The High Energy Emission of the Crab Nebula from 20 keV to 6 MeV with INTEGRAL

The SPI spectrometer aboard the INTEGRAL mission observes regularly the Crab Nebula since 2003. We report on observations distributed over 5.5 years and investigate the variability of the intensity and spectral shape of this remarkable source in the hard X-rays domain up to a few MeV. While single power law models give a good description in the X-ray domain (mean photon index ~ 2.05) and MeV domain (photon index ~ 2.23), crucial information are contained in the evolution of the slope with energy between these two values. This study has been carried out trough individual observations and long duration (~ 400 ks) averaged spectra. The stability of the emission is remarkable and excludes a single power law model. The slopes measured below and above 100 keV agree perfectly with the last values reported in the X-ray and MeV regions respectively, but without indication of a localized break point. This suggests a gradual softening in the emission around 100 keV and thus a continuous evolution rather than an actual change in the mechanism parameters. In the MeV region, no significant deviation from the proposed power law model is visible up to 5-6 MeV. Finally, we take advantage of the spectroscopic capability of the instrument to seek for previously reported spectral features in the covered energy range with negative results for any significant cyclotron or annihilation emission on 400 ks timescales. Beyond the scientific results, the performance and reliability of the SPI instrument is explicitly demonstrated, with some details about the most appropriate analysis method.Comment: accepted for publication in ApJ; 4 figures, 2 table

Dual functions of Macpiwi1 in transposon silencing and stem cell maintenance in the flatworm Macrostomum lignano

PIWI proteins and piRNA pathways are essential for transposon silencing and some aspects of gene regulation during animal germline development. In contrast to most animal species, some flatworms also express PIWIs and piRNAs in somatic stem cells, where they are required for tissue renewal and regeneration. Here, we have identified and characterized piRNAs and PIWI proteins in the emerging model flatworm Macrostomum lignano. We found that M. lignano encodes at least three PIWI proteins. One of these, Macpiwi1, acts as a key component of the canonical piRNA pathway in the germline and in somatic stem cells. Knockdown of Macpiwi1 dramatically reduces piRNA levels, derepresses transposons, and severely impacts stem cell maintenance. Knockdown of the piRNA biogenesis factor Macvasa caused an even greater reduction in piRNA levels with a corresponding increase in transposons. Yet, in Macvasa knockdown animals, we detected no major impact on stem cell self-renewal. These results may suggest stem cell maintenance functions of PIWI proteins in flatworms that are distinguishable from their impact on transposons and that might function independently of what are considered canonical piRNA populations

SPI Measurements of Galactic 26Al

The precision measurement of the 1809 keV gamma-ray line from Galactic $^{26}$Al is one of the goals of the SPI spectrometer on INTEGRAL with its Ge detector camera. We aim for determination of the detailed shape of this gamma-ray line, and its variation for different source regions along the plane of the Galaxy. Data from the first part of the core program observations of the first mission year have been inspected. A clear detection of the \Al line at about 5--7 $\sigma$ significance demonstrates that SPI will deepen \Al studies. The line intensity is consistent with expectations from previous experiments, and the line appears narrower than the 5.4 keV FWHM reported by GRIS, more consistent with RHESSI's recent value. Only preliminary statements can be made at this time, however, due to the multi-component background underlying the signal at \about 40 times higher intensity than the signal from Galactic $^{26}$Al.Comment: 5 pages, 8 figures; accepted for publication in A&A (special INTEGRAL volume

SPI/INTEGRAL observation of the Cygnus region

We present the analysis of the first observations of the Cygnus region by the SPI spectrometer onboard the Integral Gamma Ray Observatory, encompassing ${\sim}$ 600 ks of data. Three sources namely Cyg X-1, Cyg X-3 and EXO 2030+375 were clearly detected. Our data illustrate the temporal variability of Cyg X-1 in the energy range from 20 keV to 300 keV. The spectral analysis shows a remarkable stability of the Cyg X-1 spectra when averaged over one day timescale. The other goal of these observations is SPI inflight calibration and performance verification. The latest objective has been achieved as demonstrated by the results presented in this paper.Comment: 6 pages, 10 figures, accepted for publication in A&A (special INTEGRAL volume

