. K.
- Banks J.
- Baranov Y. F.
- Barcellona C.
- Barnard T.
- Barnes M.
- Barnsley R.
- Baruzzo M.
- Basiuk V.
- Bassan M.
- Bastow R.
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- Zoita V.
- Zoletnik S.
- Zwingmann W.
- Zychor I.
- Publication venue
- IOP Publishing
- Publication date
- 17/04/2020
- Field of study
Transport modelling of Joint European Torus (JET) dimensionless collisionality scaling experiments in various operational scenarios is presented. Interpretative simulations at a fixed radial position are combined with predictive JETTO simulations of temperatures and densities, using the TGLF transport model. The model includes electromagnetic effects and collisions as well as □(→┬E ) X □(→┬B ) shear in Miller geometry. Focus is on particle transport and the role of the neutral beam injection (NBI) particle source for the density peaking. The experimental 3-point collisionality scans include L-mode, and H-mode (D and H and higher beta D plasma) plasmas in a total of 12 discharges. Experimental results presented in (Tala et al 2017 44th EPS Conf.) indicate that for the H-mode scans, the NBI particle source plays an important role for the density peaking, whereas for the L-mode scan, the influence of the particle source is small. In general, both the interpretative and predictive transport simulations support the experimental conclusions on the role of the NBI particle source for the 12 JET discharges
Effect of the relative shift between the electron density and temperature pedestal position on the pedestal stability in JET-ILW and comparison with JET-C
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- Tommaso Bonanomi
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- Ul Abidin
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- V. Zoletnik
- Vallejos Olivares
- Van De Mortel
- Van Eester
- Van Renterghem
- Van Rooij
- Verona Rinati
- Viola B.
- W. W. Wakeling
- W. Brombin
- W. Day
- W. Hemming
- W. Li
- W. Subba
- W. Tardocchi
- W. Yao
- W. Zhou
- X. Abduallev
- X. Gao
- X. Leggate
- Y. F. Barnard
- Y. S. Nabais
- Y. Avotina
- Y. Camp
- Y. Corrigan
- Y. Garcia
- Y. Hatch
- Y. K\ue3\ub6ppen
- Y. Kazantzidis
- Y. Likonen
- Y. Marcenko
- Y. Maruyama
- Y. Mlyn\ue3\ua1\ue5\u2122
- Y. Pfefferl\ue3\ua9
- Y. Zilli
- Z. Ghate
- Z. Uljanovs
- Z. Vlad
- Publication venue
- 'IOP Publishing'
- Publication date
- 01/01/2018
- Field of study
The electron temperature and density pedestals tend to vary in their relative radial positions, as observed in DIII-D (Beurskens et al 2011 Phys. Plasmas 18 056120) and ASDEX Upgrade (Dunne et al 2017 Plasma Phys. Control. Fusion 59 14017). This so-called relative shift has an impact on the pedestal magnetohydrodynamic (MHD) stability and hence on the pedestal height (Osborne et al 2015 Nucl. Fusion 55 063018). The present work studies the effect of the relative shift on pedestal stability of JET ITER-like wall (JET-ILW) baseline low triangularity (\u3b4) unseeded plasmas, and similar JET-C discharges. As shown in this paper, the increase of the pedestal relative shift is correlated with the reduction of the normalized pressure gradient, therefore playing a strong role in pedestal stability. Furthermore, JET-ILW tends to have a larger relative shift compared to JET carbon wall (JET-C), suggesting a possible role of the plasma facing materials in affecting the density profile location. Experimental results are then compared with stability analysis performed in terms of the peeling-ballooning model and with pedestal predictive model EUROPED (Saarelma et al 2017 Plasma Phys. Control. Fusion). Stability analysis is consistent with the experimental findings, showing an improvement of the pedestal stability, when the relative shift is reduced. This has been ascribed mainly to the increase of the edge bootstrap current, and to minor effects related to the increase of the pedestal pressure gradient and narrowing of the pedestal pressure width. Pedestal predictive model EUROPED shows a qualitative agreement with experiment, especially for low values of the relative shift
