Tissue-specific Expression of Distinct Spectrin and Ankyrin Transcripts in Erythroid and Nonerythroid Cells

cDNA probes for three components of the erythroid membrane skeleton, α spectrin, β spectrin, and ankyrin, were obtained by using monospecific antibodies to screen a λgt11 expression vector library containing cDNA prepared from chicken erythroid poly(A)^+ RNA. Each cDNA appears to hybridize to one gene type in the chicken genome. Qualitatively distinct RNA species in myogenic and erythroid cells are detected for β spectrin and ankyrin, while α spectrin exists as a single species of transcript in all tissues examined. This tissue-specific expression of RNAs is regulated quantitatively during myogenesis in vitro, since all three accumulate only upon myoblast fusion. Furthermore, RNAs for two of the three genes do not accumulate to detectable levels in chicken embryo fibroblasts, demonstrating that their accumulation can be noncoordinate. These observations suggest that independent gene regulation and tissue-specific production of heterogeneous transcripts from the β spectrin and ankyrin genes underlie the formation of distinct membrane skeletons in erythroid and muscle cells

Alternative news on social media in Norway

Alternative news media in Norway have become visible in public debates. Partly because of news sharing on social media. Social media has become an arena for news, information, and public debate, and has also become a place to fight for the news agenda. The present study examines news sharing in social media in Norway and how right-wing alternative news outlets use social media to impact the news agenda. These are small organizations with only a handful of employees, but they have very proactive readers and feature a considerable amount of user-generated content. They are critical of immigration, particularly from Muslim countries, and of the political elite. They mimic traditional media in the way they organize and label their content, but their reporting is more subjective. The present study uses quantitative content analysis to reveal which topics are the most shared on social media, and from which news type of news organizations they come. It also looks at how news sharing differed in the days following a series of terrorist attacks and how the pattern changed during the course of a normal day. This leads to a discussion on participatory journalism and how news sharing can be seen as a part of the public debate

Introduction and Expression of a Rabbit β-globin Gene in Mouse Fibroblasts

The cloned chromosomal rabbit ß-globin gene has been introduced into mouse fibroblasts by DNA-mediated gene transfer (transformation). In this report, we examine the expression of the rabbit gene in six independent transformants that contain from 1 to 20 copies of the cloned globin gene. Rabbit globin transcripts were detected in two of these transformants at steady-state concentrations of 5 and 2 copies per cell. The globin transcripts from one cell line are polyadenylylated and migrate as 9S RNA on methylmercury gels. These transcripts reflect correct processing of the two intervening sequences but lack 48 ± 5 nucleotides present at the 5' terminus of rabbit erythrocyte globin mRNA

The planar two-body problem for spheroids and disks

We outline a new method suggested by Conway (2016) for solving the two-body problem for solid bodies of spheroidal or ellipsoidal shape. The method is based on integrating the gravitational potential of one body over the surface of the other body. When the gravitational potential can be analytically expressed (as for spheroids or ellipsoids), the gravitational force and mutual gravitational potential can be formulated as a surface integral instead of a volume integral, and solved numerically. If the two bodies are infinitely thin disks, the surface integral has an analytical solution. The method is exact as the force and mutual potential appear in closed-form expressions, and does not involve series expansions with subsequent truncation errors. In order to test the method, we solve the equations of motion in an inertial frame, and run simulations with two spheroids and two infinitely thin disks, restricted to torque-free planar motion. The resulting trajectories display precession patterns typical for non-Keplerian potentials. We follow the conservation of energy and orbital angular momentum, and also investigate how the spheroid model approaches the two cases where the surface integral can be solved analytically, i.e. for point masses and infinitely thin disks.Comment: 15 pages, 5 figures, accepted for publication in Celestial Mechanics and Dynamical Astronom

Antioxidant activity in commonly grown and consumed vegetables: a screening survey

