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Vibrational spectroscopic investigations of lead borate and lead aluminoborate glasses
Lead borate (PB) and lead aluminoborate (PBA) glasses in the Systems xPbO · ( 1 0 0 - x)Β₂O₃ (0 ≤ x ≤ 75 mol%) and xPbO · (95 - x)Β₂O₃, 5AI₂O₃ (0 ≤ x ≤ 80 mol%), respectively, have been prepared and investigated by Raman and infrared reflectance spectroscopy to obtain quahtative and quantitative information on the structure of glasses. Using the Martin-Brenig model, structural correlation lengths were determinated from boson peak positions of temperature-reduced Raman spectra and transversal sound velocities. Kramers-Kronig analysis was used to transform the measured infrared reflectance spectra into complex dielectric funcdons. The imaginary part of the complex dielectric funcdon contains the absorption properties of t he material. Α band separadon Computer programme has been used to get more detailed information from the spectra. Far infrared and low-frequency Raman spectra contain information about vibrations of the PbO component within the network
In Situ Raman Investigation on the Structural Transformation of Oxovanadium Hydrogenphosphate Hemihydrate in the Presence of Ammonia
Anti-Apoptotic Function of Gelsolin in Fas Antibody-Induced Liver Failure in Vivo
Apoptosis is a key mechanism underlying fulminant hepatic failure. The role of gelsolin in such apoptotic pathways is not well understood because both pro-apoptotic and anti-apoptotic effects have been reported in vitro, depending on the cell type and in vitro expression model used. Therefore, we studied an in vivo model of hepatic failure by analyzing expression of gelsolin; intrahepatic activation of caspase-3, -8, and -9; and the extent of apoptosis in gelsolin knockout (gsn(−/−)) versus wild-type mice (gsn(+/+)) after exposure to stimulatory Fas antibody Jo-2. Gelsolin was expressed exclusively in sinusoidal lining cells, including sinusoidal endothelial cells and Kupffer cells, of gsn(+/+) mice. Compared with wild-type mice, Jo2-exposed gsn(−/−) mice showed significantly higher numbers of apoptotic cells in the liver (22 ± 9 versus 5 ± 4% terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling-positive cells, P = 0.002); shorter survival (P = 0.037); and enhanced activation of caspase-3 (P = 0.009), -8 (P = 0.004), and -9 (P = 0.004). Furthermore, inhibition of caspase-3 with z-DEVD-fmk blocked Jo2-induced liver failure in all mice. Thus, our data on Jo2-induced hepatic failure suggest that gelsolin exerts an overall anti-apoptotic effect in vivo. Moreover, selective expression of gelsolin in sinusoidal endothelial cells indicates a pivotal role for interactions between sinusoidal endothelial cells and liver parenchymal cells in Fas ligand-mediated liver failure