Cohort Profile: Post-Hospitalisation COVID-19 (PHOSP-COVID) study
- Author
- Abel K
- Adamali H
- Adeloye D
- Adeyemi O
- Adrego R
- Ahmad S
- Ahmed R
- Ahwireng N
- Ainsworth M
- Al-Sheklly B
- Alamoudi A
- Ali M
- Aljaroof M
- All AM
- Allan L
- Allen RJ
- Allerton L
- Allsop L
- Almeida P
- Altmann D
- Amoils S
- Anderson D
- Anifowose S
- Antoniades C
- Arbane G
- Arias A
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- Armour C
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- Armstrong N
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- Arnold H
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- Aslani S
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- Atkin C
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- Publication venue
- Oxford University Press (OUP)
- Publication date
- 18/12/2023
- Field of study
Gapping as constituent coordination
- Author
- Anna Szabolcsi
- Anna Szabolcsi
- Anna Szabolcsi
- Anneke Neijt
- Anthony E. Ades
- Barbara Abbott
- Beom-Mo Kang
- Crit Cremers
- D. Terence Langendoen
- David A. Turner
- David A. Turner
- David Dowly
- Deirdre Wilson
- Edwin Williams
- Edwin Williams
- Emmon Bach
- Eva Haji?ová
- Eva Haji?ová
- Franz Zwarts
- Gerald Gazdar
- Gerald Gazdar
- Gerald Gazdar
- Gosse Bouma
- Gosse Bouma
- Graham Mallinson
- Grant Goodall
- Haskell B. Curry
- Ivan Sag
- Ivan Sag
- Joachim Lambek
- Joachim Lambek
- Joan M Maling
- Johan Benthem van
- John Robert Ross
- John Robert Ross
- Jorge Hankamer
- Joseph Aoun
- Joseph Greenberg
- Justine T. Stillings
- Kazimierz Ajdukiewicz
- Lauri Karttunen
- Lila Gleitman
- Liliane Haegeman
- Lisa Selkirk
- M. A. K Halliday
- M. Wood
- Maria Luisa Zubizarreta
- Mark J Steedman
- Mark J Steedman
- Mark J. Steedman
- Mark J. Steedman
- Michael Moortgat
- Michael Moortgat
- Muffy Siegel
- Nico Zee van der
- Noam Chomsky
- Noam Chomsky
- Noam Chomsky
- Noam Chomsky
- Paul Schachter
- Paul Schachter
- Paul Schacter
- Pauline Jacobson
- Peter T Geach
- Ray Jackendoff
- Ray Jackendoff
- Raymond Smullyan
- Richard Hudson
- Richard T Oehrle
- Robert R. Oirsouw van
- Robert R. Oirsouw van
- Roland Hausser
- Stuart Shieber
- Stuart Shieber
- Susumo Kuno
- W Zielonka
- Wynn Chao
- Yehoshua Bar-Hillel
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Effects of fluoxetine on functional outcomes after acute stroke (FOCUS): a pragmatic, double-blind, randomised, controlled trial
- Author
- Abano N
- Abbott L
- Abbott W
- Abdul-Hamid A
- Abdul-Saheb M
- Abousleiman Y
- Abubakar S
- Adedoyin T
- Adie K
- Affley B
- Ahlquist K
- Ahmad N
- Ahmed A
- Ahmed S
- Ahmed Z
- Al Hussayni S
- Al-Samarrai N
- Alam I
- Alam S
- Ali A
- Ali K
- Allen C
- Allen J
- Allison J
- Allsop H
- Allsop L
- Almadenboyle C
- Alvares W
- Alwis L
- Alwis L
- Amero J
- Amis E
- Amlani S
- Amor K
- Anazodo C
- Anderson R
- Andrews J
- Anjum T
- Annamalai A
- Annison F
- Anthony A
- Appiatse G
- Archer J
- Argandona L
- Arif S
- Armer C
- Armstrong L
- Aruldoss P
- Arundell L-L
- Ashcroft P
- Asokanathan A
- Aubrey B
- Augustin M
- Auld G
- Austin D
- Awolesi J
- Ayer J
- Azim A
- Badiani B
- Bahk A
- Bailey D
- Bailey L