A diet rich in fruits and vegetables is associated with a reduced risk of developing cardiovascular diseases and certain types of cancer. This positive effect is related to bioactive phytochemicals found in plants.The vegetables were grown in the field or in greenhouses at the Norwegian University of Life Sciences (59º40’N) during the years 2000-2002. The vegetables were harvested at commercial maturity and analysed for dry matter and antioxidant activity assessed by the FRAP (Ferric Reducing Ability of Plasma) assay. There was a large variation in antioxidant activity both between and within different species. The highest antioxidant activity was observed in kale, red cultivars of cabbage and table beet. The lowest antioxidant activity was observed in lettuce, cucumber, carrots and tomato. The vegetables possessing a red colour showed higher antioxidant activity with the exception of carrots and tomatoes

Extended two-body problem for rotating rigid bodies

A new technique that utilizes surface integrals to find the force, torque and potential energy between two non-spherical, rigid bodies is presented. The method is relatively fast, and allows us to solve the full rigid two-body problem for pairs of spheroids and ellipsoids with 12 degrees of freedom. We demonstrate the method with two dimensionless test scenarios, one where tumbling motion develops, and one where the motion of the bodies resemble spinning tops. We also test the method on the asteroid binary (66391) 1999 KW4, where both components are modelled either as spheroids or ellipsoids. The two different shape models have negligible effects on the eccentricity and semi-major axis, but have a larger impact on the angular velocity along the $z$-direction. In all cases, energy and total angular momentum is conserved, and the simulation accuracy is kept at the machine accuracy level.Comment: 24 pages, 9 figures, accepted for publication in Celestial Mechanics and Dynamical Astronom

JHK Observations of Faint Standard Stars in the Mauna Kea Near-Infrared Photometric System

JHK photometry in the Mauna Kea Observatory (MKO) near-IR system is presented for 115 stars. Of these, 79 are UKIRT standards and 42 are LCO standards. The average brightness is 11.5 mag, with a range of 10 to 15. The average number of nights each star was observed is 4, and the average of the internal error of the final results is 0.011 mag. These JHK data agree with those reported by other groups to 0.02 mag. The measurements are used to derive transformations between the MKO JHK photometric system and the UKIRT, LCO and 2MASS systems. The 2MASS-MKO data scatter by 0.05 mag for redder stars: 2MASS-J includes H2O features in dwarfs and MKO-K includes CO features in giants. Transformations derived for stars whose spectra contain only weak features cannot give accurate transformations for objects with strong absorption features within a filter bandpasses. We find evidence of systematic effects at the 0.02 mag level in the photometry of stars with J<11 and H,K<10.5. This is due to an underestimate of the linearity correction for stars observed with the shortest exposure times; very accurate photometry of stars approaching the saturation limits of infrared detectors which are operated in double-read mode is difficult to obtain. Four stars in the sample, GSPC S705-D, FS 116 (B216-b7), FS 144 (Ser-EC84) and FS 32 (Feige 108), may be variable. 84 stars in the sample have 11< J< 15 and 10.5<H,K<15, are not suspected to be variable, and have magnitudes with an estimated error <0.027 mag; 79 of these have an error of <0.020 mag. These represent the first published high-accuracy JHK stellar photometry in the MKO photometric system; we recommend these objects be employed as primary standards for that system [abridged].Comment: Accepted for publication in MNRAS, 14 pages, 5 Figure

XMM-Newton Surveys of the Canada-France Redshift Survey Fields - III: The Environments of X-ray Selected AGN at 0.4<z<0.6

The environmental properties of a sample of 31 hard X-ray selected AGN are investigated, from scales of 500 kpc down to 30 kpc, and are compared to a control sample of inactive galaxies. The AGN all lie in the redshift range 0.4<z<0.6. The accretion luminosity-density of the Universe peaks close to this redshift range, and the AGN in the sample have X-ray luminosities close to the knee in the hard X-ray luminosity function, making them representative of the population which dominated this important phase of energy conversion. Using both the spatial clustering amplitude and near neighbour counts it is found that the AGN have environments that are indistinguishable from normal, inactive galaxies over the same redshift range and with similar optical properties. Typically, the environments are of sub-cluster richness, in contrast to similar studies of high-z quasars, which are often found in clusters with comparable richness to the Abell R>=0 clusters. It is suggested that minor mergers with low mass companions is a likely candidate for the mechanism by which these modest luminosity AGN are fuelled.Comment: 12 pages, 6 figures, accepted by MNRA