Re-structuring of marine communities exposed to environmental change

Species richness is the most commonly used but controversial biodiversity metric in studies on aspects of community stability such as structural composition or productivity. The apparent ambiguity of theoretical and experimental findings may in part be due to experimental shortcomings and/or heterogeneity of scales and methods in earlier studies. This has led to an urgent call for improved and more realistic experiments. In a series of experiments replicated at a global scale we translocated several hundred marine hard bottom communities to new environments simulating a rapid but moderate environmental change. Subsequently, we measured their rate of compositional change (re-structuring) which in the great majority of cases represented a compositional convergence towards local communities. Re-structuring is driven by mortality of community components (original species) and establishment of new species in the changed environmental context. The rate of this re-structuring was then related to various system properties. We show that availability of free substratum relates negatively while taxon richness relates positively to structural persistence (i.e., no or slow re-structuring). Thus, when faced with environmental change, taxon-rich communities retain their original composition longer than taxon-poor communities. The effect of taxon richness, however, interacts with another aspect of diversity, functional richness. Indeed, taxon richness relates positively to persistence in functionally depauperate communities, but not in functionally diverse communities. The interaction between taxonomic and functional diversity with regard to the behaviour of communities exposed to environmental stress may help understand some of the seemingly contrasting findings of past research

HDAC9 is implicated in atherosclerotic aortic calcification and affects vascular smooth muscle cell phenotype.

Aortic calcification is an important independent predictor of future cardiovascular events. We performed a genome-wide association meta-analysis to determine SNPs associated with the extent of abdominal aortic calcification (n = 9,417) or descending thoracic aortic calcification (n = 8,422). Two genetic loci, HDAC9 and RAP1GAP, were associated with abdominal aortic calcification at a genome-wide level (P < 5.0 × 10-8). No SNPs were associated with thoracic aortic calcification at the genome-wide threshold. Increased expression of HDAC9 in human aortic smooth muscle cells promoted calcification and reduced contractility, while inhibition of HDAC9 in human aortic smooth muscle cells inhibited calcification and enhanced cell contractility. In matrix Gla protein-deficient mice, a model of human vascular calcification, mice lacking HDAC9 had a 40% reduction in aortic calcification and improved survival. This translational genomic study identifies the first genetic risk locus associated with calcification of the abdominal aorta and describes a previously unknown role for HDAC9 in the development of vascular calcification

Electrical coupling of neuro-ommatidial photoreceptor cells in the blowfly

A new method of microstimulation of the blowfly eye using corneal neutralization was applied to the 6 peripheral photoreceptor cells (R1-R6) connected to one neuro-ommatidium (and thus looking into the same direction), whilst the receptor potential of a dark-adapted photoreceptor cell was recorded by means of an intracellular microelectrode. Stimulation of the photoreceptor cells not impaled elicited responses in the recorded cell of about 20% of the response elicited when stimulating the recorded cell. This is probably caused by gap junctions recently found between the axon terminals of these cells. Stimulation of all 6 cells together yielded responses that were larger and longer than those obtained with stimulation of just the recorded cell, and intensity-response curves that deviated more strongly from linearity. Evidence is presented that the resistance of the axon terminal of the photoreceptor cells quickly drops in response to a light flash, depending on the light intensity. Incorporating the cable properties of the cell body and the axon, the resistance of the gap junctions, and the (adapting) terminal resistance, a theoretical model is presented that explains the measurements well. Finally, it is argued that the gap junctions between the photoreceptor cells may effectively uncouple the synaptic responses of the cells by counteracting the influence of field potentials.

SPI: The spectrometer aboard INTEGRAL

SPI is a high spectral resolution gamma-ray telescope on board the ESA mission INTEGRAL (International Gamma Ray Astrophysics Laboratory). It consists of an array of 19 closely packed germanium detectors surrounded by an active anticoincidence shield of BGO. The imaging capabilities of the instrument are obtained with a tungsten coded aperture mask located 1.7 m from the Ge array. The fully coded field-of-view is 16º, the partially coded field of view amounts to 31º, and the angular resolution is 2.5º. The energy range extends from 20 keV to 8 MeV with a typical energy resolution of 2.5 keV at 1.3 MeV. Here we present the general concept of the instrument followed by a brief description of each of the main subsystems. INTEGRAL was successfully launched in October 2002 and SPI is functioning extremely well.Sanchez Martinez, Filomeno, [email protected]