- Bakawala R
- Baker J
- Baker P
- Balami J
- Balasubramanian V
- Balian L
- Ball J
- Ball L
- Balogun I
- Bamford E
- Bamford J
- Banaras A
- Barber M
- Barbon E
- Barcroft H
- Barker J
- Barron C
- Barron L
- Barry A
- Barugh A
- Bate J
- Bateman G
- Bates M
- Bathula R
- Beadle H
- Beadling T
- Beard R
- Beardmore C
- Bedford C
- Bedford E
- Begum Y
- Bell A
- Bell J
- Bell J
- Bell S
- Bell S
- Bellfield R
- Benford A
- Bent C
- Benton L
- Beranova E
- Beranova E
- Besley S
- Bhalla A
- Bhandari M
- Bharaj K
- Bhargava M
- Bhattad M
- Bhome G
- Billingham S
- Birns J
- Black T
- Blackburn J
- Blackburn R
- Blair G
- Blane S
- Blight A
- Blight A
- Bloom S
- Board J
- Board S
- Bohannan E
- Bokhari M
- Bolton M
- Bond K
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- Borley J
- Borley J
- Boulton A
- Boulton R
- Bower R
- Bowers R
- Bowie E
- Bowler H
- Bowring A
- Box C
- Bradfield S
- Bradshaw E
- Brady M
- Brammer S
- Bray H
- Breeds J
- Brewer C
- Brezitski M
- Bridger H
- Brighouse-Johnson C
- Brodie C
- Brodie F
- Brookes L
- Brotheridge S
- Brotherton L
- Broughton D
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- Buchanan D
- Buckley C
- Bunworth K
- Burgess D
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- Burgess S
- Burgoyne J-R
- Burn I
- Burn M
- Burnip R
- Burns A
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- Burova M
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- Butt G
- Bwalya A
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- Cain R
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- Cape A
- Carpenter M
- Carpio R
- Carr L
- Carson S
- Carter T
- Cartwright B
- Cassidy T
- Cattermole E
- Causley C
- Ceccarelli D
- Chadbourn I
- Chadha D
- Chakrabarti A
- Chamberlain A
- Chan K
- Chandler S
- Chandrasena D
- Chaplin S
- Chapman N
- Chapman R
- Chapter T
- Charles B
- Chatterjee K
- Chattha N
- Chauhan M
- Chembala J
- Cheripelli B
- Chesser D
- Chin Y-M
- Chinery E
- Chohan O
- Chopra N
- Choy L
- Christie D
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- Christmas N
- Chun Y
- Clark Brown P
- Clarke B
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- Clarke R
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- Clarkson E
- Claydon F
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- Cloud G
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- Coburn I
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- Publication venue
- 'Elsevier BV'
- Publication date
- 01/01/2019
- Field of study
Background
Results of small trials indicate that fluoxetine might improve functional outcomes after stroke. The FOCUS trial aimed to provide a precise estimate of these effects.
Methods
FOCUS was a pragmatic, multicentre, parallel group, double-blind, randomised, placebo-controlled trial done at 103 hospitals in the UK. Patients were eligible if they were aged 18 years or older, had a clinical stroke diagnosis, were enrolled and randomly assigned between 2 days and 15 days after onset, and had focal neurological deficits. Patients were randomly allocated fluoxetine 20 mg or matching placebo orally once daily for 6 months via a web-based system by use of a minimisation algorithm. The primary outcome was functional status, measured with the modified Rankin Scale (mRS), at 6 months. Patients, carers, health-care staff, and the trial team were masked to treatment allocation. Functional status was assessed at 6 months and 12 months after randomisation. Patients were analysed according to their treatment allocation. This trial is registered with the ISRCTN registry, number ISRCTN83290762.