Mega-Environment Targeting of Maize Varieties using Ammi and GGE Bi-Plot Analysis in Ethiopia

አህፅሮት በቆሎ በኢትዮጵያ  ከሚመረቱ የምግብ ሰብሎች መካከል በምርትና ምርታማነቱ ግንባር ቀደም ስፍራን የያዘ ሰብል ነው፡፡  የሰብሉን ምርታማነት ከሚደግፉ የተለያዩ መንስዔዎች  ውስጥ በዋናነት ከፍተኛውን  ቦታ  የሚይዙት ከጥናትና ምርምር  የተገኙ የተሻሻሉ ዝርያዎች ቢሆኑም ሁሉም ዝርያዎች   በበቆሎ አብቃይ ስነ-ምህዳሮች  ላይ ተዘርተው  በምርታማነታቸው ወጥነት የማያሳዩ መሆናቸው ይታወቃል፡፡ እንደየአካባቢው የአይር ፀባይ፤ የአፈር ዓይነትና የዝናብ መጠን እንዲሁም የመሬት ከባህር ወለል ከፍታ ልዩነት የተነሳ በምርታማነታቸው ለየአካባቢው ተመራጭና ተመራጭ ያልሆኑ ዝርያዎችን መለየት ይቻላል፡፡ በዚህ ምክንያት ለተለያዩ ዝርያዎች ምርታማነት ተስማሚና ወካይ የሆኑ ስፍራዎችን  ለይቶ በማወቅ የትኛው ዝርያ በየትኛው ስፍራ ላይ ቢዘራ  ሁለንተናዊ የአካባቢ ባህሪያትን  ተላብሶ ከፍተኛ ምርት ሊሰጥ ይችላል?  እንዲሁም የትኛቹ ስፍራዎች በአየር ንብረት ተቀራራቢነት በጥቅል ተደምረው አንድ ዝርያ በወጥነት  በሁሉም ስፍራ ተዘርቶ ምርታማ የሚያደርጋቸውን አካባቢዎች ለይቶ ለማወቅ ጥናቱ ተደረገ፡፡ ጥናቱ ለምርት በምርምር የተለቀቁ  19 ዲቃላ የበቆሎ ዝርያዎችን በማካተት  ወይናደጋማና ደጋማ ስፍራዎች ላይ ተዘርተው የተለያዩ መረጃዎችን  በማሰባሰብ እንዲጠናቀር ከተደረገ በኋላ ለጥናቱ ስኬት   ከፍተኛ ትኩረት ተሰጥቶት  ለውሳኔ  እንዲያመች ከየአካባቢው የተሰበሰቡ የዝርያዎቹ ምርት አግባብ ባላቸው ሳይንሳዊ ዘዴዎች እንዲሰሉ ተደረገ፡፡ በስሌቱ መሰረት ከዝርያዎቹ በአማካይ በሔክታር 4.47 ( BH545)  እስከ 7.49  ( BH546) ቶን  ምርት ተመዘገበ፡፡ እንዲሁም በተደረገው ስሌት G14  እና  G1  ተብለው የተለዩ ዝርያዎች ለአብዛኞቹ የጥናቱ ስፍራዎች ተስማሚ  እንደሆኑ  ቢታወቅም  BH546  በሚባል ስያሜ የሚለየው ዝርያ በከፍተኛ ደረጃ ተመራጭ እንደሆነ ለማረጋገጥ ተችለሏል፡፡ በሌላ በኩል E9  በተባለ ምህፃረ-ቃል የሚለይ ስፍራ በአብዛኛው ዝርያዎች  ተመራጭ እንደሆነ ስሌቱ ሲያሳይ ፤ E1  የተባለው ግን ተመራጭ እንዳልሆነ ታውቋል፡፡ ሆኖም ግን 11 የጥናት ስፍራዎች በሶስት ዋና ዋና ፤ እያንዳንዳቸው በዝርዎቹ ምርታማነት የጎላ ልዩነት በሚታይባቸው ወጥ ክፍሎች እንደተከፈሉ የስሌቱ ውጤት ለይቶ አሳይቷል፡፡ በዚህ መሰረት E9 በሚል ስያሜ የሚለየው ስፍራ በብቸኝንት እንደ አንድ ዋና ክፍል የተከፈለ ሲሆን በሁለተኛ  ክፍል ውስጥ  በጥቅል  ዘጠኝ  አካባቦዎች  E1, E2, E3, E5, E6, E7, E8  እና E11  በአንድነት ተደመሩ፤ እንዲሁም  E4 እና  E10 በሶስተኛው ክፍል ውስጥ ተመደቡ፡፡ E3, E5 and, E7 በተባሉ ምህፃረ-ቃል የተለዩ ስፍራዎች ለዝርዎቹ ምርታማነት ወካይና ተመራጭ መሆናቸውን ጥናቱ አሳየ፡፡  ነገር ግን E4, E9 and E10  የተባሉ አካባቢዎች በውስን ስፍራዎች ውስጥ  ምርታማ የሚሆኑ  ዝርያዎችን መለየት የሚችሉ መሆናቸውን ጥናቱ ያረጋግጣል፡፡  በሌላ በኩል E8 and E11 የተባሉ ስፍራዎች የዝርያዎችን ምርታማነትና ተመራጭነት  በጉልህ ለማሳየት ምንም አስተዋፅዖ ያላበረከቱ መሆናቸውን ጥናቱ አሳይቷል፡፡ በመጨረሻም የዚህ ጥናት ውጤት ወጥነት ያላቸው ሶስት