Findings
Between Sept 10, 2012, and March 31, 2017, 3127 patients were recruited. 1564 patients were allocated fluoxetine and 1563 allocated placebo. mRS data at 6 months were available for 1553 (99·3%) patients in each treatment group. The distribution across mRS categories at 6 months was similar in the fluoxetine and placebo groups (common odds ratio adjusted for minimisation variables 0·951 [95% CI 0·839–1·079]; p=0·439). Patients allocated fluoxetine were less likely than those allocated placebo to develop new depression by 6 months (210 [13·43%] patients vs 269 [17·21%]; difference 3·78% [95% CI 1·26–6·30]; p=0·0033), but they had more bone fractures (45 [2·88%] vs 23 [1·47%]; difference 1·41% [95% CI 0·38–2·43]; p=0·0070). There were no significant differences in any other event at 6 or 12 months.
Interpretation
Fluoxetine 20 mg given daily for 6 months after acute stroke does not seem to improve functional outcomes. Although the treatment reduced the occurrence of depression, it increased the frequency of bone fractures. These results do not support the routine use of fluoxetine either for the prevention of post-stroke depression or to promote recovery of function.
Funding
UK Stroke Association and NIHR Health Technology Assessment Programme
Post-acute COVID-19 neuropsychiatric symptoms are not associated with ongoing nervous system injury
- Author
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- Zongo O.
- Publication venue
- Oxford University Press
- Publication date
- 27/12/2023
- Field of study
A proportion of patients infected with severe acute respiratory syndrome coronavirus 2 experience a range of neuropsychiatric symptoms months after infection, including cognitive deficits, depression and anxiety. The mechanisms underpinning such symptoms remain elusive. Recent research has demonstrated that nervous system injury can occur during COVID-19. Whether ongoing neural injury in the months after COVID-19 accounts for the ongoing or emergent neuropsychiatric symptoms is unclear. Within a large prospective cohort study of adult survivors who were hospitalized for severe acute respiratory syndrome coronavirus 2 infection, we analysed plasma markers of nervous system injury and astrocytic activation, measured 6 months post-infection: neurofilament light, glial fibrillary acidic protein and total tau protein. We assessed whether these markers were associated with the severity of the acute COVID-19 illness and with post-acute neuropsychiatric symptoms (as measured by the Patient Health Questionnaire for depression, the General Anxiety Disorder assessment for anxiety, the Montreal Cognitive Assessment for objective cognitive deficit and the cognitive items of the Patient Symptom Questionnaire for subjective cognitive deficit) at 6 months and 1 year post-hospital discharge from COVID-19. No robust associations were found between markers of nervous system injury and severity of acute COVID-19 (except for an association of small effect size between duration of admission and neurofilament light) nor with post-acute neuropsychiatric symptoms. These results suggest that ongoing neuropsychiatric symptoms are not due to ongoing neural injury
Long COVID and cardiovascular disease: a prospective cohort study
- Author
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- Publication venue
- BMJ
- Publication date
- 27/05/2024
- Field of study
Background
Pre-existing cardiovascular disease (CVD) or cardiovascular risk factors have been associated with an increased risk of complications following hospitalisation with COVID-19, but their impact on the rate of recovery following discharge is not known.
Objectives
To determine whether the rate of patient-perceived recovery following hospitalisation with COVID-19 was affected by the presence of CVD or cardiovascular risk factors.
Methods
In a multicentre prospective cohort study, patients were recruited following discharge from the hospital with COVID-19 undertaking two comprehensive assessments at 5 months and 12 months. Patients were stratified by the presence of either CVD or cardiovascular risk factors prior to hospitalisation with COVID-19 and compared with controls with neither. Full recovery was determined by the response to a patient-perceived evaluation of full recovery from COVID-19 in the context of physical, physiological and cognitive determinants of health.
Results
From a total population of 2545 patients (38.8% women), 472 (18.5%) and 1355 (53.2%) had CVD or cardiovascular risk factors, respectively. Compared with controls (n=718), patients with CVD and cardiovascular risk factors were older and more likely to have had severe COVID-19. Full recovery was significantly lower at 12 months in patients with CVD (adjusted OR (aOR) 0.62, 95% CI 0.43 to 0.89) and cardiovascular risk factors (aOR 0.66, 95% CI 0.50 to 0.86).
Conclusion
Patients with CVD or cardiovascular risk factors had a delayed recovery at 12 months following hospitalisation with COVID-19. Targeted interventions to reduce the impact of COVID-19 in patients with cardiovascular disease remain an unmet need
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