ዋና ዋና ስነ-ምህዳራትን ለይቷል፤  ዝርይዎች በምርታማነታቸው   ተመራጭነት  የሚኖራቸውንና  የማይኖራቸውነ  ለይተው የሚያሳዩ ስፍራዎችን  ጠቁሟል እንዲሁም በምርታማነቱና ለአብዛኛው አካባቢዎች  በወጥነት ተስማሚነቱን የሜያሳይ ዝርያ ለይቶ አሳይቷል፡፡ Abstract In multi-location experimental trials, test locations must be selected to properly discriminate between varieties and to be representative of the target regions. The objective of this study were to evaluate test locations in terms of discrimination ability, representativeness, and desirability, and to investigate the presence of mega-environments using AMMI and GGE models and to suggest representative environments for breeding and variety testing purposes.  Among 19 maize varieties tested across 11 environments, mean grain yield ranged between 4.47 t/ha (BH545) to 7.49 t/ha (BH546). Both AMMI and GGE  models identified   G14 and G1 as  desirable hybrids for cultivation   because they combined stability and higher average yield. Nonetheless, as confirmed by GGE analysis BH546 was most closest to the ideal genotype hence, considered as best hybrid.  Environment wise, E9 and E4 were the most stable and unstable test environments, respectively. The 11 test environments fell into three apparent mega-environments.  E9 formed one group by its own, E1, E2, E3, E5, E6, E7, E8 and E11 formed the second group and E4 and E10 formed the third group.  E3, E5 and, E7 were both discriminating and representative therefore are favorable environments for selecting generally adapted genotypes. E4, E9 and E10 were discriminating but non-representative test environments thus are useful for selecting specifically adapted genotypes. E8 and E11 were non-discriminating test environments hence little information about the genotypes. The results of this study helped to identify mega-environments, also representativeness and discriminating power of test environments better visualized with the GGE bi-plot model